##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43920645 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.088901699872576 31.0 31.0 34.0 25.0 34.0 2 30.955375951332226 33.0 31.0 34.0 27.0 34.0 3 30.83721464017662 34.0 31.0 34.0 27.0 34.0 4 34.166496962874746 37.0 35.0 37.0 30.0 37.0 5 34.107684165385095 37.0 35.0 37.0 30.0 37.0 6 34.27461329404429 37.0 35.0 37.0 31.0 37.0 7 34.401156563160676 37.0 35.0 37.0 31.0 37.0 8 34.39742676820889 37.0 35.0 37.0 31.0 37.0 9 35.99587808876668 39.0 37.0 39.0 31.0 39.0 10-11 35.83566226087982 39.0 37.0 39.0 30.5 39.0 12-13 35.80820906660182 39.0 37.0 39.0 30.0 39.0 14-15 37.098894984351894 40.0 38.0 41.0 31.0 41.0 16-17 36.908753491666616 40.0 37.5 41.0 30.5 41.0 18-19 36.47369327112568 40.0 36.5 41.0 30.0 41.0 20-21 36.06536280375664 40.0 36.0 41.0 28.0 41.0 22-23 35.7447617971002 40.0 36.0 41.0 26.5 41.0 24-25 35.322357731768285 40.0 36.0 41.0 22.5 41.0 26-27 34.79602023148795 40.0 35.0 41.0 13.5 41.0 28-29 34.37326085488954 40.0 35.0 41.0 5.0 41.0 30-31 34.06247878417997 40.0 35.0 41.0 2.0 41.0 32-33 33.79737909131344 40.0 35.0 41.0 2.0 41.0 34-35 33.63402378312067 40.0 35.0 41.0 2.0 41.0 36-37 33.685521045968244 40.0 35.0 41.0 2.0 41.0 38-39 33.63651838446361 40.0 35.0 41.0 2.0 41.0 40-41 33.53769265000548 40.0 35.0 41.0 2.0 41.0 42-43 33.41183481025837 40.0 34.5 41.0 2.0 41.0 44-45 33.324631320874275 40.0 34.0 41.0 2.0 41.0 46-47 33.20439809114825 40.0 34.0 41.0 2.0 41.0 48-49 33.11277050917627 40.0 34.0 41.0 2.0 41.0 50-51 33.01312202951482 40.0 34.0 41.0 2.0 41.0 52-53 32.755312325217446 39.0 33.0 41.0 2.0 41.0 54-55 32.638254356237255 39.0 33.0 41.0 2.0 41.0 56-57 32.53075159756875 39.0 33.0 41.0 2.0 41.0 58-59 32.350824367447245 39.0 33.0 41.0 2.0 41.0 60-61 32.28100599387828 39.0 33.0 41.0 2.0 41.0 62-63 32.13319789588701 38.5 33.0 41.0 2.0 41.0 64-65 31.765410924634644 38.0 32.0 40.0 2.0 41.0 66-67 31.559783548260732 37.5 32.0 40.0 2.0 41.0 68-69 31.104777753150028 37.0 31.0 40.0 2.0 41.0 70-71 30.783209445125408 36.5 31.0 39.5 2.0 41.0 72-73 30.47470199948111 36.0 31.0 39.0 2.0 41.0 74-75 30.156074518031325 35.5 31.0 39.0 2.0 40.5 76-77 29.087563149402747 34.5 29.0 37.0 2.0 39.0 78-79 29.473800202615422 35.0 30.0 37.0 2.0 39.0 80-81 29.291337797976325 35.0 30.0 37.0 2.0 39.0 82-83 29.004424593491287 35.0 30.0 36.5 2.0 39.0 84-85 28.712439252656694 35.0 30.0 36.0 2.0 37.0 86-87 28.41349034150113 35.0 29.0 36.0 2.0 37.0 88-89 28.174162219156845 35.0 29.0 35.0 2.0 37.0 90-91 27.937589805432047 35.0 29.0 35.0 2.0 36.0 92-93 27.716288911513026 35.0 29.0 35.0 2.0 36.0 94-95 27.50270584596378 34.5 29.0 35.0 2.0 36.0 96-97 27.291737518882066 34.0 28.5 35.0 2.0 36.0 98-99 27.031861394567407 34.0 27.0 35.0 2.0 35.5 100-101 26.092950706894218 33.5 23.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0895691002130796 1101 2 0.5614441304874909 1101 3 0.779894167323107 1101 4 0.969184727217133 1101 5 0.9069221576853224 1101 6 0.7108819153590602 1101 7 0.6131322201481098 1101 8 0.6185269903609694 1101 9 0.7535957580623531 1101 10-11 0.7671494523163958 1101 12-13 0.7522040392240257 1101 14-15 0.8203567938941845 1101 16-17 0.7845998085814543 1101 18-19 0.7401160857802296 1101 20-21 0.7449190551919855 1101 22-23 0.6725365012038154 1101 24-25 0.7260526038536028 1101 26-27 0.7089684824015663 1101 28-29 0.6850644305715505 1101 30-31 0.7366360637743128 1101 32-33 0.7491835049281121 1101 34-35 0.7201395179131325 1101 36-37 0.6422116353069853 1101 38-39 0.6633933652950432 1101 40-41 0.62613468415217 1101 42-43 0.655386254090196 1101 44-45 0.687137248922923 1101 46-47 0.6484528058871035 1101 48-49 0.7000003615146113 1101 50-51 0.6358806436877487 1101 52-53 0.7541862616589086 1101 54-55 0.7460547679404854 1101 56-57 0.6171679607731875 1101 58-59 0.5741281382800523 1101 60-61 0.5154394052753872 1101 62-63 0.5688928915152616 1101 64-65 0.5896024839344278 1101 66-67 0.6218866309388034 1101 