##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36829671 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97011417777802 33.0 31.0 34.0 27.0 34.0 2 31.35777900378203 33.0 31.0 34.0 28.0 34.0 3 31.271408506472948 34.0 31.0 34.0 27.0 34.0 4 34.56105518835615 37.0 35.0 37.0 30.0 37.0 5 34.51058202501999 37.0 35.0 37.0 30.0 37.0 6 34.546718839818034 37.0 35.0 37.0 30.0 37.0 7 34.64377061635984 37.0 35.0 37.0 32.0 37.0 8 34.63829587291182 37.0 35.0 37.0 32.0 37.0 9 36.19149693191666 39.0 37.0 39.0 32.0 39.0 10-11 36.04550264649391 39.0 37.0 39.0 30.0 39.0 12-13 35.96079711111186 39.0 37.0 39.0 30.0 39.0 14-15 37.14599182816485 40.0 37.0 41.0 31.0 41.0 16-17 36.90514472692412 40.0 37.0 41.0 30.0 41.0 18-19 36.4078552860274 40.0 36.0 41.0 28.0 41.0 20-21 36.055298552626226 40.0 35.0 41.0 27.0 41.0 22-23 35.5833902100293 39.5 35.0 41.0 26.0 41.0 24-25 35.04751402748072 39.0 35.0 41.0 21.5 41.0 26-27 34.481791040164325 39.0 34.5 41.0 16.5 41.0 28-29 34.19120222388085 39.0 34.0 41.0 10.0 41.0 30-31 33.90870545653259 40.0 34.0 41.0 8.5 41.0 32-33 33.697771261654765 39.5 34.0 41.0 4.5 41.0 34-35 33.50614576491873 39.0 34.0 41.0 2.0 41.0 36-37 33.34123026784573 39.0 34.0 41.0 2.0 41.0 38-39 33.18435485073978 39.0 33.5 41.0 2.0 41.0 40-41 33.042384481251545 39.0 33.0 41.0 2.0 41.0 42-43 32.8758419128968 39.0 33.0 41.0 2.0 41.0 44-45 32.57091145343112 39.0 33.0 41.0 2.0 41.0 46-47 32.50363391788105 39.0 32.5 41.0 2.0 41.0 48-49 32.37563084666165 39.0 32.0 41.0 2.0 41.0 50-51 31.843893433096376 38.5 31.0 40.5 2.0 40.5 52-53 31.89362005161545 38.5 31.5 40.0 2.0 41.0 54-55 32.19156767650735 39.0 32.0 41.0 2.0 41.0 56-57 32.04647265244373 39.0 32.0 41.0 2.0 41.0 58-59 31.929909813747727 39.0 31.0 41.0 2.0 41.0 60-61 31.839785522384926 38.5 31.0 41.0 2.0 41.0 62-63 31.652116387355186 38.0 31.0 41.0 2.0 41.0 64-65 31.383633809816004 38.0 31.0 41.0 2.0 41.0 66-67 31.086028341116595 37.5 30.5 40.0 2.0 41.0 68-69 30.780580920747298 37.0 30.0 40.0 2.0 41.0 70-71 30.460733779022895 36.5 29.0 40.0 2.0 41.0 72-73 30.13264200758133 36.0 29.0 39.0 2.0 41.0 74-75 29.750569181027984 36.0 29.0 39.0 2.0 41.0 76-77 29.351582016575712 35.0 28.0 39.0 2.0 40.5 78-79 28.963872837745413 35.0 28.0 37.5 2.0 39.5 80-81 28.584832878360494 35.0 27.5 37.0 2.0 39.0 82-83 28.197446102627417 35.0 26.0 36.5 2.0 39.0 84-85 27.81402716032951 35.0 26.0 36.0 2.0 38.0 86-87 27.463218474039586 34.5 26.0 36.0 2.0 37.0 88-89 27.124635311024093 34.0 25.5 35.5 2.0 37.0 90-91 26.817510031517795 34.0 25.0 35.0 2.0 36.5 92-93 26.51049387598385 34.0 24.0 35.0 2.0 36.0 94-95 26.17772878014577 34.0 21.5 35.0 2.0 36.0 96-97 25.819036816810012 34.0 19.0 35.0 2.0 36.0 98-99 25.469694163165347 34.0 6.0 35.0 2.0 35.0 100-101 23.952613627745954 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5702118998207126 1101 2 0.5737814468806484 1101 3 0.6748937146123275 1101 4 0.720289037881436 1101 5 0.7280745020455015 1101 6 0.725426045999086 1101 7 0.6729230270358357 1101 8 0.6377711365659238 1101 9 0.7414990935362056 1101 10-11 0.7385398397578982 1101 12-13 0.7661292750367252 1101 14-15 0.7279633594813077 1101 16-17 0.8561083761399217 1101 18-19 0.8909887048579606 1101 20-21 0.8555464440982306 1101 22-23 0.7834331629925728 1101 24-25 0.8814597559663895 1101 26-27 0.8998047081467035 1101 28-29 0.8843221824804388 1101 30-31 0.8765609547718967 1101 32-33 0.8403691358605414 1101 34-35 0.7924419140464494 1101 36-37 0.7874967387068637 1101 38-39 0.7329328492317444 1101 40-41 0.7000671942728971 1101 42-43 0.7692587555707107 1101 44-45 0.7025448321105472 1101 46-47 0.7244520843384876 1101 48-49 0.6974181504441646 1101 50-51 0.6865927084097514 1101 52-53 0.5998842671290809 1101 54-55 0.6300437527898666 1101 56-57 0.656679394228064 1101 58-59 0.7197113800816624 1101 60-61 0.7438296630874852 1101 62-63 0.7253480020321206 1101 64-65 0.762386723196272 1101 66-67 0.814023797020976 1101 68-69 0.7533124965135585 1101 70-71 0.6774010045740297 1101 72-73 0.7070215161558515 1101 74-75 0.6687043092427203 1101 76-77 0.6799552588701872 1101 78-79 0.714823532307097 1101 80-81 0.6888007731692234 1101 82-83 0.675897470817457 1101 84-85 0.5626458088039428 1101 86-87 0.5922074911633466 1101 88-89 0.6622644444625152 1101 90-91 0.6580664410443617 1101 92-93 0.6851796866285333 1101 94-95 0.7606080396572423 1101 96-97 0.7427963023721347 1101 98-99 0.650457318917244 1101 100-101 0.7157525659307282 1102 1 0.4688137533518244 1102 2 0.5033980299792518 1102 3 0.6071407454704421 1102 4 0.5773056560148078 1102 5 0.6386795904928206 1102 6 