##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233096_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58254398 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45717567968001 34.0 31.0 34.0 31.0 34.0 2 32.623955121809 34.0 31.0 34.0 31.0 34.0 3 32.63390959769252 34.0 31.0 34.0 31.0 34.0 4 35.96193314022402 37.0 37.0 37.0 35.0 37.0 5 35.970487017993044 37.0 37.0 37.0 35.0 37.0 6 35.931479319381175 37.0 37.0 37.0 35.0 37.0 7 35.85706110978951 37.0 37.0 37.0 35.0 37.0 8 35.756064065755176 37.0 37.0 37.0 35.0 37.0 9 37.5088091717985 39.0 38.0 39.0 35.0 39.0 10-11 37.586739390904015 39.0 38.0 39.0 35.0 39.0 12-13 37.61270460644019 39.0 38.0 39.0 35.0 39.0 14-15 39.067185176302054 41.0 39.0 41.0 36.0 41.0 16-17 38.84267759491738 41.0 39.0 41.0 36.0 41.0 18-19 38.33031912886646 41.0 38.0 41.0 34.5 41.0 20-21 37.92882060166513 40.0 38.0 41.0 34.0 41.0 22-23 37.42630922904739 40.0 38.0 41.0 32.5 41.0 24-25 36.909507613485246 41.0 38.0 41.0 31.5 41.0 26-27 36.40051200082782 41.0 38.0 41.0 28.5 41.0 28-29 35.916202713141075 40.0 38.0 41.0 23.5 41.0 30-31 35.55605967295379 40.0 38.0 41.0 17.0 41.0 32-33 35.32177547350159 40.0 37.0 41.0 13.0 41.0 34-35 35.18590455608175 40.0 37.0 41.0 9.5 41.0 36-37 35.0542645466871 40.0 37.0 41.0 8.0 41.0 38-39 34.95636471086698 40.0 37.0 41.0 7.0 41.0 40-41 34.831676768851 40.0 37.0 41.0 4.0 41.0 42-43 34.68681191246711 40.0 37.0 41.0 2.0 41.0 44-45 34.55776654837288 40.0 36.0 41.0 2.0 41.0 46-47 34.44556251358052 40.0 36.0 41.0 2.0 41.0 48-49 34.467280461811654 40.0 36.0 41.0 2.0 41.0 50-51 33.83313570590842 39.5 35.0 40.5 2.0 41.0 52-53 33.855084778320084 39.5 35.0 40.5 2.0 41.0 54-55 34.167549306749336 40.0 36.0 41.0 2.0 41.0 56-57 34.08765949310814 40.0 35.0 41.0 2.0 41.0 58-59 33.931280553272565 40.0 35.0 41.0 2.0 41.0 60-61 33.74010527067845 39.5 35.0 41.0 2.0 41.0 62-63 33.502688063139885 39.0 35.0 41.0 2.0 41.0 64-65 33.18450929661998 39.0 34.5 41.0 2.0 41.0 66-67 32.76530178888811 38.5 34.0 41.0 2.0 41.0 68-69 32.42355317104126 37.5 34.0 40.0 2.0 41.0 70-71 32.08145267418264 37.0 34.0 40.0 2.0 41.0 72-73 31.695105930027808 36.5 33.5 39.0 2.0 41.0 74-75 31.311895944749097 36.0 33.0 39.0 2.0 41.0 76-77 30.94020088406029 35.5 33.0 38.5 2.0 40.0 78-79 30.54270441520999 35.0 32.0 37.0 2.0 39.0 80-81 30.177005777314875 35.0 32.0 37.0 2.0 39.0 82-83 29.83513386405607 35.0 31.5 36.5 2.0 39.0 84-85 29.49549735283506 35.0 31.0 36.0 2.0 37.0 86-87 29.137085907230556 35.0 31.0 36.0 2.0 37.0 88-89 28.860467213136424 35.0 31.0 35.0 2.0 37.0 90-91 28.585906475250162 35.0 30.0 35.0 2.0 36.0 92-93 28.40238781628127 35.0 30.0 35.0 2.0 36.0 94-95 28.14853067402739 35.0 30.0 35.0 2.0 36.0 96-97 27.862652146881683 35.0 29.0 35.0 2.0 36.0 98-99 27.54118677872184 34.0 29.0 35.0 2.0 35.0 100-101 25.742086691892347 32.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14244467930812021 1101 2 0.09126167180821909 1101 3 0.20430944421877228 1101 4 0.16008719757229528 1101 5 0.2368283330800125 1101 6 0.28301157634485463 1101 7 0.16997514383145074 1101 8 0.15761854221816662 1101 9 0.21863093865346883 1101 10-11 0.22200116002010617 1101 12-13 0.2845690441474531 1101 14-15 0.33384647084636043 1101 16-17 0.3339279311968326 1101 18-19 0.3526607221327964 1101 20-21 0.37018857358413015 1101 22-23 0.3417460248463655 1101 24-25 0.36964413507128313 1101 26-27 0.2861827912832524 1101 28-29 0.31932639858136724 1101 30-31 0.2352136137662839 1101 32-33 0.25051042150614933 1101 34-35 0.2365959424444668 1101 36-37 0.26755529197846073 1101 38-39 0.22100876775125755 1101 40-41 0.21683396251526688 1101 42-43 0.27329842932089576 1101 44-45 0.24333789298589892 1101 46-47 0.21306421291478017 