##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233098_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43920645 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.103662389293234 33.0 31.0 34.0 28.0 34.0 2 31.43809786946435 33.0 31.0 34.0 28.0 34.0 3 31.387938086974817 34.0 31.0 34.0 27.0 34.0 4 34.70722344810738 37.0 35.0 37.0 32.0 37.0 5 34.66348108048049 37.0 35.0 37.0 32.0 37.0 6 34.70991377745022 37.0 35.0 37.0 32.0 37.0 7 34.78406357647981 37.0 35.0 37.0 32.0 37.0 8 34.78728069681126 37.0 35.0 37.0 32.0 37.0 9 36.35818098755153 39.0 37.0 39.0 32.0 39.0 10-11 36.231654350704545 39.0 37.0 39.0 32.0 39.0 12-13 36.14972787854094 39.0 37.0 39.0 32.0 39.0 14-15 37.36420367915817 40.0 38.0 41.0 31.5 41.0 16-17 37.12253253566745 40.0 37.5 41.0 31.0 41.0 18-19 36.58786240502616 40.0 36.0 41.0 29.5 41.0 20-21 36.20360257687473 40.0 35.0 41.0 28.0 41.0 22-23 35.719804194587766 40.0 35.0 41.0 26.5 41.0 24-25 35.15814939648541 40.0 35.0 41.0 21.5 41.0 26-27 34.56262664175355 39.0 35.0 41.0 16.5 41.0 28-29 34.28409343715239 40.0 35.0 41.0 9.5 41.0 30-31 34.014272820902335 40.0 34.5 41.0 7.5 41.0 32-33 33.823013664758335 40.0 34.0 41.0 3.5 41.0 34-35 33.6609357057484 40.0 34.0 41.0 2.0 41.0 36-37 33.52355206759828 40.0 34.0 41.0 2.0 41.0 38-39 33.3929520616102 39.0 34.0 41.0 2.0 41.0 40-41 33.26669049373022 39.0 34.0 41.0 2.0 41.0 42-43 33.10843046590049 39.0 33.0 41.0 2.0 41.0 44-45 32.81427417561832 39.0 33.0 41.0 2.0 41.0 46-47 32.73332477243902 39.0 33.0 41.0 2.0 41.0 48-49 32.62335367570308 39.0 33.0 41.0 2.0 41.0 50-51 32.103486298983086 38.5 32.0 40.5 2.0 41.0 52-53 32.13361826767344 38.5 32.5 40.0 2.0 41.0 54-55 32.44686587594513 39.0 33.0 41.0 2.0 41.0 56-57 32.299872360708726 39.0 33.0 41.0 2.0 41.0 58-59 32.17167609901904 39.0 32.0 41.0 2.0 41.0 60-61 32.07740200536672 39.0 32.0 41.0 2.0 41.0 62-63 31.875692979918668 38.0 32.0 41.0 2.0 41.0 64-65 31.567084602241156 38.0 31.0 40.5 2.0 41.0 66-67 31.22711777115295 37.0 31.0 40.0 2.0 41.0 68-69 30.896682380233713 37.0 31.0 40.0 2.0 41.0 70-71 30.554441641282818 36.5 30.0 39.0 2.0 41.0 72-73 30.2146302723924 36.0 30.0 39.0 2.0 41.0 74-75 29.826302038597113 35.5 29.0 39.0 2.0 40.5 76-77 29.443613999748862 35.0 29.0 37.5 2.0 40.0 78-79 29.07046854617003 35.0 29.0 37.0 2.0 39.0 80-81 28.700674500568013 35.0 29.0 37.0 2.0 39.0 82-83 28.344355359535363 35.0 28.0 36.0 2.0 39.0 84-85 27.993640519168153 35.0 27.0 36.0 2.0 37.0 86-87 27.66353087483119 35.0 26.5 35.5 2.0 37.0 88-89 27.375400771095237 34.0 26.0 35.0 2.0 36.5 90-91 27.119880457128986 34.0 26.0 35.0 2.0 36.0 92-93 26.838569697690005 34.0 25.0 35.0 2.0 36.0 94-95 26.532612510585853 34.0 24.5 35.0 2.0 36.0 96-97 26.215892298485144 34.0 23.5 35.0 2.0 35.5 98-99 25.9002383047881 34.0 19.0 35.0 2.0 35.0 100-101 24.3072306542857 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5299307765324244 1101 2 0.5850538591729553 1101 3 0.6516166294288048 1101 4 0.663409981768929 1101 5 0.7035552384948005 1101 6 0.6776978120693755 1101 7 0.6620866666448606 1101 8 0.6159862403154293 1101 9 0.706529292398919 1101 10-11 0.7343190844205694 1101 12-13 0.7683124562299284 1101 14-15 0.7636499285706364 1101 16-17 0.8638401619374747 1101 18-19 0.8957442608785513 1101 20-21 0.8489023417319643 1101 22-23 0.805110126870197 1101 24-25 0.8521563461364963 1101 26-27 0.8482429076652309 1101 28-29 0.8457219274616463 1101 30-31 0.8521226201340113 1101 32-33 0.8145875789630495 1101 34-35 0.7706736227994853 1101 36-37 0.727015799582226 1101 38-39 0.7172912173228241 1101 40-41 0.6833995021101202 1101 42-43 0.7284556753655878 1101 44-45 0.648168788011553 1101 46-47 0.6751860324575958 1101 48-49 0.6683397602694967 1101 50-51 0.6619187989584105 1101 52-53 0.580126692802942 1101 54-55 0.6037431817341457 1101 56-57 0.677792556611557 1101 58-59 0.7455227131483326 1101 60-61 0.7266092796768078 1101 62-63 0.7359096343622191 1101 64-65 0.7655421698489775 1101 66-67 0.7998144009107548 1101 68-69 0.7165774100925617 1101 70-71 0.6279061332059328 1101 72-73 0.6710268894788562 1101 74-75 0.6420225630981129 1101 76-77 0.6626181174594272 1101 78-79 0.7003412322493539 1101 80-81 0.67247405941335 1101 82-83 0.6337997169621303 1101 84-85 