0.600044658539538 1102 7 0.6003633424997545 1102 8 0.6384044106694873 1102 9 0.7839610478129728 1102 10-11 0.8134722868417299 1102 12-13 0.788021700108068 1102 14-15 0.786104730996442 1102 16-17 0.8264547477103221 1102 18-19 0.9035488028788876 1102 20-21 0.8300270054660075 1102 22-23 0.8501591722401685 1102 24-25 0.8310253075749614 1102 26-27 0.8732943910629629 1102 28-29 0.7810019683071161 1102 30-31 0.8294311495969495 1102 32-33 0.8260271501407317 1102 34-35 0.8374258597808648 1102 36-37 0.7799745787166117 1102 38-39 0.7349010266374023 1102 40-41 0.7890833687174705 1102 42-43 0.7598996793713724 1102 44-45 0.6871179449422797 1102 46-47 0.7291492057788957 1102 48-49 0.7445451708509836 1102 50-51 0.6536513086739326 1102 52-53 0.6494054430344924 1102 54-55 0.7385324051781552 1102 56-57 0.7938019227065496 1102 58-59 0.8382686200079945 1102 60-61 0.8280441940366892 1102 62-63 0.7882308411236991 1102 64-65 0.8389286243359138 1102 66-67 0.8641109804600013 1102 68-69 0.8871447609499015 1102 70-71 0.8292440498203248 1102 72-73 0.7569363914983605 1102 74-75 0.8278024901504821 1102 76-77 0.784095244278582 1102 78-79 0.8215254966603389 1102 80-81 0.7859565027978057 1102 82-83 0.8156813606199691 1102 84-85 0.8012881651999493 1102 86-87 0.7838096430494765 1102 88-89 0.7383149331911412 1102 90-91 0.7385785804636278 1102 92-93 0.7674537850507512 1102 94-95 0.7466587812984891 1102 96-97 0.80222295673342 1102 98-99 0.8084230103191707 1102 100-101 0.9074449635000903 1103 1 0.33869967849382476 1103 2 0.4047024632369194 1103 3 0.37460740233887435 1103 4 0.5319243101919042 1103 5 0.36786437305516984 1103 6 0.4913917360410949 1103 7 0.5135006335474941 1103 8 0.533422501863619 1103 9 0.5776739285861936 1103 10-11 0.61171488490141 1103 12-13 0.6489114098174937 1103 14-15 0.7162945209616822 1103 16-17 0.7111221075694729 1103 18-19 0.6124056571897754 1103 20-21 0.6969405875415902 1103 22-23 0.6680947927333065 1103 24-25 0.7084214976683612 1103 26-27 0.7338108505014702 1103 28-29 0.6814008540865188 1103 30-31 0.5345525935812176 1103 32-33 0.629709304768646 1103 34-35 0.6983148127787118 1103 36-37 0.6779457670907689 1103 38-39 0.5318396226649327 1103 40-41 0.5990290352595764 1103 42-43 0.6342021810172085 1103 44-45 0.5644801569887221 1103 46-47 0.5294055756586076 1103 48-49 0.5703842234414722 1103 50-51 0.572708243009135 1103 52-53 0.5195063203900041 1103 54-55 0.5842505920878125 1103 56-57 0.5957479042216889 1103 58-59 0.6623330725490533 1103 60-61 0.6738152889667468 1103 62-63 0.6990623980486816 1103 64-65 0.6490130776125369 1103 66-67 0.6424043171841944 1103 68-69 0.630544722556472 1103 70-71 0.6535130908027966 1103 72-73 0.5729553686063689 1103 74-75 0.5707231839746711 1103 76-77 0.5412071282150563 1103 78-79 0.5426262597998281 1103 80-81 0.5160053103030329 1103 82-83 0.5671477445152533 1103 84-85 0.5263028368758071 1103 86-87 0.52914007684738 1103 88-89 0.5048591173061077 1103 90-91 0.5120859779698499 1103 92-93 0.5766389590666527 1103 94-95 0.6109533332518708 1103 96-97 0.6516560618372012 1103 98-99 0.6273676275951701 1103 100-101 0.7618898633832778 1104 1 0.3055252806850497 1104 2 0.25750034153645274 1104 3 0.32718231925203867 1104 4 0.5052341821089215 1104 5 0.5012193578097452 1104 6 0.4685202350245703 1104 7 0.5108362643868531 1104 8 0.5349995246888639 1104 9 0.6168530626440614 1104 10-11 0.5404536176237897 1104 12-13 0.5693251841193074 1104 14-15 0.6409914690841063 1104 16-17 0.6784115119054022 1104 18-19 0.6309281759866252 1104 20-21 0.658852574505886 1104 22-23 0.7149889459683649 1104 24-25 0.6650170102953936 1104 26-27 0.693457230397776 1104 28-29 0.6432080801663247 1104 30-31 0.6054565191238765 1104 32-33 0.5703970287959308 1104 34-35 0.5488184631890078 1104 36-37 0.4348861263399968 1104 38-39 0.5245186622140352 1104 40-41 0.4914450544339175 1104 42-43 0.5207581014110261 1104 44-45 0.4735257499244696 1104 46-47 0.49214812447937817 1104 48-49 0.533877160754308 1104 50-51 0.4855941428898909 1104 52-53 0.3609977016356858 1104 54-55 0.5048595460979541 1104 56-57 0.502212073240635 1104 58-59 0.5241127905572966 1104 60-61 0.5777267744107739 1104 62-63 0.5656609164324777 1104 64-65 0.44509680884411296 1104 66-67 0.5460616725903265 1104 68-69 0.6026782739731935 1104 70-71 0.5155820758356633 1104 72-73 0.5870740726940937 1104 74-75 0.5543798738132395 1104 76-77 0.6218834442260359 1104 78-79 0.5488035975998677 1104 80-81 0.5233930207424464 1104 82-83 0.5581129305091146 1104 84-85 0.5461909677197099 1104 86-87 0.4905794696118839 1104 88-89 0.482831399544537 1104 