1101 48-49 0.30279782906892905 1101 50-51 0.338292279622209 1101 52-53 0.27202748261632337 1101 54-55 0.3118650175150677 1101 56-57 0.3322972415494192 1101 58-59 0.2127125340737379 1101 60-61 0.3020437147659436 1101 62-63 0.31342019079890804 1101 64-65 0.2794435091144791 1101 66-67 0.2647887636639652 1101 68-69 0.27679461064018085 1101 70-71 0.2752618866711458 1101 72-73 0.26155298835432106 1101 74-75 0.2717745872318602 1101 76-77 0.2468781886614444 1101 78-79 0.2370730062696076 1101 80-81 0.17145580399213145 1101 82-83 0.1820297388668557 1101 84-85 0.2266819460574787 1101 86-87 0.22143680994230763 1101 88-89 0.26376655685286465 1101 90-91 0.21727169437166083 1101 92-93 0.15270858110039853 1101 94-95 0.1706168868839768 1101 96-97 0.09675604333286358 1101 98-99 0.10357145164037718 1101 100-101 0.2249416884419695 1102 1 0.12000932339313408 1102 2 0.05063410990064199 1102 3 0.15483929560684118 1102 4 0.16688386414013934 1102 5 0.18486119653491073 1102 6 0.21080094309417063 1102 7 0.08684280649284659 1102 8 0.04140643322624271 1102 9 0.15612690296090648 1102 10-11 0.22070252157096348 1102 12-13 0.24206817964887506 1102 14-15 0.3161088196009274 1102 16-17 0.3227336802147178 1102 18-19 0.2930671909065836 1102 20-21 0.35786905041478434 1102 22-23 0.3509087939177462 1102 24-25 0.28593927675522934 1102 26-27 0.27414129272205656 1102 28-29 0.26645891082144857 1102 30-31 0.17939855717060738 1102 32-33 0.265761194981593 1102 34-35 0.289485497821353 1102 36-37 0.2885504611384704 1102 38-39 0.2011928564295573 1102 40-41 0.22833029337150634 1102 42-43 0.20476009872647438 1102 44-45 0.24256096702855956 1102 46-47 0.29728484257690013 1102 48-49 0.33623766272375377 1102 50-51 0.3625528051721574 1102 52-53 0.33396928854860874 1102 54-55 0.3082238341374861 1102 56-57 0.3473781423576199 1102 58-59 0.32813398295836294 1102 60-61 0.31860109555799454 1102 62-63 0.3440025029896674 1102 64-65 0.3271498112049329 1102 66-67 0.2929751027637195 1102 68-69 0.2925791983645851 1102 70-71 0.3259408528856582 1102 72-73 0.2785658297863982 1102 74-75 0.2434705912715991 1102 76-77 0.22291026173591533 1102 78-79 0.21129572182742962 1102 80-81 0.26603408560697517 1102 82-83 0.20735761365139282 1102 84-85 0.2247131939584257 1102 86-87 0.25098767536631783 1102 88-89 0.27302091786094707 1102 90-91 0.1241619815945576 1102 92-93 0.13253199422837625 1102 94-95 0.1271414195143734 1102 96-97 0.13142186565172054 1102 98-99 0.141166941911532 1102 100-101 0.19080783993327444 1103 1 -0.006186909828279852 1103 2 0.005251346622884512 1103 3 0.06701435727325844 1103 4 0.1554927247373712 1103 5 0.14873446508114085 1103 6 0.16630314243925426 1103 7 0.07271035270031234 1103 8 0.026105097964489232 1103 9 0.01585065642910166 1103 10-11 0.11538295926046516 1103 12-13 0.19856746675417725 1103 14-15 0.2540206062035182 1103 16-17 0.224476322311709 1103 18-19 0.25526446482039233 1103 20-21 0.20309970114866616 1103 22-23 0.25282804030897665 1103 24-25 0.24941701658863735 1103 26-27 0.2600656674548887 1103 28-29 0.32347811284219574 1103 30-31 0.252250171387729 1103 32-33 0.28403553228272926 1103 34-35 0.28579472914241677 1103 36-37 0.30473112380579437 1103 38-39 0.3288471084001472 1103 40-41 0.26983379173940847 1103 42-43 0.26477312787303475 1103 44-45 0.2623172934028517 1103 46-47 0.23208785474072613 1103 48-49 0.327317958855339 1103 50-51 0.34294188309013407 1103 52-53 0.3333045445778424 1103 54-55 0.28555258857151244 1103 56-57 0.2948236239039872 1103 58-59 0.3529115831912719 1103 60-61 0.32403306973078827 1103 62-63 0.32069707447922724 1103 64-65 0.2826261310430098 1103 66-67 0.28189950892112847 1103 68-69 0.31007323538933207 1103 70-71 0.28033097466508394 