0.5694883002033109 1101 86-87 0.618806504595149 1101 88-89 0.6972632548375657 1101 90-91 0.6961466212138845 1101 92-93 0.6801897872969818 1101 94-95 0.7687287143539301 1101 96-97 0.8052459869373116 1101 98-99 0.7762880825047098 1101 100-101 0.8856514980698762 1102 1 0.4537505488283351 1102 2 0.47576587679243687 1102 3 0.5691975810868932 1102 4 0.5310467712427425 1102 5 0.5302053732355247 1102 6 0.5153767296360314 1102 7 0.5505235942479203 1102 8 0.5545305207309141 1102 9 0.678293796458938 1102 10-11 0.7045763483935303 1102 12-13 0.6959916227204701 1102 14-15 0.6880212040101412 1102 16-17 0.772158953952605 1102 18-19 0.7997968708333616 1102 20-21 0.7465999935251304 1102 22-23 0.7776939018509239 1102 24-25 0.7703535580667591 1102 26-27 0.8101963305971722 1102 28-29 0.7697611695888895 1102 30-31 0.8285181580277339 1102 32-33 0.7934158358351127 1102 34-35 0.8044127077829586 1102 36-37 0.7863686685865545 1102 38-39 0.6993498882162896 1102 40-41 0.7930220515706452 1102 42-43 0.7399341025393014 1102 44-45 0.6973577351694402 1102 46-47 0.7303075822571401 1102 48-49 0.7043429752728514 1102 50-51 0.6629865300858739 1102 52-53 0.6360240065519704 1102 54-55 0.7435296422831286 1102 56-57 0.7938061354576718 1102 58-59 0.8210063415253046 1102 60-61 0.8174510750475363 1102 62-63 0.7698750610190608 1102 64-65 0.8155542918138323 1102 66-67 0.8290758555389388 1102 68-69 0.8633797346404322 1102 70-71 0.7919240144753275 1102 72-73 0.7386514810286897 1102 74-75 0.7896767813419139 1102 76-77 0.7774600791143342 1102 78-79 0.8029602557549502 1102 80-81 0.7758508523283005 1102 82-83 0.7727061205853829 1102 84-85 0.7946318084290169 1102 86-87 0.7867377284751385 1102 88-89 0.7593205940900276 1102 90-91 0.7633497782678269 1102 92-93 0.7680813436076299 1102 94-95 0.737761545287583 1102 96-97 0.7998334203843527 1102 98-99 0.8307984376646509 1102 100-101 0.9133912876735799 1103 1 0.34182976829750444 1103 2 0.3789738645174232 1103 3 0.37384162249573194 1103 4 0.508845475934045 1103 5 0.37418545409752824 1103 6 0.4700883185911948 1103 7 0.49759415342997926 1103 8 0.49181850274616323 1103 9 0.5426113036778375 1103 10-11 0.5580433332948829 1103 12-13 0.5874772065416423 1103 14-15 0.6424787458817747 1103 16-17 0.6332650839455525 1103 18-19 0.5346187272132994 1103 20-21 0.5856958390353526 1103 22-23 0.584026901637074 1103 24-25 0.6525377619472863 1103 26-27 0.6799939628949474 1103 28-29 0.666717564188076 1103 30-31 0.4979540877590338 1103 32-33 0.6071533390831192 1103 34-35 0.6419191477921231 1103 36-37 0.6132461552156556 1103 38-39 0.5131808246114247 1103 40-41 0.606435427693711 1103 42-43 0.6329373968577485 1103 44-45 0.5495053128734995 1103 46-47 0.5366775878136352 1103 48-49 0.5355817593911283 1103 50-51 0.5254860142061162 1103 52-53 0.5036814939955647 1103 54-55 0.5676614600243397 1103 56-57 0.5959376916011863 1103 58-59 0.649880796642968 1103 60-61 0.6372374968427224 1103 62-63 0.6940969663539072 1103 64-65 0.6448615105246347 1103 66-67 0.6614063719024443 1103 68-69 0.6211174646670621 1103 70-71 0.6680705240867262 1103 72-73 0.6368165062803861 1103 74-75 0.6367426709999862 1103 76-77 0.6261974210062853 1103 78-79 0.5795796873655803 1103 80-81 0.6217568543057794 1103 82-83 0.6478108770599889 1103 84-85 0.5971470155109095 1103 86-87 0.6093551589026518 1103 88-89 0.6122821195383779 1103 90-91 0.6396371720893406 1103 92-93 0.6457119402544222 1103 94-95 0.6708689416353479 1103 96-97 0.7378019814975261 1103 98-99 0.7011059016083614 1103 100-101 0.7907420255725945 1104 1 0.22190381396200465 1104 2 0.2056375936507564 1104 3 0.2276989881700615 1104 4 0.4286969757062451 1104 5 0.3802907883417177 1104 6 0.397950476408127 1104 7 0.4502737016171494 1104 8 0.4570077513559454 1104 9 0.5125900988198637 1104 10-11 0.4566048369178901 1104 12-13 0.47251314424632795 1104 14-15 0.5430176753393567 1104 16-17 0.5563275048896585 1104 18-19 0.5208978361133703 1104 20-21 0.5362173883658556 1104 22-23 0.51486696771628 1104 24-25 0.5325030471652497 1104 26-27 0.572756460255853 1104 28-29 0.5377486654580466 1104 30-31 0.544625215264233 1104 32-33 0.49984412960404967 1104 34-35 0.477988523313023 1104 36-37 0.42201527942210504 1104 38-39 0.4758785621125696 1104 