90-91 0.45893682017226567 1104 92-93 0.47852740437894425 1104 94-95 0.4741847344114376 1104 96-97 0.5176280251096017 1104 98-99 0.5422220416241039 1104 100-101 0.3968278748972729 1105 1 0.2178452621966258 1105 2 0.26350053561746023 1105 3 0.35247089814735943 1105 4 0.4105255621882833 1105 5 0.40536987446648 1105 6 0.45692065563653017 1105 7 0.3969916835243765 1105 8 0.39469808189635813 1105 9 0.44982681666190416 1105 10-11 0.4374815127896241 1105 12-13 0.3870867248849734 1105 14-15 0.4738567727862204 1105 16-17 0.429823442415433 1105 18-19 0.4405845182911534 1105 20-21 0.41281303835035743 1105 22-23 0.4342428240643841 1105 24-25 0.33909062349512453 1105 26-27 0.3600166080986398 1105 28-29 0.39668847039944666 1105 30-31 0.33198018505396476 1105 32-33 0.32418265264403345 1105 34-35 0.20820447368289052 1105 36-37 0.23523849259722596 1105 38-39 0.20236674068585359 1105 40-41 0.35221296328928986 1105 42-43 0.4024538402526545 1105 44-45 0.24604487667661346 1105 46-47 0.3278909475752343 1105 48-49 0.3023250585267405 1105 50-51 0.32171579819909013 1105 52-53 0.27793919044345117 1105 54-55 0.3858378583828781 1105 56-57 0.40173910636961097 1105 58-59 0.31547854985451806 1105 60-61 0.4017728982773683 1105 62-63 0.4566511268175546 1105 64-65 0.43772868145087074 1105 66-67 0.482474479862784 1105 68-69 0.42886905427268474 1105 70-71 0.4172495865412742 1105 72-73 0.47713221060616107 1105 74-75 0.47393253231873445 1105 76-77 0.4981509128890451 1105 78-79 0.42025363756406264 1105 80-81 0.4612685523244977 1105 82-83 0.39690005820854424 1105 84-85 0.4550298890348188 1105 86-87 0.49085388090623994 1105 88-89 0.4919435985856424 1105 90-91 0.4338417649160391 1105 92-93 0.4076012364787758 1105 94-95 0.38883554242609364 1105 96-97 0.31098712449038146 1105 98-99 0.23561002275585352 1105 100-101 0.29574193404351234 1106 1 0.0048412348314599285 1106 2 0.04714436454117532 1106 3 0.08667559256664603 1106 4 0.09749692326015236 1106 5 0.16523860017119318 1106 6 0.13663288911568117 1106 7 0.14760315893326492 1106 8 0.20505266136021305 1106 9 0.21643438834257722 1106 10-11 0.06355730644874313 1106 12-13 0.11236624197743339 1106 14-15 0.09584935804417682 1106 16-17 0.13812481663876497 1106 18-19 0.06836560964556071 1106 20-21 0.13345350982426396 1106 22-23 0.1602257428821119 1106 24-25 -0.0013441357577335111 1106 26-27 0.03606597923204191 1106 28-29 0.18012021320192417 1106 30-31 0.2032319527187454 1106 32-33 0.1818044501806213 1106 34-35 0.08429135538018073 1106 36-37 0.07406486492312325 1106 38-39 0.007349163312312612 1106 40-41 0.05426461035052199 1106 42-43 0.1138036994898215 1106 44-45 0.12645628421431354 1106 46-47 0.030555409093437902 1106 48-49 0.03599905164123385 1106 50-51 0.050450608748985104 1106 52-53 0.043660316986244396 1106 54-55 0.15780474111760157 1106 56-57 0.1891473132928141 1106 58-59 0.04209539015839425 1106 60-61 0.13069992288012244 1106 62-63 0.2402998337580584 1106 64-65 0.217475525918708 1106 66-67 0.2546623164883961 1106 68-69 0.2568505708386333 1106 70-71 0.15255998015739536 1106 72-73 0.18596737744928404 1106 74-75 0.21820556921671397 1106 76-77 0.18639353851018114 1106 78-79 0.16191735809015384 1106 80-81 0.1682684312858207 1106 82-83 0.24069193779833142 1106 84-85 0.2604785923095747 1106 86-87 0.16823569958210172 1106 88-89 0.19817784334663457 1106 90-91 0.23595967881404434 1106 92-93 0.1717386299186039 1106 94-95 0.10505694547332212 1106 96-97 0.09808753504988488 1106 98-99 -0.07437425473785808 1106 100-101 -0.08686930489151834 1107 1 0.05147609980712886 1107 2 0.12371022760391881 1107 3 0.10493293306782903 1107 4 0.16716293099293722 1107 5 0.03118973793938551 1107 6 0.20249479398595582 1107 7 0.16208678919929298 1107 8 0.21472036654463267 1107 9 0.12379831257526774 1107 10-11 0.1971453545666364 1107 12-13 0.19581774719734568 1107 14-15 0.13183639384880763 1107 16-17 0.11721571865046343 1107 18-19 0.1650457721446017 1107 20-21 0.17879988653218248 1107 22-23 0.07019811240824225 1107 24-25 0.045806107276952446 1107 26-27 0.017849202479538917 1107 28-29 0.04490922518004936 1107 30-31 0.002986276531181886 1107 32-33 0.030377763348639064 1107 34-35 6.104918619200816E-4 1107 36-37 0.005758369326635204 1107 38-39 -0.04681534760607775 1107 40-41 0.014747045075225174 1107 42-43 0.07681852744737938 1107 44-45 0.11732568892734463 1107 46-47 0.04136784724799014 1107 48-49 0.04666439436881831 1107 50-51 -0.039346296328258035 1107 52-53 0.014865191602513761 1107 54-55 0.13023734574390033 1107 56-57 0.13651577926151504 1107 58-59 0.15981729892589414 1107 