1103 72-73 0.2788667074994855 1103 74-75 0.278815012594535 1103 76-77 0.28027244992597034 1103 78-79 0.25490867383465243 1103 80-81 0.32111177041301886 1103 82-83 0.27544581742246876 1103 84-85 0.2801973830458344 1103 86-87 0.239207807436987 1103 88-89 0.2554654303604238 1103 90-91 0.25111470977420325 1103 92-93 0.2500217375273124 1103 94-95 0.20398669017654214 1103 96-97 0.21950576723820703 1103 98-99 0.22411566001655459 1103 100-101 0.30513638101932017 1104 1 0.053571484824999516 1104 2 0.040876486571633563 1104 3 0.08009185514215034 1104 4 0.1399113317079923 1104 5 0.13808898390021085 1104 6 0.10943392783522654 1104 7 -0.02683976379731945 1104 8 0.06282106105984298 1104 9 0.10639649073596047 1104 10-11 0.1465318307538368 1104 12-13 0.18474461244554874 1104 14-15 0.20289909911699766 1104 16-17 0.15192527243490161 1104 18-19 0.18029361905681185 1104 20-21 0.20415889461617098 1104 22-23 0.21499696084586617 1104 24-25 0.19490987030343376 1104 26-27 0.13983514832828092 1104 28-29 0.18540555782771406 1104 30-31 0.20803690776012473 1104 32-33 0.1891473087876605 1104 34-35 0.12242695971045947 1104 36-37 0.15290559898670608 1104 38-39 0.13607688444268717 1104 40-41 0.1408396173704034 1104 42-43 0.13496004930284045 1104 44-45 0.10545949612326666 1104 46-47 0.04298368528299079 1104 48-49 0.17299149388048107 1104 50-51 0.16287672353425364 1104 52-53 0.07351679873880812 1104 54-55 0.1581665054375705 1104 56-57 0.1639993730831648 1104 58-59 0.1653494584906383 1104 60-61 0.11748896874625814 1104 62-63 0.08027103507706812 1104 64-65 0.16649430327648673 1104 66-67 0.06714657844520389 1104 68-69 0.15570948848097288 1104 70-71 0.182611489901646 1104 72-73 0.1178822807376676 1104 74-75 0.08749971027907577 1104 76-77 0.0954178541322186 1104 78-79 0.13553591376035357 1104 80-81 0.1362137478668437 1104 82-83 0.1451229215671006 1104 84-85 0.07688071205877378 1104 86-87 0.14908557594408123 1104 88-89 0.06273700478602251 1104 90-91 0.016152893238459143 1104 92-93 0.08477749764875497 1104 94-95 0.08169591093548689 1104 96-97 0.11381650318987724 1104 98-99 0.06317288478625827 1104 100-101 0.049371194344860925 1105 1 0.017351075487930245 1105 2 -0.06745969157218212 1105 3 0.027471547869879487 1105 4 0.0808982374683822 1105 5 0.08990238862779165 1105 6 0.031137097996520424 1105 7 0.027101079907154713 1105 8 -0.015358879488267974 1105 9 0.0695262860594994 1105 10-11 0.007726689883696736 1105 12-13 0.03725670307459694 1105 14-15 0.08279378918768998 1105 16-17 0.09950630560754092 1105 18-19 0.04556945708662852 1105 20-21 0.07786876204139759 1105 22-23 0.05827732735004787 1105 24-25 0.09255463251292184 1105 26-27 0.0010721214357900521 1105 28-29 0.10839214019784293 1105 30-31 0.07974922223601055 1105 32-33 0.10211343958811625 1105 34-35 0.041081126753304886 1105 36-37 0.1454178729957718 1105 38-39 0.08998782727036314 1105 40-41 0.010844641030551827 1105 42-43 0.0881413519839711 1105 44-45 0.0013971797927538887 1105 46-47 -0.002105216714682001 1105 48-49 0.05118828133044673 1105 50-51 0.12006846366571011 1105 52-53 -1.3925740519482588E-4 1105 54-55 0.08468401766329947 1105 56-57 0.12319493030090456 1105 58-59 0.1446264569601965 1105 60-61 0.09429827119963363 1105 62-63 0.1654605849190247 1105 64-65 0.19593000564359642 1105 66-67 0.17034709095210587 1105 68-69 0.09328865568067357 1105 70-71 0.1477348417105162 1105 72-73 0.13632792622571088 1105 74-75 0.08920834174537617 1105 76-77 0.09550115324461572 1105 78-79 0.05226525578001073 1105 80-81 0.07522743410453359 1105 82-83 0.047642993399872324 1105 84-85 -0.045846787660874355 1105 86-87 0.015946901843612693 1105 88-89 0.014392489106512585 1105 90-91 0.03701309152925347 1105 92-93 