##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233097_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44473598 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.535986317095368 33.0 31.0 34.0 30.0 34.0 2 32.31195726507219 34.0 31.0 34.0 30.0 34.0 3 32.12427357462735 34.0 31.0 34.0 30.0 34.0 4 35.731002875009125 37.0 35.0 37.0 35.0 37.0 5 35.84906172871374 37.0 35.0 37.0 35.0 37.0 6 35.94468668804355 37.0 35.0 37.0 35.0 37.0 7 36.00311865929984 37.0 35.0 37.0 35.0 37.0 8 36.017761369340974 37.0 35.0 37.0 35.0 37.0 9 37.787456908703454 39.0 38.0 39.0 35.0 39.0 10-11 37.681563441302856 39.0 37.0 39.0 35.0 39.0 12-13 37.67373151369493 39.0 37.0 39.0 35.0 39.0 14-15 39.09640799694236 40.0 38.5 41.0 36.0 41.0 16-17 38.96631481221735 40.0 38.0 41.0 35.5 41.0 18-19 38.72922869878889 40.0 38.0 41.0 34.5 41.0 20-21 38.546989182210986 40.0 38.0 41.0 34.0 41.0 22-23 38.31604185701368 40.0 38.0 41.0 34.0 41.0 24-25 37.94479649476527 40.0 38.0 41.0 33.5 41.0 26-27 37.43330178727612 40.0 38.0 41.0 32.5 41.0 28-29 37.04330658158128 40.0 38.0 41.0 31.5 41.0 30-31 36.80433944202131 40.0 37.5 41.0 31.0 41.0 32-33 36.748559088922825 40.0 38.0 41.0 30.0 41.0 34-35 36.656212895120376 40.0 38.0 41.0 30.0 41.0 36-37 36.557877822253104 40.0 37.5 41.0 30.0 41.0 38-39 36.44001700739392 40.0 37.0 41.0 30.0 41.0 40-41 36.30931611379857 40.0 37.0 41.0 30.0 41.0 42-43 36.09859589053262 40.0 37.0 41.0 28.5 41.0 44-45 36.05038673731772 40.0 37.0 41.0 28.5 41.0 46-47 35.915398367363935 40.0 36.5 41.0 27.5 41.0 48-49 35.78007172929881 40.0 36.0 41.0 27.0 41.0 50-51 35.632008557076944 40.0 36.0 41.0 26.5 41.0 52-53 35.46611463727311 40.0 35.5 41.0 26.0 41.0 54-55 35.223933838229144 39.0 35.0 41.0 24.5 41.0 56-57 35.230385452510504 39.0 35.0 41.0 25.0 41.0 58-59 35.11189065476555 39.0 35.0 41.0 25.0 41.0 60-61 34.89994147763804 39.0 35.0 41.0 24.0 41.0 62-63 34.63999849978407 39.0 35.0 41.0 23.0 41.0 64-65 34.2493325927891 38.0 34.0 40.0 22.0 41.0 66-67 33.89629009777891 38.0 34.0 40.0 21.0 41.0 68-69 33.56053091319484 37.0 34.0 40.0 19.5 41.0 70-71 33.0196047439202 37.0 33.5 39.0 14.0 41.0 72-73 32.525986631439174 36.0 33.0 39.0 7.0 41.0 74-75 32.112843602174934 36.0 33.0 39.0 2.0 40.0 76-77 30.978752775972836 35.0 31.0 37.0 2.0 39.0 78-79 31.345648029197008 35.0 32.0 37.0 2.0 39.0 80-81 31.115282341221864 35.0 32.0 37.0 2.0 39.0 82-83 30.77545931858268 35.0 32.0 36.0 2.0 38.5 84-85 30.422893465916566 35.0 31.5 36.0 2.0 37.0 86-87 30.10061628024789 35.0 31.0 35.5 2.0 37.0 88-89 29.797834166689192 35.0 31.0 35.0 2.0 36.5 90-91 29.51703717562946 35.0 31.0 35.0 2.0 36.0 92-93 29.24335985813426 34.0 31.0 35.0 2.0 36.0 94-95 28.951598642862223 34.0 30.0 35.0 2.0 36.0 96-97 28.62250916824854 34.0 30.0 35.0 2.0 35.0 98-99 28.23262235270463 34.0 29.5 35.0 2.0 35.0 100-101 26.495450963962934 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8863158134226659 1101 2 0.45842679954576937 1101 3 0.44105192153223527 1101 4 0.42017884461427 1101 5 0.27335967168112063 1101 6 0.15293973304216024 1101 7 0.14231947324216065 1101 8 0.1282653758113952 1101 9 0.14529401420290355 1101 10-11 0.13126248164576992 1101 12-13 0.1159834164081559 1101 14-15 0.17756999275520258 1101 16-17 0.188661426821092 1101 18-19 0.1934578882708351 1101 20-21 0.1546576287683834 1101 22-23 0.13619961658872626 1101 24-25 0.14353502082546044 1101 26-27 0.12334901019097089 1101 28-29 0.1557333108972614 1101 30-31 0.1530868320760419 1101 32-33 -0.006710793114592661 1101 34-35 0.027293807581919793 1101 36-37 0.099312679617519 1101 38-39 0.012943000836870056 1101 40-41 0.06265295676008265 1101 42-43 