60-61 -0.016980951370950237 1107 62-63 0.04338699997831341 1107 64-65 0.06275902840371828 1107 66-67 0.09331860938823056 1107 68-69 0.1559690550595576 1107 70-71 0.18057237390006975 1107 72-73 0.18301651865312962 1107 74-75 0.15524558882277972 1107 76-77 0.22678419439949238 1107 78-79 0.186892834836474 1107 80-81 0.17628997389326173 1107 82-83 0.13213907105140166 1107 84-85 0.1252268523099076 1107 86-87 0.146352540684358 1107 88-89 0.09397544633242916 1107 90-91 0.12614385321639432 1107 92-93 0.06128472637782423 1107 94-95 0.06317854317774874 1107 96-97 0.18117078913426 1107 98-99 0.06553115234902052 1107 100-101 -0.00958932607348828 1108 1 0.019470018965932923 1108 2 0.07587529982000163 1108 3 0.11481351057207334 1108 4 0.22211981401626701 1108 5 0.09356445410422509 1108 6 0.06220405370886084 1108 7 0.15424765731873435 1108 8 0.10161848621159209 1108 9 0.10644387489956131 1108 10-11 0.1023415956388476 1108 12-13 0.1040742099382399 1108 14-15 0.06528415364059015 1108 16-17 0.0447186503357031 1108 18-19 0.03844587661700416 1108 20-21 0.06831654556022215 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0.42442897759739395 2311 48-49 0.4376723465781538 2311 50-51 0.16855730164299487 2311 52-53 0.1995513424776867 2311 54-55 0.26230196686629625 2311 56-57 0.4747525330903457 2311 58-59 0.4409461930414409 2311 60-61 0.2816773124702614 2311 62-63 0.35988274301918466 2311 64-65 0.4081122710196645 2311 66-67 0.4081921970595914 2311 68-69 0.33052600745408967 2311 70-71 0.3158759211935731 2311 72-73 0.44933671489232907 2311 74-75 0.4076437035984384 2311 76-77 0.3941098633746982 2311 78-79 0.40104064518079596 2311 80-81 0.40410143099876095 2311 82-83 0.3351670603934487 2311 84-85 0.32874966127690897 2311 86-87 0.36069935223786587 2311 88-89 0.37628390365779296 2311 90-91 0.3620696793586866 2311 92-93 0.3450797344943233 2311 94-95 0.2775213314922311 2311 96-97 0.3064324468546644 2311 98-99 0.32891561714807693 2311 100-101 0.31117194119142155 2312 1 0.15885530679348747 2312 2 0.15320805256184755 2312 3 0.1108125264223574 2312 4 0.09706703639786696 2312 5 0.10390966417291025 2312 6 0.09358828120895879 2312 7 0.09054332318496705 2312 8 0.11166438889330976 2312 9 0.09684261164832719 2312 10-11 0.1642530373626272 2312 12-13 0.08813676475056553 2312 14-15 0.13799167691375658 2312 16-17 0.14215121868908653 2312 18-19 0.17672497735213 2312 20-21 0.19764072773713082 2312 22-23 0.16745826816427467 2312 24-25 0.19887942378038304 2312 26-27 0.18148336785240105 2312 28-29 0.22740683190382782 2312 30-31 0.21836838724768626 2312 32-33 0.0502116093141467 2312 34-35 0.18972661044096384 2312 36-37 0.2595757182269338 2312 38-39 0.22648319946185325 2312 40-41 0.2465922031922787 2312 42-43 0.2403782085495365 2312 44-45 0.35525036931398546 2312 46-47 0.2377545591781285 2312 48-49 0.27037034384202485 2312 50-51 0.19624000782632933 2312 52-53 0.25734352031374996 2312 54-55 0.3298174507526497 2312 56-57 0.47804750544954544 2312 58-59 0.2767744458848824 2312 60-61 0.2196095507161111 2312 62-63 0.22806982512805973 2312 64-65 0.3260394923113452 2312 66-67 0.2687282318145243 2312 68-69 0.2237706254711327 2312 70-71 0.18624172238532566 2312 72-73 0.22833544373817105 2312 74-75 0.25009666963193666 2312 76-77 0.2520577175394365 2312 78-79 0.2128389506453452 2312 80-81 0.29155892830743113 2312 82-83 0.2986181709696609 2312 84-85 0.23704806264826317 2312 86-87 0.20894825928252558 2312 88-89 0.20617214105466886 2312 90-91 0.24500871660320556 2312 92-93 0.2510038054064587 2312 94-95 0.2302314107471517 2312 96-97 0.2627333790223503 2312 98-99 0.32825711280894154 2312 100-101 0.33455066321487337 2313 1 -0.06541339471358398 2313 2 -0.08221442697897174 2313 3 -0.11872966645650251 2313 4 -0.12173618863539559 2313 5 -0.136903335911299 2313 6 -0.15147739753287226 2313 7 -0.09415915726475532 2313 8 -0.07988586173753731 2313 9 -0.1580558471650093 2313 10-11 -0.5507870116874614 2313 12-13 -0.22764903752525356 2313 14-15 -0.18194209349492496 2313 16-17 -0.17682551679936154 2313 18-19 -0.11523695342652474 2313 20-21 -0.17323844211631467 2313 22-23 -0.5074708214342039 2313 24-25 -0.5099546787189837 2313 26-27 -0.4365918187480631 2313 28-29 -0.3132201343214618 2313 30-31 -0.17362438967559513 2313 32-33 -0.20628268938555294 2313 34-35 -0.2137442085563066 2313 36-37 -0.21159904613794112 2313 38-39 -0.21265685202131834 2313 40-41 -0.21042777611116037 2313 42-43 -0.20993513204390268 2313 44-45 -0.17681005652401893 2313 46-47 -0.2143398427799923 2313 48-49 -0.19087139874940107 2313 50-51 -0.13777707484988255 