40-41 0.4227126627020681 1104 42-43 0.49187214003603685 1104 44-45 0.5080451419738949 1104 46-47 0.5095653898761796 1104 48-49 0.5270834745053605 1104 50-51 0.5013987272139957 1104 52-53 0.4015842984780065 1104 54-55 0.48842305815603737 1104 56-57 0.4710463664661404 1104 58-59 0.5128464740897414 1104 60-61 0.5837198248047883 1104 62-63 0.5567566899823824 1104 64-65 0.42630362559415147 1104 66-67 0.5428436420036249 1104 68-69 0.5863247467447259 1104 70-71 0.4882943698141027 1104 72-73 0.5619685613037007 1104 74-75 0.5509099136218509 1104 76-77 0.5846979816023676 1104 78-79 0.5301393336383633 1104 80-81 0.5128241326634715 1104 82-83 0.5389599708137922 1104 84-85 0.5342363491374797 1104 86-87 0.4520837327225635 1104 88-89 0.477571496656509 1104 90-91 0.49071319926684964 1104 92-93 0.5289940396701205 1104 94-95 0.45930943170422367 1104 96-97 0.5505290283786763 1104 98-99 0.5232388285468907 1104 100-101 0.3481590185246901 1105 1 0.18287501173350762 1105 2 0.23146478593169917 1105 3 0.32832908919441195 1105 4 0.3883549947382434 1105 5 0.38246259161400076 1105 6 0.3799820755310961 1105 7 0.3364005943021908 1105 8 0.35077073870142783 1105 9 0.373905382639073 1105 10-11 0.40508548360622854 1105 12-13 0.32533374602633813 1105 14-15 0.4441866368328533 1105 16-17 0.40521407825442424 1105 18-19 0.38707173366591974 1105 20-21 0.3649451648047233 1105 22-23 0.4430968246670304 1105 24-25 0.3519702669979097 1105 26-27 0.3493156845688148 1105 28-29 0.40131993935612087 1105 30-31 0.31776015880392094 1105 32-33 0.2907756732299376 1105 34-35 0.17467245100201723 1105 36-37 0.20060174603028003 1105 38-39 0.19444339987458648 1105 40-41 0.2810045087400397 1105 42-43 0.3621505846992292 1105 44-45 0.2631126564843882 1105 46-47 0.30493740228781263 1105 48-49 0.25388682656838313 1105 50-51 0.29534675687720835 1105 52-53 0.2735132126254669 1105 54-55 0.3938793755578516 1105 56-57 0.40710079061260274 1105 58-59 0.3252420818247046 1105 60-61 0.3532411005401954 1105 62-63 0.3924687727442482 1105 64-65 0.3427764485290261 1105 66-67 0.4414745653659651 1105 68-69 0.3702828029066616 1105 70-71 0.35221782055624473 1105 72-73 0.38621466148007144 1105 74-75 0.41023994623482096 1105 76-77 0.4152641140404185 1105 78-79 0.3492476607548376 1105 80-81 0.40184516823635974 1105 82-83 0.3264822683818096 1105 84-85 0.3527149610685605 1105 86-87 0.41357294209269924 1105 88-89 0.4047536071881268 1105 90-91 0.3638428989657676 1105 92-93 0.3447692206232382 1105 94-95 0.2982436134305111 1105 96-97 0.26364434657504177 1105 98-99 0.19840042982508166 1105 100-101 0.2251197762190884 1106 1 0.03195849249796723 1106 2 0.08399637537417348 1106 3 0.10965582218363679 1106 4 0.0883664790771661 1106 5 0.1366901873064421 1106 6 0.12427363329977226 1106 7 0.1730106888041405 1106 8 0.1963349520630686 1106 9 0.21742067601797288 1106 10-11 0.07686760017691086 1106 12-13 0.1450919916197364 1106 14-15 0.11975802860973772 1106 16-17 0.13323571943953993 1106 18-19 0.03967911615620068 1106 20-21 0.1271183624513057 1106 22-23 0.14740206421206636 1106 24-25 -0.013713871423910007 1106 26-27 0.042035113836625726 1106 28-29 0.07207355823887696 1106 30-31 0.12562664749410146 1106 32-33 0.10550147727733616 1106 34-35 0.005816111948909963 1106 36-37 -0.024885847868425515 1106 38-39 -0.04409686180014205 1106 40-41 -0.03384933681334701 1106 42-43 0.05754841250576703 1106 44-45 0.019349238288207005 1106 46-47 -0.0565738387679815 1106 48-49 -0.036568466511070596 1106 50-51 -0.041494561386393514 1106 52-53 -0.07477706292586817 1106 54-55 0.039046134574967084 1106 56-57 0.1412793856835748 1106 58-59 0.044993847496670014 1106 60-61 0.09001268440552934 1106 62-63 0.1644572966956197 1106 64-65 0.12994656866433374 1106 66-67 0.15496528101448348 1106 68-69 0.13684974275143702 1106 70-71 0.04189671919775151 1106 72-73 0.06965293619025559 1106 74-75 0.08869984354675253 1106 76-77 0.048669912045074426 1106 78-79 0.09920600989315531 1106 80-81 0.12306575431476219 1106 82-83 0.14859437254447982 1106 84-85 0.18458370203793706 1106 86-87 0.09067602935337504 1106 88-89 0.09367558394493258 1106 90-91 0.12655173666315633 1106 92-93 0.05799294926358556 1106 94-95 0.010114357584786404 1106 96-97 0.00606197281294385 