FastQCFastQC Report
Fri 27 May 2016
SRR1233098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43920645
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1704720.38813637641250487Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1039930.23677475592628477No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA655040.14914170773220659No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT642430.1462706205703491No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA641200.14599057003830432No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT629800.14339497974130389No Hit
CTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTATT629050.14322421722176437No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC543210.12367987765207No Hit
GTCTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTA506120.1152351018524432No Hit
CTGAGAAGTAATCATTGCAGGAGCAGTGTAACTCGACAAAGCTCTCGAAT470650.10715917309502172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT7824900.048.9636151
CGCGTGT279750.048.3365361
AGAGCGT483750.043.0508469
AGATCGG495950.043.0127751
GATCGGA507850.042.2450372
ATCGGAA527450.040.8021133
CGCCGTA303600.038.92960446-47
CCGTATC320600.037.1971348-49
AAGAGCG581300.036.789838
GCGTGTT420300.036.5025942
CGGAAGA598000.036.090635
TCGGAAG604250.035.6283574
GTCGCCG331450.035.0229744-45
GCCGTAT320100.032.96685448-49
GGTCGCC315050.032.88049744-45
TGGTCGC342900.032.16658442-43
TAGGCAT219550.032.008019
TCGGTGG377800.031.91317638-39
TCTCGGT368900.031.82026736-37
AGACGGC69850.031.723979