2313 52-53 -0.08369570012434124 2313 54-55 -0.21244384819200235 2313 56-57 -0.10173773091913318 2313 58-59 -0.18747279039018494 2313 60-61 -0.20231942918586654 2313 62-63 -0.25934488528528377 2313 64-65 -0.21298570775087455 2313 66-67 -0.22744837455161715 2313 68-69 -0.20323533027240615 2313 70-71 -0.23698311966784757 2313 72-73 -0.2473567387345632 2313 74-75 -0.34663668785589863 2313 76-77 -0.2278295482224273 2313 78-79 -0.2841455415522276 2313 80-81 -0.267034230311193 2313 82-83 -0.17833830980681142 2313 84-85 -0.28557196489025927 2313 86-87 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-0.28550592403849606 2314 44-45 -0.30313353384936903 2314 46-47 -0.30251105622766516 2314 48-49 -0.274669365229137 2314 50-51 -0.30061199784794823 2314 52-53 -0.21473709631299798 2314 54-55 -0.27663339893666716 2314 56-57 -0.20696071592460896 2314 58-59 -0.308585289568299 2314 60-61 -0.36673558742058177 2314 62-63 -0.36750618498143695 2314 64-65 -0.30284326309418574 2314 66-67 -0.40334803470607383 2314 68-69 -0.3940372952713851 2314 70-71 -0.35776890353433544 2314 72-73 -0.3576627446354159 2314 74-75 -0.35361935495795294 2314 76-77 -0.2809142144939898 2314 78-79 -0.25841291054652515 2314 80-81 -0.2604560615596334 2314 82-83 -0.2871072949459865 2314 84-85 -0.2607324230428745 2314 86-87 -0.22966826873328827 2314 88-89 -0.2529999482761838 2314 90-91 -0.29087661848550184 2314 92-93 -0.3275955296802451 2314 94-95 -0.295640280426408 2314 96-97 -0.302089807580586 2314 98-99 -0.36405535997902305 2314 100-101 -0.4775600124482722 2315 1 -1.0595523042916284 2315 2 -0.9504794369196787 2315 3 -1.1515630863414046 2315 4 -1.2680252508320038 2315 5 -1.2380214758407462 2315 6 -1.2656330423084654 2315 7 -1.2183046886344897 2315 8 -1.142710989733395 2315 9 -1.300857453239324 2315 10-11 -1.4044504217432277 2315 12-13 -1.4328133486720773 2315 14-15 -1.6941213016019319 2315 16-17 -1.7696629497105292 2315 18-19 -1.6487754367720342 2315 20-21 -1.7485702054482744 2315 22-23 -1.807131015127112 2315 24-25 -1.8404105094336387 2315 26-27 -1.9162327612681835 2315 28-29 -1.9912607028544542 2315 30-31 -1.9552372434870975 2315 32-33 -1.8870448696828959 2315 34-35 -1.7996434593355701 2315 36-37 -1.7812425861838541 2315 38-39 -1.746452087023762 2315 40-41 -1.7258755206601322 2315 42-43 -1.7902753822853406 2315 44-45 -1.7402366696152143 2315 46-47 -1.7784199543105963 2315 48-49 -1.8010422095041498 2315 50-51 -1.8150046090980538 2315 52-53 -1.7579540560446212 2315 54-55 -1.7400301763251633 2315 56-57 -1.6693303517686928 2315 58-59 -1.6760127466025523 2315 60-61 -1.8365903432607205 2315 62-63 -1.8001444213582687 2315 64-65 -1.7515013909114892 2315 66-67 -1.7218521742671342 2315 68-69 -1.8421900302726222 2315 70-71 -1.9071920791298247 2315 72-73 -1.8751343498120114 2315 74-75 -1.865128108099384 2315 76-77 -1.870610218882348 2315 78-79 -1.878319148635537 2315 80-81 -1.8332594885568376 2315 82-83 -1.833109615930539 2315 84-85 -1.9492745986809439 2315 86-87 -1.9316569296377786 2315 88-89 -1.9165861927324812 2315 90-91 -1.8942780091484615 2315 92-93 -1.9109928883690337 2315 94-95 -1.980249249408601 2315 96-97 -2.3582560815793983 2315 98-99 -2.3495331025386967 2315 100-101 -2.3240566004797607 2316 1 -2.7403843406428905 2316 2 -3.0975796127196595 2316 3 -2.9628373936199743 2316 4 -3.1823838200178933 2316 5 -3.154655255796456 2316 6 -3.2491392794270446 2316 7 -3.0469808323480265 2316 8 -3.16024528285579 2316 9 -3.7849685130352597 2316 10-11 -3.9549757817811653 2316 12-13 -3.869027641425241 2316 14-15 -4.437196967803075 2316 16-17 -4.146456893531727 2316 18-19 -4.216663095370386 2316 20-21 -4.340527998084291 2316 22-23 -4.34857598805835 2316 24-25 -4.208893563282032 2316 26-27 -4.172180888701675 2316 28-29 -4.316522894142654 2316 30-31 -4.295128085454557 2316 32-33 -4.2718557609211985 2316 34-35 -4.319365587223551 2316 36-37 -4.2261868294370615 2316 38-39 -4.13151148963798 2316 40-41 -4.239751170290539 2316 42-43 -4.416954127498176 2316 44-45 -4.255992344611734 2316 46-47 -4.319662772398004 2316 48-49 -4.355741006685932 2316 50-51 -4.294169993712355 2316 52-53 -4.198731419231482 2316 54-55 -4.452291825627796 2316 56-57 -4.420521089952771 2316 58-59 -4.441775839926255 2316 60-61 -4.681749602617582 2316 62-63 -4.629085418479704 2316 64-65 -4.631700549557593 2316 66-67 -4.584945670218634 2316 68-69 -4.526954593721634 2316 70-71 -4.566993064859357 2316 72-73 -4.503403391031551 2316 74-75 -4.467398785780183 2316 76-77 -4.483773591857236 2316 78-79 -4.448803801773952 2316 80-81 -4.204332110346808 