##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233103_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46727462 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.765333627578574 31.0 30.0 34.0 27.0 34.0 2 31.04400050659717 31.0 31.0 34.0 28.0 34.0 3 30.868128831820567 31.0 31.0 34.0 27.0 34.0 4 33.11794826348583 35.0 33.0 37.0 25.0 37.0 5 33.77580427971885 35.0 33.0 37.0 28.0 37.0 6 34.36667936298359 35.0 35.0 37.0 30.0 37.0 7 34.76312297894545 35.0 35.0 37.0 32.0 37.0 8 34.81985653318813 35.0 35.0 37.0 32.0 37.0 9 36.36242725958452 38.0 35.0 39.0 32.0 39.0 10-11 36.245151202519835 38.0 35.0 39.0 31.5 39.0 12-13 36.18621127978233 38.0 35.0 39.0 31.5 39.0 14-15 37.34798131128971 39.0 36.0 41.0 32.0 41.0 16-17 37.33501486770243 39.0 36.0 41.0 32.0 41.0 18-19 37.33080791976248 39.0 36.0 41.0 31.5 41.0 20-21 37.27605533123113 39.0 36.0 41.0 31.0 41.0 22-23 37.19694813512449 39.0 36.0 41.0 31.0 41.0 24-25 37.08594893298506 39.0 36.0 41.0 31.0 41.0 26-27 36.71705778713169 39.0 36.0 40.0 30.0 41.0 28-29 36.52067142015973 39.0 35.5 40.0 30.0 41.0 30-31 36.35541918583124 39.0 35.0 40.0 30.0 41.0 32-33 36.20813618124605 39.0 35.0 40.0 30.0 41.0 34-35 36.13286294042677 38.0 35.0 40.0 29.5 41.0 36-37 35.99375246402212 38.0 35.0 40.0 29.0 41.0 38-39 35.87616885761953 38.0 35.0 40.0 29.0 41.0 40-41 35.71537445153773 38.0 34.5 40.0 27.5 41.0 42-43 35.48158637419682 38.0 34.0 40.0 27.0 41.0 44-45 35.32951716915419 38.0 34.0 40.0 27.0 41.0 46-47 35.13622680812409 38.0 34.0 40.0 26.5 41.0 48-49 34.941400423160154 38.0 33.0 40.0 26.0 41.0 50-51 34.993535985327 38.0 33.5 40.0 26.0 41.0 52-53 34.85991931254473 38.0 33.5 40.0 26.0 41.0 54-55 34.78123087446949 38.0 33.5 40.0 26.0 41.0 56-57 34.53124825183101 38.0 33.0 40.0 25.0 41.0 58-59 34.31370794330751 37.5 33.0 40.0 24.5 41.0 60-61 34.02967380295553 37.0 33.0 40.0 23.5 41.0 62-63 33.731667835929116 37.0 32.5 40.0 23.0 41.0 64-65 33.43025763950116 36.0 32.0 40.0 22.5 41.0 66-67 33.174260470213426 36.0 32.0 39.0 22.0 41.0 68-69 32.89902663662752 36.0 31.0 39.0 22.0 40.5 70-71 32.561547297390135 35.0 31.0 39.0 21.5 40.0 72-73 32.20076236967461 35.0 31.0 38.0 20.5 40.0 74-75 31.83752558613177 35.0 31.0 37.0 20.0 39.5 76-77 30.79483363551823 34.0 29.5 36.0 19.0 39.0 78-79 31.102274653821347 34.0 30.0 36.0 19.0 39.0 80-81 30.958138417618315 34.0 30.0 36.0 18.0 39.0 82-83 30.71798515399788 34.0 30.0 36.0 18.0 37.0 84-85 30.392928670082703 34.0 30.0 35.0 17.0 37.0 86-87 30.03368997443088 34.0 30.0 35.0 12.0 36.5 88-89 29.697469027956195 34.0 29.5 35.0 8.5 36.0 90-91 29.46715984702957 34.0 29.0 35.0 7.0 36.0 92-93 29.242914680450653 34.0 29.0 35.0 2.0 36.0 94-95 28.963369270943925 34.0 29.0 35.0 2.0 35.0 96-97 28.626841074741016 34.0 29.0 35.0 2.0 35.0 98-99 28.083430317700543 34.0 29.0 35.0 2.0 35.0 100-101 26.412588939668925 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0251796959057913 1101 2 1.0800506634195592 1101 3 1.2796999805554954 1101 4 2.5339169402795747 1101 5 1.8460743142762581 1101 6 1.2578013770912406 1101 7 0.7792730425903827 1101 8 0.7040423275645082 1101 9 0.7566603756747696 1101 10-11 0.7996187213963353 1101 12-13 0.9064088367769827 1101 14-15 1.1341229038410816 1101 16-17 1.0292224005607693 1101 18-19 0.8813211933582181 1101 20-21 0.8665436293374924 1101 22-23 0.771355872529945 1101 24-25 0.8104481804298231 1101 26-27 0.8583440341673523 1101 28-29 0.9992159942141683 1101 30-31 1.0545849311444684 1101 32-33 1.1169136523544836 1101 34-35 0.9821082761882423 1101 36-37 0.88311076699312 1101 38-39 0.7620815151834677 1101 40-41 0.77767781766741 1101 42-43 0.8326526231398006 1101 44-45 0.84971654786888 1101 46-47 0.7792929206658812 1101 48-49 0.749694594219676 1101 50-51 0.6880539582416603 1101 52-53 0.8589369051653151 1101 54-55 0.914842124405638 1101 56-57 0.8713270839003044 1101 58-59 0.832117027303461 1101 60-61 0.828597484626215 1101 62-63 0.873540855211381 1101 64-65 0.8574246698174761 1101 66-67 0.6902940467663115 1101 68-69 0.7686185078332954 1101 70-71 0.7301316952722416 1101 72-73 0.6761585013698621 1101 74-75 0.6092786707144917 1101 76-77 0.3860123382068963 1101 78-79 0.5784862658804926 1101 80-81 0.5696691021339291 1101 82-83 0.595940586589407 1101 84-85 0.6197524392576383 1101 86-87 0.6120276326710687 1101 88-89 0.5673499426894715 1101 90-91 0.4967894825707049 1101 92-93 0.4242182682216047 1101 94-95 0.3186178351811719 1101 96-97 0.3189611386683957 1101 98-99 0.3757617781430831 1101 100-101 0.30712650847619827 1102 1 0.7191686938781778 1102 