FastQCFastQC Report
Fri 27 May 2016
SRR1233100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65027818
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1211690.18633410089202132No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1076050.16547533549411117No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1003380.15430011814328445No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC929600.14295420461440056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG343950.037.03321
CGACGGC139850.035.5663071
GACGGCC182800.030.7856792
ACGGCCA243550.023.100743
CTGGAAT1590850.023.0004331
GGCCAGT596000.021.330542
CTTTTAT1464950.020.232961
CACGGTC219550.020.1404021
CGGCGAG56850.019.0830751
GCCAGTG732400.017.5754383
TATTATA811300.016.6378849
CTTTATT2169550.016.5461351
TATGGCG2141550.016.0173597
TGGCGAG2112100.015.8390159
ATGGCGA2193350.015.6150728
CCAGTGA839950.015.46791654
TGAATTG1076900.015.44792652
AGTGAAT997150.015.2813316
CGACGGA53300.015.1746981
CGGACGT28800.015.1349441
Produced by FastQC (version 0.11.5)