2316 82-83 -4.257290619995569 2316 84-85 -4.339263418191777 2316 86-87 -4.325827719178061 2316 88-89 -4.355080565986622 2316 90-91 -4.406982564945519 2316 92-93 -4.378577473303917 2316 94-95 -4.517335127471128 2316 96-97 -4.6427475219691985 2316 98-99 -4.723474134523688 2316 100-101 -4.952262126343811 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 430883.0 3 123821.0 4 119920.0 5 131548.0 6 167304.0 7 253467.0 8 428438.0 9 723856.0 10 850612.0 11 654737.0 12 440644.0 13 333912.0 14 277635.0 15 241686.0 16 223637.0 17 215221.0 18 213039.0 19 218854.0 20 231288.0 21 249320.0 22 271516.0 23 299241.0 24 331719.0 25 348606.0 26 370372.0 27 414426.0 28 465183.0 29 533352.0 30 620238.0 31 730924.0 32 882767.0 33 1092605.0 34 1408408.0 35 1955235.0 36 2932655.0 37 5234306.0 38 8557975.0 39 3832436.0 40 17885.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.0563598792549 17.37539300646484 9.158373421184205 46.40987369309606 2 11.68823828525026 16.260928585616057 61.93513010141849 10.115703027715186 3 16.07634052377084 21.758017870871964 41.75032521243335 20.41531639292384 4 17.86821900602062 29.40815427217035 36.71457943815221 16.009047283656816 5 19.27751560452169 30.35615384967479 36.123386065140885 14.242944480662642 6 14.68780494638248 33.63429105962999 37.85202687383878 13.825877120148755 7 12.468810283110312 18.44514173900266 54.46417598214139 14.62187199574563 8 12.79068941994152 20.998989379385595 48.32808340262419 17.8822377980487 9 14.142195499548741 20.80188890544656 46.902453287534435 18.153462307470267 10-11 18.15324753291835 30.54177540551822 35.65724240090379 15.64773466065964 12-13 16.699253784474344 24.63965888782647 41.963298535260975 16.697788792438214 14-15 16.72219857302222 26.35200470959495 40.9946236467732 15.931173070609637 16-17 17.72373857639104 26.573173185748942 39.28226764479703 16.420820593062988 18-19 18.070773006233768 27.30392992159939 38.493658180476025 16.131638891690812 20-21 17.98475351430438 27.649691074766263 37.492778724106586 16.872776686822768 22-23 18.5446196460151 27.924747610191226 36.858874944446775 16.671757799346906 24-25 18.07982877648114 28.71613779761083 36.03468239226002 17.169351033648017 26-27 17.885459921346524 29.147281280511518 35.53117970859524 17.436079089546723 28-29 18.249864239650176 29.652950293971426 34.51789324198572 17.579292224392688 30-31 18.16550763960013 29.799146831882663 34.183554325972814 17.8517912025444 32-33 18.65525157190292 30.0182717943274 33.52044229728413 17.806034336485553 34-35 18.85918325164773 30.48207958174271 33.01575185941073 17.642985307198835 36-37 18.690877148053907 29.976643777517864 33.525120477268075 17.807358597160157 38-39 18.759043000017122 29.891337333120084 33.34001141538617 18.009608251476624 40-41 18.984286180415285 29.579870194667745 33.61781619566476 17.818027429252208 42-43 19.070611615166698 29.883528143719545 33.42531525637673 17.620544984737034 44-45 19.258723672101585 29.626194857336884 33.39871190884286 17.716369561718665 46-47 18.628038572178042 29.81521704214672 33.23339379151567 18.323350594159567 48-49 18.759170892908138 30.06707509442592 32.637523011623344 18.536231001042598 50-51 18.38449685858986 30.241881853295393 32.9110604520472 18.462560836067553 52-53 18.513856455951128 30.62367663283569 32.53723012429692 18.325236786916268 54-55 18.452827328173214 30.725921364415587 32.142074070698165 18.67917723671303 56-57 18.694180737527432 30.63617802806961 32.276043951974714 18.393597282428235 58-59 18.7309446521086 30.61553240450819 32.03722948162269 18.61629346176052 60-61 18.707461589871404 31.187725962909763 31.517033459288136 18.587778987930694 62-63 18.835531984544197 31.195243390243437 31.729740176660133 18.239484448552233 64-65 19.04751191789985 30.821380519285775 31.77700653178049 18.354101031033885 66-67 18.843495879812245 31.659055630024284 31.13035473291546 18.36709375724801 68-69 19.010556075136236 30.888991181925068 31.909857715671187 18.190595027267513 70-71 18.960899622397648 30.98075827823156 31.520973774077977 18.537368325292825 72-73 18.555625850335232 31.785207007174932 31.176445182282087 18.482721960207744 74-75 18.667240601254832 30.793165387156048 31.995211056598315 18.544382954990805 76-77 18.75099779250384 31.109516455008272 31.40893438288195 18.730551369605937 78-79 18.655111914615947 31.451613801936933 31.176919194915914 18.716355088531206 80-81 18.919224422015056 30.860003809636094 31.448638382598702 18.77213338575014 82-83 19.011273777032365 31.08939200698036 31.105738809081323 18.793595406905954 