FastQCFastQC Report
Fri 27 May 2016
SRR1233096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58254398
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3637950.6244936219236186TruSeq Adapter, Index 1 (100% over 49bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1271230.21822043376021155No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1030430.17688449891800445No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT816000.14007526092708056No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA753380.12932585793779897No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC639670.10980630166326669TruSeq Adapter, Index 1 (100% over 50bp)
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT629280.10802274533847213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT450800.064.955211
AGATCGG674350.058.9084851
GATCGGA745350.054.0101282
ATCGGAA767300.052.4340063
AGAGCAC818700.049.047919
TCGGAAG857800.046.8565184
GTTTTTT8939700.045.9191861
GCGTGTT679250.045.769042
CGGAAGA906300.044.338545
GAAGAGC1024700.039.2710157
TATGCCG642050.036.54548646-47
AAGAGCA1114700.036.202568
CCGTCTT652350.035.8119450-51
CACACGT690250.034.40635712-13
TGCCGTC682200.034.2623748-49
CGTCTGA710800.033.4483816-17
CACGATC685750.032.04883636-37
GCCGTCT622750.031.84772550-51
CACGTCT747600.031.78285214-15
CATCACG628900.031.66089234-35
Produced by FastQC (version 0.11.5)