##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233101_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57353778 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.268802920009907 31.0 31.0 34.0 28.0 34.0 2 31.586704244661963 33.0 31.0 34.0 28.0 34.0 3 31.669856447817615 34.0 31.0 34.0 30.0 34.0 4 35.124622426791134 37.0 35.0 37.0 32.0 37.0 5 35.0759986029168 37.0 35.0 37.0 32.0 37.0 6 35.05044788854188 37.0 35.0 37.0 32.0 37.0 7 35.015584256716274 37.0 35.0 37.0 32.0 37.0 8 35.06166247670729 37.0 35.0 37.0 32.0 37.0 9 36.689681140098564 39.0 37.0 39.0 33.0 39.0 10-11 36.707038218127494 39.0 37.0 39.0 33.0 39.0 12-13 36.622937742654024 39.0 37.0 39.0 32.0 39.0 14-15 37.77572633488939 40.0 38.0 41.0 32.0 41.0 16-17 37.70229557501862 40.0 38.0 41.0 32.0 41.0 18-19 37.72887020624866 40.0 38.0 41.0 32.0 41.0 20-21 37.758533727281225 40.0 38.0 41.0 32.0 41.0 22-23 37.692075045169645 40.0 38.0 41.0 32.0 41.0 24-25 36.94218587483461 39.5 37.0 41.0 30.0 41.0 26-27 35.81534360299682 39.0 34.5 41.0 27.0 41.0 28-29 35.464929930160835 39.0 34.0 41.0 25.5 41.0 30-31 35.285259447076 39.0 34.0 41.0 25.0 41.0 32-33 35.183081583570655 39.0 34.0 41.0 25.0 41.0 34-35 34.93587271617922 38.0 34.0 40.5 24.0 41.0 36-37 34.8526740557527 38.0 34.0 40.0 24.0 41.0 38-39 34.77857462153583 38.0 34.0 40.0 24.0 41.0 40-41 34.77289156958413 38.0 33.5 40.0 24.0 41.0 42-43 34.84404087556359 38.0 34.0 41.0 24.0 41.0 44-45 34.77560412846735 38.0 33.5 41.0 24.0 41.0 46-47 34.690564778487655 38.0 33.0 41.0 24.0 41.0 48-49 34.58784417828586 38.0 33.0 41.0 23.5 41.0 50-51 34.47070239209002 38.0 33.0 40.5 23.0 41.0 52-53 34.35060109728081 38.0 33.0 40.0 23.0 41.0 54-55 34.20334978630353 38.0 33.0 40.0 22.5 41.0 56-57 33.99745222537912 38.0 33.0 40.0 21.5 41.0 58-59 33.78799198720614 38.0 33.0 40.0 20.5 41.0 60-61 33.56875621166578 38.0 32.0 40.0 19.5 41.0 62-63 33.33217879735839 37.5 32.0 40.0 19.0 41.0 64-65 33.03233174107554 37.0 31.5 40.0 17.5 41.0 66-67 32.68450908325516 37.0 31.0 40.0 13.5 41.0 68-69 32.390110534653886 36.0 31.0 39.0 13.0 41.0 70-71 32.0742012583722 36.0 31.0 39.0 13.0 41.0 72-73 31.80483742500799 35.0 31.0 39.0 11.5 40.5 74-75 31.43753854366839 35.0 30.0 38.5 8.0 40.0 76-77 31.08101946658161 35.0 30.0 37.5 7.5 39.5 78-79 30.729547894822204 35.0 30.0 37.0 7.0 39.0 80-81 30.38382141975024 35.0 30.0 36.5 4.5 39.0 82-83 30.05082464663444 34.5 29.5 36.0 2.0 38.5 84-85 29.711514252818706 34.0 29.0 36.0 2.0 37.0 86-87 29.360602164690874 34.0 29.0 35.0 2.0 37.0 88-89 29.05200782065307 34.0 29.0 35.0 2.0 36.5 90-91 28.777080622308787 34.0 29.0 35.0 2.0 36.0 92-93 28.496069622475435 34.0 29.0 35.0 2.0 36.0 94-95 28.210174532879073 34.0 29.0 35.0 2.0 36.0 96-97 27.86799416247697 34.0 28.0 35.0 2.0 35.0 98-99 27.5286668996766 34.0 27.5 35.0 2.0 35.0 100-101 26.448261394393235 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21806056771354676 1101 2 0.3380501751206886 1101 3 0.3420405666737807 1101 4 0.3508283811872559 1101 5 0.32400363934755916 1101 6 0.34363177867123085 1101 7 0.35657531541770027 1101 8 0.3492098164711308 1101 9 0.42571486949523063 1101 10-11 0.3912223436286624 1101 12-13 0.3998109952624631 1101 14-15 0.5401820780601412 1101 16-17 0.4753324621323074 1101 18-19 0.4852377601043827 1101 20-21 0.418428952228183 1101 22-23 0.4683294316251647 1101 24-25 0.4886249893680059 1101 26-27 0.4222045207389371 1101 28-29 0.30836826927522765 1101 30-31 0.2434783052312639 1101 32-33 0.25600565947929965 1101 34-35 0.32375825508289324 1101 36-37 0.23634419338728208 1101 38-39 0.27472151157274993 1101 40-41 