2 0.9161123399613622 1102 3 1.1829417939631348 1102 4 1.0526192721904266 1102 5 1.3147658069210308 1102 6 0.8553253210088059 1102 7 0.6904594681855727 1102 8 0.6107409130134869 1102 9 0.6620986099583916 1102 10-11 0.769703788792576 1102 12-13 0.8418830049693824 1102 14-15 1.085052207863491 1102 16-17 0.9060013683014105 1102 18-19 0.7125171632374929 1102 20-21 0.757325147504119 1102 22-23 0.7469061524683056 1102 24-25 0.7164211818101407 1102 26-27 0.7847803740840718 1102 28-29 0.9031910423514873 1102 30-31 1.0377322916778766 1102 32-33 1.1153714085179658 1102 34-35 1.0511911012142505 1102 36-37 0.8040368446304456 1102 38-39 0.8300359075584396 1102 40-41 0.6672223828521595 1102 42-43 0.7411839446426782 1102 44-45 0.8268554207786067 1102 46-47 0.837818847115436 1102 48-49 0.8410250710297333 1102 50-51 0.7846619305542788 1102 52-53 0.9046689166919393 1102 54-55 0.9630400502656613 1102 56-57 0.9295098174762728 1102 58-59 0.888000790762959 1102 60-61 0.8724054391184595 1102 62-63 0.867418369824108 1102 64-65 0.9300928964118782 1102 66-67 0.869944584565097 1102 68-69 0.8299179625502546 1102 70-71 0.8326128646569302 1102 72-73 0.8012268761704817 1102 74-75 0.7633255878145171 1102 76-77 0.5966684674274312 1102 78-79 0.7461058471410134 1102 80-81 0.6283814952301441 1102 82-83 0.6951953324579776 1102 84-85 0.6889678956869858 1102 86-87 0.5961989840029993 1102 88-89 0.532003893704946 1102 90-91 0.45984274677754655 1102 92-93 0.43544158701008584 1102 94-95 0.5113963602332063 1102 96-97 0.5336401759493477 1102 98-99 0.6013632158642359 1102 100-101 0.5682870881303295 1103 1 0.0933687034871511 1103 2 0.580731744938074 1103 3 0.8139928394115579 1103 4 -2.149083088684609 1103 5 0.17749158536571485 1103 6 0.3913741058255127 1103 7 0.39470341560168976 1103 8 0.4795268061603224 1103 9 0.5484810042818395 1103 10-11 0.6471512416856555 1103 12-13 0.6992214086329298 1103 14-15 0.884251747847749 1103 16-17 0.7459796162917698 1103 18-19 0.6451588553262226 1103 20-21 0.7715618909183277 1103 22-23 0.696665773704872 1103 24-25 0.5965508613355652 1103 26-27 0.6806386543458416 1103 28-29 0.7175941768133924 1103 30-31 0.7874644079940651 1103 32-33 0.8427560049173763 1103 34-35 0.8371022326089985 1103 36-37 0.6631431065544788 1103 38-39 0.6568002348779984 1103 40-41 0.630383119700781 1103 42-43 0.7284717066472481 1103 44-45 0.8114093299675531 1103 46-47 0.8074878233855642 1103 48-49 0.7122849029620539 1103 50-51 0.4852365104447358 1103 52-53 0.7481792022911478 1103 54-55 0.9458797795056881 1103 56-57 0.8100653937700102 1103 58-59 0.8116075973775949 1103 60-61 0.7785504422577176 1103 62-63 0.7081934041026372 1103 64-65 0.6799039183568283 1103 66-67 0.6658732883782719 1103 68-69 0.7532198553842875 1103 70-71 0.7993802111497317 1103 72-73 0.7445184480107301 1103 74-75 0.7017609514849603 1103 76-77 0.5836718755538399 1103 78-79 0.6991925809530208 1103 80-81 0.558128803739308 1103 82-83 0.712296079065311 1103 84-85 0.7560339155118108 1103 86-87 0.6838150490247976 1103 88-89 0.486191275598852 1103 90-91 0.5371940433616089 1103 92-93 0.4538899877949234 1103 94-95 0.4936568774546828 1103 96-97 0.45587511311399354 1103 98-99 0.5511902068483074 1103 100-101 0.4677691195765483 1104 1 0.47145816335954294 1104 2 0.7365508811975054 1104 3 0.7828524347682801 1104 4 0.33055361394308136 1104 5 1.0309895839286654 1104 6 0.7264461985726385 1104 7 0.5022821323155924 1104 8 0.39390427243350246 1104 9 0.4621101433004142 1104 10-11 0.5208067028974384 1104 12-13 0.5912796761826158 1104 14-15 0.780907193877816 1104 16-17 0.6833005537369132 1104 18-19 0.5370290780430906 1104 20-21 0.6569341406866798 1104 22-23 0.5830349583720604 1104 24-25 0.4986718978130753 1104 26-27 0.5929587777093559 1104 28-29 0.6448564074777039 1104 30-31 0.7328782456975418 1104 32-33 0.8238973270252288 1104 34-35 0.6852019942372181 1104 36-37 0.5402380449767605 1104 38-39 0.45192492867568035 1104 40-41 0.5515210937592343 1104 42-43 0.6458775943726565 1104 44-45 0.6394851310182261 1104 46-47 0.6044687153671404 1104 48-49 0.5401071136884639 1104 50-51 0.49704276536935055 1104 52-53 0.5903766737880503 1104 54-55 0.5791039182682169 1104 56-57 0.64550433072678 1104 58-59 0.6776452749262276 1104 60-61 0.6325974927396416 1104 62-63 0.6251507031061934 1104 64-65 0.548448608573203 1104 66-67 0.5509837196733898 1104 68-69 0.5420568519386464 1104 70-71 0.5040325512730703 1104 72-73 0.4913263876523857 1104 74-75 0.5110455933800502 1104 76-77 0.21734030131662152 1104 78-79 0.4487416377909419 1104 80-81 0.4316555992186615 