FastQCFastQC Report
Fri 27 May 2016
SRR1233102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74709628
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC11481491.5368153084633214No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC6381030.8541107981423759No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA4411300.5904593715819332No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT4144450.5547410837061054No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT4122610.5518177657101974No Hit
TTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACAA4115410.550854034502755No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC3829540.5125898900206008No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA3239510.4336134560862758No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT2635670.3527885321554539No Hit
CAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTT2394260.32047542787925537No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT2329370.3117898003721823No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA2260990.30263703093261285No Hit
AGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAAAAGAAGAG1954360.2615941281356668No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT1820390.24366203509941184No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT1815350.24298742325420225No Hit
TTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTT1788770.23942964888006135No Hit
AAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTT1754260.23481043166216808No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT1749610.23418802192402832No Hit
CGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAA1701380.22773236134973124No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA1591870.2130742773876481No Hit
GAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCC1489430.19936252393065054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1448400.1938705945637957No Hit
AAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGGCTAAGTCCG1439600.19269270086581075No Hit
TTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACA1423970.19060060103632157No Hit
TGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAAC1375390.1840980924172183No Hit
CGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGA1314860.1759960576968741No Hit
TATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGT1298960.17386781794710582No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT1290030.17267252354676427No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC1264860.16930347986741415No Hit
TGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTT1255210.1680118123463284No Hit
AAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAA1172480.15693827306970395No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1141180.15274871934846201No Hit
GCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAA1140100.1526041596673457No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1135560.15199647360043073No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1124130.15046655030861616No Hit
GAAAAATTGAAAGCCAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAG1077340.14420363597580757No Hit
ATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTT1075820.14400018160979197No Hit
GGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCA1060800.1419897312298222No Hit
TATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTT1048540.14034871114603864No Hit
GGTGACTCCAAAATTTTCAGATTCCAAAACTCCAAATCTGCTTCTTTGTT1027340.13751105814634762No Hit
TAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTT1005350.13456766241695112No Hit
ATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTA998090.13359590011611355No Hit
AAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAA987860.13222659869220604No Hit
ATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTT976710.13073415383623646No Hit
CAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGA974850.13048518994098057No Hit
CCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGG937730.1255166201603895No Hit
CAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGT925950.12393984882376874No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG924630.123763164769071No Hit
ACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATT903210.12089606442693035No Hit
CCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAA887430.1187838868639528No Hit
TTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGAAAAG865430.11583915261899043No Hit
CAAAGACCAATCACTGGTGCTTCTTTGGACTTGATCAAGGAAAGAAGATC861540.11531847006385842No Hit
CTTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGAAAA859030.11498250265681954No Hit
CACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTT851200.11393444496872612No Hit
AAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTTT849960.1137684690385555No Hit
TTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATT840730.1125330191712372No Hit
CCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTT813590.10890028792540633No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC807700.10811190225709597No Hit
CAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAA806670.1079740351538091No Hit
TTTCTATTGTATCACGGTCTTTTATATATTATATTATTTGATACTTAACA800880.10719903464115764No Hit
GAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAAAAGAAGAGA794830.10638923272379297No Hit
GCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATA790990.10587524274649045No Hit
TTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCACTTCCC782750.10477230592019546No Hit
AAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTT770990.10319821161470646No Hit
AAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCAC766330.1025744633610008No Hit
GCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCA757480.10138987708518639No Hit
GCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAACC750340.1004341769711395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT3273750.038.2992671
TTTTATA3538950.034.4842222
TTATATA3579900.033.39894
TATTATA3786100.031.4259179
TTTATAT3852300.031.3416353
TATATAT4213400.028.373355
CGGCGCT220550.022.7883051
CGTACTT591050.021.66356794-95
CCGTACT688200.020.95351294-95
CGCTAAG231050.020.3505044
TTACGCG305100.018.2527546
TCCGTAC707350.018.03462692-93
CGGGTAT44050.017.5200651
ATCCGTA847100.017.31435492-93
GGCGCTA278750.017.2441922
CGTGATC846850.016.89915888-89
ACGCGCA333700.016.8649448
GCGCTAA287450.016.573123
TACGCGC340150.016.5539057
TGATCCG873050.016.4599790-91
Produced by FastQC (version 0.11.5)