FastQCFastQC Report
Fri 27 May 2016
SRR1233096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58254398
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3336390.5727275732898313Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1303340.22373246394203575No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT999010.17149091472887593No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT800390.13739563491841422No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA746950.12822207861456228No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT659820.11326526797169889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT453050.063.461471
AGATCGG659600.058.1352921
GATCGGA687550.055.568962
AGAGCGT683500.055.4836739
ATCGGAA714950.053.4316143
GTTTTTT10465300.049.25481
TCGGAAG788950.047.7891924
GCGTGTT668950.045.3391882
CGGAAGA836450.045.069765
AAGAGCG903100.042.3437658
CGCCGTA493600.042.01664446-47
GAAGAGC938800.040.2170527
CCGTATC523500.039.77456748-49
GTCGCCG520350.039.38069544-45
GCCGTAT527750.036.75826348-49
GGTCGCC524700.036.6597244-45
TGGTCGC550350.036.5036142-43
TCTCGGT566850.036.45387336-37
TCGCCGT524400.036.219946-47
TCGGTGG577150.036.12162838-39
Produced by FastQC (version 0.11.5)