1104 82-83 0.4726689460628464 1104 84-85 0.5260703410505663 1104 86-87 0.5572634044694276 1104 88-89 0.576833435750526 1104 90-91 0.4638160987794713 1104 92-93 0.3720717750991902 1104 94-95 0.37306643006279927 1104 96-97 0.3805596247333689 1104 98-99 0.42031411060205315 1104 100-101 0.35294099409031787 1105 1 -0.05559351296880877 1105 2 0.465637844440856 1105 3 -0.04086701520464686 1105 4 -1.296422135013934 1105 5 -0.5021858233683929 1105 6 0.01788552734225135 1105 7 0.2536604704701233 1105 8 0.2455122683517672 1105 9 0.3413286197022032 1105 10-11 0.3965150883005819 1105 12-13 0.5724834548858766 1105 14-15 0.7446463454561112 1105 16-17 0.6987900503655666 1105 18-19 0.5704960004186646 1105 20-21 0.5685411062132388 1105 22-23 0.5454540155182102 1105 24-25 0.4273671248715871 1105 26-27 0.542868566763758 1105 28-29 0.5746825660674659 1105 30-31 0.6831001418850704 1105 32-33 0.7853312480847165 1105 34-35 0.6947359290140298 1105 36-37 0.5722325694320674 1105 38-39 0.5684737336314143 1105 40-41 0.6056156035381548 1105 42-43 0.6723834604934851 1105 44-45 0.720922413970662 1105 46-47 0.6736242967980459 1105 48-49 0.6100903217392855 1105 50-51 0.5605356942722395 1105 52-53 0.6816316329838443 1105 54-55 0.7089058819049541 1105 56-57 0.6309396611250051 1105 58-59 0.6413730861685636 1105 60-61 0.5633660467059443 1105 62-63 0.6204213972476396 1105 64-65 0.5977855470580664 1105 66-67 0.5579142825983823 1105 68-69 0.5540787944621357 1105 70-71 0.5844189719040287 1105 72-73 0.5968953814329367 1105 74-75 0.47160461913315643 1105 76-77 0.2792651138900979 1105 78-79 0.46870182568565966 1105 80-81 0.4064973143295951 1105 82-83 0.49065749249608004 1105 84-85 0.5320152217837695 1105 86-87 0.48328824627015976 1105 88-89 0.4139590195884679 1105 90-91 0.40488746852671653 1105 92-93 0.3695716620668321 1105 94-95 0.4396627219994578 1105 96-97 0.3930316793161417 1105 98-99 0.43492723811136713 1105 100-101 0.3598130849887866 1106 1 -0.5047916466143576 1106 2 0.17378156032679826 1106 3 -1.7745919824736802 1106 4 -2.8510954902307866 1106 5 -0.4025129647799659 1106 6 -0.015006564014292678 1106 7 0.14844690241882574 1106 8 0.2047017111430165 1106 9 0.31535944133386806 1106 10-11 0.40243901066731524 1106 12-13 0.5438188343939956 1106 14-15 0.6697831195622044 1106 16-17 0.5138040451631269 1106 18-19 0.4816664091412477 1106 20-21 0.5311992767850668 1106 22-23 0.516589838324073 1106 24-25 0.3853676501616192 1106 26-27 0.46950237253053473 1106 28-29 0.5370177401045311 1106 30-31 0.6804522990095094 1106 32-33 0.7219575071598996 1106 34-35 0.5008283911336378 1106 36-37 0.5288488684754498 1106 38-39 0.50580604078894 1106 40-41 0.4442849937358986 1106 42-43 0.5051911652786814 1106 44-45 0.635445044404058 1106 46-47 0.5912530908698201 1106 48-49 0.5097869588600332 1106 50-51 0.5300972582797314 1106 52-53 0.7278547995744802 1106 54-55 0.6984744129091638 1106 56-57 0.7048114240310355 1106 58-59 0.6465885965051328 1106 60-61 0.564727420317638 1106 62-63 0.5438784141739603 1106 64-65 0.6501044854376801 1106 66-67 0.6084693570155224 1106 68-69 0.5822941745763544 1106 70-71 0.5906098855077602 1106 72-73 0.5863195374462649 1106 74-75 0.5497969574709671 1106 76-77 0.3349839159530177 1106 78-79 0.43907280662427084 1106 80-81 0.3942253512376439 1106 82-83 0.5480302619353168 1106 84-85 0.5425346357909326 1106 86-87 0.49634135860990014 1106 88-89 0.4484019873243561 1106 90-91 0.41676929862391887 1106 92-93 0.3917002902508244 1106 94-95 0.375644067511395 1106 96-97 0.3970588232978258 1106 98-99 0.464335227552791 1106 100-101 0.4217700411598386 1107 1 -0.22919767763435672 1107 2 0.24623975662108677 1107 3 0.043828710209147914 1107 4 -1.30976623269866 1107 5 -1.0501853447450316 1107 6 -0.17961862189381606 1107 7 -0.8512292683071792 1107 8 -0.14172988658649643 1107 9 -1.0888023915540046 1107 10-11 0.10618147216775498 1107 12-13 0.41932683026018225 1107 14-15 0.48794401041891433 1107 16-17 0.40864833257687394 1107 18-19 -0.22849265291515763 1107 20-21 0.1677052886912307 1107 22-23 0.314038858020389 1107 24-25 -0.5595416265782021 1107 26-27 0.2759129732214163 1107 28-29 0.5228207849633932 1107 30-31 0.5101865561236636 1107 32-33 -0.330168470682942 1107 34-35 -0.0017414662697632366 1107 36-37 0.3088127669384946 1107 38-39 0.28055316895157034 1107 40-41 0.29101185128246243 1107 42-43 0.49188626728292206 1107 44-45 0.503145894298072 1107 46-47 0.4821416530782372 1107 48-49 0.3766989478419447 1107 50-51 0.4649269598278565 1107 52-53 0.49715838625691333 1107 54-55 