FastQCFastQC Report
Fri 27 May 2016
SRR1233097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44473598
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3896440.8761243018835579Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1021450.2296755931462977No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT836850.18816782037738436No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT801030.1801136035811629No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT797710.17936709325834171No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT752840.16927796127491193No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT729260.16397593916282646No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT689440.1550223123391096No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT660430.1484993411146991No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT659380.14826324598248156No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC657990.1479507009979269No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT643610.1447173219490809No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT641710.14429010218602056No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT631410.14197412136521986No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT577460.12984332861937548No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA527330.1185714724497892No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT526220.11832188616715922No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT516790.11620152702733878No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG461980.10387736112558288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT734450.063.1666229
AGATCGG770500.061.3004041
CGCGTGT373400.059.9137231
GATCGGA788950.059.9035872
AAGAGCG802850.058.7552268
ATCGGAA820400.057.867663
CGGAAGA869900.054.298565
TCGGAAG873950.053.9426044
GAAGAGC959450.049.0150957
GTTTTTT6882200.046.5775761
GCGTGTT497200.044.795422
CGCCGTA582750.041.71499646-47
GGAAGAG1156150.040.7692686
GTCGCCG601500.040.66583644-45
CCGTATC616450.039.50532548-49
GGTCGCC601050.039.3249944-45
TGGTCGC602500.039.01416442-43
TCGCCGT599800.038.69164746-47
CGTATCA611700.038.68897250-51
AGACGGC76400.038.2279979
Produced by FastQC (version 0.11.5)