68-69 0.45299623913112796 1101 70-71 0.6219904212356582 1101 72-73 0.7033068920542895 1101 74-75 0.6287516686941323 1101 76-77 0.5550717239182141 1101 78-79 0.544555396895877 1101 80-81 0.5031448145322734 1101 82-83 0.604226029108414 1101 84-85 0.7100561536769874 1101 86-87 0.7194000699675946 1101 88-89 0.7405029222541586 1101 90-91 0.7391187210786008 1101 92-93 0.7364300226167018 1101 94-95 0.7099667948848101 1101 96-97 0.7578880911708232 1101 98-99 0.7848020632990611 1101 100-101 0.8747021523722829 1102 1 1.4163912872673627 1102 2 0.8528680850226742 1102 3 1.0277762976117444 1102 4 1.0233982268573243 1102 5 0.9194605326350782 1102 6 0.6969366285180811 1102 7 0.6134333296955106 1102 8 0.5867842333673039 1102 9 0.7026778019145254 1102 10-11 0.7458077685066087 1102 12-13 0.6991399725901388 1102 14-15 0.7682409827858692 1102 16-17 0.6673958606329506 1102 18-19 0.7021509923869615 1102 20-21 0.7093728441659906 1102 22-23 0.7010943105436311 1102 24-25 0.6666958712419699 1102 26-27 0.6499908225687534 1102 28-29 0.6509562569135241 1102 30-31 0.6297220888914552 1102 32-33 0.5665759785816178 1102 34-35 0.6004856299922139 1102 36-37 0.5681966191900827 1102 38-39 0.5872023180950023 1102 40-41 0.5947156094081976 1102 42-43 0.5439026742560245 1102 44-45 0.608681382837851 1102 46-47 0.5763937617441712 1102 48-49 0.5450479910931136 1102 50-51 0.5417853341611263 1102 52-53 0.6466633430001067 1102 54-55 0.602568840278856 1102 56-57 0.5594994025456614 1102 58-59 0.5271838499186359 1102 60-61 0.592987887548091 1102 62-63 0.5795608307987337 1102 64-65 0.6329120778524739 1102 66-67 0.6451713080213466 1102 68-69 0.4553933198049229 1102 70-71 0.5744160042752995 1102 72-73 0.6027790150818895 1102 74-75 0.6042982621354263 1102 76-77 0.5707388703946705 1102 78-79 0.6015333621166903 1102 80-81 0.5965611431236262 1102 82-83 0.5944396954285942 1102 84-85 0.5581015705020604 1102 86-87 0.5075717304500991 1102 88-89 0.5063426156506168 1102 90-91 0.546376349844973 1102 92-93 0.5858796973627172 1102 94-95 0.6029045186291668 1102 96-97 0.6213998122266382 1102 98-99 0.6286435001751158 1102 100-101 0.6202549556156107 1103 1 1.3793244439799501 1103 2 0.8579879375646087 1103 3 1.0323683862995026 1103 4 1.0293768730549004 1103 5 0.9309994397528314 1103 6 0.7128488293788635 1103 7 0.6338019185176478 1103 8 0.5561593009906147 1103 9 0.6215219638976279 1103 10-11 0.7157952613163303 1103 12-13 0.6878404584256756 1103 14-15 0.7267793029679623 1103 16-17 0.7106645897902268 1103 18-19 0.6873576215540211 1103 20-21 0.6708716470043399 1103 22-23 0.6750108431632356 1103 24-25 0.7031784218108683 1103 26-27 0.7180403024950337 1103 28-29 0.756189961590799 1103 30-31 0.7659307951750378 1103 32-33 0.7852250037369259 1103 34-35 0.7614910955613041 1103 36-37 0.7557177522177909 1103 38-39 0.7459139979513054 1103 40-41 0.6801409639111213 1103 42-43 0.7313431134133381 1103 44-45 0.7495117237882241 1103 46-47 0.7822648979547537 1103 48-49 0.7673283967618119 1103 50-51 0.7630735781373588 1103 52-53 0.881720279265295 1103 54-55 0.7813601574745519 1103 56-57 0.7357223331277467 1103 58-59 0.7488698299627643 1103 60-61 0.7958615331987104 1103 62-63 0.7448052007985027 1103 64-65 0.8554696944498943 1103 66-67 0.755930724883136 1103 68-69 0.6216155295633818 1103 70-71 0.739257496781363 1103 72-73 0.6722858396197218 1103 74-75 0.6887354449605034 1103 76-77 0.6564978625935822 1103 78-79 0.7427127583355855 1103 80-81 0.7873043638175439 1103 82-83 0.7575884821375602 1103 84-85 0.717629162937989 1103 86-87 0.7102438611620165 1103 88-89 0.6746786916953731 1103 90-91 0.6480354348011765 1103 92-93 0.6304229509290842 1103 94-95 0.6512181324276405 1103 96-97 0.6680486905596545 1103 98-99 0.690672334824626 1103 100-101 0.668858014924762 1104 1 1.2588542352607206 1104 2 0.768874206556891 1104 3 0.9133724097829159 1104 4 0.9488567180672263 1104 5 0.836250894374885 1104 6 0.6163121596322227 1104 7 