0.48397714934874614 1107 56-57 0.45358472597633437 1107 58-59 0.3846064224077068 1107 60-61 0.3153939009668889 1107 62-63 0.4421364540508037 1107 64-65 0.5429228492833715 1107 66-67 0.6210413314660599 1107 68-69 0.6022345395377755 1107 70-71 0.5396731266954831 1107 72-73 0.4648024923369434 1107 74-75 0.3057179583190326 1107 76-77 0.25687143113355404 1107 78-79 0.3764613292795538 1107 80-81 0.30814117485115844 1107 82-83 0.45860071648471035 1107 84-85 0.4815530125858807 1107 86-87 0.5192176368934867 1107 88-89 0.5260943127501996 1107 90-91 0.5286021333308 1107 92-93 0.37125547412417603 1107 94-95 0.44251066025354646 1107 96-97 0.43081297252883743 1107 98-99 0.5197491001559449 1107 100-101 0.6071496975475554 1108 1 -1.200266089404888 1108 2 0.00617324878827219 1108 3 -2.4901890563235085 1108 4 -3.4310920463383567 1108 5 -2.857308592852533 1108 6 -1.0760040306149037 1108 7 -0.2239251308698229 1108 8 -0.18624025236142927 1108 9 -1.2891855186993055 1108 10-11 -0.8635613652018463 1108 12-13 0.24779177858929557 1108 14-15 0.553602478808493 1108 16-17 0.5272526174510119 1108 18-19 0.374557774593562 1108 20-21 0.5057106234551441 1108 22-23 0.3381871875375282 1108 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0.5693001495113243 2310 78-79 0.696182009309112 2310 80-81 0.5170113742427134 2310 82-83 0.57413784049157 2310 84-85 0.6384056952595252 2310 86-87 0.6004574200550863 2310 88-89 0.618657234533849 2310 90-91 0.6125083295706233 2310 92-93 0.5437924089831725 2310 94-95 0.5402333321740187 2310 96-97 0.5812127407801917 2310 98-99 0.6893702312712868 2310 100-101 0.7459246399742341 2311 1 -0.22537967635186362 2311 2 0.015787232579882016 2311 3 -0.06017482474566549 2311 4 1.9487179494375937 2311 5 -2.7659728789673004 2311 6 -0.7361273208144468 2311 7 -0.19081858965456888 2311 8 -0.08947540429809919 2311 9 -0.06457726641995265 2311 10-11 0.17453383786862986 2311 12-13 0.32821440448243067 2311 14-15 0.40118868591878254 2311 16-17 0.24956277111803615 2311 18-19 0.21768027993625338 2311 20-21 0.1674631536523279 2311 22-23 0.16485327008147976 2311 24-25 -1.089533599930391 2311 26-27 -0.40450437686367735 2311 28-29 -0.41753467941808253 2311 30-31 0.13683563742728921 2311 32-33 0.2666008645495026 2311 34-35 0.25442667130171515 2311 36-37 0.3320853780473385 2311 38-39 0.2556342630170434 2311 40-41 0.21315726644363764 2311 42-43 0.3213880233695008 2311 44-45 0.315924753507133 2311 46-47 0.40947822414010204 2311 48-49 0.32151213048193483 2311 50-51 0.1490102961862263 2311 52-53 0.25864459795005956 2311 54-55 0.17010084835165173 2311 56-57 0.11880507526851858 2311 58-59 0.20979339142451892 2311 60-61 0.1656849871512378 2311 62-63 0.08413603675558079 2311 64-65 0.23945008840271242 2311 66-67 0.30538291669507345 2311 68-69 0.28290895090168533 2311 70-71 0.3703852996050827 2311 72-73 0.33722047763193785 2311 74-75 0.29435633062992395 2311 76-77 0.19446477148931507 2311 78-79 0.30691395542703503 2311 80-81 0.29924639341058423 2311 82-83 0.2481186108233615 2311 84-85 0.23856151469399478 2311 86-87 0.2751449911806674 2311 88-89 0.2885848517842753 2311 90-91 0.15311736662368958 2311 92-93 0.1492930635231815 2311 94-95 0.08662721533337248 2311 96-97 0.10121126912708078 2311 98-99 0.3040909003050629 2311 100-101 0.435116718198465 2312 1 -2.240701050160318 2312 2 -3.5103762068420714 2312 3 -4.562196658392839 2312 4 -4.682608166297154 2312 5 -3.720138144923464 2312 6 -2.5239506045000724 2312 7 -0.950796781889764 2312 8 -0.5628943052427999 2312 9 -0.23153054482379787 2312 10-11 -0.27492150813609584 2312 12-13 -0.06893241850356446 2312 14-15 0.13222850549128395 2312 16-17 0.281157634268439 2312 18-19 -0.025455047851714596 2312 20-21 0.01599848209261978 2312 22-23 0.09201783435368327 2312 24-25 0.08372233350090852 2312 26-27 0.08877064620497066 2312 28-29 0.2194519003034543 2312 30-31 0.2569798582973988 2312 32-33 0.3473939961212835 2312 34-35 0.23050398946926975 2312 36-37 0.1071231143278979 2312 38-39 0.17173957374626525 2312 40-41 0.32241455011991604 2312 42-43 -1.3970523719490373 2312 44-45 0.016227869768812297 2312 46-47 0.4136406324182431 2312 48-49 0.40759418963842364 2312 50-51 0.26641404754453646 2312 52-53 0.23804388604322213 2312 54-55 -0.06382791602986515 2312 56-57 0.14979214377093086 2312 58-59 0.25353040768633406 2312 60-61 -0.5728755241880492 2312 62-63 0.12052585024534324 2312 64-65 0.2893536383022308 2312 66-67 0.37517237774266476 2312 68-69 0.33665739777993053 2312 70-71 0.3360887086283455 2312 72-73 0.4433226701377393 2312 74-75 