0.34279570922750224 1101 42-43 0.3168169637101528 1101 44-45 0.256055793278648 1101 46-47 0.2820727047655609 1101 48-49 0.32899380292121805 1101 50-51 0.43035132731661463 1101 52-53 0.41876253912553096 1101 54-55 0.46247668066139624 1101 56-57 0.5025660801495917 1101 58-59 0.48751936968362486 1101 60-61 0.45232875859293387 1101 62-63 0.5476070367324652 1101 64-65 0.5172545385517466 1101 66-67 0.45400051864004354 1101 68-69 0.4857712289959437 1101 70-71 0.4975059991150914 1101 72-73 0.5110339023710573 1101 74-75 0.5066316366001722 1101 76-77 0.4859556209792295 1101 78-79 0.465216728906519 1101 80-81 0.403847125018963 1101 82-83 0.36496074136013235 1101 84-85 0.26886289978594036 1101 86-87 0.26062260434021667 1101 88-89 0.3930821417416226 1101 90-91 0.3410194000814961 1101 92-93 0.3041902914691441 1101 94-95 0.4119606078236835 1101 96-97 0.3944744448866757 1101 98-99 0.30832166044919873 1101 100-101 0.33379515970879936 1102 1 0.12686640300186625 1102 2 0.14245411068383262 1102 3 0.16163114049074423 1102 4 0.16639267946421654 1102 5 0.20598239565384802 1102 6 0.2541794476844643 1102 7 0.26057149463852625 1102 8 0.2364743772668092 1102 9 0.29721730240567723 1102 10-11 0.3098772731860251 1102 12-13 0.34652103072874496 1102 14-15 0.37252064746250824 1102 16-17 0.3211395009600295 1102 18-19 0.30726889881819375 1102 20-21 0.3283577097083423 1102 22-23 0.326434427478695 1102 24-25 0.3830428225390605 1102 26-27 0.31434904178687617 1102 28-29 0.2470695603427231 1102 30-31 0.34342827940593423 1102 32-33 0.3812583706353152 1102 34-35 0.246594426419378 1102 36-37 0.18972495213567697 1102 38-39 0.19712876698240933 1102 40-41 0.21228273187922042 1102 42-43 0.29023937021050017 1102 44-45 0.3646491595215551 1102 46-47 0.2011833040543891 1102 48-49 0.21726834112632787 1102 50-51 0.23778615604339848 1102 52-53 0.34543793663622324 1102 54-55 0.264464736718665 1102 56-57 0.24747198095397493 1102 58-59 0.2810560244211189 1102 60-61 0.2505937332959931 1102 62-63 0.19275399705048812 1102 64-65 0.17608640238235296 1102 66-67 0.2729274422820751 1102 68-69 0.32245755427457823 1102 70-71 0.29297212257849026 1102 72-73 0.15397057035031736 1102 74-75 0.12641670678756967 1102 76-77 0.24237896051954877 1102 78-79 0.2172526955985603 1102 80-81 0.1543075449357012 1102 82-83 0.09834372267488689 1102 84-85 0.16645261509640363 1102 86-87 0.21277082683629445 1102 88-89 0.1579182768236862 1102 90-91 0.16792604761195307 1102 92-93 0.14196344913338166 1102 94-95 0.1750497963941875 1102 96-97 0.17781711767550945 1102 98-99 0.14677495085176417 1102 100-101 0.08366586739951387 1103 1 0.18361769699083297 1103 2 0.1722161998350984 1103 3 0.2125620628912479 1103 4 0.13559226540350977 1103 5 0.14491693714896314 1103 6 0.17411451728839467 1103 7 0.20038239503840316 1103 8 0.16121363637560648 1103 9 0.17837519840419702 1103 10-11 0.13867350097865483 1103 12-13 0.21200490859947507 1103 14-15 0.2784598224109516 1103 16-17 0.27522834330252266 1103 18-19 0.23675155198615272 1103 20-21 0.24793986186362815 1103 22-23 0.25659432556300743 1103 24-25 0.3106964491181117 1103 26-27 0.3007644126831934 1103 28-29 0.40111109786295174 1103 30-31 0.4296524119970826 1103 32-33 0.4458976328770916 1103 34-35 0.4688304208797973 1103 36-37 0.4354749177028623 1103 38-39 0.43106929844486075 1103 40-41 0.3445544666242526 1103 42-43 0.3441001928326628 1103 44-45 0.32661186385711005 1103 46-47 0.26153230498774604 1103 48-49 0.22162170527333558 1103 50-51 0.22302718922286147 1103 52-53 0.2653382629747014 1103 54-55 0.20743881892984462 1103 56-57 0.2144668276419992 1103 