0.5086669809181288 1104 8 0.5093394563769778 1104 9 0.47810587132907045 1104 10-11 0.5733014763550415 1104 12-13 0.5509892807229235 1104 14-15 0.5863709539156048 1104 16-17 0.48635946055064494 1104 18-19 0.48875057282182865 1104 20-21 0.5328715653577731 1104 22-23 0.5336047637294214 1104 24-25 0.5400160978381123 1104 26-27 0.5708288034389852 1104 28-29 0.5997117045190805 1104 30-31 0.5838930024731894 1104 32-33 0.5601607718190564 1104 34-35 0.5324866099117074 1104 36-37 0.5155134440558484 1104 38-39 0.5411015540555724 1104 40-41 0.5156503733820941 1104 42-43 0.5500849448570122 1104 44-45 0.5641732579231302 1104 46-47 0.5587924803057049 1104 48-49 0.5296699878181315 1104 50-51 0.5266279026759406 1104 52-53 0.47771110392096006 1104 54-55 0.48408796081498906 1104 56-57 0.5342235906557349 1104 58-59 0.5701018058387533 1104 60-61 0.5590678316689974 1104 62-63 0.6041175383312449 1104 64-65 0.5950336678740413 1104 66-67 0.5521324947296193 1104 68-69 0.4170887660008056 1104 70-71 0.49445862085327263 1104 72-73 0.5290565666515441 1104 74-75 0.5115353651496442 1104 76-77 0.42098542084784896 1104 78-79 0.5437457888653192 1104 80-81 0.5690620163806877 1104 82-83 0.5109996343888135 1104 84-85 0.5201047213500161 1104 86-87 0.50711029427897 1104 88-89 0.5009270736107752 1104 90-91 0.47630434265625254 1104 92-93 0.5338280600116114 1104 94-95 0.5653946394734497 1104 96-97 0.5104343156642486 1104 98-99 0.5324804958073699 1104 100-101 0.6076546996912846 1105 1 1.1327164204396922 1105 2 0.6465454795972754 1105 3 0.803841096909224 1105 4 0.8388481814000102 1105 5 0.7199703929074559 1105 6 0.4570777005785871 1105 7 0.3952873128783878 1105 8 0.3877648255944024 1105 9 0.40006329792837647 1105 10-11 0.42565173965854797 1105 12-13 0.3694336346694129 1105 14-15 0.4015687739315794 1105 16-17 0.37092441476464444 1105 18-19 0.3555572651220871 1105 20-21 0.31110109321389956 1105 22-23 0.34568546292563695 1105 24-25 0.3215615512468375 1105 26-27 0.27059878386167213 1105 28-29 0.31188349953866634 1105 30-31 0.36797826108118414 1105 32-33 0.3838566306844058 1105 34-35 0.3769921817651394 1105 36-37 0.2986121987498578 1105 38-39 0.33843337532652384 1105 40-41 0.32246462048119184 1105 42-43 0.30110084633312084 1105 44-45 0.33870612122317567 1105 46-47 0.3274363093337769 1105 48-49 0.3710188067621516 1105 50-51 0.37321231082258777 1105 52-53 0.5046311073614618 1105 54-55 0.4717031773995899 1105 56-57 0.38006429421306365 1105 58-59 0.43402844591648204 1105 60-61 0.37002886128360046 1105 62-63 0.3601252729690856 1105 64-65 0.3416101459436476 1105 66-67 0.2995663318432804 1105 68-69 0.20709453453527615 1105 70-71 0.2651530578384538 1105 72-73 0.33328384911184017 1105 74-75 0.29752790892382563 1105 76-77 0.2274335510239638 1105 78-79 0.32983854606147744 1105 80-81 0.34453159072267425 1105 82-83 0.31988777038446514 1105 84-85 0.3748286212749079 1105 86-87 0.38417360806942824 1105 88-89 0.3003899342738521 1105 90-91 0.3667530699066113 1105 92-93 0.415800158140069 1105 94-95 0.43731752763154574 1105 96-97 0.3755262420265204 1105 98-99 0.311073855050779 1105 100-101 0.3421584206323409 1106 1 0.9369186009092694 1106 2 0.42408897177044125 1106 3 0.5806434640623941 1106 4 0.6596445625356466 1106 5 0.45090217315163983 1106 6 0.2956852961883456 1106 7 0.1448063019205179 1106 8 0.0867105629714402 1106 9 0.07097820589590498 1106 10-11 0.06665766898217385 1106 12-13 0.019459058643391813 1106 14-15 0.04627898245630746 1106 16-17 0.02952848892698512 1106 18-19 -2.1301390593464475E-4 1106 20-21 0.038101919411914764 1106 22-23 0.11643155275049111 1106 24-25 0.12549943705982258 1106 26-27 0.11264299317627291 1106 28-29 0.07514851858349658 1106 30-31 0.016100479947361634 1106 32-33 0.07525380516173641 1106 34-35 0.018281602804727015 1106 36-37 0.0637750941399915 1106 38-39 0.02621553748274863 1106 40-41 0.0502738910121181 1106 42-43 0.02495687960687576 1106 44-45 0.055431866498551585 