1106 98-99 -0.1801449430307045 1106 100-101 -0.1823886105474628 1107 1 0.08068058336901629 1107 2 0.1151942202056162 1107 3 0.12852273806209524 1107 4 0.20470208422046454 1107 5 0.06873430579135942 1107 6 0.2134115350403505 1107 7 0.11863904130604652 1107 8 0.19502346818298832 1107 9 0.1302762618170945 1107 10-11 0.20210695798742506 1107 12-13 0.1996083246006819 1107 14-15 0.162503591396586 1107 16-17 0.15069920366560297 1107 18-19 0.1949781420409309 1107 20-21 0.19874038460918086 1107 22-23 0.08000145830980188 1107 24-25 0.11746726861851187 1107 26-27 0.09543582245679971 1107 28-29 0.06252962112147031 1107 30-31 0.08705819508468693 1107 32-33 0.10031615227307356 1107 34-35 0.04959367588745778 1107 36-37 0.07659997990914036 1107 38-39 0.010522518103940115 1107 40-41 0.07865414128080772 1107 42-43 0.07459579825300722 1107 44-45 0.12132714496298291 1107 46-47 0.0784062546842037 1107 48-49 0.09443008282737964 1107 50-51 -0.018957894272283227 1107 52-53 0.05451737197330431 1107 54-55 0.13216769672606432 1107 56-57 0.166661578878319 1107 58-59 0.1577909574350187 1107 60-61 0.03996629366413629 1107 62-63 0.09619940807376892 1107 64-65 0.07588344687273363 1107 66-67 0.1255441080353883 1107 68-69 0.20539483587655027 1107 70-71 0.19207896423901616 1107 72-73 0.2124139362294919 1107 74-75 0.16953797267830595 1107 76-77 0.18830420351353183 1107 78-79 0.15377733483698464 1107 80-81 0.1585994624697058 1107 82-83 0.11066418201326655 1107 84-85 0.110535976544341 1107 86-87 0.11692172367403231 1107 88-89 0.07990184516947352 1107 90-91 0.08483427224691553 1107 92-93 0.023549408001329653 1107 94-95 0.06611520912647606 1107 96-97 0.13925529163722317 1107 98-99 0.09813196873781393 1107 100-101 0.008370506996808302 1108 1 0.029305352129650686 1108 2 0.059880795271872955 1108 3 0.08867627875599382 1108 4 0.18304345119715038 1108 5 0.0457389225665068 1108 6 0.048668098021096284 1108 7 0.09306645669155245 1108 8 0.05772639135278723 1108 9 0.08294911020234252 1108 10-11 0.08791495436628338 1108 12-13 0.07800271307792173 1108 14-15 0.04915195001778727 1108 16-17 0.015761568541215354 1108 18-19 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2314 84-85 -0.3342560488212172 2314 86-87 -0.3084921420465676 2314 88-89 -0.3408938398804082 2314 90-91 -0.3813856789578338 2314 92-93 -0.4250575146086568 2314 94-95 -0.40429949059415193 2314 96-97 -0.38640159088774695 2314 98-99 -0.4014515288255076 2314 100-101 -0.508122389118423 2315 1 -0.9075567199670402 2315 2 -0.801896493592718 2315 3 -0.9818774278919733 2315 4 -1.0354524270433743 2315 5 -1.0095030555978894 2315 6 -1.0745877899396703 2315 7 -1.0377206765887266 2315 8 -0.9594352900710064 2315 9 -1.1704575854125423 2315 10-11 -1.2412379081189968 2315 12-13 -1.2846190565245692 2315 14-15 -1.5635123755559306 2315 16-17 -1.586883461425451 2315 18-19 -1.4805829158932937 2315 20-21 -1.583521512539619 2315 22-23 -1.640532992776194 2315 24-25 -1.7004457657248508 2315 26-27 -1.816147931362913 2315 28-29 -1.870412322758007 2315 30-31 -1.8218943511798642 2315 32-33 -1.7340919563970445 2315 34-35 -1.6401635727010486 2315 36-37 -1.6221235216105185 2315 38-39 -1.581608644755324 2315 40-41 -1.6008892146505076 2315 42-43 -1.6636984274508713 2315 44-45 -1.5819277854963865 2315 46-47 -1.6187120684842213 2315 48-49 -1.6686282497018468 2315 50-51 -1.6660996925715672 2315 52-53 -1.5733646443449345 2315 54-55 -1.5655846998035443 2315 56-57 -1.502739638496955 2315 58-59 -1.5046321833598668 2315 60-61 -1.669706331983683 2315 62-63 -1.6302967897586136 2315 64-65 -1.596685440471127 2315 66-67 -1.5380295130819732 2315 68-69 -1.6373070656560351 2315 70-71 -1.6916320152885334 2315 72-73 -1.6288083583186328 2315 74-75 -1.6711015686541657 2315 76-77 -1.6700029363718052 2315 78-79 -1.67832522841206 2315 80-81 -1.61785821886075 2315 82-83 -1.6446727683049254 2315 84-85 -1.7692891294039406 2315 86-87 -1.7463439954952875 2315 88-89 -1.7574446988054149 2315 90-91 -1.759607753942035 2315 92-93 -1.7667468706322147 2315 94-95 -1.8284378626383635 2315 96-97 -2.237066224676635 2315 98-99 -2.3058344686549184 2315 100-101 -2.3340559745971987 2316 1 -2.53169392844519 2316 2 -2.889493597221808 2316 3 -2.7578904848300425 