58-59 0.18570796137288426 1103 60-61 0.13272809007350617 1103 62-63 0.2583526426642848 1103 64-65 0.30181195075984846 1103 66-67 0.2138793816219291 1103 68-69 0.2117152103879647 1103 70-71 0.298210438679682 1103 72-73 0.2199316469092203 1103 74-75 0.18987589929765747 1103 76-77 0.21335116008566501 1103 78-79 0.15689121286310836 1103 80-81 0.18442632472920195 1103 82-83 0.22347277399457965 1103 84-85 0.22003945849213835 1103 86-87 0.19155612775958275 1103 88-89 0.2469287156320732 1103 90-91 0.24631697819540577 1103 92-93 0.24346125534453833 1103 94-95 0.2651719263971444 1103 96-97 0.2708427118647947 1103 98-99 0.21787946476622366 1103 100-101 0.1506794435632024 1104 1 0.11284705480650103 1104 2 0.1593316807987506 1104 3 0.1770670931091658 1104 4 0.06738932104640583 1104 5 0.04512959858418952 1104 6 0.056623978326342694 1104 7 0.0622322927668435 1104 8 0.054365754742718764 1104 9 0.10933352816659436 1104 10-11 0.13122903626033633 1104 12-13 0.10229266128271064 1104 14-15 0.08241300200596413 1104 16-17 0.12210878630480693 1104 18-19 0.17800403911949303 1104 20-21 0.11225404326829391 1104 22-23 0.12927507590606524 1104 24-25 0.08570322838817646 1104 26-27 0.06229616451646791 1104 28-29 -0.05971923811890889 1104 30-31 -0.018280988525589237 1104 32-33 0.008841410256614779 1104 34-35 0.15797293948315883 1104 36-37 0.00944313551386955 1104 38-39 0.036771242357282574 1104 40-41 -0.08159749643191105 1104 42-43 -0.00785561229565701 1104 44-45 -9.372691584275117E-5 1104 46-47 -0.11284860865092128 1104 48-49 0.045054138323258996 1104 50-51 -0.060191220634806086 1104 52-53 -0.10346813842242142 1104 54-55 -0.002880321931996832 1104 56-57 0.004714224648807885 1104 58-59 -0.008295205793658056 1104 60-61 -0.10049237191177696 1104 62-63 0.04861904481435175 1104 64-65 0.10416551989595746 1104 66-67 -0.024488196592066913 1104 68-69 0.012880521364337483 1104 70-71 -0.046327499580492315 1104 72-73 0.009837686618084263 1104 74-75 0.06374303229133815 1104 76-77 0.11681753491559022 1104 78-79 0.013881972730523273 1104 80-81 0.07130382807225288 1104 82-83 -0.030047086492285047 1104 84-85 0.003962145515508553 1104 86-87 -0.035332421607247255 1104 88-89 -0.017868145267666335 1104 90-91 -0.026679609723895936 1104 92-93 -0.014328387581759472 1104 94-95 -0.006961796047225022 1104 96-97 0.030055037422613395 1104 98-99 0.0696554618631815 1104 100-101 0.052518706685095395 1105 1 0.07847501160996728 1105 2 0.11535462941925445 1105 3 0.1412503913968166 1105 4 0.07292575129540069 1105 5 0.08821717608287116 1105 6 0.15092943757812094 1105 7 0.13889439313552288 1105 8 0.14025629866637956 1105 9 0.11414543275332534 1105 10-11 0.09100086478938607 1105 12-13 0.1562677665420935 1105 14-15 0.18111458685909554 1105 16-17 0.14748775168806105 1105 18-19 0.09195385868805772 1105 20-21 0.08306416207884126 1105 22-23 0.15262485161647987 1105 24-25 0.15138257735628713 1105 26-27 0.2025409801956144 1105 28-29 0.2167858653482142 1105 30-31 0.2797673673506864 1105 32-33 0.20662638694831514 1105 34-35 0.21117585651114013 1105 36-37 0.241550917017193 1105 38-39 0.1559322185074592 1105 40-41 0.1614955784654697 1105 42-43 0.20013755801305422 1105 44-45 0.1386279971171902 1105 46-47 0.12165915905286084 1105 48-49 0.13886261633079755 1105 50-51 0.19085825271068302 1105 52-53 0.21227258434872454 1105 54-55 0.21573507213326337 1105 56-57 0.12944121712743595 1105 58-59 0.1347710382585987 1105 60-61 0.1456000331055307 1105 62-63 0.1364152550837474 1105 64-65 0.135732169230657 1105 66-67 0.13621562370109785 1105 68-69 0.0844551195315475 1105 70-71 