0.04740437970149358 1105 94-95 -0.04898249825616219 1105 96-97 -0.00722140251989245 1105 98-99 0.011872093711840392 1105 100-101 -0.004703632491001031 1106 1 -0.024462163028367456 1106 2 -0.07980905147645245 1106 3 -0.04790318978675856 1106 4 0.05353856039051408 1106 5 0.06305678801884085 1106 6 0.061808238052236675 1106 7 0.06311643677160106 1106 8 0.07698932576453643 1106 9 -0.1351691366652119 1106 10-11 -0.022869894565182847 1106 12-13 0.027670386518245493 1106 14-15 0.016024807474529723 1106 16-17 0.00636307122591262 1106 18-19 0.032880545137459194 1106 20-21 0.09398172736288757 1106 22-23 0.02496751677410458 1106 24-25 0.11974191592467065 1106 26-27 0.10415976251848491 1106 28-29 0.051092628191945266 1106 30-31 0.08268129727511564 1106 32-33 -0.06731440689901547 1106 34-35 -0.0022245883946183653 1106 36-37 0.04778170496718559 1106 38-39 0.09038953232530389 1106 40-41 0.08803608648898376 1106 42-43 0.11207000475393869 1106 44-45 0.11584379168754566 1106 46-47 0.08603681340240144 1106 48-49 0.10691086648580495 1106 50-51 0.1574735554913289 1106 52-53 0.03277456286583913 1106 54-55 0.10888603233143357 1106 56-57 0.13926150819632 1106 58-59 0.18034765080319204 1106 60-61 0.16651644202387672 1106 62-63 0.2086540615933643 1106 64-65 0.2573238882237021 1106 66-67 0.25005541564362943 1106 68-69 0.22445221149074968 1106 70-71 0.20074912493211627 1106 72-73 0.2358375227300904 1106 74-75 0.19527473040654542 1106 76-77 0.15901402950872523 1106 78-79 0.18724302714678132 1106 80-81 0.1783889907231675 1106 82-83 0.20382567089786718 1106 84-85 0.17450156115136295 1106 86-87 0.19615735739831308 1106 88-89 0.15206583015854136 1106 90-91 0.12888190122374255 1106 92-93 0.15591545691086495 1106 94-95 0.152507053619221 1106 96-97 0.12376387144416512 1106 98-99 0.02154953213808497 1106 100-101 -0.08327878571688885 1107 1 -0.061268918626673496 1107 2 -0.14837540239830815 1107 3 -0.07403819525679722 1107 4 -0.035688094386273406 1107 5 -0.08315677491570028 1107 6 0.005989884655413391 1107 7 -0.001864377837719644 1107 8 0.050726863069293415 1107 9 -0.026010658039986367 1107 10-11 -0.012711082004223329 1107 12-13 -0.0209327234638792 1107 14-15 -0.10028144112058612 1107 16-17 -0.0964810562938041 1107 18-19 -0.11499153409850749 1107 20-21 -0.10567591977285673 1107 22-23 -0.028391260453254574 1107 24-25 -0.06624515097446704 1107 26-27 -0.04961507050453662 1107 28-29 -0.028966183600296347 1107 30-31 -0.08445382473288277 1107 32-33 -0.03867082150945578 1107 34-35 -0.012522049000345703 1107 36-37 -0.08283071228265726 1107 38-39 -6.647353979047921E-4 1107 40-41 -0.04263259249707119 1107 42-43 0.0039762320589602496 1107 44-45 0.015099820001452713 1107 46-47 -0.02229639877459988 1107 48-49 0.0075609351749861275 1107 50-51 0.07103335022226531 1107 52-53 0.02173684464239045 1107 54-55 0.019721566076519537 1107 56-57 0.09812580632421941 1107 58-59 0.01280294441900054 1107 60-61 0.016932478829517095 1107 62-63 -0.017846586482889393 1107 64-65 0.0556056604587809 1107 66-67 0.07257057818819135 1107 68-69 0.048350620482459306 1107 70-71 0.04963171371684183 1107 72-73 0.030612430513027533 1107 74-75 -0.017184023646915847 1107 76-77 0.0517735468364684 1107 78-79 0.053148443866543715 1107 80-81 0.11557252011859731 1107 82-83 0.08365380596352878 1107 84-85 0.11604551278094988 1107 86-87 0.09163557733266003 1107 88-89 -0.01256724449727642 1107 90-91 -9.684179848434837E-4 1107 92-93 0.030801851007222325 1107 94-95 0.03126138653441046 1107 96-97 0.02547665780836894 1107 98-99 0.018456040571386723 1107 100-101 -0.051344753754342776 1108 1 -0.14722887995176848 1108 2 -0.19771809723453515 1108 3 -0.16767873431608393 1108 4 -0.13227590388822108 