2316 4 -2.9544489273339707 2316 5 -2.9028310168503424 2316 6 -2.9420241245238863 2316 7 -2.7967793071703966 2316 8 -2.850840676324623 2316 9 -3.3466878745395476 2316 10-11 -3.557196722578418 2316 12-13 -3.493691804706522 2316 14-15 -4.058007947678803 2316 16-17 -3.8070260364247233 2316 18-19 -3.867861057852238 2316 20-21 -3.9485278742780707 2316 22-23 -3.999786904802061 2316 24-25 -3.873631641530828 2316 26-27 -3.8595415334347436 2316 28-29 -4.040313529716368 2316 30-31 -4.057900463807918 2316 32-33 -4.033830331254116 2316 34-35 -4.0720176584929675 2316 36-37 -3.985169945838969 2316 38-39 -3.8911383808291475 2316 40-41 -4.012631065383342 2316 42-43 -4.196798568762119 2316 44-45 -4.056036077298494 2316 46-47 -4.144251610925593 2316 48-49 -4.14900581108035 2316 50-51 -4.048068681050427 2316 52-53 -3.9827634156023812 2316 54-55 -4.166037406919905 2316 56-57 -4.136581750021158 2316 58-59 -4.230220610833108 2316 60-61 -4.472223856022325 2316 62-63 -4.386050124677091 2316 64-65 -4.328057871695538 2316 66-67 -4.262353431144959 2316 68-69 -4.187062592853369 2316 70-71 -4.202119578293349 2316 72-73 -4.154675809288445 2316 74-75 -4.140764970623753 2316 76-77 -4.1348882714778235 2316 78-79 -4.140462987492395 2316 80-81 -3.9042038329345914 2316 82-83 -3.9645238945755743 2316 84-85 -4.068983186239347 2316 86-87 -4.086429435958461 2316 88-89 -4.1528375601591385 2316 90-91 -4.181105597202244 2316 92-93 -4.176300907154204 2316 94-95 -4.368858712208404 2316 96-97 -4.51199877358027 2316 98-99 -4.663088553615122 2316 100-101 -4.95586406635012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 500761.0 3 132588.0 4 123579.0 5 137167.0 6 184023.0 7 301279.0 8 534604.0 9 921510.0 10 1054024.0 11 757971.0 12 475217.0 13 351846.0 14 291363.0 15 256613.0 16 235903.0 17 228312.0 18 229194.0 19 237945.0 20 255819.0 21 278248.0 22 306696.0 23 342194.0 24 383816.0 25 394767.0 26 407077.0 27 453612.0 28 515515.0 29 595028.0 30 696789.0 31 830189.0 32 1016061.0 33 1278130.0 34 1676856.0 35 2367777.0 36 3695017.0 37 6971823.0 38 1.0616769E7 39 3871713.0 40 12850.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.631557925147337 18.096166058043504 9.361340061597437 44.91093595521172 2 12.863266980264015 16.32991972923535 60.81417375656375 9.992639533936888 3 14.036883146724147 23.279994180228147 42.38709545533605 20.296027217711657 4 17.420751887753102 29.355710643643185 36.48672121401865 16.73681625458506 5 18.902874916011108 31.786184675720065 35.78264166392701 13.528298744341816 6 14.128962206112497 35.023323235584705 37.21454730880234 13.633167249500458 7 12.850166755603446 19.11026764021784 54.24989442867321 13.789671175505507 8 12.175137078361391 21.778276485980708 47.879656406175435 18.166930029482465 9 13.819014576011282 22.64106999279713 46.55688470481037 16.98303072638121 10-11 17.614883340752332 31.82096285448685 35.50335351258695 15.060800292173864 12-13 16.51294459927159 25.28017767175094 41.64764980648593 16.559227922491534 14-15 16.78069105619889 27.65467530487581 40.68082416547344 14.883809473451864 16-17 17.572339874050652 27.250199340677593 39.211230711399644 15.966230073872111 18-19 17.3224299024915 28.17584793288921 38.5173920674265 15.984330097192798 20-21 18.209470666997156 28.474909881853677 37.48120725105251 15.83441220009666 22-23 18.756031313343293 29.035985288712823 36.343835085044404 15.864148312899475 24-25 17.967753232590862 30.435297531983295 35.01202492161669 16.584924313809154 26-27 17.9954089085512 30.644710899115164 34.78903137641168 16.570848815921956 28-29 18.15448163516209 30.838744509880406 34.10373534655717 16.90303850840033 30-31 18.179963869751255 31.4782357008841 33.639973484135865 16.70182694522878 32-33 18.034142262119907 30.77376252846185 33.97935960909964 17.2127356003186 34-35 18.553852530403287 31.469415569379745 32.93961210208504 17.03711979813193 36-37 17.8370409215834 31.36667074371719 33.76119975395384 17.035088580745565 38-39 17.916290805561577 30.76458724247128 33.91324619376771 17.40587575819943 40-41 18.31452459054267 30.41292455170766 33.96318443875428 17.309366418995385 42-43 18.248784061804884 30.694404241841134 34.13601067984318 16.9208010165108 44-45 18.78435322874399 