0.03981261191403007 1105 72-73 0.0421786104356805 1105 74-75 0.10136118980265252 1105 76-77 0.028458228257385088 1105 78-79 0.046656358334168146 1105 80-81 0.10169092443174677 1105 82-83 0.11312986895025645 1105 84-85 0.07059089552079101 1105 86-87 0.07935008688620115 1105 88-89 0.016004878238646825 1105 90-91 0.05169429509002654 1105 92-93 0.033662470800450706 1105 94-95 -0.004260545910408808 1105 96-97 -0.07630910633886501 1105 98-99 -0.0111224637717946 1105 100-101 -0.10260345086435407 1106 1 -0.014934465388851237 1106 2 0.03459101924196517 1106 3 0.015882211884342468 1106 4 -0.02669992519363973 1106 5 0.025845834834960613 1106 6 0.004826528032829458 1106 7 0.06538369083434503 1106 8 0.08198490668139158 1106 9 0.09172888848362248 1106 10-11 0.02597909999526138 1106 12-13 0.012387421928082176 1106 14-15 -0.011332058823050772 1106 16-17 0.06961409780817718 1106 18-19 0.05890145484520559 1106 20-21 0.00911160036078229 1106 22-23 -0.013228068406974103 1106 24-25 -0.012058159707684979 1106 26-27 0.03303798284739656 1106 28-29 -0.046596034512553786 1106 30-31 0.05341326598637863 1106 32-33 0.00620369823349165 1106 34-35 0.04219017592085095 1106 36-37 0.05640714391901014 1106 38-39 -0.010251013053697022 1106 40-41 -0.004565816948172596 1106 42-43 -0.01749577773561839 1106 44-45 -0.05523319313800812 1106 46-47 -0.030314445682670055 1106 48-49 -0.007232176504373911 1106 50-51 -0.02737982977222231 1106 52-53 -0.050272096960270574 1106 54-55 0.013076582107920842 1106 56-57 -0.018863256732188916 1106 58-59 -0.039878419163244416 1106 60-61 -0.01606723318204928 1106 62-63 -0.03601158355443346 1106 64-65 -0.03554800527469837 1106 66-67 -0.07583190176217869 1106 68-69 -0.02625003678602411 1106 70-71 -0.07356183855510068 1106 72-73 -0.09065025290655981 1106 74-75 -0.07602029400799992 1106 76-77 -0.08383705522752649 1106 78-79 -0.07520844457117093 1106 80-81 -0.03947454340756806 1106 82-83 -0.16438228147612577 1106 84-85 -0.0395714716222777 1106 86-87 -0.04839128780055546 1106 88-89 -0.07384703451510433 1106 90-91 -0.04741323113025331 1106 92-93 0.01725016106599142 1106 94-95 -0.05144745014585439 1106 96-97 0.0054794430444502495 1106 98-99 -0.057440134178168734 1106 100-101 -0.09631043811302575 1107 1 0.005531908864846002 1107 2 0.04216512420545371 1107 3 0.07289802451042604 1107 4 0.0323998529172016 1107 5 0.07632897373370895 1107 6 0.0728204682920861 1107 7 0.052328357101103506 1107 8 0.07048088194819258 1107 9 0.10552229802810587 1107 10-11 0.061159241091402805 1107 12-13 -0.08429586247893894 1107 14-15 -0.226401338887527 1107 16-17 -0.21149767812738673 1107 18-19 0.07357224598708711 1107 20-21 0.08653065196240561 1107 22-23 -0.002386490139201669 1107 24-25 -0.10018720405992809 1107 26-27 -0.21510349233015802 1107 28-29 -0.1379546241793861 1107 30-31 -0.1034889955807401 1107 32-33 -0.051229033113983746 1107 34-35 0.04627155378233283 1107 36-37 -0.015393323414471638 1107 38-39 0.044391529945052355 1107 40-41 0.03471470808170807 1107 42-43 0.031063695109835976 1107 44-45 0.0035024230788778254 1107 46-47 0.0029037483293521404 1107 48-49 -0.01947746289287977 1107 50-51 -0.014446882383161608 1107 52-53 -0.020275277181163176 1107 54-55 -0.05316201563245926 1107 56-57 -0.08606096512024664 1107 58-59 -0.09712476840541484 1107 60-61 -0.10101302513934485 1107 62-63 -0.1486739450169594 1107 64-65 -0.07357611044653112 1107 66-67 -0.026594064393137273 1107 68-69 0.03564728655740623 1107 70-71 -0.04697959480984082 1107 72-73 -0.15728443670770176 1107 74-75 -0.0771622260773448 1107 76-77 0.015981757555454834 1107 78-79 -0.05142098946241447 1107 80-81 -0.08248008333536916 1107 82-83 -0.04414267899764468 1107 84-85 -0.04560805652711508 1107 86-87 -1.2126560887182336E-4 1107 88-89 -0.04429355310111305 1107 90-91 -0.0362765732452317 1107 92-93 -0.0522534936836081 1107 94-95 -0.0039891661677948775 1107 96-97 -0.05309085788037038 1107 98-99 -0.07210110556378524 1107 100-101 -0.2033955899626605 1108 1 -0.09031789123513789 1108 2 -0.007521566859605144 1108 3 -0.017128707033510437 1108 4 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-1.456703521508608 2216 88-89 -1.4604552830185789 2216 90-91 -1.5001979756733093 2216 92-93 -1.6585044782992888 2216 94-95 -1.7498627272397975 2216 96-97 -1.746721024604188 2216 98-99 -1.7011739879255963 2216 100-101 -1.5725262703716432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 460745.0 3 122167.0 4 117567.0 5 130951.0 6 169043.0 7 202399.0 8 238410.0 9 279991.0 10 269154.0 11 256536.0 12 265131.0 13 280255.0 14 300111.0 15 316162.0 16 332603.0 17 326543.0 18 309296.0 19 313596.0 20 327771.0 21 353361.0 22 395031.0 23 454957.0 24 537790.0 25 649835.0 26 763864.0 27 888511.0 28 1042257.0 29 1225290.0 30 1446305.0 31 1726946.0 32 2098624.0 33 2584414.0 34 3262814.0 35 4280941.0 36 5962252.0 37 8987007.0 38 1.1403584E7 39 4260567.0 40 10997.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.722159657936132 20.83981807683437 21.47266511049096 28.96535715473854 2 19.25681073746679 25.155331507674777 43.03113430167863 12.556723453179808 3 20.48914724946555 28.89047308759513 34.712270637476436 15.90810902546288 4 19.443525302154782 35.62321900991956 28.444750002143532 16.488505685782123 5 20.39823909403032 38.63390818944368 25.768822705144384 15.199030011381614 6 16.770349027568592 38.06726521929539 26.366030968157204 18.79635478497881 7 15.090504102865621 16.622965342675208 49.06683481050106 19.219695743958116 8 18.15684003400431 23.164464654686597 34.15051108861956 24.528184222689525 9 19.14498915990064 22.779627042470516 34.122566416856884 23.95281738077196 10-11 21.32643186540752 35.17806461194821 24.652771390748487 18.842732131895783 12-13 18.437469667427113 27.786465765730878 34.18018636716367 19.59587819967834 14-15 19.24133043880461 28.244346103233514 33.251627976253914 19.262695481707965 16-17 20.46669844496592 28.661277334122826 30.750864192741307 20.121160028169953 18-19 21.23882786602309 29.492831318829882 28.919058021524936 20.349282793622095 20-21 20.440444286778714 30.155336371827683 29.363662377784504 20.040556963609102 22-23 20.254200361973908 30.410237968203486 30.272294698372516 19.063266971450094 24-25 20.3752257185214 30.174633365885523 29.88011788884054 19.570023026752537 26-27 20.33190730303765 30.27391071153889 29.229764899000948 20.164417086422514 28-29 20.649855195112632 29.844331149000887 29.042419703907985 20.4633939519785 30-31 20.34131988412166 29.708213295312312 29.967235197105254 19.98323162346077 32-33 20.56818372866699 30.08608736562131 29.524030000064876 19.821698905646823 34-35 20.233704645786258 30.2812692681115 28.8866459357734 20.598380150328843 36-37 20.5633321488719 29.570733612832743 29.13186139371656 20.734072844578797 38-39 20.97421525159318 29.160970994204007 29.492443805873954 20.37236994832886 40-41 21.034890813590003 29.65572712079888 29.057935084694797 20.251446980916317 42-43 20.890698431514693 30.346214789568204 28.866260189761046 19.896826589156056 44-45 20.74740702703048 29.821491399844863 29.159711783971037 20.27138978915362 46-47 20.645321791536688 29.548640657870134 29.117641858792233 20.68839569180094 48-49 20.971076758706143 