FastQCFastQC Report
Fri 27 May 2016
SRR1233099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99735010
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC3216840.3225386952886454No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG2469780.24763420588216714No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1508480.15124879417969678No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1505740.150974066177965No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT1475960.14798815380877786No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1201240.12044316233587382No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1190710.11938736457739363No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC1077640.10805032254972452No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1064190.10670174896458123No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG1047500.10502831453067484No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1022920.10256378377061376No Hit
GTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCA998080.10007318393009637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG262600.029.215531
CGGCGAG89600.029.1975841
CGACGGC106750.026.8280541
GACGGCC133200.023.620372
CGGACGT41150.023.3918271
GCGACGG39800.021.1920411
TTGGCGA120050.020.8391381
GGAAAAC5326650.019.3770161
TCACGGT589950.018.7826652
CTGGAAT3116800.018.4490071
CACGGTC600500.018.4363443
GCGCACT72500.018.1406841
ACGACGG108350.017.9877571
TTACGCG78350.017.3130366
CTTTTAT2617350.017.2285841
TATTATA2137700.016.9686769
TAACGTA120100.016.7789618
CGGCGCT91700.016.732821
GCGACTT80250.016.6074312
GGCGACT79050.016.4567241
Produced by FastQC (version 0.11.5)