FastQCFastQC Report
Fri 27 May 2016
SRR1233098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43920645
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1738510.39582979712615785TruSeq Adapter, Index 5 (100% over 49bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT864810.19690284603060818No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT663630.1510975077893323No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA609150.13869331836998294No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC592140.13482042442682707TruSeq Adapter, Index 5 (100% over 50bp)
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT581750.1324547943228065No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA556550.12671717366627927No Hit
CTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTATT529580.1205765534636388No Hit
CTGAGAAGTAATCATTGCAGGAGCAGTGTAACTCGACAAAGCTCTCGAAT503150.11455888227506676No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC466520.10621884082075753No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA456980.10404674157221507No Hit
GTCTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTA446010.10154905511975063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT282200.048.7003941
GTTTTTT6949550.046.2861631
AGATCGG479900.043.2583161
GATCGGA525900.040.338512
ATCGGAA549750.038.5727653
GCGTGTT422350.037.1039472
AGAGCAC594650.036.1782769
TATGCCG417150.034.5744846-47
TGCCGTC407600.034.4687648-49
CGGAAGA634450.034.1244285
TCGGAAG639750.033.4521144
CCGTCTT422800.033.31278650-51
ATCTCGT415700.032.28738840-41
CGTCTGA492450.029.81477416-17
CTCGTAT469000.029.40375942-43
TAGGCAT204450.029.4009829
CCAGTCA499200.029.22900826-27
CACACGT502700.029.12666112-13
AGTGATC457900.028.8583536-37
CGTGTTT502100.028.688073
Produced by FastQC (version 0.11.5)