0.025442040610052175 1101 44-45 -0.034223135511766145 1101 46-47 -0.08388109877790839 1101 48-49 -0.054685464195365796 1101 50-51 -0.09756398182811665 1101 52-53 -0.09079989958595291 1101 54-55 -0.03508972271339417 1101 56-57 -0.1137269359582902 1101 58-59 -0.04393347912129286 1101 60-61 0.026680827660904072 1101 62-63 -0.04190915257328953 1101 64-65 -0.029855656244464512 1101 66-67 -0.16974874065790146 1101 68-69 -0.1011759158419423 1101 70-71 -0.1362217085701687 1101 72-73 -0.07284972461549444 1101 74-75 -0.13284911859854986 1101 76-77 -0.22504045479119128 1101 78-79 -0.13837150591611547 1101 80-81 -0.08117442992516999 1101 82-83 -0.06699851878747154 1101 84-85 0.040205721246554305 1101 86-87 -0.005473216467134279 1101 88-89 -0.013305449498517419 1101 90-91 0.03783879601979123 1101 92-93 0.06395750713432591 1101 94-95 0.10887535986959662 1101 96-97 0.04663540667755939 1101 98-99 0.07151548536525354 1101 100-101 0.23893810756180045 1102 1 0.8670449074437236 1102 2 0.3972660742667813 1102 3 0.42611123628102376 1102 4 0.3819629622684175 1102 5 0.21508980166443337 1102 6 0.045976882517550166 1102 7 0.1290473516684898 1102 8 0.11702160614979107 1102 9 0.12398413572374523 1102 10-11 0.0802044333698575 1102 12-13 0.12145983366088586 1102 14-15 0.15654480427465955 1102 16-17 0.15636393543932314 1102 18-19 0.15192358193864663 1102 20-21 0.17087502351783712 1102 22-23 0.0935359136643612 1102 24-25 0.05816810929099603 1102 26-27 0.04529388208431584 1102 28-29 0.08004003375511104 1102 30-31 0.09315441271281344 1102 32-33 -0.025631219684775886 1102 34-35 -0.06515956442123638 1102 36-37 -0.06532978444973736 1102 38-39 -0.06195822673027607 1102 40-41 -0.008946936009031958 1102 42-43 -0.0033703870542467484 1102 44-45 -0.01188108078165584 1102 46-47 -0.00656206070699028 1102 48-49 -0.07140832664267549 1102 50-51 -0.09949470856969356 1102 52-53 -0.038504053897739254 1102 54-55 0.008063075920034635 1102 56-57 -0.07065256793806185 1102 58-59 -0.01847843086944323 1102 60-61 0.07036945682892082 1102 62-63 0.1112032386164401 1102 64-65 0.0783946354457754 1102 66-67 0.03135179215365014 1102 68-69 0.08765775579809798 1102 70-71 -0.03913737264286965 1102 72-73 0.059792865958833374 1102 74-75 0.06871436199680403 1102 76-77 0.07265693049281552 1102 78-79 0.14659806149195376 1102 80-81 0.1747353016735822 1102 82-83 0.11863459068425186 1102 84-85 0.13430438165246628 1102 86-87 0.14215161102102414 1102 88-89 0.10623348144649825 1102 90-91 0.10016629059838067 1102 92-93 0.09078548583349288 1102 94-95 0.07729379764106525 1102 96-97 0.06457911218492995 1102 98-99 0.16273027148611519 1102 100-101 0.26808622664889725 1103 1 0.8468430601411008 1103 2 0.3922272478728601 1103 3 0.4203474547214512 1103 4 0.39029573426397235 1103 5 0.21798896122842137 1103 6 0.14949527360133175 1103 7 0.07445066752416096 1103 8 0.08931129430940388 1103 9 0.1291336761557531 1103 10-11 0.08083232528109363 1103 12-13 0.1065122113644108 1103 14-15 0.1262862872985977 1103 16-17 0.13266377544167796 1103 18-19 0.10565399439384038 1103 20-21 0.15250705885414106 1103 22-23 0.061731934737373706 1103 24-25 0.07384919340370999 1103 26-27 0.1290166084437132 1103 28-29 0.06024687553510688 1103 30-31 0.10505213272561775 1103 32-33 -0.0061599658939570645 1103 34-35 0.049003914991537556 1103 36-37 0.023245287599650055 1103 38-39 -0.08748158804986161 1103 40-41 0.02633526137332609 1103 42-43 0.011710700156861265 1103 44-45 0.0014619347592628174 1103 46-47 0.027460595029353385 1103 48-49 -0.05066919917291557 1103 50-51 -0.1532782085943012 1103 52-53 -0.15240166149672518 1103 54-55 -0.10402756689957471 1103 56-57 -0.03339174308809589 1103 58-59 0.02980324339900875 1103 60-61 0.04729251337084861 1103 62-63 0.062194155192131007 1103 64-65 0.14014360566761752 1103 66-67 0.10133236599084228 1103 68-69 0.11638290293492304 1103 70-71 0.06625589434679569 1103 72-73 0.043058611530497615 1103 74-75 -0.005390334566023114 1103 76-77 0.03422895703435813 1103 78-79 0.022786888222132262 1103 80-81 0.03188891616620282 1103 82-83 0.025282023351294924 1103 84-85 0.1618368660281284 1103 86-87 0.20353662553124607 1103 88-89 0.2574485673378213 1103 90-91 0.2472466343042541 1103 92-93 0.26703917090959806 1103 94-95 0.27722881300340063 1103 96-97 0.2610061480573975 1103 98-99 0.2563833103806239 1103 100-101 0.30800145477648044 1104 1 0.7862093830932899 1104 2 0.3276366069519483 1104 3 0.36772507677473953 1104 4 0.3307000491854808 1104 5 0.1809471366974833 1104 6 0.10807092808695273 1104 7 0.07610839753070309 1104 8 0.054076098393871064 1104 9 0.039449680627960504 1104 10-11 -0.03519897663728955 1104 12-13 -0.023005304100095714 1104 14-15 -0.012655522414341647 1104 16-17 -0.026190704982397506 1104 18-19 -0.01321455307508046 1104 20-21 0.009414143535394714 1104 22-23 -0.07392676974047419 1104 24-25 -0.12646744836552415 1104 26-27 -0.0796449400406729 1104 28-29 -0.05116775949581864 1104 30-31 -0.06826940813053994 1104 32-33 -0.12969107007781133 1104 34-35 -0.12728590402002027 1104 36-37 -0.08550191710561705 1104 38-39 -0.12627776272088198 1104 40-41 -0.13806964104249175 1104 42-43 -0.04902388878486619 1104 44-45 -0.042999513853182236 1104 46-47 -0.05710910497515442 1104 48-49 -0.11069033684399443 1104 50-51 -0.256195648006468 1104 52-53 -0.23526625882155372 1104 54-55 -0.11789757617996344 1104 56-57 -0.2240459664977834 1104 58-59 -0.11006995017161358 1104 60-61 -0.11996953216340955 1104 62-63 -0.14125699369187572 1104 64-65 -0.049243529382827944 1104 66-67 -0.02770771236193781 1104 68-69 -0.1577720977863848 1104 70-71 -0.1405567872838347 1104 72-73 -0.08868538690968109 1104 74-75 -0.02536092771135401 1104 76-77 -0.02043442406437279 1104 78-79 -0.03695165303242476 1104 80-81 -0.0024526072046633374 1104 82-83 0.07088760171480502 1104 84-85 -0.007881647272633074 1104 86-87 -0.051681064182655945 1104 88-89 -0.028560595629372187 1104 90-91 0.016935846051978842 1104 92-93 -0.005634767475459768 1104 94-95 -0.01869605404151997 1104 96-97 0.03325643678116563 1104 98-99 0.07227622948291312 1104 100-101 0.08771755789777558 1105 1 0.7256072200676194 1105 2 0.28826393479013035 1105 3 0.32662698301726323 1105 4 0.32323944242963876 1105 5 0.09544679618755225 1105 6 0.0235281829365519 1105 7 0.027183455260754386 1105 8 -0.04019591398632372 1105 9 -0.01233226699811496 1105 10-11 -0.1480508494489854 1105 12-13 -0.0647487507324982 1105 14-15 -0.1343788244389046 1105 16-17 -0.0660653798925992 1105 18-19 -0.12071244457847996 1105 20-21 -0.032899085341817624 1105 22-23 -0.0668337407691908 1105 24-25 -0.06790608749101779 1105 26-27 -0.10295738704031976 1105 28-29 -0.14763248252889838 1105 30-31 -0.1809443919077225 1105 32-33 -0.24240920011499867 1105 34-35 -0.20981420610607415 1105 36-37 -0.2046153824845831 1105 38-39 -0.25870056289862475 1105 40-41 -0.2506690719087672 1105 42-43 -0.2040025897709299 1105 44-45 -0.20778945287538164 1105 46-47 -0.18917585193815967 1105 48-49 -0.19772281256434354 1105 50-51 -0.21004160462425858 1105 52-53 -0.1649973490029737 1105 54-55 -0.35411199703861485 1105 56-57 -0.23662595231036931 1105 58-59 -0.28168667650415813 1105 60-61 -0.24191752786104814 1105 62-63 -0.2652067667673492 1105 64-65 -0.19693552518990032 1105 66-67 -0.15857624072859977 1105 68-69 -0.2162186966969415 1105 70-71 -0.3024333207258394 1105 72-73 -0.25999814739747507 1105 74-75 -0.21868742745674297 1105 76-77 -0.15602547626362195 1105 78-79 -0.09688318851160105 1105 80-81 -0.060897637965727114 1105 82-83 -0.04397675618928787 1105 84-85 -0.10135908982316622 1105 86-87 -0.10638424396102053 1105 88-89 -0.17930029617412657 1105 90-91 -0.12980406683971069 1105 92-93 -0.12310250691939473 1105 94-95 -0.05989022663499455 1105 96-97 -0.07681921606604547 1105 98-99 -0.1820624946345113 1105 100-101 -0.16107006940627855 1106 1 0.6695423326523375 1106 2 0.24115580514836665 1106 3 0.26949447873884935 1106 4 0.284240275603004 1106 5 0.1107138063021651 1106 6 -0.024308771255370232 1106 7 0.010454218754368583 1106 8 -0.05923870553426269 1106 9 -0.08820105216732799 1106 10-11 -0.13505749695761438 1106 12-13 -0.16836033022791952 1106 14-15 -0.16829094452755555 1106 16-17 -0.1735773796846658 1106 18-19 -0.18491164198007937 1106 20-21 -0.18937333537737544 1106 22-23 -0.20689259253883563 1106 24-25 -0.19406117300872694 1106 26-27 -0.171779434790146 1106 28-29 -0.22379706676576205 1106 30-31 -0.14974387215571738 1106 32-33 -0.21156145216059485 1106 34-35 -0.22075383059638654 1106 36-37 -0.18284567234156412 1106 38-39 -0.21831367796901446 1106 40-41 -0.19316334490856235 1106 42-43 -0.20126601018107948 1106 44-45 -0.24679809184154067 1106 46-47 -0.319274932951771 1106 48-49 -0.2409319278318165 1106 50-51 -0.22533903022515744 1106 52-53 -0.1812104749122625 1106 54-55 -0.2124062883979292 1106 56-57 -0.22392143642343143 1106 58-59 -0.16163271762406595 1106 60-61 -0.22104123730476744 1106 62-63 -0.32615958034811854 1106 64-65 -0.27306408563929097 1106 66-67 -0.3158888401626214 1106 68-69 -0.2578386356807485 1106 70-71 -0.18511360942311228 1106 72-73 -0.20364925247621812 1106 74-75 -0.17594729850750568 1106 76-77 -0.20060107950713046 1106 78-79 -0.05267540812893046 1106 80-81 -0.12471620112651749 1106 82-83 -0.15772418527491538 1106 84-85 -0.09591520180688207 1106 86-87 -0.07553405347882958 1106 88-89 -0.12715600019415163 1106 90-91 -0.12741456820952735 1106 92-93 -0.16824580482040474 1106 94-95 -0.16360717756237264 1106 96-97 -0.09600442824742572 1106 98-99 -0.09292395963469957 1106 100-101 -0.12380836659957595 1107 1 0.6349668763511254 1107 2 0.16771486398305058 1107 3 0.24730727189996315 1107 4 0.27181573533938064 1107 5 0.02006984837614567 1107 6 -0.034456260464430954 1107 7 -0.07376214566885864 1107 8 -0.06503071458303822 1107 9 -0.11590459537173814 1107 10-11 -0.2151106153912039 1107 12-13 -0.18804178573385144 1107 14-15 -0.23112664715389286 1107 16-17 -0.23525309764429636 1107 18-19 -0.21301352691580888 1107 20-21 -0.2012258067913777 1107 22-23 -0.22775858060867193 1107 24-25 -0.277547082749912 1107 26-27 -0.285000490569125 1107 28-29 -0.237438211475272 1107 30-31 -0.20611197776587176 1107 32-33 -0.2629081964165749 1107 34-35 -0.2587746689932686 1107 36-37 -0.21686283304560305 1107 38-39 -0.2921015600673371 1107 40-41 -0.30133107547381854 1107 42-43 -0.32605722380902336 1107 44-45 -0.32688901628138467 1107 46-47 -0.32050386456898394 1107 48-49 -0.4073331631814412 1107 50-51 -0.3439066305789851 1107 52-53 -0.26845235853295435 1107 54-55 -0.29954485028487454 1107 56-57 -0.3034150218643745 1107 58-59 -0.2534770987109525 1107 60-61 -0.2882241273339403 1107 62-63 -0.18953837264230344 1107 64-65 -0.18788614087637256 1107 66-67 -0.1831267601228106 1107 68-69 -0.17903676035586358 1107 70-71 -0.2723408119825166 1107 72-73 -0.196714378605499 1107 74-75 -0.24158014306883757 1107 76-77 -0.2063863661973535 1107 78-79 -0.08924149733061526 1107 80-81 -0.05182740931938312 1107 82-83 -0.18466557933669492 1107 84-85 -0.14474655997134178 1107 86-87 -0.20426586259903345 1107 88-89 -0.11153841583617918 1107 90-91 -0.11915176556412632 1107 92-93 -0.20462022177828132 1107 94-95 -0.16899682019060336 1107 96-97 -0.1716077576938737 1107 98-99 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2316 52-53 -0.5740846217651665 2316 54-55 -0.6520470291975329 2316 56-57 -0.7579163455662936 2316 58-59 -0.8177198188885697 2316 60-61 -0.9842637689228511 2316 62-63 -1.0710200481792498 2316 64-65 -1.0917160772035501 2316 66-67 -0.98187192428977 2316 68-69 -1.049033616817475 2316 70-71 -1.0242716746434155 2316 72-73 -0.9600314108692807 2316 74-75 -1.037982032864626 2316 76-77 -1.0611398369916607 2316 78-79 -1.0737063446114412 2316 80-81 -1.0151126675530513 2316 82-83 -0.962381252068468 2316 84-85 -1.0832491123897263 2316 86-87 -1.2741280874212428 2316 88-89 -1.2538474975842924 2316 90-91 -1.2535023983916886 2316 92-93 -1.414940538486114 2316 94-95 -1.4517263098228739 2316 96-97 -1.4406537546686238 2316 98-99 -1.5364901065050347 2316 100-101 -1.710767085901189 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7481.0 3 1373.0 4 3582.0 5 7733.0 6 19638.0 7 57725.0 8 192931.0 9 433792.0 10 671538.0 11 545570.0 12 331720.0 13 219488.0 14 177205.0 15 155743.0 16 145484.0 17 144998.0 18 150542.0 19 160567.0 20 173662.0 21 192988.0 22 214211.0 23 244146.0 24 282066.0 25 334014.0 26 409338.0 27 520511.0 28 504494.0 29 567582.0 30 698230.0 31 865593.0 32 1090490.0 33 1397645.0 34 1867353.0 35 2697461.0 36 4358994.0 37 8362516.0 38 1.2823793E7 39 3433247.0 40 8154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.296126798321236 20.68569940725176 10.32918743003317 44.688986364393834 2 14.9499125301263 16.142737090891544 58.22568032386316 10.681670055118994 3 14.41457677729265 25.89696796936077 38.00115264014992 21.687302613196657 4 18.41104468318484 30.97654702909353 32.760007409339806 17.85240087838182 5 18.784603395479717 31.609617013671798 33.77750322787016 15.828276362978322 6 15.35580503290964 36.84173922694539 33.525996704831485 14.27645903531349 7 12.90786277287482 20.57871279045154 51.37745769973458 15.135966736939071 8 12.538643713962609 22.475989012627224 46.103254339799534 18.882112933610635 9 14.365251041752908 23.56422118129502 42.184043665637304 19.88648411131476 10-11 19.69279124594944 32.161143752973906 31.452180324021086 16.693884677055575 12-13 17.844139797279276 25.68037557923692 38.56527978689739 17.910204836586416 14-15 17.174882050244733 28.544995392547285 37.456071127863325 16.824051429344664 16-17 18.4927201077817 28.05069762963635 36.233594142754086 17.22298811982786 18-19 19.045086030592802 28.868472705986143 36.120767651854926 15.965673611566125 20-21 18.911429203456848 28.389100652481503 35.037674487231726 17.661795656829923 22-23 19.105268928320125 29.83559706592662 33.967283240721834 17.091850765031424 24-25 17.373742731586503 29.672703791584393 34.521605380342734 18.43194809648637 26-27 17.40902995975275 29.072528604499237 34.6287138719921 18.88972756375592 28-29 17.573473592129872 31.279571758507146 32.43014990601839 18.71680474334458 30-31 18.224921896357476 29.850657686837028 33.4496851367861 18.474735280019395 32-33 18.757986479978523 30.757110094847732 31.385392969554655 19.099510455619082 34-35 19.684982537279758 30.621004623911922 31.233821018933526 18.460191819874794 36-37 19.153779732415625 30.260293983859814 32.64392955119125 17.941996732533312 38-39 19.513949602188696 30.630855412238066 31.348947750978006 18.50624723459523 40-41 19.736079145204307 30.24216749901818 