0.520757575118715 2312 76-77 0.320827114560327 2312 78-79 0.41123803805638914 2312 80-81 0.35841144047609674 2312 82-83 0.18660995386287738 2312 84-85 0.2870572452071052 2312 86-87 0.5004666975575383 2312 88-89 0.4860188979538229 2312 90-91 0.5246378710349902 2312 92-93 0.41980772591167437 2312 94-95 0.3538675569971552 2312 96-97 0.44062460429022465 2312 98-99 0.6543892872468717 2312 100-101 0.7522921261904223 2313 1 -0.28378823941865505 2313 2 -1.4848470912710887 2313 3 -0.04169182499101254 2313 4 1.7119623472586767 2313 5 1.2666439129931675 2313 6 0.1557072383908107 2313 7 -0.027254977540792424 2313 8 -0.4201177938772318 2313 9 -1.2683891777398841 2313 10-11 -1.746898554050155 2313 12-13 -0.4133615731581344 2313 14-15 -0.40467922755714625 2313 16-17 -0.37624550422032144 2313 18-19 -1.3022079791525343 2313 20-21 -0.7066527258138819 2313 22-23 -0.34704890140938716 2313 24-25 -0.8952507529104778 2313 26-27 -1.4431046880444072 2313 28-29 -0.9296487569462002 2313 30-31 -0.7264146217738414 2313 32-33 -1.5492644015394248 2313 34-35 -0.9504272004241585 2313 36-37 -0.7613417802820521 2313 38-39 -0.30209710380067634 2313 40-41 -0.16764100892345368 2313 42-43 0.04947233064343237 2313 44-45 -1.23103440569448 2313 46-47 -0.5938206353331807 2313 48-49 -0.10898472742010767 2313 50-51 -0.5430884692768174 2313 52-53 -0.7122871090609166 2313 54-55 -1.6849961221454421 2313 56-57 -1.1603582422706111 2313 58-59 -0.3422149196822417 2313 60-61 -0.7928838718988729 2313 62-63 -0.43626566548366696 2313 64-65 -0.7656465848769969 2313 66-67 -0.4024823743998027 2313 68-69 -0.6134461673317482 2313 70-71 0.03372297182908568 2313 72-73 0.19514188613727157 2313 74-75 -0.1604076892218913 2313 76-77 -0.24897227336083816 2313 78-79 0.18531038560931634 2313 80-81 0.1411623100527173 2313 82-83 0.08578598973553397 2313 84-85 -0.34949495730379354 2313 86-87 -0.6492454305050366 2313 88-89 -0.7657320304700086 2313 90-91 -0.34722543389501226 2313 92-93 -0.2788950321001096 2313 94-95 -0.12416488814684712 2313 96-97 -0.10060383949664953 2313 98-99 0.08482278535667476 2313 100-101 0.06585572601317224 2314 1 0.35926118712049515 2314 2 0.19884594562813263 2314 3 0.5977232241518173 2314 4 1.8643469468355676 2314 5 1.4202084517841556 2314 6 0.5907145868238501 2314 7 -0.8920972058808303 2314 8 -0.9961116854523269 2314 9 -1.952957852441564 2314 10-11 -2.2439671113580246 2314 12-13 -1.3673887843906343 2314 14-15 -3.0272448729875876 2314 16-17 -2.262808825752302 2314 18-19 -0.5808312668015603 2314 20-21 -1.0015328614164716 2314 22-23 -0.8425376156527804 2314 24-25 -1.892960210322677 2314 26-27 -1.4652043866607016 2314 28-29 -1.5623168567371408 2314 30-31 -1.9014785579393347 2314 32-33 -1.118908901572425 2314 34-35 -2.6270716982743636 2314 36-37 -2.5861399206721885 2314 38-39 -1.3655621745486854 2314 40-41 -1.2240513875589016 2314 42-43 -1.8907881719993114 2314 44-45 -1.268902482873159 2314 46-47 -1.26571515633821 2314 48-49 -1.4355449542932632 2314 50-51 -1.3440891041215153 2314 52-53 -2.2210333681486745 2314 54-55 -3.0938793245050036 2314 56-57 -3.858157758563493 2314 58-59 -1.6431172415974942 2314 60-61 -2.1659259781011784 2314 62-63 -5.073540018068655 2314 64-65 -2.572863540464244 2314 66-67 -1.4321714873768983 2314 68-69 -1.6950114348585004 2314 70-71 -1.0000159701453413 2314 72-73 -1.7224710382327544 2314 74-75 -2.7180257823806926 2314 76-77 -2.117520034006308 2314 78-79 -2.996715379847341 2314 80-81 -2.5998501089501573 2314 82-83 -1.7531485254830983 2314 84-85 -1.355907930584248 2314 86-87 -1.4365587606268022 2314 88-89 -1.3183743421520866 2314 90-91 -1.7230693327789623 2314 92-93 -1.657821750690374 2314 94-95 -1.84686305234138 2314 96-97 -1.8222267311746556 2314 98-99 -1.496800673522234 2314 100-101 -2.3364164162246546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11080.0 3 1443.0 4 1889.0 5 4604.0 6 13347.0 7 35953.0 8 75151.0 9 109937.0 10 138214.0 11 148481.0 12 153973.0 13 160729.0 14 173810.0 15 193915.0 16 216971.0 17 238385.0 18 257678.0 19 280161.0 20 295896.0 21 286636.0 22 316939.0 23 368664.0 24 448489.0 25 561725.0 26 712770.0 27 893101.0 28 1109285.0 29 1364377.0 30 1648587.0 31 1972205.0 32 2349596.0 33 2807811.0 34 3422556.0 35 4288001.0 36 5666677.0 37 7383787.0 38 7131942.0 39 1480763.0 40 1934.