FastQCFastQC Report
Fri 27 May 2016
SRR1233097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44473598
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4582811.0304563170265648TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1296440.2915077840115387TruSeq Adapter, Index 9 (100% over 50bp)
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1033760.23244352750591488No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT821510.18471858292193943No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT805450.18110745166154535No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT804330.18085561685384663No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT771000.17336128279974108No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT727980.1636881279540279No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT710280.15970823858236072No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC686640.1543927253198628No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT684660.1539475173562526No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT682180.15338988313920543No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT670940.15086254096194332No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT652740.14677022533683917No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT643540.14470158227359972No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT583630.13123066858678714No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA551490.1240039090158615No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT528040.11873111772966963No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT478890.10767961701681973No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA469800.10563570772933639No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT467610.10514328073928265No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA455350.10238658900500922No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG453830.10204481319456096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT360400.062.675731
AGATCGG794650.061.266231
GATCGGA933250.053.0328372
ATCGGAA981150.050.4875143
AGAGCAC1046350.047.9121139
CGGAAGA1045150.047.8989645
TCGGAAG1053850.047.210614
GCGTGTT487500.046.7653662
GAAGAGC1141450.043.9411137
GTTTTTT5343400.041.2781451
GGAAGAG1318550.038.132856
AAGAGCA1334350.037.958948
TATGCCG873350.036.18353746-47
AGTCACG881200.035.99863428-29
TGCCGTC869850.035.85413448-49
CCGTCTT893300.034.89963550-51
CACACGT921050.034.8046412-13
CTCGTAT888550.034.55706442-43
CGTCTGA932400.034.29692516-17
ATCTCGT895000.033.96576340-41
Produced by FastQC (version 0.11.5)