29.425167011651567 29.003750411239626 20.600005818402668 50-51 20.71822318179641 29.61449485911139 29.262780499304856 20.40450145978734 52-53 20.33556849393249 30.13471910553055 29.41321993136576 20.116492469171202 54-55 20.508127793911584 29.997461953738025 29.151101594287628 20.343308658062767 56-57 21.10603184970497 29.19759472782808 28.931178112338824 20.76519531012813 58-59 21.013478743503864 29.44138979386758 28.703481172989076 20.84165028963948 60-61 21.087035689051664 29.707460968713555 28.692974831791997 20.512528510442788 62-63 20.01647319351684 31.07760654518569 28.40721311031797 20.4987071509795 64-65 20.302287459757014 30.113544565566396 28.89072222380208 20.693445750874503 66-67 20.892090988274823 28.962311871686158 29.31006149580083 20.83553564423819 68-69 21.354844776651227 29.92766491968959 28.23719805440294 20.480292249256244 70-71 20.68152256405389 30.59459413452814 28.405139680261716 20.31874362115625 72-73 20.421404785780183 30.070961778662735 29.171760963371845 20.335872472185237 74-75 20.85344229633835 29.407805532880506 28.993433527604758 20.74531864317639 76-77 21.261051386380363 29.513097280411017 28.42529621022169 20.80055512298693 78-79 20.94016544123737 30.267562321700936 28.155675289603415 20.636596947458283 80-81 20.711591135286675 30.12376447110424 29.065799292245405 20.09884510136368 82-83 20.667492906918877 29.965246578874023 28.895466136511534 20.471794377695574 84-85 20.91668137363157 30.02737674927012 28.524950004165373 20.530991872932937 86-87 21.334145206700615 30.132054784469975 27.96093777406654 20.572862234762866 88-89 21.02887799300684 30.11938725291994 28.53711049340115 20.31462426067207 90-91 21.19016549675035 29.99927110072517 28.578249664818355 20.23231373770612 92-93 20.947810542610057 30.106813735070837 28.37663213804294 20.56874358427617 94-95 21.04878775379017 30.10843505374659 28.142077231599284 20.700699960863954 96-97 21.464787563469557 29.530707022741442 28.444965111495925 20.55954030229308 98-99 21.999093939373967 29.652656883387873 28.147740328457527 20.200508848780633 100-101 22.113893700254586 30.280734078232825 27.526225909651497 20.079146311861095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14329.0 1 11529.0 2 8718.0 3 8432.0 4 7870.5 5 7353.0 6 7096.5 7 7224.5 8 7646.0 9 8254.5 10 9473.0 11 11048.5 12 12085.0 13 13324.5 14 15531.0 15 23098.0 16 36834.5 17 44739.5 18 53291.0 19 67330.0 20 89722.0 21 131924.0 22 184995.0 23 246730.0 24 332643.5 25 442730.0 26 559198.5 27 710072.0 28 876307.0 29 1045377.5 30 1260518.5 31 1471551.5 32 1693086.0 33 1975961.0 34 2284439.5 35 2602598.0 36 2888281.5 37 2911417.5 38 2679017.0 39 2417969.0 40 2200894.0 41 2051494.5 42 1949642.0 43 1887360.5 44 1800990.5 45 1723605.0 46 1700368.5 47 1682882.5 48 1658432.0 49 1593945.5 50 1481755.5 51 1383742.0 52 1286188.5 53 1170531.5 54 1053543.5 55 934857.5 56 828454.5 57 711463.0 58 612593.0 59 527375.5 60 432712.5 61 354697.0 62 286290.5 63 216838.0 64 164859.5 65 124082.5 66 88091.5 67 66956.0 68 51706.0 69 38512.5 70 27340.5 71 18335.0 72 13775.5 73 11436.0 74 9313.5 75 6970.5 76 5090.5 77 4199.5 78 3581.0 79 2801.5 80 2154.0 81 1727.5 82 1436.5 83 1317.0 84 1166.5 85 926.5 86 845.5 87 718.0 88 476.0 89 313.5 90 242.0 91 191.0 92 150.0 93 126.5 94 113.0 95 107.0 96 104.0 97 118.0 98 144.0 99 168.0 100 516.