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.222826852964864 22.286498864664956 15.787910731635948 25.702763550734232 2 21.12542277673506 20.730247630821687 40.54668630911101 17.597643283332243 3 22.958282873773616 26.320873406719876 29.50467081391673 21.21617290558978 4 25.03953065041114 30.599128943156163 25.120490261390678 19.240850145042025 5 22.39889496476977 33.96567455210989 27.82392601413849 15.811504468981855 6 16.737504382326605 42.06915410899055 24.750548189413752 16.442793319269082 7 14.034224670708628 19.213911082951608 47.79735308542972 18.954511160910045 8 16.34725849223311 24.315972906895738 38.56095372781 20.775814873061158 9 18.580217346279156 24.501767290506812 33.5685747280689 23.349440635145132 10-11 19.50179532541271 35.023608814876354 25.634985268405973 19.83961059130496 12-13 18.524295199255633 27.763484137015617 34.6432200832992 19.069000580429556 14-15 19.45879467483913 27.485800245349495 32.41589833055948 20.639506749251893 16-17 21.855566745392156 27.116248776989956 31.196995496772033 19.831188980845862 18-19 20.827191513204806 28.595465981011337 30.795413198345763 19.781929307438098 20-21 21.39020411594364 29.820868507688264 29.31190442142995 19.477022954938146 22-23 21.591127110458665 29.41369101993365 30.756079385524497 18.23910248408318 24-25 20.75148430216448 30.048246535700795 30.68493497246073 18.51533418967399 26-27 21.513968509003075 29.09106000775991 29.772549460671417 19.622422022565598 28-29 21.624608333076644 29.417253493210666 29.285507734174764 19.672630439537926 30-31 20.10445300392045 29.633839599294564 30.454034177876927 19.807673218908057 32-33 19.939065520759204 29.585182347158018 29.917556904436665 20.558195227646113 34-35 19.963005023988213 30.127937979718457 28.97014909622219 20.93890790007114 36-37 20.19373580880304 29.18706873223015 29.89264042271499 20.726555036251817 38-39 20.74190954961249 28.68973285700606 29.952358960171455 20.615998633209994 40-41 21.202052425850802 28.61661713620934 29.69266643317295 20.48866400476691 42-43 21.066054710850995 29.24907855187463 30.00992220483223 19.674944532442147 44-45 21.103584880094704 29.48571608638142 30.107196103050498 19.303502930473375 46-47 21.12940921805188 29.0485926290127 29.95053615692488 19.87146199601054 48-49 21.687788348642652 29.26581770015144 29.091491686677294 19.954902264528616 50-51 20.668869585285933 29.398076784917905 29.723351420316256 20.20970220947991 52-53 20.183786718235798 29.239768510719845 30.123921443276274 20.452523327768084 54-55 19.741223267990748 29.694118609168168 29.936608168356305 20.62804995448478 56-57 20.102293611979675 29.389142803742736 29.84427005178431 20.664293532493282 58-59 20.453499713773287 29.23661530976344 29.58855112916027 20.721333847302997 60-61 20.942531327614475 29.646004744272737 29.186061782776303 20.225402145336485 62-63 20.470335471715163 29.728442202265825 29.643871540392635 20.157350785626377 64-65 20.964730907992056 28.969702381229183 29.800163216276754 20.265403494502003 66-67 20.87457229041828 29.632920515403384 29.188570417318815 20.303936776859523 68-69 21.03555081968061 29.433849951188424 29.434379899642998 20.096219329487973 70-71 21.128266277861353 29.378411736588404 29.221143605951877 20.272178379598362 72-73 20.005728549465303 30.676029447962538 28.727467638388855 20.590774364183307 74-75 20.008725428859528 29.392513902182632 29.751726530502854 20.847034138454987 76-77 20.611455139411216 29.52483782204931 28.888591963213138 20.975115075326332 78-79 20.056148559116302 30.562129643244475 28.585903121870356 20.795818675768867 80-81 19.927808535129916 30.015622805682163 29.674550709611054 20.382017949576863 82-83 20.337744125096464 30.183032026109082 29.22443264526114 20.25479120353332 84-85 20.658373500170534 30.032783717521745 29.20191860381133 20.106924178496396 86-87 21.12508558099238 30.075467789943776 28.460972840699704 20.338473788364137 88-89 21.40524464706291 29.95079992923717 28.606953699899474 20.037001723800444 90-91 20.995687373942822 30.09182191877557 28.739737613044884 20.17275309423672 92-93 20.9957606192064 30.29225897381698 28.430234790974684 20.281745616001935 94-95 20.6218079772889 30.2355636640806 28.721429319823915 20.421199038806584 96-97 20.702882180441495 30.474692332453344 28.10933126212232 20.713094224982836 98-99 20.432276056059496 30.6486308748448 28.158990850059784 20.760102219035918 100-101 20.976558637853902 30.77606579897461 27.637706687473667 20.609668875697817 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19829.0 1 14952.0 2 9465.5 3 9666.5 4 14189.0 5 19397.5 6 20170.0 7 18874.0 8 19586.0 9 21132.5 10 23947.0 11 30206.0 12 33988.5 13 40296.0 14 44079.5 15 44365.0 16 52976.5 17 66042.0 18 82846.5 19 96498.5 20 115400.0 21 136400.0 22 155367.0 23 