30.247858051621034 33.93833989059505 17.02944882903992 46-47 18.157998148385907 30.18804893525806 34.058675194589384 17.59527772176665 48-49 18.06075142790544 30.979497882338336 33.237705235177096 17.722045454579128 50-51 17.877690680528477 30.756556610914938 33.946025396611496 17.419727311945092 52-53 18.182789656869247 31.439327778144477 33.01211581015796 17.365766754828314 54-55 18.017770086626587 31.75561871182728 32.296896745819105 17.92971445572703 56-57 18.281493346790334 31.990618106919396 32.490503141021755 17.23738540526852 58-59 17.99256386206851 32.39379082916336 32.087345739631154 17.526299569136977 60-61 18.14767285753262 32.53686068586524 31.676498347336686 17.638968109265452 62-63 18.285472757925838 32.883104994979966 31.662122524520615 17.16929972257358 64-65 18.336151963589035 32.32889823752723 31.8748947164618 17.46005508242194 66-67 18.564825151408783 32.722262169568566 30.963897876741164 17.749014802281486 68-69 18.46579988727673 32.61389768837534 31.817230962530964 17.103071461816967 70-71 18.580356093201132 32.10369190889428 31.53880994147955 17.777142056425042 72-73 18.068662676252593 32.571763055668804 31.501369678992226 17.858204589086384 74-75 18.25470853571574 31.5740405908664 32.55306066930562 17.618190204112242 76-77 17.877139305731326 32.35890466789905 31.86577633534855 17.898179691021078 78-79 18.214753039076363 32.45075767504248 31.440322192807415 17.894167093073744 80-81 18.202879566325887 31.891236145392575 32.00419984141712 17.901684446864422 82-83 18.643618984856523 31.747841594157723 31.540827641084817 18.06771177990094 84-85 18.292059079233535 31.478247050166928 31.842946350961203 18.386747519638334 86-87 18.39663411411527 32.21503657593822 31.651736211095617 17.736593098850896 88-89 18.291032132511635 31.547709643468213 32.13461284597403 18.026645378046123 90-91 18.396186299421426 32.17417517698286 31.770320902696614 17.659317620899106 92-93 18.64565953371569 31.963163352700484 31.712262719709884 17.67891439387394 94-95 18.420860262455495 31.74416712426537 31.83670987327182 17.998262740007316 96-97 18.262374838924604 32.21904959180254 31.463578179482894 18.054997389789968 98-99 18.18997213091392 32.03820383132622 31.83457458749624 17.93724945026362 100-101 18.74136508206719 32.77110350400646 30.70331420100886 17.78421721291749 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16973.0 1 31506.0 2 43234.5 3 46989.5 4 61479.5 5 79265.5 6 98294.5 7 118391.5 8 138946.0 9 157221.5 10 173319.0 11 187088.5 12 198659.5 13 208105.5 14 217480.0 15 226636.5 16 233460.0 17 241540.0 18 256561.0 19 276464.5 20 306880.5 21 350139.0 22 407327.0 23 465213.5 24 560699.5 25 690304.5 26 845998.5 27 1075700.5 28 1351144.5 29 1465771.0 30 1487103.5 31 1609389.0 32 1779215.0 33 1885928.0 34 1926498.5 35 1970813.5 36 1944927.5 37 1958355.5 38 1982489.5 39 1805011.0 40 1612754.5 41 1464525.5 42 1291592.0 43 1191733.5 44 1094945.0 45 1000987.0 46 934451.0 47 887648.0 48 862818.5 49 823681.0 50 714665.0 51 585314.0 52 503731.5 53 433721.0 54 355813.0 55 270846.5 56 209313.0 57 164033.5 58 128377.5 59 106287.0 60 86789.5 61 64082.0 62 45320.5 63 33900.0 64 23720.0 65 17176.0 66 13449.0 67 10922.5 68 7702.0 69 5408.5 70 6589.0 71 10464.0 72 10230.0 73 15492.5 74 17338.0 75 9807.0 76 7214.0 77 7840.5 78 7573.0 79 4586.0 80 2598.0 81 1431.0 82 811.5 83 420.0 84 198.5 85 92.0 86 66.5 87 46.0 88 27.5 89 19.0 90 14.5 91 14.5 92 9.0 93 5.0 94 4.0 95 3.0 96 1.0 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6719573448887192 2 0.3175044446637794 3 0.316161112843402 4 0.35318925758034747 5 0.34146811823915607 6 0.1787974652922333 7 0.16198304920157705 8 0.17058264968558637 9 0.16898431250269663 10-11 0.17817816655470337 12-13 0.19398963744726425 14-15 0.1863622449078332 16-17 0.17185426124775716 18-19 0.18718076658482588 20-21 0.2153110456369664 22-23 0.1707431664539535 24-25 0.18504737350737904 26-27 0.17540726007097573 28-29 0.17245648373333314 30-31 0.191814122948331 32-33 0.14652221068247062 34-35 0.17612901632022937 36-37 0.16754763050497096 38-39 0.16999067295118275 40-41 0.1676102434288021 42-43 0.13934107752743613 44-45 0.5000006261292383 46-47 0.1316772556505033 48-49 0.1094690207759927 