FastQCFastQC Report
Fri 27 May 2016
SRR1233100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65027818
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1513910.2328095954257607No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1086540.1670884912669221No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1077100.16563680485173285No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC986950.15177350714735655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG339800.035.4576721
CGACGGC141350.033.880441
GACGGCC179150.030.8829462
ACGGCCA246550.022.6519183
CTGGAAT1588950.022.3354511
CACGGTC227650.020.5358091
GGCCAGT616000.019.5948682
CTTTTAT1443300.017.7886541
GCCAGTG751100.016.3228283
CGGCGAG54050.016.0856911
TATGGCG2198900.015.9734777
TGAATTG1127200.015.9321252
TGGCGAG2175100.015.9150489
TATTATA848050.015.7977789
ATGGCGA2251400.015.6578478
CTTTATT2163150.015.2886571
CGGACGT25400.015.1508041
CGGTCTT320050.015.094583
ACGGTCT312900.014.8940452
GTGAATT1292900.014.8861261
Produced by FastQC (version 0.11.5)