1108 5 -0.12459641332742422 1108 6 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2316 72-73 -1.175085428855489 2316 74-75 -1.161734888318943 2316 76-77 -1.1464585448457747 2316 78-79 -1.1593525081150453 2316 80-81 -1.1528388114761228 2316 82-83 -1.2255763560182196 2316 84-85 -1.2426931540211257 2316 86-87 -1.2464126478636715 2316 88-89 -1.3389236197911423 2316 90-91 -1.2501666458102179 2316 92-93 -1.3571976992613948 2316 94-95 -1.3306404579022626 2316 96-97 -1.368300500366363 2316 98-99 -1.4735725151190984 2316 100-101 -1.9651095265971747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 154807.0 3 69153.0 4 47766.0 5 60349.0 6 110920.0 7 244352.0 8 553616.0 9 1146476.0 10 1429002.0 11 1087019.0 12 657394.0 13 449919.0 14 341778.0 15 266728.0 16 222495.0 17 210141.0 18 205898.0 19 209076.0 20 224154.0 21 246374.0 22 275636.0 23 313544.0 24 378827.0 25 399011.0 26 333657.0 27 378581.0 28 435148.0 29 511385.0 30 610828.0 31 743718.0 32 931429.0 33 1201507.0 34 1641296.0 35 2475548.0 36 4398435.0 37 1.0051462E7 38 1.8667896E7 39 6550416.0 40 18657.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.514840550784534 17.81593299887477 8.779957001127805 42.88926944921289 2 14.512407339030512 15.153493233631751 59.56536513436363 10.768734292974104 3 15.88365319978988 22.518700857743422 39.92920873010956 21.66843721235714 4 19.116976126205277 27.76090822448164 35.82041592591203 17.301699723401054 5 19.256529022131744 29.95481166916515 37.3162338866507 13.472425422052403 6 15.121959925475215 34.65797126085022 37.05921478809659 13.160854025577976 7 12.653192843655312 19.28241784778598 54.2111779842406 13.853211324318112 8 12.173829707659591 21.16455453709728 49.360819498897825 17.300796256345304 9 14.145222300821333 22.721979279515477 46.04971105207933 17.083087367583857 10-11 17.28400126211673 31.515937169202846 35.8345297745704 15.365531794110025 12-13 16.394882297702974 25.082023725304627 42.305448557466526 16.217645419525866 14-15 16.542899831942005 27.113251489690054 41.104284308078114 15.239564370289823 16-17 17.94727131020708 26.88174910158588 39.43788452165719 15.733095066549858 18-19 17.70775639551026 28.29611204493997 38.78651753073328 15.20961402881649 20-21 18.26336506808037 28.334808096544496 37.70461931631903 15.6972075190561 22-23 19.159916937776593 28.626330869103654 36.47083027269188 15.742921920427873 24-25 18.18780125789714 29.806032009381276 35.54793345488492 16.45823327783667 26-27 17.91794948814015 30.418459616647187 34.804039544474826 16.859551350737842 28-29 18.179519782448242 31.02640623250631 33.82264251369641 16.971431471349035 30-31 17.998061511283428 31.102528490915194 33.71985241953241 17.179557578268966 32-33 18.49661490539227 30.748785711594472 33.29828572013565 17.456313662877605 34-35 18.792723245229695 31.143507262306446 32.77425017337218 17.28951931909168 36-37 18.127460824038643 30.880878971162524 33.740185909380656 17.25147429541818 38-39 18.60387161105543 30.21303353247422 33.81239477319934 17.370700083271018 40-41 18.666737187014387 30.08432131604711 34.02040627879714 17.228535218141364 42-43 18.991692404981425 30.21556790132609 33.812515517313905 16.98022417637858 44-45 19.19966361747541 30.022396373370754 33.725924250915234 17.052015758238603 46-47 18.131753249692007 29.884628893490483 34.11547667160804 17.868141185209463 48-49 18.181445058014184 30.768207685313616 32.96690436058638 18.08344289608582 50-51 17.83146902939326 30.909116590910752 33.24931907657927 18.010095303116717 52-53 17.713501542095685 31.371248941841205 32.791399808455786 18.123849707607327 54-55 17.911188071596122 31.693433767098544 31.78696718951968 18.608410971785656 56-57 