50-51 0.11184489663118563 52-53 0.5530929702876631 54-55 0.38164853908680074 56-57 0.2797647894287527 58-59 0.2345297980027388 60-61 0.0668706026516687 62-63 0.070557934656925 64-65 0.06884347896074841 66-67 0.07833104454636311 68-69 0.09450225514675388 70-71 0.104112769746437 72-73 0.13744789039414151 74-75 0.11033535595845644 76-77 0.09818047981763475 78-79 0.1054060112277495 80-81 0.09852769694069839 82-83 0.08288812698447393 84-85 0.08601991159282839 86-87 0.10030248872711228 88-89 0.09375431531117086 90-91 0.11354569132579907 92-93 0.12357514330675243 94-95 0.14361355576631443 96-97 0.10651255235436546 98-99 0.13057185294068427 100-101 0.14368186077413025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.3920645E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.92587700827572 #Duplication Level Percentage of deduplicated Percentage of total 1 81.16215944060482 19.41875844501975 2 7.54580929282972 3.6108021013629568 3 2.861793784606081 2.05412778340599 4 1.5343993498957071 1.4684740050873164 5 1.0004339225043308 1.196812949237273 6 0.715362430465622 1.0269404116596998 7 0.5536004015319124 0.9271762583149212 8 0.4423577301184478 0.8467037315579206 9 0.3664698004588133 0.7891300235722422 >10 2.927104433691996 14.438782791241511 >50 0.43747511917528475 7.347688168322747 >100 0.3797313960547378 18.255683345939403 >500 0.0434154936728047 7.10970588188299 >1k 0.026594462520195802 11.975727958784368 >5k 0.0020640889318811824 3.367659835252454 >10k+ 0.0012288529377050986 6.165826309358754 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 170472 0.38813637641250487 Illumina Single End PCR Primer 1 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103993 0.23677475592628477 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 65504 0.14914170773220659 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 64243 0.1462706205703491 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 64120 0.14599057003830432 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 62980 0.14339497974130389 No Hit CTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTATT 62905 0.14322421722176437 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 54321 0.12367987765207 No Hit GTCTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTA 50612 0.1152351018524432 No Hit CTGAGAAGTAATCATTGCAGGAGCAGTGTAACTCGACAAAGCTCTCGAAT 47065 0.10715917309502172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.5004662385991827 1.5937835157020123E-5 0.0 2.2768335938600173E-6 2.2768335938600173E-6 2 0.5054274590002037 4.5536671877200345E-5 0.0 4.5536671877200345E-6 2.2768335938600173E-6 3 0.5056483118588081 4.5536671877200345E-5 0.0 4.5536671877200345E-6 2.2768335938600173E-6 4 0.5177428519093925 4.5536671877200345E-5 0.0 4.5536671877200345E-6 2.2768335938600173E-6 5 0.5182915688055127 4.5536671877200345E-5 0.0 9.107334375440069E-6 2.2768335938600173E-6 6 0.519133997235241 4.5536671877200345E-5 0.0 9.107334375440069E-6 2.2768335938600173E-6 7 0.519914951157935 4.5536671877200345E-5 0.0 9.107334375440069E-6 2.2768335938600173E-6 8 0.5243001326597093 4.5536671877200345E-5 0.0 1.1384167969300086E-5 2.2768335938600173E-6 9 0.5249672449027103 4.7813505471060364E-5 0.0 1.1384167969300086E-5 2.2768335938600173E-6 10-11 0.5283631422079524 5.350558945571041E-5 0.0 1.3661001563160104E-5 3.415250390790026E-6 12-13 0.5309689782561253 6.375134062808048E-5 1.1384167969300086E-6 1.8214668750880138E-5 4.5536671877200345E-6 14-15 0.5329316088140327 6.60281742219405E-5 2.2768335938600173E-6 2.0491502344740157E-5 4.5536671877200345E-6 16-17 0.5357025152977604 6.830500781580051E-5 2.2768335938600173E-6 2.8460419923250217E-5 4.5536671877200345E-6 18-19 0.5386623989697783 8.082759258203061E-5 2.2768335938600173E-6 3.187567031404024E-5 4.5536671877200345E-6 20-21 0.542051465774239 9.676542773905073E-5 2.2768335938600173E-6 3.6429337501760276E-5 4.5536671877200345E-6 22-23 0.5454211794931518 1.0701117891142082E-4 2.2768335938600173E-6 3.6429337501760276E-5 4.5536671877200345E-6 24-25 0.5477424113420921 1.1156484609914084E-4 2.2768335938600173E-6 3.984458789255031E-5 4.5536671877200345E-6 26-27 0.54967885831367 1.6051676836713124E-4 2.2768335938600173E-6 