1106 46-47 0.05651150795731752 1106 48-49 0.06755563941449338 1106 50-51 0.07223002277176249 1106 52-53 0.1330571488369401 1106 54-55 0.14436570512117441 1106 56-57 0.0937315563437835 1106 58-59 0.03588423351218495 1106 60-61 0.026841156362742424 1106 62-63 0.060382317384856776 1106 64-65 0.11140177470268142 1106 66-67 0.08197874439026265 1106 68-69 -0.006151788035932526 1106 70-71 0.04963184939027343 1106 72-73 0.06705866511063263 1106 74-75 -0.0029735442991061234 1106 76-77 -0.07888668764326923 1106 78-79 0.09083476845238536 1106 80-81 0.0988956766211615 1106 82-83 -0.06156784011886529 1106 84-85 0.021711872119524855 1106 86-87 0.013746153207410572 1106 88-89 0.0781001700222923 1106 90-91 0.05331357296518746 1106 92-93 0.12366658789870044 1106 94-95 0.07955279044562147 1106 96-97 0.04671429822808548 1106 98-99 0.02922335693726552 1106 100-101 -0.061897139609921936 1107 1 0.9805652042074442 1107 2 0.4500477601802082 1107 3 0.5767540104796183 1107 4 0.7538403244923586 1107 5 0.5670436452212613 1107 6 0.12854222249912084 1107 7 0.2518019301513945 1107 8 0.23116457402971946 1107 9 0.22212084728955261 1107 10-11 0.2183643780530815 1107 12-13 0.14259520035296447 1107 14-15 0.15863934728053408 1107 16-17 0.15708953932912095 1107 18-19 0.07798694780614568 1107 20-21 0.06225019016357436 1107 22-23 0.025693707684723677 1107 24-25 0.04094190603763792 1107 26-27 0.060967615331044556 1107 28-29 0.0595440032310961 1107 30-31 0.005905154628230491 1107 32-33 -0.035181339038302895 1107 34-35 0.008637068342373766 1107 36-37 0.019075528185290125 1107 38-39 -0.048650598486304375 1107 40-41 0.03544671567480151 1107 42-43 0.01942752735696729 1107 44-45 -0.01168839398619781 1107 46-47 -0.0861618115922056 1107 48-49 -0.11779446501049051 1107 50-51 -0.052863795138755165 1107 52-53 0.10221585398581112 1107 54-55 0.08613564884146996 1107 56-57 0.07650284203907631 1107 58-59 0.10522539018202082 1107 60-61 0.08882386715666257 1107 62-63 0.11308890173648933 1107 64-65 0.0891376100864747 1107 66-67 0.04555251427888862 1107 68-69 -0.07992641598158556 1107 70-71 0.033142388962982494 1107 72-73 0.03514326011165991 1107 74-75 0.050603972632949024 1107 76-77 -0.0818682509683164 1107 78-79 0.05542401311197409 1107 80-81 0.10341538959306007 1107 82-83 0.15413486991053205 1107 84-85 0.14530520171383543 1107 86-87 0.15657963007446085 1107 88-89 0.17134359601967475 1107 90-91 0.11860470887065944 1107 92-93 0.0800053578436426 1107 94-95 0.08891473777954317 1107 96-97 0.14883709024782021 1107 98-99 0.14522572205143547 1107 100-101 0.1009312435967793 1108 1 0.9176687360046678 1108 2 0.3993404860400176 1108 3 0.594057752199781 1108 4 0.6984258993327188 1108 5 0.53534822347725 1108 6 0.32813963310312744 1108 7 0.29390164284385634 1108 8 0.23130404184080078 1108 9 0.20481470498199883 1108 10-11 0.2515818661940372 1108 12-13 0.2088608932002174 1108 14-15 0.07452605334973583 1108 16-17 0.13462951593611194 1108 18-19 0.06935425727623112 1108 20-21 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-1.2591809580688995 2315 100-101 -1.4182133322570358 2316 1 -8.69723698878201 2316 2 -5.327836726492247 2316 3 -8.302906528131086 2316 4 -10.306237671002918 2316 5 -6.563401988025902 2316 6 -4.888930407624645 2316 7 -3.8929510363355746 2316 8 -3.6036860804906503 2316 9 -3.8478525780362745 2316 10-11 -3.8100064716094195 2316 12-13 -3.817145286665614 2316 14-15 -4.210946338583533 2316 16-17 -4.244548993391689 2316 18-19 -4.230816287714873 2316 20-21 -4.062711664745201 2316 22-23 -4.102670043356703 2316 24-25 -3.9988677630849168 2316 26-27 -3.953994117560498 2316 28-29 -3.8421636819475395 2316 30-31 -3.769718391859371 2316 32-33 -3.773380349723304 2316 34-35 -3.717604077465271 2316 36-37 -3.7224559076841004 2316 38-39 -3.758829630473066 2316 40-41 -3.6580762624987173 2316 42-43 -3.6574392585484503 2316 44-45 -3.6849579847580003 2316 46-47 -3.6882937856646123 2316 48-49 -3.6651364885394813 2316 50-51 -3.6850250919270096 2316 52-53 -4.0620065233005604 2316 54-55 -3.6735991965613195 2316 56-57 -3.6088437617681635 2316 58-59 -3.6198322725783854 2316 60-61 -3.6909855107063834 2316 62-63 -3.755204381994716 2316 64-65 -3.7140131840699198 2316 66-67 -3.7134401811632323 2316 68-69 -3.6355881069278553 2316 70-71 -3.555296707733266 2316 72-73 -3.4881606552594917 2316 74-75 -3.5343758589555527 2316 76-77 -3.433970488428855 2316 78-79 -3.5660676150885884 2316 80-81 -3.650671047772647 2316 82-83 -3.6061981191160584 2316 84-85 -3.617103721267192 2316 86-87 -3.652596294554133 2316 88-89 -3.643770214154589 2316 90-91 -3.6034897814337974 2316 92-93 -3.650426783086875 2316 94-95 -3.718783101030059 2316 96-97 -3.792451563146404 2316 98-99 -3.96341103528777 2316 100-101 -4.175519892777682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1169171.0 3 280481.0 4 166523.0 5 170499.0 6 436126.0 7 416604.0 8 341100.0 9 566127.0 10 815121.0 11 658041.0 12 410378.0 13 285614.0 14 231152.0 15 204290.0 16 185815.0 17 172772.0 18 164040.0 19 159631.0 20 161397.0 21 171361.0 22 190508.0 23 217719.0 24 247957.0 25 283017.0 26 330481.0 27 395434.0 28 419812.0 29 477036.0 30 573879.0 31 705445.0 32 873070.0 33 1130178.0 34 1529302.0 35 2225960.0 36 3615620.0 37 7335628.0 38 1.1854011E7 39 4334163.0 40 15182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.70762948286552 18.515157378912907 10.716052528589477 44.0611606096321 2 13.431147255540276 16.83977270395959 59.548294670605564 10.18078536989457 3 14.834921049256868 23.959064999657546 41.22971993999 19.976294011095582 4 18.23895408403635 30.324955814696658 35.0678950125587 16.368195088708294 5 19.421406578924348 32.26961309384968 34.78523517817677 13.5237451490492 6 14.42616997097275 35.63856413993937 36.409388188748245 13.525877700339642 7 12.94265874873918 19.5057983196057 53.620866354005635 13.930676577649484 8 12.484668340616176 21.947229340999648 47.14670746226541 18.421394856118763 9 14.19035814063839 22.679675774438522 45.979961998356885 17.150004086566202 10-11 17.930132583084422 32.18999014326137 34.661366760316945 15.218510513337268 12-13 16.67358357985334 25.627020130003427 40.978903998719154 16.720492291424083 14-15 16.757719592975214 28.322736963200683 39.61201398748248 15.307529456341614 16-17 17.674350514700546 27.77216440150461 38.26272847019052 16.29075661360432 18-19 17.623880569484346 28.370156698299688 37.80657148353611 16.199391248679852 20-21 18.505228629654077 28.314610104219216 36.83040571612115 16.349755550005554 22-23 18.55097572348774 29.702392597444693 35.64512222695578 16.10150945211179 24-25 17.963111220414447 30.431989471820092 34.47012833125183 17.134770976513636 26-27 18.191782444818198 30.038917620375493 34.574355504666734 17.19494443013957 28-29 18.156034887101164 30.880559751080106 33.5134502041269 17.449955157691836 30-31 18.25241180236562 31.45408099106775 33.19063623254903 17.102870974017595 32-33 18.007751122778977 31.04434401767444 33.07918501997669 17.86871983956989 34-35 18.51548780903708 31.385126930356584 32.378309329714625 17.72107593089171 36-37 18.22089324275568 31.4586865975227 32.78131617076592 17.539103988955706 38-39 18.546608574532648 30.76169082650343 33.34242257775624 17.349278021207688 40-41 18.48621680746558 30.77105598797783 33.45982166623339 17.2829055383232 42-43 18.232539934079323 30.675389136711374 33.58099830669127 17.511072622518036 44-45 18.46968082709032 30.16887681308144 33.68985379244724 17.671588567381 46-47 18.632313238100355 30.198157543109893 33.66840876381911 17.501120454970646 48-49 18.10173213045671 31.194335517757406 33.169860660418294 17.534071691367593 50-51 18.08339572460271 30.644945343008956 33.419570713795196 17.852088218593146 52-53 18.90668338079439 30.953572348924034 32.23519799083183 17.904546279449747 54-55 18.3736563901848 31.493764255661024 31.899821625324705 18.232757728829476 56-57 