84-85 18.78037882205891 31.243902970930804 31.271371745004284 18.704346462006 86-87 19.00824678511874 31.171075938789578 31.036524101685643 18.78415317440604 88-89 19.00177853015706 31.300160402403883 30.999046983523414 18.699014083915646 90-91 18.970966945523582 31.350180728176653 31.067572134459944 18.61128019183982 92-93 18.974088457625214 31.52344307090563 30.774639953749837 18.727828517719324 94-95 19.011305348300905 31.40891715803395 30.874251165178197 18.70552632848695 96-97 18.80624497637825 31.72961321997691 30.520010926171327 18.944130877473516 98-99 18.708782102876263 31.880464898722888 30.776846380926905 18.63390661747395 100-101 19.022392491334454 32.19359033936993 29.96287919390082 18.821137975394798 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18503.0 1 29288.5 2 35005.5 3 34209.0 4 42708.0 5 52156.5 6 61796.0 7 71705.0 8 82140.5 9 92683.5 10 103507.5 11 113687.5 12 124267.5 13 134164.5 14 143787.5 15 155516.5 16 167477.5 17 182008.0 18 201905.5 19 228446.5 20 264121.5 21 305711.5 22 356232.0 23 420492.0 24 499269.5 25 594774.5 26 697601.5 27 790898.0 28 915857.5 29 1031271.5 30 1138363.5 31 1253621.5 32 1348872.5 33 1422540.0 34 1439319.5 35 1493325.5 36 1563636.5 37 1587499.0 38 1603628.0 39 1502169.5 40 1410286.0 41 1335516.5 42 1195378.0 43 1108948.0 44 1026207.0 45 952034.0 46 896393.5 47 834970.5 48 775353.0 49 717813.5 50 646914.0 51 572185.0 52 509917.0 53 461527.0 54 410314.0 55 348345.5 56 291590.5 57 242411.5 58 198932.0 59 157133.0 60 115597.5 61 83702.0 62 62271.5 63 44690.0 64 28754.0 65 19336.0 66 13534.5 67 9508.0 68 7004.5 69 5185.5 70 4810.5 71 5880.5 72 5675.0 73 7909.0 74 8350.0 75 4821.5 76 3574.5 77 3616.0 78 3357.5 79 2078.5 80 1220.5 81 636.5 82 361.5 83 202.0 84 116.5 85 88.5 86 74.5 87 44.0 88 30.0 89 27.0 90 23.0 91 24.0 92 21.5 93 18.0 94 13.0 95 8.5 96 8.0 97 6.0 98 3.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6782629147026592 2 0.31831671806136963 3 0.3169862690329219 4 0.35427685465884284 5 0.34267751129245766 6 0.1793010858011737 7 0.16274921380644425 8 0.17147587335222192 9 0.16985761290129364 10-11 0.17904178400073137 12-13 0.1949297890822864 14-15 0.18727427676451414 16-17 0.17253072936763406 18-19 0.18800873893225928 20-21 0.21656316180505658 22-23 0.17141342370394783 24-25 0.1856275066915477 26-27 0.17611615373919579 28-29 0.17268006548307205 30-31 0.1919077691462408 32-33 0.1462489306515934 34-35 0.176303502684018 36-37 0.16763386238231667 38-39 0.1700463194471653 40-41 0.16791895860269834 42-43 0.1396428439450355 44-45 0.504792725408815 46-47 0.1319601796062745 48-49 0.10964664875773666 50-51 0.11201837779110217 52-53 0.5599751352652593 54-55 0.3873697378399063 56-57 0.279030458892777 58-59 0.23364042540591798 60-61 0.06626450722299419 62-63 0.07031015835031489 64-65 0.06830905440344553 66-67 0.07770229606449648 68-69 0.09369755162895699 70-71 0.10333244627680763 72-73 0.13691813863881652 74-75 0.10954482867902891 76-77 0.09760880025238346 78-79 0.10489368748365958 80-81 0.09810160943332892 82-83 0.08260866625715989 84-85 0.08559267336382125 86-87 0.09988685481333787 88-89 0.09340159460017983 90-91 0.11319270269886472 92-93 0.1231860040237666 94-95 0.14332330039005778 96-97 0.10618476608167365 98-99 0.1301654310189195 100-101 0.14351607973907776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.6829671E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.0739315669704 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62872229784867 24.898583266125474 2 8.62423123007147 5.532260045820825 3 3.630523382924528 3.493354756086215 4 1.9898631561524605 2.55290938792279 5 1.320321094746207 2.117394421965864 6 0.9615054253634607 1.8503555528627043 7 0.720292795827161 1.61718352890795 8 0.5787365548241281 1.484988532378664 9 0.45732836562075996 1.3201486832299205 >10 3.436722673232713 21.869304267913865 >50 0.38827255087564133 8.655025646952318 >100 0.2416348589446357 14.357479274764383 >500 0.014563514643087697 3.194606780226123 >1k 0.0064553078209965255 3.843535414583457 >5k 5.199408005536358E-4 1.1306069123127185 >10k+ 3.06850303351006E-4 2.08226352794683 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96564 0.26219077547556696 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 71599 0.19440575507720392 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.2554326374514722 0.0 0.0 8.145606296618832E-6 0.0 2 0.25624448287903523 8.145606296618832E-6 0.0 1.6291212593237665E-5 0.0 3 0.2564182558133631 8.145606296618832E-6 0.0 1.6291212593237665E-5 2.715202098872944E-6 