183350.5 24 221652.5 25 276264.0 26 336599.0 27 408039.0 28 501821.0 29 605113.0 30 802626.5 31 990299.5 32 1418359.0 33 1803561.5 34 1826361.0 35 2039017.0 36 2276957.0 37 2250431.0 38 2057480.0 39 1863661.5 40 1748289.5 41 1675356.0 42 1616181.5 43 1637916.5 44 1703400.0 45 1727820.5 46 1724674.0 47 1678102.5 48 1663924.5 49 1601493.5 50 1442248.0 51 1277780.5 52 1093402.0 53 916290.0 54 769337.0 55 657104.0 56 559954.5 57 470795.5 58 401471.0 59 339293.0 60 274012.0 61 217278.0 62 168515.5 63 125165.0 64 89913.0 65 62641.5 66 42667.5 67 30314.5 68 21732.0 69 14620.5 70 9932.0 71 6436.5 72 4426.5 73 3390.5 74 2612.5 75 1887.5 76 1308.0 77 957.5 78 766.5 79 605.0 80 465.0 81 357.5 82 274.0 83 226.0 84 190.5 85 156.5 86 127.0 87 93.0 88 68.0 89 51.5 90 33.0 91 19.0 92 12.0 93 7.0 94 5.5 95 5.0 96 2.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16743259028277632 2 1.7120553219860304E-4 3 5.863789477802155E-4 4 0.0036252771443054197 5 5.6497825625539E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.03104598319506418 16-17 0.0011470770657306404 18-19 0.0 20-21 0.0 22-23 2.140069152482538E-6 24-25 0.013225627362342085 26-27 0.06648766842932749 28-29 0.057583910720423896 30-31 0.04842976492067984 32-33 0.027168177890765824 34-35 0.024373247577623627 36-37 0.02024398414790857 38-39 0.022904090104444365 40-41 0.02049009210044406 42-43 0.026841817345012235 44-45 0.03128032076726102 46-47 0.028548522494117053 48-49 0.04188864355611696 50-51 0.14973207832259325 52-53 0.2960785672459591 54-55 0.29672486813000887 56-57 0.38224203146321106 58-59 0.3768158861270916 60-61 0.3716144480519828 62-63 0.35610322683478934 64-65 0.36690094574364 66-67 0.3304009963134741 68-69 0.25500100989863306 70-71 0.24889432257202415 72-73 0.2542498456261117 74-75 0.26418939680481685 76-77 0.24925599425879366 78-79 0.21571469043193486 80-81 0.22452428509812924 82-83 0.2084395253480705 84-85 0.1462180847742169 86-87 0.04957684198641048 88-89 0.017156934395452506 90-91 0.007230223631662255 92-93 0.031048123264216663 94-95 0.018098564822544825 96-97 0.03833398869384346 98-99 0.05016536100334318 100-101 0.04303251051811888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.6727462E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.227207349456506 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86904059838265 27.48523465161942 2 10.834943718756477 7.8503950543816465 3 4.306727749101012 4.680621574930213 4 2.313822164620604 3.3529326130991564 5 1.395289281543582 2.527371705747677 6 0.8972455098918934 1.9502819478133475 7 0.688206357508508 1.745225608887244 8 0.5067513689183037 1.4686549533139457 9 0.39975510415179943 1.303380994239974 >10 2.4143538211202507 16.44491229978112 >50 0.21708401347709214 5.423519466863644 >100 0.13730526574564375 9.475159226155782 >500 0.01081022776180753 2.689460289583875 >1k 0.007104885537687865 5.08206179826863 >5k 7.950241012991222E-4 2.0092647463314965 >10k+ 7.649093813281009E-4 6.511523068982954 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 175103 0.37473252880714986 No Hit ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT 152754 0.32690412331831764 No Hit GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT 99832 0.21364738363063673 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 72735 0.1556579298058174 No Hit GTCGTATTACAATCCTATAGTGAGTCGTATTACAATCCTATAGTGAGTCG 69898 0.14958655362022444 No Hit ATACGACTCACTATAGGATTGTAATACGACTCACTATAGGATTGTAATAC 59271 0.1268440387367925 No Hit ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA 53885 0.11531762628152156 No Hit GTCGTATTACAATTCCTATAGTGAGTCGTATTACAATTCCTATAGTGAGT 50792 0.10869839239289307 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 49790 0.10655404310210556 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.010563381336653807 0.0 0.0 4.280138304965076E-6 2.140069152482538E-6 2 0.010601902581398493 6.420207457447614E-6 0.0 1.4980484067377766E-5 2.140069152482538E-6 3 0.010653264241058074 6.420207457447614E-6 0.0 1.4980484067377766E-5 6.420207457447614E-6 4 0.010826609842409159 6.420207457447614E-6 0.0 2.5680829829790456E-5 8.560276609930152E-6 5 0.010884391709526188 6.420207457447614E-6 0.0 2.5680829829790456E-5 8.560276609930152E-6 6 0.010972134544777972 6.420207457447614E-6 0.0 2.5680829829790456E-5 8.560276609930152E-6 7 0.011029916411895001 1.070034576241269E-5 0.0 3.210103728723807E-5 8.560276609930152E-6 8 0.011149760284434023 1.2840414914895228E-5 0.0 3.638117559220315E-5 1.070034576241269E-5 9 0.011314545609175178 