FastQCFastQC Report
Fri 27 May 2016
SRR1233102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74709628
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC10890051.4576501438342058No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC7018900.9394906905439282No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT4920970.6586794944287502No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA4379270.5861721062243811No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA3889810.5206571233362318No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT3789600.5072438588504282No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC3552080.4754514371293617No Hit
TTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACAA3523340.47160454339298813No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT2691060.3602025698749296No Hit
CAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTT2438190.3263555267602189No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT2309960.30919174165878593No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA2146730.287343152076731No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT2122230.2840637889402956No Hit
ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2098610.2809022151736587No Hit
CGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAA1907240.25528704278918374No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT1879400.2515606154537405No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT1817190.24323371011832637No Hit
TTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTT1791710.2398231724564336No Hit
AGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAAAAGAAGAG1729880.2315471307125234No Hit
AAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTT1691100.2263563673479943No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA1668290.22330321334219466No Hit
GAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCC1627060.217784513664022No Hit
CGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGA1567420.20980160682904217No Hit
AAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGGCTAAGTCCG1514510.20271952096990764No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC1487840.1991496999556737No Hit
TGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAAC1485090.19878160817505341No Hit
GGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCA1335010.17869316656214645No Hit
GCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAA1292110.17295093478446982No Hit
TATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGT1270980.17012265139374003No Hit
TTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACA1218170.16305395069026446No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT1194390.1598709606745733No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1154830.15457579309590458No Hit
AAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAA1144500.15319310651633816No Hit
CAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGA1141380.15277548965977988No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1129680.15120942644768623No Hit
TAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTT1112570.14891922631444504No Hit
CCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGG1079490.14449141682247435No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1079230.14445661541776114No Hit
ATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTT1076940.1441500953531719No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1059590.1418277708463493No Hit
GAAAAATTGAAAGCCAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAG1055550.1412870105577289No Hit
TATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTT1046530.14007966951729434No Hit
AAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAA1029410.13778813086848726No Hit
CCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAA1017070.13613640266017654No Hit
TTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTT1009290.1350950375499126No Hit
ATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTA978370.13095634742017456No Hit
ACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATT968780.12967271099248412No Hit
ATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTT947480.1268216728371342No Hit
TGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTT930770.12458501332652867No Hit
CCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTT913490.12227205842866731No Hit
GGTGACTCCAAAATTTTCAGATTCCAAAACTCCAAATCTGCTTCTTTGTT906640.1213551752660313No Hit
CAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTT888070.1188695518601699No Hit
CAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGT885460.11852019929747208No Hit
TTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATT878530.11759260801030892No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG877200.1174145854400453No Hit
GCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCA876560.1173289204438282No Hit
TGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCC872610.11680020679530087No Hit
TTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCACTTCCC857120.11472684618373417No Hit
AAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTT856610.11465858188987368No Hit
CAAAGACCAATCACTGGTGCTTCTTTGGACTTGATCAAGGAAAGAAGATC845270.11314070523815217No Hit
GCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAACC843800.11294394344996606No Hit
TTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGAAAAG841010.11257049760708218No Hit
AAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCAC833780.11160275085294227No Hit
CACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTT832450.11142472828267863No Hit
CTTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGAAAA824840.11040611793703484No Hit
GGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTA820130.10977567710549971No Hit
AAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTTT798090.10682558879827377No Hit
CAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAA785450.10513370512298628No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC774620.10368409276512527No Hit
GCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATA766020.10253296937845816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT3118600.037.735741
TTTTATA3441250.033.2554932
TTATATA3375900.033.178064
TATTATA3580500.031.2231169
TTTATAT3685150.030.6692283
TATATAT4003400.028.0722375
CGGCGCT246900.026.0224021
CGTACTT489500.025.6067994-95
CGACGGC134750.025.568251
CGGCCAG285100.025.402681
CCGTACT606600.024.6436694-95
GACGGCC151850.024.4593332
CGCTAAG238700.022.2032454
TCCGTAC633550.019.66099292-93
ATCCGTA775250.019.56132992-93
CGTGATC787200.018.91495188-89
ACGGCCA197050.018.8239353
CGGGTAT42800.018.76441
TGATCCG805950.018.53970790-91
GGCGCTA293050.018.249432
Produced by FastQC (version 0.11.5)