32.5809719735291 17.440781382248407 42-43 19.284645015678738 29.89298279846843 32.42524812136855 18.39712406448428 44-45 19.119300849011587 29.868687485100708 32.2632340203282 18.748777645559507 46-47 19.10905994158602 29.74028208826279 32.93852456012217 18.212133410029026 48-49 18.529663374661073 30.80752315115139 32.06595630063482 18.59685717355272 50-51 18.111254457082605 30.16629776614881 32.232398871798054 19.49004890497054 52-53 18.783591559198786 30.251299883584863 31.60557866264834 19.35952989456801 54-55 18.34856941414994 30.70147821185954 31.49920768722153 19.45074468676899 56-57 18.828360817579902 30.380449542220532 32.220617724700396 18.57057191549917 58-59 19.11053722255618 29.679808006539073 31.96082313825834 19.248831632646407 60-61 19.70772974113765 30.822617499937827 30.221289943754943 19.248362815169575 62-63 19.360205576351163 30.484559625690732 32.17738083615362 17.977853961804485 64-65 19.975871976897395 30.592052615126846 31.09182441231762 18.340250995658145 66-67 18.905703334369303 32.95725027689462 29.551812965526196 18.58523342320988 68-69 18.97724015943122 31.44480012613326 31.66748730336592 17.9104724110696 70-71 19.207715103239455 31.772316914858113 30.558762077221637 18.461205904680796 72-73 18.231493210870862 33.203534600461154 30.17972033654664 18.385251852121343 74-75 18.541929078911043 30.86710681694789 31.744002362930026 18.846961741211043 76-77 18.527134908221278 31.869317162060963 30.593851435181836 19.009696494535927 78-79 18.502045865504293 31.88476340502066 30.5956558765495 19.01753485292555 80-81 18.785219491348553 31.087054166384288 31.044740297378233 19.082986044888926 82-83 19.101289038948458 31.427614649032893 30.57339210558138 18.897704206437265 84-85 19.178477531770646 31.282036141982484 30.79826822196846 18.74121810427841 86-87 19.52964655569356 31.15317317029308 30.354955764991175 18.962224509022185 88-89 19.241460967471085 31.465983480805846 30.36165187264588 18.93090367907719 90-91 18.96841245001135 31.388973745726624 31.209405409474627 18.4332083947874 92-93 19.055058239272658 31.769031819732685 30.36533383244594 18.81057610854872 94-95 18.946688878149136 31.462862171738703 30.630091225217615 18.96035772489455 96-97 18.483786668521272 31.65946787821229 30.299856372756135 19.556889080510302 98-99 18.661076792599232 32.091408258475106 30.593345768309092 18.65416918061657 100-101 18.54363110726925 32.31304353813532 30.15971700703147 18.983608347563962 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19864.0 1 26039.5 2 29091.0 3 30675.5 4 40111.0 5 51002.0 6 61597.0 7 71308.5 8 81658.0 9 91345.0 10 99031.0 11 105983.0 12 114674.0 13 123380.5 14 133250.5 15 145310.0 16 157707.0 17 169884.5 18 184725.0 19 204230.5 20 226016.5 21 256724.0 22 304066.5 23 361602.5 24 428955.5 25 511626.0 26 605551.5 27 730265.0 28 992676.5 29 1167251.0 30 1285298.5 31 1491293.5 32 1765409.5 33 1960294.0 34 1845765.0 35 1907746.0 36 2169151.5 37 2361494.0 38 2444171.5 39 2074676.0 40 1807565.5 41 1724878.5 42 1461624.5 43 1323088.5 44 1263191.0 45 1191294.0 46 1088614.0 47 998903.0 48 930457.0 49 853135.5 50 755168.0 51 652490.0 52 563384.5 53 486629.5 54 414227.0 55 352386.5 56 314069.5 57 275038.0 58 235738.5 59 201393.5 60 157682.0 61 120643.0 62 90180.0 63 64473.0 64 42704.5 65 32369.5 66 26918.5 67 19739.5 68 13935.5 69 9997.0 70 11690.0 71 19004.5 72 18776.0 73 28382.5 74 30968.0 75 16051.5 76 11184.5 77 12614.0 78 12029.0 79 7172.5 80 4639.0 81 2939.0 82 2153.5 83 1536.0 84 730.5 85 279.0 86 155.0 87 86.5 88 52.0 89 43.0 90 49.0 91 44.0 92 35.5 93 29.5 94 16.0 95 8.0 96 4.5 97 4.0 98 7.0 99 8.