18.184366957428647 31.987224367120344 32.14325592782236 17.685152747628656 58-59 18.193033686890818 31.63734887723454 32.134965994278964 18.03465144159568 60-61 18.715508999195666 32.517864765456665 30.975036874821924 17.791589360525744 62-63 18.530659544193885 32.64891815133519 31.437057028897726 17.383365275573205 64-65 18.956296321449187 32.0469023167753 31.462291963756556 17.53450939801895 66-67 18.65465680967765 32.80601239621412 30.45998933702413 18.0793414570841 68-69 18.594495612644742 32.31339232911404 31.7463330490803 17.345779009160918 70-71 18.68402674997301 31.897090662782595 31.499914676270908 17.918967910973485 72-73 17.9161410991841 32.85232187554119 30.96145348517701 18.27008354009769 74-75 18.163058443567174 31.46497377101543 32.217004675635216 18.154963109782177 76-77 18.113148889035553 32.045219570292346 31.518180789522276 18.323450751149824 78-79 18.230385567916525 32.40160933819187 31.010888232829625 18.357116861061982 80-81 18.394991619418512 31.580319527880977 31.803372053108976 18.221316799591538 82-83 18.526775311137005 31.854591354498556 31.44488896679487 18.173744367569576 84-85 18.739370747842695 31.6166976272725 31.512870216260335 18.13106140862447 86-87 18.882986898396894 31.539269229070083 31.387804153933185 18.189939718599835 88-89 18.79177226902285 31.631554201007706 31.226030841337572 18.350642688631872 90-91 18.58126344690083 31.725123310254975 31.73006810176517 17.963545141079027 92-93 18.6715855055051 32.025308034245285 31.288200424266215 18.0149060359834 94-95 18.28047606988763 31.710039933841554 31.643019922682463 18.36646407358835 96-97 18.271311326846913 31.889617049190395 31.259489532477108 18.579582091485587 98-99 18.12743050592884 32.191121439654914 31.488036790899994 18.193411263516253 100-101 18.281771108942685 32.15409646894011 31.266915447796755 18.297216974320445 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18123.0 1 40568.5 2 62453.0 3 72985.0 4 98005.0 5 129012.5 6 160083.5 7 186718.0 8 210606.0 9 231037.5 10 248210.5 11 262636.5 12 274090.5 13 281502.5 14 286592.5 15 292627.5 16 297339.0 17 303137.5 18 315035.5 19 331829.5 20 361680.0 21 404083.0 22 461838.5 23 535428.5 24 654878.0 25 812862.5 26 997047.5 27 1234186.0 28 1567006.5 29 1768478.5 30 1915532.0 31 2159798.0 32 2483351.0 33 2662789.0 34 2517016.0 35 2479064.5 36 2452711.5 37 2492089.5 38 2589194.0 39 2376823.0 40 2216125.0 41 2075735.0 42 1750346.5 43 1555902.0 44 1441665.0 45 1342527.0 46 1281423.0 47 1229030.5 48 1185242.5 49 1133352.0 50 1020467.5 51 866676.0 52 747822.0 53 644692.0 54 538867.5 55 426290.0 56 353428.5 57 305861.0 58 254922.0 59 204818.0 60 163297.5 61 119878.0 62 82947.0 63 66418.5 64 52521.5 65 38464.5 66 31068.0 67 23263.5 68 13793.0 69 8480.0 70 6814.5 71 7762.5 72 6813.0 73 8414.0 74 8652.5 75 4598.5 76 3277.5 77 3450.5 78 3165.0 79 2027.0 80 1517.0 81 986.0 82 621.0 83 398.0 84 250.5 85 176.5 86 141.0 87 110.0 88 86.5 89 68.5 90 56.0 91 50.0 92 50.5 93 47.0 94 37.5 95 32.5 96 25.5 97 15.5 98 9.5 99 10.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006923082442633773 2 0.011976778130983346 3 0.006857851316221653 4 0.009961479646566771 5 0.0075599442294468485 6 0.065337556144688 7 0.05386374433051389 8 0.06949174893198622 9 0.05992680586966155 10-11 0.051480233303586795 12-13 0.0608014179461609 14-15 0.07576252011049879 16-17 0.08516781857397274 18-19 0.08460906247799523 20-21 0.05599920541621596 22-23 0.060755927818531404 24-25 0.07552133660363292 26-27 0.05797673851165709 28-29 0.07880091731443178 30-31 0.065966693192847 32-33 0.06257639122800651 34-35 0.06873472454388765 36-37 0.07940001371226942 38-39 0.06809099632271541 40-41 0.06784294638149037 