FastQCFastQC Report
Fri 27 May 2016
SRR1233101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57353778
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG621640.10838693137180955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATT1938200.017.1247371
GGAAAAC1770200.016.4132731
CTGGAAT1183200.014.7328551
GGCGAAT44400.014.3821311
TTTATTC1106500.014.1950572
CGGCAGT77300.014.0558371
TTAATCG45450.014.0498693
GTTTATT1253750.013.2652631
CGGGTAT32100.013.0640851
CTTTAAT1155450.012.9379281
GTTTTAT1023750.012.8288191
CGGCTAT31900.012.6978321
AATCGAC43000.012.634865
AATCGGC39150.012.2948635
CGGGGAT62150.012.2681821
CGCCGGA29800.012.2615446-47
CTTTTAT1067050.011.8527061
ATCGACC28450.011.7254746
GGAATCG64050.011.681053
CGGCAAC90700.011.4538251
Produced by FastQC (version 0.11.5)