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002116687064625455 2 0.0016703346028922453 3 0.13916781558836455 4 0.3575841159060176 5 0.39349630986820083 6 0.3922444306981835 7 0.4170344279674131 8 0.21513839942679974 9 0.1472771331646191 10-11 0.18979569227331458 12-13 8.71782151822675E-6 14-15 0.0012998271883676085 16-17 0.04785386587785028 18-19 0.002784472192921624 20-21 6.486059209560702E-4 22-23 0.0027513444711523625 24-25 0.005773813191521576 26-27 0.014539582728098575 28-29 0.027180423929527363 30-31 0.032207119816936906 32-33 0.025633882392193934 34-35 0.027338216499007268 36-37 0.031901124281647146 38-39 0.026658226420585577 40-41 0.018613420723565935 42-43 0.011236400154842459 44-45 0.003981429087374157 46-47 0.002060893006908804 48-49 0.002209967754870481 50-51 9.781395743450414E-4 52-53 0.0010958301648411025 54-55 0.0010592153144645502 56-57 3.234311783262124E-4 58-59 1.1158811543330242E-4 60-61 2.1794553795566878E-5 62-63 0.0010461385821872102 64-65 0.12238252203717076 66-67 0.21311237770596383 68-69 0.20283842504673363 70-71 0.03096570203274142 72-73 2.702524670650293E-5 74-75 0.0 76-77 1.7435643036453503E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.0461385821872101E-5 88-89 0.0 90-91 3.042519709861136E-4 92-93 1.0199851176325297E-4 94-95 0.0 96-97 8.717821518226751E-7 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.7353778E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.198542659349044 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8937246701276 33.32206927344459 2 7.61359839362183 6.121111196343171 3 2.8009651847559067 3.3778415540028544 4 1.4742783613087613 2.3705536639530185 5 0.9320371080452932 1.873326672392752 6 0.6127122856748254 1.4778084572164036 7 0.4656628147910718 1.3103276567676019 8 0.36306284082777696 1.1675677676031875 9 0.280462102640211 1.0146751017581925 >10 2.129814454342202 16.86364599300035 >50 0.24432674595858372 6.8337274462505535 >100 0.16801375170134208 13.056414500650282 >500 0.013926538744538964 3.7990291757680934 >1k 0.006772525019238109 4.877325297122306 >5k 4.295963613223404E-4 1.1674422436254597 >10k+ 2.1262607932354022E-4 1.367134000101248 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 62164 0.10838693137180955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011978286766043556 0.0 0.0 1.74356430364535E-6 0.0 2 0.001220495012551745 6.9742572145814E-6 0.0 5.230692910936051E-6 0.0 3 0.0012762890702683962 6.9742572145814E-6 0.0 6.9742572145814E-6 0.0 4 0.0015570029231552977 6.9742572145814E-6 0.0 1.2204950125517451E-5 0.0 5 0.0016302326239084023 8.71782151822675E-6 0.0 1.39485144291628E-5 0.0 6 0.0016999751960542163 8.71782151822675E-6 0.0 2.0922771643744203E-5 0.0 7 0.0017749484611109664 1.0461385821872101E-5 0.0 2.4409900251034902E-5 0.0 8 0.0018516652904713618 1.0461385821872101E-5 0.0 3.312772176926165E-5 3.4871286072907E-6 9 0.0019789454846374723 1.0461385821872101E-5 0.0 4.010197898384305E-5 3.4871286072907E-6 10-11 0.0023049920094191526 1.39485144291628E-5 0.0 4.620445404660178E-5 3.4871286072907E-6 12-13 0.0028280613005127577 1.74356430364535E-5 0.0 4.969158265389248E-5 5.230692910936051E-6 14-15 0.003396463263501142 1.74356430364535E-5 0.0 6.015296847576458E-5 5.230692910936051E-6 16-17 0.004058145916734552 1.74356430364535E-5 0.0 6.62554435385233E-5 5.230692910936051E-6 18-19 0.004729418173638012 1.74356430364535E-5 0.0 7.671682936039541E-5 5.230692910936051E-6 20-21 0.005665712204695566 1.8307425188276175E-5 0.0 8.194752227133145E-5 5.230692910936051E-6 22-23 0.006427649805388583 1.917920734009885E-5 0.0 9.153712594138087E-5 5.230692910936051E-6 24-25 0.007132921566213127 1.917920734009885E-5 0.0 1.0287029391507565E-4 5.230692910936051E-6 26-27 0.007788501744383779 2.0050989491921524E-5 0.0 1.1333167973694776E-4 6.9742572145814E-6 