42-43 0.08109173147751007 44-45 0.10901065358189779 46-47 0.09157076861389933 48-49 0.08244699395915138 50-51 0.10250127380940406 52-53 0.11644356877569999 54-55 0.11990339338842709 56-57 0.11028781037270352 58-59 0.11447891024468229 60-61 0.10977282779576573 62-63 0.12902974295605973 64-65 0.10665975811817675 66-67 0.08294738536307593 68-69 0.11668990210833524 70-71 0.12036001127331193 72-73 0.10599028076815763 74-75 0.10364882665168045 76-77 0.10822273023918297 78-79 0.09624166058672515 80-81 0.11536725518989999 82-83 0.11088948168342587 84-85 0.10256135511004681 86-87 0.11851036551781034 88-89 0.11903049792051752 90-91 0.10617824940873993 92-93 0.09375944456588498 94-95 0.11936866981270668 96-97 0.13185013086908906 98-99 0.1201797673713837 100-101 0.09886635512051811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.8254398E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.64998944357388 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80849359397209 14.511275841479653 2 8.411893686262797 3.137634568985338 3 3.3281080261490024 1.8620753866425719 4 1.8805359663145818 1.4028790368011181 5 1.2603389587921252 1.1752654138399015 6 0.8714826542565594 0.9751885381285549 7 0.6498361733539216 0.8483606441172161 8 0.5265865983623514 0.7856667600468267 9 0.4434465948395221 0.7443246881291283 >10 3.6533337002773476 14.04695320837309 >50 0.5599334349969479 7.341038096135784 >100 0.501349110485692 19.02776751029246 >500 0.061426673251800894 7.920118080409141 >1k 0.03792807464946353 13.440198616472864 >5k 0.0032229504544942743 4.169418233523725 >10k+ 0.002083803581378626 8.611835376622764 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 333639 0.5727275732898313 Illumina Single End PCR Primer 1 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130334 0.22373246394203575 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 99901 0.17149091472887593 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 80039 0.13739563491841422 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 74695 0.12822207861456228 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 65982 0.11326526797169889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.6671118633824008 0.0 0.0 1.716608589792654E-6 0.0 2 0.6709296008860997 1.716608589792654E-6 0.0 1.716608589792654E-6 0.0 3 0.6711218610481564 1.716608589792654E-6 0.0 1.716608589792654E-6 0.0 4 0.674676957437617 1.716608589792654E-6 0.0 3.433217179585308E-6 0.0 5 0.6750855102819876 1.716608589792654E-6 0.0 5.149825769377962E-6 0.0 6 0.6759197820566268 1.716608589792654E-6 0.0 5.149825769377962E-6 0.0 7 0.6764673801967708 1.716608589792654E-6 0.0 6.866434359170616E-6 0.0 8 0.6788036844874785 1.716608589792654E-6 0.0 6.866434359170616E-6 0.0 9 0.6793821815822386 1.716608589792654E-6 0.0 6.866434359170616E-6 0.0 10-11 0.6816566879637139 1.716608589792654E-6 0.0 6.866434359170616E-6 0.0 12-13 0.6834136368553667 1.716608589792654E-6 0.0 6.866434359170616E-6 0.0 14-15 0.6850186658868228 1.716608589792654E-6 0.0 6.866434359170616E-6 0.0 16-17 0.6875738377727292 1.716608589792654E-6 0.0 8.58304294896327E-6 0.0 18-19 0.6899324579751043 1.716608589792654E-6 0.0 1.0299651538755924E-5 0.0 20-21 0.6938566252113703 2.574912884688981E-6 0.0 1.2016260128548577E-5 0.0 22-23 0.6973851141676891 3.433217179585308E-6 0.0 1.2016260128548577E-5 0.0 24-25 0.6993025659624875 3.433217179585308E-6 0.0 1.2874564423444905E-5 0.0 26-27 0.7011668028910023 3.433217179585308E-6 0.0 1.6307781603030214E-5 0.0 28-29 0.7028319132231011 3.433217179585308E-6 8.58304294896327E-7 1.716608589792654E-5 0.0 30-31 0.70433222913058 5.149825769377962E-6 1.716608589792654E-6 1.716608589792654E-5 0.0 