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08401164214327791 2 0.0 3 0.41366790247103463 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.486994823310675E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 4.5982337655703056E-4 96-97 0.0011006530211475133 98-99 0.002069767325773822 100-101 0.07369878191550862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.4473598E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.338793613151832 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88995614234831 15.028325680640627 2 9.650097194762647 3.925426703822659 3 4.081674530678534 2.490490076265872 4 2.3212960544077346 1.888494454624903 5 1.5050926264396296 1.5305884148916127 6 1.083256187476305 1.3219274416350164 7 0.8674123022070357 1.2349483854468448 8 0.6461386258699605 1.0513344125643724 9 0.5132503988535136 0.9395004540734527 >10 4.353250624518337 18.026043631704948 >50 0.588154907292897 8.344963822435432 >100 0.43665850324607286 17.280626823225 >500 0.037981008442856824 5.287116377723549 >1k 0.0218722407682696 8.35378445517085 >5k 0.0019989966709652893 2.806251731575953 >10k+ 0.0019096560168511835 10.490177134198705 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG 458281 1.0304563170265648 TruSeq Adapter, Index 9 (100% over 49bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC 129644 0.2915077840115387 TruSeq Adapter, Index 9 (100% over 50bp) CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 103376 0.23244352750591488 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 82151 0.18471858292193943 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 80545 0.18110745166154535 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 80433 0.18085561685384663 No Hit CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT 77100 0.17336128279974108 No Hit ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT 72798 0.1636881279540279 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 71028 0.15970823858236072 No Hit CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC 68664 0.1543927253198628 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68466 0.1539475173562526 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 68218 0.15338988313920543 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 67094 0.15086254096194332 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 65274 0.14677022533683917 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 64354 0.14470158227359972 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 58363 0.13123066858678714 No Hit ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA 55149 0.1240039090158615 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 52804 0.11873111772966963 No Hit CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT 47889 0.10767961701681973 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 46980 0.10563570772933639 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 46761 0.10514328073928265 No Hit ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA 45535 0.10238658900500922 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 45383 0.10204481319456096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1398538071959008 0.0 0.0 0.0 0.0 2 1.1520183278177762 0.0 0.0 0.0 0.0 3 1.152389334454118 0.0 0.0 0.0 0.0 4 1.1565468573062156 0.0 0.0 0.0 0.0 5 1.157153959074775 0.0 0.0 0.0 0.0 6 1.1580713573028205 0.0 0.0 0.0 0.0 7 1.1588898204278413 0.0 0.0 0.0 0.0 8 1.164610068202712 0.0 0.0 0.0 0.0 9 1.1651407201189343 0.0 0.0 0.0 0.0 10-11 1.1673139195978703 0.0 0.0 0.0 0.0 12-13 1.1696625040321678 0.0 0.0 0.0 0.0 14-15 1.1717120346323227 0.0 