18.672648112067026 31.56673045883464 32.13707281402037 17.623548615077965 58-59 18.475630257035846 31.76749607374532 31.74318738243187 18.01368628678696 60-61 18.765612363467454 31.56839719612477 31.378404540269216 18.28758590013856 62-63 18.737793245238198 31.914735670141564 31.883162925305548 17.46430815931469 64-65 18.965855784247836 31.914005793792732 31.389497430411208 17.730640991548224 66-67 18.885569595068937 32.6769647913902 30.429001159638258 18.008464453902604 68-69 18.724270461364416 32.714740132702715 31.241197755576973 17.319791650355896 70-71 18.848729143213458 32.26390769277662 30.88865264288153 17.998710521128384 72-73 18.341337173831604 32.719390082610396 30.90910306197243 18.03016968158557 74-75 18.528807194862825 31.64427088865205 31.980126991759917 17.84679492472521 76-77 18.070003246370973 32.41972024770811 31.39128559331122 18.118990912609693 78-79 18.4273442254888 32.47306199680806 30.960884091209874 18.13870968649327 80-81 18.41755130714282 31.947862095687796 31.477850567180504 18.15673602998888 82-83 18.820913435031667 31.811320186439048 31.060256553261674 18.30750982526762 84-85 18.546589802988663 31.4878360069454 31.388280671047355 18.57729351901858 86-87 18.624857647587287 32.22380785625242 31.1968917312401 17.954442764920195 88-89 18.504372574067947 31.5990580692542 31.684620547334575 18.21194880934328 90-91 18.625158688688927 32.13239220385842 31.309995128410517 17.932453979042133 92-93 18.85038741120419 31.990978311555228 31.242533238328324 17.916101038912256 94-95 18.539278316804268 31.774741680208113 31.43918782289974 18.24679218008788 96-97 18.38361130442951 32.21916430822138 31.08376216925376 18.313462218095346 98-99 18.365052470057886 32.10652044408162 31.389826334534593 18.138600751325907 100-101 18.794823606163234 32.731129483094925 30.494618884632768 17.97942802610907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14816.0 1 24301.0 2 31188.0 3 33780.5 4 45791.5 5 61710.0 6 78881.0 7 96551.5 8 114401.0 9 131071.0 10 146262.0 11 159441.5 12 171283.0 13 180542.0 14 190743.0 15 201104.5 16 207552.5 17 214871.0 18 228377.0 19 247284.5 20 278424.5 21 320316.0 22 374820.0 23 435415.5 24 532539.0 25 661506.5 26 820562.5 27 1048722.0 28 1308796.5 29 1436493.0 30 1488680.5 31 1615491.0 32 1786431.0 33 1904898.5 34 1946988.5 35 1979755.0 36 1963816.5 37 1994333.5 38 2016095.0 39 1835033.5 40 1643557.0 41 1496028.5 42 1323840.0 43 1220647.5 44 1122366.5 45 1029831.0 46 966334.0 47 923868.0 48 897438.0 49 853292.5 50 743500.0 51 611985.5 52 526669.5 53 454171.5 54 369949.5 55 280393.5 56 220313.0 57 175266.0 58 137980.5 59 114148.5 60 93170.0 61 69362.5 62 50699.5 63 39791.5 64 28443.0 65 21478.5 66 17935.5 67 15022.5 68 11447.0 69 8913.5 70 10342.0 71 15091.5 72 14945.5 73 21942.0 74 22311.5 75 10343.5 76 6912.5 77 8647.0 78 8177.0 79 4187.5 80 2744.5 81 1881.0 82 1329.5 83 850.5 84 501.0 85 303.5 86 200.5 87 136.0 88 103.5 89 71.5 90 44.5 91 30.5 92 16.5 93 9.0 94 7.5 95 5.0 96 2.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0685509058439374 2 0.0062977217206168085 3 0.0059857955182579855 4 0.010152400995021817 5 0.04547519737016613 6 0.18162529261580745 7 0.19375853883748748 8 0.2043981822215953 9 0.21429330102051097 10-11 0.22968014244781698 12-13 0.2480689889686274 14-15 0.2505541528363256 16-17 0.25183601014966883 18-19 0.2638713525268128 20-21 0.26150686083958924 22-23 0.23283469539211005 24-25 0.2500953708671628 26-27 0.25665606686787046 28-29 0.2641832787291717 30-31 0.28159764047181 32-33 0.29794644409252186 34-35 0.29185705264574324 36-37 0.28376973972035247 38-39 0.3186690450470388 40-41 0.3494916798239188 42-43 0.3391252564710741 44-45 0.37950490025818157 46-47 0.3629363821956622 48-49 0.3592274202712642 50-51 0.34722167673084037 52-53 0.3471761400589632 54-55 0.35970897057636564 56-57 0.3513165619493976 58-59 0.360922522881893 60-61 0.3780887097628006 62-63 0.3818659766950144 