FastQCFastQC Report
Fri 27 May 2016
SRR1233099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99735010
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC2558750.2565548446829253No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG2354390.23606454744427258No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1401940.14056648713425707No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT1328980.13325110209544272No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1315260.13187545677290252No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1197130.12003107033327615No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1136370.11393892676202669No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1061180.10639994922545253No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC1038230.10409885154671362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG263600.030.8048251
CGGCGAG89150.030.1730351
CGACGGC100600.026.8389741
GACGGCC131450.022.7982832
GGAAAAC5147450.020.6602881
CGGACGT45750.019.708841
GCGTCGG124900.019.49889494-95
ACGACGG105750.018.9584051
CTGGAAT2976950.018.528561
CACGGTC526400.018.496823
TCACGGT540400.018.1652872
GCGCACT75150.017.7629871
TTACGCG74600.017.2326356
CTTTTAT2408450.016.665211
TACGCGC77150.016.6630547
GCGACGG43150.016.5770261
TATTATA1930650.016.0985169
GAAAAAG5910000.015.9630919
ACGGCCA190000.015.6880583
CGGCGCT88350.015.6222321
Produced by FastQC (version 0.11.5)