FastQCFastQC Report
Fri 27 May 2016
SRR1233095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36829671
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT965640.26219077547556696No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC715990.19440575507720392Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT235850.060.436681
GTTTTTT6326350.047.438271
GCGTGTT327450.043.3154872
CGCCGTA149850.038.07892646-47
CCGTATC156400.036.90830248-49
GTCGCCG163350.034.6312644-45
AGAGCGT265050.034.371669
AGATCGG273700.033.923581
TGGTCGC164600.032.61722642-43
AAGAGCG287350.032.4968728
GATCGGA295200.031.6560362
CGCGCGT10500.031.337931
ATCGGAA300400.031.2509383
TCGGAAG311450.030.067924
TCGGTGG198150.029.40932838-39
CGTGTTT503500.029.001313
TCTCGGT196250.028.9274836-37
CGGAAGA325850.028.7353535
TCGCCGT153750.028.48172646-47
GGTCGCC162100.028.14474544-45
Produced by FastQC (version 0.11.5)