4.895192226799037E-5 4.5536671877200345E-6 28-29 0.5518691722309633 1.707625195395013E-4 3.415250390790026E-6 5.2367172658780396E-5 4.5536671877200345E-6 30-31 0.5540913618185708 2.709431976693421E-4 4.5536671877200345E-6 5.578242304957042E-5 4.5536671877200345E-6 32-33 0.5562759836518794 2.9484995040487223E-4 6.830500781580052E-6 5.8059256643430446E-5 4.5536671877200345E-6 34-35 0.5596274827020413 3.028188679833823E-4 7.96891757851006E-6 6.261292383115047E-5 4.5536671877200345E-6 36-37 0.5619043162959014 3.255872039219825E-4 9.107334375440069E-6 6.375134062808049E-5 4.5536671877200345E-6 38-39 0.5651738493366844 3.449402894697926E-4 9.107334375440069E-6 6.60281742219405E-5 4.5536671877200345E-6 40-41 0.5687632774974047 3.574628742360227E-4 1.0245751172370079E-5 6.60281742219405E-5 4.5536671877200345E-6 42-43 0.5717436526717674 3.665702086114628E-4 1.2522584766230094E-5 7.058184140966054E-5 4.5536671877200345E-6 44-45 0.5750962901387264 3.825080437684829E-4 1.3661001563160104E-5 7.513550859738056E-5 4.5536671877200345E-6 46-47 0.5808487102136137 4.041379629101531E-4 1.3661001563160104E-5 8.196600937896063E-5 4.5536671877200345E-6 48-49 0.5846680985673138 4.052763797070831E-4 1.3661001563160104E-5 8.196600937896063E-5 4.5536671877200345E-6 50-51 0.5886741872757105 4.086916300978731E-4 1.3661001563160104E-5 8.651967656668065E-5 4.5536671877200345E-6 52-53 0.5927041827368428 4.1779896447331316E-4 1.3661001563160104E-5 9.676542773905073E-5 4.5536671877200345E-6 54-55 0.5969584463069703 4.2007579806717317E-4 1.3661001563160104E-5 9.790384453598074E-5 4.5536671877200345E-6 56-57 0.6016760455134482 4.2349104845796324E-4 1.3661001563160104E-5 9.790384453598074E-5 4.5536671877200345E-6 58-59 0.6066144975785306 4.2349104845796324E-4 1.3661001563160104E-5 9.790384453598074E-5 4.5536671877200345E-6 60-61 0.6120356383655112 4.2349104845796324E-4 1.3661001563160104E-5 9.790384453598074E-5 4.5536671877200345E-6 62-63 0.6179736203782982 4.2804471564568327E-4 1.3661001563160104E-5 9.790384453598074E-5 4.5536671877200345E-6 64-65 0.6244056752809527 4.325983828334033E-4 1.3661001563160104E-5 9.904226133291075E-5 4.5536671877200345E-6 66-67 0.6312566675648775 4.4056730041191333E-4 1.5937835157020123E-5 1.0245751172370078E-4 6.830500781580052E-6 68-69 0.6389227662754042 4.4967463478735344E-4 1.5937835157020123E-5 1.0359592852063079E-4 9.107334375440069E-6 70-71 0.6476407621062943 4.5081305158428344E-4 1.5937835157020123E-5 1.0473434531756079E-4 9.107334375440069E-6 72-73 0.6573662568024672 4.5081305158428344E-4 1.5937835157020123E-5 1.0587276211449081E-4 9.107334375440069E-6 74-75 0.668674150846373 4.5195146838121345E-4 1.5937835157020123E-5 1.1042642930221084E-4 9.107334375440069E-6 76-77 0.6811124927696304 4.5308988517814346E-4 1.5937835157020123E-5 1.28641098053091E-4 9.107334375440069E-6 78-79 0.6947917090015413 4.599203859597235E-4 1.5937835157020123E-5 1.3433318203774102E-4 9.107334375440069E-6 80-81 0.7107864649984079 4.667508867413036E-4 1.5937835157020123E-5 1.457173500070411E-4 9.107334375440069E-6 82-83 0.7287472668035726 4.7585822111674363E-4 1.5937835157020123E-5 1.559631011794112E-4 9.107334375440069E-6 84-85 0.7506401602253336 4.838271386952537E-4 1.7076251953950132E-5 1.571015179763412E-4 9.107334375440069E-6 86-87 0.7755931179972425 4.974881402584137E-4 1.8214668750880138E-5 1.571015179763412E-4 9.107334375440069E-6 88-89 0.8042322693576107 4.997649738522738E-4 1.9353085547810148E-5 1.571015179763412E-4 9.107334375440069E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTTTT 782490 0.0 48.963615 1 CGCGTGT 27975 0.0 48.336536 1 AGAGCGT 48375 0.0 43.050846 9 AGATCGG 49595 0.0 43.012775 1 GATCGGA 50785 0.0 42.245037 2 ATCGGAA 52745 0.0 40.802113 3 CGCCGTA 30360 0.0 38.929604 46-47 CCGTATC 32060 0.0 37.19713 48-49 AAGAGCG 58130 0.0 36.78983 8 GCGTGTT 42030 0.0 36.502594 2 CGGAAGA 59800 0.0 36.09063 5 TCGGAAG 60425 0.0 35.628357 4 GTCGCCG 33145 0.0 35.02297 44-45 GCCGTAT 32010 0.0 32.966854 48-49 GGTCGCC 31505 0.0 32.880497 44-45 TGGTCGC 34290 0.0 32.166584 42-43 TAGGCAT 21955 0.0 32.00801 9 TCGGTGG 37780 0.0 31.913176 38-39 TCTCGGT 36890 0.0 31.820267 36-37 AGACGGC 6985 0.0 31.72397 9 >>END_MODULE