32-33 0.705880610078573 6.0081300642742895E-6 5.149825769377962E-6 1.716608589792654E-5 0.0 34-35 0.7074238412007965 6.866434359170616E-6 7.724738654066942E-6 2.059930307751185E-5 0.0 36-37 0.709025437015073 6.866434359170616E-6 9.441347243859597E-6 2.3174215962200826E-5 0.0 38-39 0.7109394555926919 6.866434359170616E-6 1.0299651538755924E-5 2.746573743668246E-5 0.0 40-41 0.7129023975151199 6.866434359170616E-6 1.2016260128548577E-5 3.9481997565231046E-5 0.0 42-43 0.7149992349075516 6.866434359170616E-6 1.2016260128548577E-5 6.0939604937639214E-5 0.0 44-45 0.7171261129503046 6.866434359170616E-6 1.2016260128548577E-5 8.068060372025473E-5 0.0 46-47 0.7193705786814585 6.866434359170616E-6 1.2016260128548577E-5 9.698838532328494E-5 0.0 48-49 0.7217249073623591 8.58304294896327E-6 1.2016260128548577E-5 1.0042160250287025E-4 0.0 50-51 0.7243204195501256 8.58304294896327E-6 1.2016260128548577E-5 1.0385481968245556E-4 0.0 52-53 0.7270790095539225 8.58304294896327E-6 1.2016260128548577E-5 1.081463411569372E-4 0.0 54-55 0.7301783463627931 8.58304294896327E-6 1.2016260128548577E-5 1.081463411569372E-4 0.0 56-57 0.7335952557607753 1.0299651538755924E-5 1.2016260128548577E-5 1.0986294974672986E-4 0.0 58-59 0.7373305960521641 1.115795583365225E-5 1.2016260128548577E-5 1.1243786263141883E-4 0.0 60-61 0.7414581814063206 1.2016260128548577E-5 1.2016260128548577E-5 1.1587107981100414E-4 1.716608589792654E-6 62-63 0.7460114856907456 1.2016260128548577E-5 1.2016260128548577E-5 1.1672938410590047E-4 1.716608589792654E-6 64-65 0.7509553184293485 1.2874564423444905E-5 1.2016260128548577E-5 1.1672938410590047E-4 1.716608589792654E-6 66-67 0.7566510257302805 1.3732868718341232E-5 1.2016260128548577E-5 1.1844599269569313E-4 1.716608589792654E-6 68-69 0.7630891662462979 1.3732868718341232E-5 1.2016260128548577E-5 1.2016260128548578E-4 1.716608589792654E-6 70-71 0.7698800698275176 1.3732868718341232E-5 1.2016260128548577E-5 1.2016260128548578E-4 1.716608589792654E-6 72-73 0.7780245192817888 1.3732868718341232E-5 1.2016260128548577E-5 1.2016260128548578E-4 1.716608589792654E-6 74-75 0.7869131185597351 1.3732868718341232E-5 1.2016260128548577E-5 1.2016260128548578E-4 1.716608589792654E-6 76-77 0.7970239431536139 1.3732868718341232E-5 1.2016260128548577E-5 1.210209055803821E-4 1.716608589792654E-6 78-79 0.808671132435357 1.3732868718341232E-5 1.2016260128548577E-5 1.2187920987527843E-4 1.716608589792654E-6 80-81 0.8218812938381064 1.3732868718341232E-5 1.2016260128548577E-5 1.2359581846507108E-4 1.716608589792654E-6 82-83 0.8367110754453251 1.5449477308133885E-5 1.2016260128548577E-5 1.2359581846507108E-4 3.433217179585308E-6 84-85 0.8540616967666544 1.5449477308133885E-5 1.2016260128548577E-5 1.2445412275996742E-4 4.291521474481635E-6 86-87 0.8738567687198484 1.5449477308133885E-5 1.2016260128548577E-5 1.2531242705486373E-4 5.149825769377962E-6 88-89 0.8967305781788355 1.5449477308133885E-5 1.2016260128548577E-5 1.2960394852934537E-4 5.149825769377962E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 45305 0.0 63.46147 1 AGATCGG 65960 0.0 58.135292 1 GATCGGA 68755 0.0 55.56896 2 AGAGCGT 68350 0.0 55.483673 9 ATCGGAA 71495 0.0 53.431614 3 GTTTTTT 1046530 0.0 49.2548 1 TCGGAAG 78895 0.0 47.789192 4 GCGTGTT 66895 0.0 45.339188 2 CGGAAGA 83645 0.0 45.06976 5 AAGAGCG 90310 0.0 42.343765 8 CGCCGTA 49360 0.0 42.016644 46-47 GAAGAGC 93880 0.0 40.217052 7 CCGTATC 52350 0.0 39.774567 48-49 GTCGCCG 52035 0.0 39.380695 44-45 GCCGTAT 52775 0.0 36.758263 48-49 GGTCGCC 52470 0.0 36.65972 44-45 TGGTCGC 55035 0.0 36.50361 42-43 TCTCGGT 56685 0.0 36.453873 36-37 TCGCCGT 52440 0.0 36.2199 46-47 TCGGTGG 57715 0.0 36.121628 38-39 >>END_MODULE