64-65 0.27866621721971524 66-67 0.16308048299381758 68-69 0.3961462769957044 70-71 0.054284266544810535 72-73 0.02361190278512531 74-75 0.029941500176056155 76-77 0.033459208078569884 78-79 0.03640543074902475 80-81 0.05914530626770167 82-83 0.06305235271476545 84-85 0.03166506320660819 86-87 0.009353232403576951 88-89 0.013550575133857892 90-91 0.003903631196673 92-93 0.0051422286717328495 94-95 0.013854532418638203 96-97 0.009513749171944082 98-99 5.578242304957042E-5 100-101 0.0044671475111533535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.3920645E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.913636961807278 #Duplication Level Percentage of deduplicated Percentage of total 1 80.27507141596618 18.393938435086017 2 7.616896307463911 3.4906159352991692 3 3.039557033880979 2.0894191919716953 4 1.6699123146900234 1.5305505814743385 5 1.0777223295296277 1.234726910223756 6 0.7787153091507192 1.070591993428866 7 0.555633304518964 0.8912105876545796 8 0.47705685614849713 0.8744886089542224 9 0.37745295637805115 0.7783938011346789 >10 3.176161632735737 15.028795744832973 >50 0.4735097201892968 7.619324836569577 >100 0.4058378374514876 18.68546953764189 >500 0.04550711027181842 7.120725496048926 >1k 0.027488598512905093 11.745747246972236 >5k 0.002175837565687649 3.3628878244724074 >10k+ 0.0013014355465157696 6.083113268234644 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG 173851 0.39582979712615785 TruSeq Adapter, Index 5 (100% over 49bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86481 0.19690284603060818 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 66363 0.1510975077893323 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 60915 0.13869331836998294 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 59214 0.13482042442682707 TruSeq Adapter, Index 5 (100% over 50bp) ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 58175 0.1324547943228065 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 55655 0.12671717366627927 No Hit CTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTATT 52958 0.1205765534636388 No Hit CTGAGAAGTAATCATTGCAGGAGCAGTGTAACTCGACAAAGCTCTCGAAT 50315 0.11455888227506676 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 46652 0.10621884082075753 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 45698 0.10404674157221507 No Hit GTCTTTGTTAAGTTAGGTTAGTAATAGGCAAAACAAACAAACAAATTTTA 44601 0.10154905511975063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.48808254068217805 1.1384167969300086E-5 0.0 0.0 0.0 2 0.49313255759335956 3.1875670314040245E-5 0.0 2.2768335938600173E-6 0.0 3 0.4935423876402544 3.1875670314040245E-5 0.0 2.2768335938600173E-6 0.0 4 0.49841253469752095 3.415250390790026E-5 0.0 2.2768335938600173E-6 0.0 5 0.49910241527646054 3.415250390790026E-5 0.0 2.2768335938600173E-6 0.0 6 0.5001224367265098 3.415250390790026E-5 0.0 2.2768335938600173E-6 0.0 7 0.5008692381452959 3.6429337501760276E-5 0.0 4.5536671877200345E-6 0.0 8 0.5056574191931835 3.8706171095620295E-5 0.0 4.5536671877200345E-6 0.0 9 0.5063746217752494 4.5536671877200345E-5 0.0 4.5536671877200345E-6 0.0 10-11 0.5098547619234645 5.23671726587804E-5 0.0 6.830500781580052E-6 0.0 12-13 0.5126404678255523 5.6920839846500433E-5 0.0 9.107334375440069E-6 0.0 14-15 0.5149469002561324 5.8059256643430446E-5 0.0 1.1384167969300086E-5 0.0 16-17 0.5179819194367479 6.375134062808049E-5 0.0 1.8214668750880138E-5 0.0 18-19 0.521068167373225 6.944342461273053E-5 0.0 2.0491502344740157E-5 0.0 20-21 0.5243342851636172 8.651967656668065E-5 0.0 2.504516953246019E-5 0.0 22-23 0.527478592356738 9.448859414519072E-5 2.2768335938600173E-6 2.504516953246019E-5 0.0 24-25 0.5296825672755945 9.562701094212073E-5 2.2768335938600173E-6 2.61835863293902E-5 0.0 26-27 0.5314243449748973 1.4913260039783112E-4 2.2768335938600173E-6 3.529092070483027E-5 0.0 28-29 0.5332560576011578 1.6393201875792126E-4 