0.0 0.0 0.0 16-17 1.1737593167074092 0.0 0.0 0.0 0.0 18-19 1.1757031666293336 0.0 0.0 0.0 0.0 20-21 1.188233072574879 0.0 0.0 0.0 0.0 22-23 1.2007697240956308 0.0 1.1242625343692678E-6 0.0 0.0 24-25 1.2025348162745906 0.0 2.2485250687385357E-6 0.0 0.0 26-27 1.204307778291291 0.0 2.2485250687385357E-6 0.0 0.0 28-29 1.2059615684793483 0.0 2.2485250687385357E-6 0.0 0.0 30-31 1.207556897015618 0.0 4.497050137477071E-6 2.2485250687385357E-6 0.0 32-33 1.2095884394152234 0.0 4.497050137477071E-6 5.621312671846339E-6 0.0 34-35 1.2117503962688154 0.0 4.497050137477071E-6 8.994100274954143E-6 0.0 36-37 1.2137988026064364 0.0 4.497050137477071E-6 8.994100274954143E-6 0.0 38-39 1.2159247830589286 0.0 4.497050137477071E-6 8.994100274954143E-6 0.0 40-41 1.2184791075370156 0.0 4.497050137477071E-6 8.994100274954143E-6 0.0 42-43 1.22213408503625 0.0 4.497050137477071E-6 8.994100274954143E-6 0.0 44-45 1.225020066961976 0.0 4.497050137477071E-6 8.994100274954143E-6 0.0 46-47 1.2278711967491365 0.0 4.497050137477071E-6 1.1242625343692678E-5 0.0 48-49 1.2309258180550178 0.0 4.497050137477071E-6 1.3491150412431213E-5 0.0 50-51 1.235228370774049 0.0 4.497050137477071E-6 1.3491150412431213E-5 0.0 52-53 1.24224039620091 0.0 4.497050137477071E-6 1.4615412946800481E-5 0.0 54-55 1.246974665733139 0.0 4.497050137477071E-6 1.573967548116975E-5 0.0 56-57 1.251495325383838 0.0 4.497050137477071E-6 1.573967548116975E-5 0.0 58-59 1.2561655119516077 0.0 4.497050137477071E-6 1.573967548116975E-5 0.0 60-61 1.2614596642259528 2.2485250687385357E-6 4.497050137477071E-6 1.573967548116975E-5 0.0 62-63 1.2677364219553362 2.2485250687385357E-6 4.497050137477071E-6 1.686393801553902E-5 0.0 64-65 1.275237501584648 2.2485250687385357E-6 4.497050137477071E-6 1.7988200549908285E-5 0.0 66-67 1.2832152685285323 2.2485250687385357E-6 4.497050137477071E-6 1.7988200549908285E-5 0.0 68-69 1.2920013802346282 2.2485250687385357E-6 4.497050137477071E-6 1.7988200549908285E-5 0.0 70-71 1.3015441206263545 3.3727876031078037E-6 4.497050137477071E-6 1.7988200549908285E-5 0.0 72-73 1.314945330036036 4.497050137477071E-6 4.497050137477071E-6 1.7988200549908285E-5 0.0 74-75 1.3285567765396449 4.497050137477071E-6 4.497050137477071E-6 2.023672561864682E-5 0.0 76-77 1.342538105417061 4.497050137477071E-6 4.497050137477071E-6 2.2485250687385356E-5 0.0 78-79 1.3593492030934846 4.497050137477071E-6 4.497050137477071E-6 2.585803829049316E-5 0.0 80-81 1.377738765368163 4.497050137477071E-6 4.497050137477071E-6 2.9230825893600962E-5 0.0 82-83 1.400548927927981 4.497050137477071E-6 4.497050137477071E-6 3.14793509623395E-5 0.0 84-85 1.4276166727054553 4.497050137477071E-6 6.745575206215607E-6 3.372787603107803E-5 0.0 86-87 1.4587036560433002 4.497050137477071E-6 7.869837740584875E-6 3.372787603107803E-5 2.2485250687385357E-6 88-89 1.4951814782334454 4.497050137477071E-6 8.994100274954143E-6 3.372787603107803E-5 2.2485250687385357E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 36040 0.0 62.67573 1 AGATCGG 79465 0.0 61.26623 1 GATCGGA 93325 0.0 53.032837 2 ATCGGAA 98115 0.0 50.487514 3 AGAGCAC 104635 0.0 47.912113 9 CGGAAGA 104515 0.0 47.898964 5 TCGGAAG 105385 0.0 47.21061 4 GCGTGTT 48750 0.0 46.765366 2 GAAGAGC 114145 0.0 43.941113 7 GTTTTTT 534340 0.0 41.278145 1 GGAAGAG 131855 0.0 38.13285 6 AAGAGCA 133435 0.0 37.95894 8 TATGCCG 87335 0.0 36.183537 46-47 AGTCACG 88120 0.0 35.998634 28-29 TGCCGTC 86985 0.0 35.854134 48-49 CCGTCTT 89330 0.0 34.899635 50-51 CACACGT 92105 0.0 34.80464 12-13 CTCGTAT 88855 0.0 34.557064 42-43 CGTCTGA 93240 0.0 34.296925 16-17 ATCTCGT 89500 0.0 33.965763 40-41 >>END_MODULE