28-29 0.008377826479015908 2.3538118099212227E-5 0.0 1.2117771910335183E-4 8.71782151822675E-6 30-31 0.008882588344921236 2.78970288583256E-5 0.0 1.316391049252239E-4 8.71782151822675E-6 32-33 0.009415247239684892 2.78970288583256E-5 0.0 1.464594015062094E-4 1.0461385821872101E-5 34-35 0.009984520984825097 2.964059316197095E-5 0.0 1.5604900517625885E-4 1.2204950125517451E-5 36-37 0.010551179383509836 3.574306822472967E-5 0.0 1.638950445426629E-4 1.2204950125517451E-5 38-39 0.011141375900293788 4.097376113566573E-5 0.0 1.74356430364535E-4 1.2204950125517451E-5 40-41 0.011819622414411828 4.1845543287488405E-5 0.0 1.8045890542729373E-4 1.3076732277340126E-5 42-43 0.012545816946880117 4.2717325439311084E-5 0.0 1.883049447936978E-4 1.39485144291628E-5 44-45 0.013377497119718949 5.0563364805715154E-5 0.0 1.9440741985645652E-4 1.39485144291628E-5 46-47 0.014454148077219953 5.317871126118318E-5 0.0 1.9789454846374724E-4 1.39485144291628E-5 48-49 0.015881255459753673 5.405049341300585E-5 0.0 2.0835593428561932E-4 1.6563860884630825E-5 50-51 0.017962199456154397 5.4922275564828524E-5 0.0 2.1533019150020075E-4 1.917920734009885E-5 52-53 0.020921899861592378 5.92811863239419E-5 0.0 2.240480130184275E-4 1.917920734009885E-5 54-55 0.02503845518249905 6.189653277940992E-5 0.0 2.371247452957676E-4 2.0050989491921524E-5 56-57 0.0309151386679357 6.538366138670064E-5 0.0 2.4584256681399436E-4 2.0922771643744203E-5 58-59 0.03944814236997604 6.62554435385233E-5 0.0 2.519450418767531E-4 2.0922771643744203E-5 60-61 0.05173155288915754 6.62554435385233E-5 0.0 2.563039526358665E-4 2.0922771643744203E-5 62-63 0.06902248008840847 6.799900784216866E-5 0.0 2.6414999200227056E-4 2.0922771643744203E-5 64-65 0.09341843182501422 7.235791860128203E-5 0.0 2.685089027613839E-4 2.2666335947389552E-5 66-67 0.1275931639586149 7.32297007531047E-5 0.0 2.693806849132066E-4 2.2666335947389552E-5 68-69 0.17478447540107994 7.584504720857272E-5 0.0 2.7635494212778803E-4 2.2666335947389552E-5 70-71 0.23777683834533098 7.67168293603954E-5 0.0 2.8071385288690133E-4 2.2666335947389552E-5 72-73 0.3205874249469669 8.02039579676861E-5 0.0 2.868163279496601E-4 2.615346455468025E-5 74-75 0.42886015285688767 8.107574011950878E-5 0.0 2.929188030124188E-4 2.615346455468025E-5 76-77 0.567208493222539 8.369108657497681E-5 0.0 3.0076484237882286E-4 2.615346455468025E-5 78-79 0.7355409089179792 8.369108657497681E-5 0.0 3.0686731744158165E-4 2.78970288583256E-5 80-81 0.9405570457799659 8.369108657497681E-5 0.0 3.208158318707444E-4 2.78970288583256E-5 82-83 1.1923521411266056 8.456286872679948E-5 0.0 3.295336533889712E-4 2.78970288583256E-5 84-85 1.5024738213409412 8.543465087862215E-5 0.0 3.3737969275537526E-4 2.8768811010148276E-5 86-87 1.866466407845007 8.717821518226751E-5 0.0 3.417386035144886E-4 2.964059316197095E-5 88-89 2.274992242010631 8.892177948591285E-5 0.0 3.469692964254247E-4 2.964059316197095E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTATT 193820 0.0 17.124737 1 GGAAAAC 177020 0.0 16.413273 1 CTGGAAT 118320 0.0 14.732855 1 GGCGAAT 4440 0.0 14.382131 1 TTTATTC 110650 0.0 14.195057 2 CGGCAGT 7730 0.0 14.055837 1 TTAATCG 4545 0.0 14.049869 3 GTTTATT 125375 0.0 13.265263 1 CGGGTAT 3210 0.0 13.064085 1 CTTTAAT 115545 0.0 12.937928 1 GTTTTAT 102375 0.0 12.828819 1 CGGCTAT 3190 0.0 12.697832 1 AATCGAC 4300 0.0 12.63486 5 AATCGGC 3915 0.0 12.294863 5 CGGGGAT 6215 0.0 12.268182 1 CGCCGGA 2980 0.0 12.26154 46-47 CTTTTAT 106705 0.0 11.852706 1 ATCGACC 2845 0.0 11.725474 6 GGAATCG 6405 0.0 11.68105 3 CGGCAAC 9070 0.0 11.453825 1 >>END_MODULE