FastQCFastQC Report
Fri 27 May 2016
SRR1233101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57353778
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG598110.10428432456533203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC362450.022.5767651
TACGACT372950.022.0684662
TATGCCG89800.021.56593546-47
ACGACTC392250.021.027163
CGACTCA416100.019.9019014
TCTCGTA101850.017.989240-41
ATGCCGT106900.017.71657246-47
CTCGTAT109150.017.61230342-43
CGTATGC117550.016.73734544-45
CTTTATT1910300.016.6960531
TGCCGTC118850.016.45862848-49
GACTCAC570800.015.9071925
GCCGTCT123850.015.3724848-49
TCGTATG129650.015.10206342-43
GGAAAAC1790200.014.9380311
TTAATCG47100.014.7358793
CTGGAAT1184000.014.5940391
CTCACTA620100.014.5415127
CCGTCTT133950.014.52787850-51
CGGGTAT36700.014.1299891
Produced by FastQC (version 0.11.5)