2.2768335938600173E-6 3.6429337501760276E-5 0.0 30-31 0.5351970582399235 2.891578664202222E-4 5.692083984650043E-6 3.6429337501760276E-5 0.0 32-33 0.5370845532892333 3.130646191557524E-4 6.830500781580052E-6 3.756775429869028E-5 0.0 34-35 0.5395321494026328 3.187567031404024E-4 6.830500781580052E-6 3.984458789255031E-5 0.0 36-37 0.5413638620288932 3.449402894697926E-4 6.830500781580052E-6 4.212142148641032E-5 0.0 38-39 0.543368614008287 3.5860129103295273E-4 6.830500781580052E-6 4.439825508027033E-5 0.0 40-41 0.5455520974247987 3.665702086114628E-4 6.830500781580052E-6 4.5536671877200345E-5 0.0 42-43 0.5477583491772491 3.790927933776929E-4 6.830500781580052E-6 4.895192226799037E-5 0.0 44-45 0.5500750273590016 3.99584295722433E-4 6.830500781580052E-6 5.122875586185039E-5 0.0 46-47 0.5525260387227919 4.052763797070831E-4 6.830500781580052E-6 5.578242304957042E-5 0.0 48-49 0.5552764537041748 4.086916300978731E-4 7.96891757851006E-6 6.033609023729046E-5 1.1384167969300086E-6 50-51 0.5584731280699543 4.1779896447331316E-4 9.107334375440069E-6 6.716659101887051E-5 2.2768335938600173E-6 52-53 0.5616834634372969 4.2462946525489325E-4 9.107334375440069E-6 8.196600937896063E-5 2.2768335938600173E-6 54-55 0.5649985331499572 4.291831324426133E-4 9.107334375440069E-6 8.196600937896063E-5 4.5536671877200345E-6 56-57 0.5686334979825547 4.325983828334033E-4 9.107334375440069E-6 8.879651016054068E-5 4.5536671877200345E-6 58-59 0.5725883579350896 4.337367996303333E-4 9.107334375440069E-6 9.221176055133071E-5 4.5536671877200345E-6 60-61 0.5769097880962358 4.360136332241933E-4 9.107334375440069E-6 9.562701094212073E-5 6.830500781580052E-6 62-63 0.5819894038441376 4.371520500211233E-4 9.107334375440069E-6 1.0018067812984077E-4 6.830500781580052E-6 64-65 0.5883201396518654 4.4170571720884334E-4 1.1384167969300086E-5 1.0131909492677077E-4 6.830500781580052E-6 66-67 0.594457344604115 4.4512096759963336E-4 1.1384167969300086E-5 1.0701117891142082E-4 9.107334375440069E-6 68-69 0.601368672978277 4.587819691627935E-4 1.1384167969300086E-5 1.0701117891142082E-4 9.107334375440069E-6 70-71 0.6093091301368638 4.6902772033516354E-4 1.1384167969300086E-5 1.0928801250528083E-4 9.107334375440069E-6 72-73 0.6181933348201056 4.701661371320936E-4 1.1384167969300086E-5 1.0928801250528083E-4 1.0245751172370079E-5 74-75 0.6285870801760767 4.7699663791367364E-4 1.1384167969300086E-5 1.1156484609914085E-4 1.1384167969300086E-5 76-77 0.6401886857535903 4.7813505471060364E-4 1.2522584766230094E-5 1.2636426445923095E-4 1.1384167969300086E-5 78-79 0.6531677756553893 4.8041188830446366E-4 1.3661001563160104E-5 1.3433318203774102E-4 1.1384167969300086E-5 80-81 0.6686343062584805 4.849655554921837E-4 1.3661001563160104E-5 1.3774843242853106E-4 1.1384167969300086E-5 82-83 0.6861010351737776 4.917960562737637E-4 1.3661001563160104E-5 1.445789332101111E-4 1.1384167969300086E-5 84-85 0.7074167512794951 4.986265570553438E-4 1.5937835157020123E-5 1.4799418360090112E-4 1.1384167969300086E-5 86-87 0.731961017421306 5.077338914307839E-4 1.5937835157020123E-5 1.4799418360090112E-4 1.1384167969300086E-5 88-89 0.7601482173132931 5.100107250246439E-4 1.5937835157020123E-5 1.5027101719476113E-4 1.2522584766230094E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 28220 0.0 48.700394 1 GTTTTTT 694955 0.0 46.286163 1 AGATCGG 47990 0.0 43.258316 1 GATCGGA 52590 0.0 40.33851 2 ATCGGAA 54975 0.0 38.572765 3 GCGTGTT 42235 0.0 37.103947 2 AGAGCAC 59465 0.0 36.178276 9 TATGCCG 41715 0.0 34.57448 46-47 TGCCGTC 40760 0.0 34.46876 48-49 CGGAAGA 63445 0.0 34.124428 5 TCGGAAG 63975 0.0 33.452114 4 CCGTCTT 42280 0.0 33.312786 50-51 ATCTCGT 41570 0.0 32.287388 40-41 CGTCTGA 49245 0.0 29.814774 16-17 CTCGTAT 46900 0.0 29.403759 42-43 TAGGCAT 20445 0.0 29.400982 9 CCAGTCA 49920 0.0 29.229008 26-27 CACACGT 50270 0.0 29.126661 12-13 AGTGATC 45790 0.0 28.85835 36-37 CGTGTTT 50210 0.0 28.68807 3 >>END_MODULE