1.2840414914895228E-5 0.0 3.8521244744685685E-5 1.070034576241269E-5 10-11 0.011735069197637997 1.2840414914895228E-5 0.0 4.9221590507098374E-5 1.070034576241269E-5 12-13 0.012483023366430646 1.4980484067377767E-5 0.0 5.457176338830472E-5 1.2840414914895228E-5 14-15 0.013451404657928993 1.7120553219860305E-5 0.0 6.527210915071742E-5 1.4980484067377766E-5 16-17 0.014701205042978796 1.7120553219860305E-5 0.0 7.27623511844063E-5 1.4980484067377766E-5 18-19 0.01634156804835666 1.7120553219860305E-5 0.0 7.59724549131301E-5 1.6050518643619036E-5 20-21 0.01829117104626825 1.7120553219860305E-5 0.0 8.025259321809517E-5 2.033065694858411E-5 22-23 0.020663437701795146 1.7120553219860305E-5 0.0 9.095293898050787E-5 2.140069152482538E-5 24-25 0.02305175487596566 1.8190587796101574E-5 0.0 9.630311186171422E-5 2.140069152482538E-5 26-27 0.025664779311146836 1.9260622372342843E-5 0.0 1.0272331931916182E-4 2.4610795253549187E-5 28-29 0.028843852037159647 2.140069152482538E-5 0.0 1.0807349220036817E-4 2.5680829829790456E-5 30-31 0.03224549195503064 2.140069152482538E-5 0.0 1.1342366508157452E-4 2.6750864406031725E-5 32-33 0.03606765546136445 2.247072610106665E-5 0.0 1.1449369965781578E-4 2.7820898982272993E-5 34-35 0.04035421397378698 2.8890933558514262E-5 0.0 1.2198394169150467E-4 2.8890933558514262E-5 36-37 0.045060226040096074 3.424110643972061E-5 0.0 1.241240108439872E-4 2.996096813475553E-5 38-39 0.050280924737577226 3.424110643972061E-5 0.0 1.284041491489523E-4 3.424110643972061E-5 40-41 0.05621105635910634 3.424110643972061E-5 0.0 1.3268428745391735E-4 3.638117559220315E-5 42-43 0.06269867599485715 3.8521244744685685E-5 0.0 1.4659473694505386E-4 3.8521244744685685E-5 44-45 0.07064047261971985 4.066131389716822E-5 0.0 1.5301494440250147E-4 4.280138304965076E-5 46-47 0.08013809951843737 4.066131389716822E-5 0.0 1.5943515185994908E-4 4.280138304965076E-5 48-49 0.09071967144288727 4.066131389716822E-5 0.0 1.7869577423229192E-4 4.280138304965076E-5 50-51 0.10306038021067782 4.066131389716822E-5 0.0 1.8404594711349828E-4 4.280138304965076E-5 52-53 0.11603882958590818 4.280138304965076E-5 0.0 1.8832608541846334E-4 4.280138304965076E-5 54-55 0.129236636049268 4.280138304965076E-5 0.0 1.8832608541846334E-4 4.3871417625892026E-5 56-57 0.1419390165038281 4.280138304965076E-5 0.0 1.9153618914718716E-4 4.49414522021333E-5 58-59 0.15448410187568073 4.49414522021333E-5 0.0 1.968863620283935E-4 4.49414522021333E-5 60-61 0.16800933891937037 4.7081521354615836E-5 0.0 1.968863620283935E-4 4.49414522021333E-5 62-63 0.1831492581386081 4.81515559308571E-5 0.0 1.9795639660463477E-4 4.81515559308571E-5 64-65 0.20084120982218123 5.0291625083339646E-5 0.0 1.9902643118087604E-4 4.9221590507098374E-5 66-67 0.2223221539402247 5.136165965958091E-5 0.0 2.0116650033335858E-4 5.0291625083339646E-5 68-69 0.24801154404662507 5.350172881206345E-5 0.0 2.0116650033335858E-4 5.136165965958091E-5 70-71 0.28000129773793403 5.7781867117028525E-5 0.0 2.0223653490959986E-4 5.136165965958091E-5 72-73 0.31970921082767134 6.20620054219936E-5 0.0 2.075867077908062E-4 5.136165965958091E-5 74-75 0.37289955957804855 6.313203999823487E-5 0.0 2.1186684609577125E-4 5.136165965958091E-5 76-77 0.4400645598941368 6.420207457447614E-5 0.0 2.1828705355321889E-4 5.136165965958091E-5 78-79 0.5263681986408764 6.527210915071742E-5 0.0 2.247072610106665E-4 5.136165965958091E-5 80-81 0.6395553860811015 6.848221287944122E-5 0.0 2.3861771050180298E-4 5.136165965958091E-5 82-83 0.7804575390805518 7.062228203192375E-5 0.0 2.5038809084045694E-4 5.136165965958091E-5 84-85 0.9649850017533588 7.169231660816502E-5 0.0 2.589483674503871E-4 5.136165965958091E-5 86-87 1.1937369934622173 7.490242033688883E-5 0.0 2.653685749078347E-4 5.136165965958091E-5 88-89 1.4722359626551085 7.490242033688883E-5 0.0 2.7178878236528233E-4 5.136165965958091E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGACG 3525 0.0 41.16092 2 ACGACGG 10420 0.0 27.212036 1 CGACGGC 10735 0.0 24.734821 2 GCGCCTA 5435 0.0 23.119003 2 GACGGCC 13335 0.0 20.374334 3 GTCGAAT 10135 0.0 19.30758 1 ACGGCCA 15320 0.0 18.105755 4 GGGCGCT 9195 0.0 17.820578 1 TATGACT 18310 0.0 16.366337 2 GTCTTAT 18540 0.0 15.831904 1 ATATGAC 18775 0.0 15.583147 1 CGGCCAG 21045 0.0 15.50466 1 GCGTGTT 45135 0.0 15.489905 1 AAACGAC 12685 0.0 15.276508 1 TCGAATT 12905 0.0 15.247999 2 ATGACTC 21465 0.0 14.976855 3 TATGCCG 10225 0.0 14.891551 46-47 TGCCGTC 10325 0.0 14.873285 48-49 CGTGTTT 44455 0.0 14.729765 2 TGACTCA 24100 0.0 14.539413 4 >>END_MODULE