4 0.2629103040317683 8.145606296618832E-6 0.0 1.6291212593237665E-5 2.715202098872944E-6 5 0.26327685631511616 8.145606296618832E-6 0.0 1.6291212593237665E-5 2.715202098872944E-6 6 0.2636732758215516 1.357601049436472E-5 0.0 1.6291212593237665E-5 2.715202098872944E-6 7 0.2640669801258882 1.357601049436472E-5 0.0 1.6291212593237665E-5 2.715202098872944E-6 8 0.2663423194847437 1.6291212593237665E-5 0.0 1.6291212593237665E-5 2.715202098872944E-6 9 0.26667085893870734 1.6291212593237665E-5 0.0 1.6291212593237665E-5 2.715202098872944E-6 10-11 0.26863259245514304 1.7648813642674135E-5 0.0 2.3079217840420026E-5 2.715202098872944E-6 12-13 0.2707246556723246 1.9006414692110607E-5 0.0 2.715202098872944E-5 2.715202098872944E-6 14-15 0.272554701886965 1.9006414692110607E-5 0.0 2.9867223087602384E-5 2.715202098872944E-6 16-17 0.2749888805686046 1.9006414692110607E-5 0.0 3.258242518647533E-5 2.715202098872944E-6 18-19 0.2773090207620915 1.9006414692110607E-5 0.0 3.5297627285348276E-5 2.715202098872944E-6 20-21 0.2800432292756566 1.9006414692110607E-5 0.0 3.5297627285348276E-5 2.715202098872944E-6 22-23 0.28297836274453825 1.9006414692110607E-5 0.0 3.5297627285348276E-5 2.715202098872944E-6 24-25 0.28557816875420905 1.9006414692110607E-5 0.0 3.665522833478475E-5 2.715202098872944E-6 26-27 0.28835446290030664 1.9006414692110607E-5 0.0 3.8012829384221215E-5 2.715202098872944E-6 28-29 0.2904587445269332 1.9006414692110607E-5 0.0 4.072803148309416E-5 2.715202098872944E-6 30-31 0.29271371987004713 2.036401574154708E-5 0.0 4.072803148309416E-5 2.715202098872944E-6 32-33 0.29480035268303106 2.1721616790983553E-5 0.0 4.072803148309416E-5 2.715202098872944E-6 34-35 0.29709198325447983 2.3079217840420026E-5 0.0 4.072803148309416E-5 2.715202098872944E-6 36-37 0.299406693043769 2.4436818889856496E-5 0.0 4.2085632532530634E-5 2.715202098872944E-6 38-39 0.30198477743664887 2.4436818889856496E-5 0.0 4.480083463140357E-5 2.715202098872944E-6 40-41 0.30487782527299795 2.4436818889856496E-5 0.0 4.751603673027652E-5 2.715202098872944E-6 42-43 0.3076541194190956 2.579441993929297E-5 0.0 4.887363777971299E-5 2.715202098872944E-6 44-45 0.3107413042055141 2.715202098872944E-5 0.0 4.887363777971299E-5 2.715202098872944E-6 46-47 0.3148236105611696 2.715202098872944E-5 0.0 4.887363777971299E-5 2.715202098872944E-6 48-49 0.31809950189345976 2.715202098872944E-5 0.0 4.887363777971299E-5 2.715202098872944E-6 50-51 0.32145956449081503 2.715202098872944E-5 0.0 5.0231238829149464E-5 2.715202098872944E-6 52-53 0.32490651355533423 2.715202098872944E-5 0.0 5.158883987858594E-5 2.715202098872944E-6 54-55 0.32878246455147536 2.715202098872944E-5 0.0 5.5661643026895356E-5 2.715202098872944E-6 56-57 0.3329733789910857 2.715202098872944E-5 0.0 5.701924407633182E-5 2.715202098872944E-6 58-59 0.33755664013398323 2.715202098872944E-5 0.0 5.701924407633182E-5 2.715202098872944E-6 60-61 0.3422729461797256 2.715202098872944E-5 0.0 5.701924407633182E-5 5.430404197745888E-6 62-63 0.34763139752185135 2.715202098872944E-5 0.0 5.701924407633182E-5 5.430404197745888E-6 64-65 0.35334418273788004 2.715202098872944E-5 0.0 5.973444617520477E-5 5.430404197745888E-6 66-67 0.3592918329354612 2.715202098872944E-5 0.0 6.516485037295066E-5 5.430404197745888E-6 68-69 0.36589791964201906 2.715202098872944E-5 0.0 6.516485037295066E-5 5.430404197745888E-6 70-71 0.3733850894296612 2.850962203816591E-5 0.0 6.516485037295066E-5 5.430404197745888E-6 72-73 0.38190267841382564 2.9867223087602384E-5 0.0 6.652245142238714E-5 5.430404197745888E-6 74-75 0.39137194573364503 2.9867223087602384E-5 0.0 7.059525457069655E-5 5.430404197745888E-6 76-77 0.40211328523678636 2.9867223087602384E-5 0.0 7.331045666956948E-5 5.430404197745888E-6 78-79 0.414323549075418 2.9867223087602384E-5 0.0 7.331045666956948E-5 5.430404197745888E-6 80-81 0.42840051435702475 2.9867223087602384E-5 0.0 7.602565876844243E-5 5.430404197745888E-6 82-83 0.44477725581637695 2.9867223087602384E-5 0.0 7.874086086731538E-5 5.430404197745888E-6 84-85 0.46420452683381286 2.9867223087602384E-5 0.0 8.145606296618832E-5 8.145606296618832E-6 86-87 0.48666060579254156 2.9867223087602384E-5 0.0 8.281366401562479E-5 8.145606296618832E-6 88-89 0.5134515049021209 3.258242518647533E-5 0.0 8.417126506506127E-5 8.145606296618832E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 23585 0.0 60.43668 1 GTTTTTT 632635 0.0 47.43827 1 GCGTGTT 32745 0.0 43.315487 2 CGCCGTA 14985 0.0 38.078926 46-47 CCGTATC 15640 0.0 36.908302 48-49 GTCGCCG 16335 0.0 34.63126 44-45 AGAGCGT 26505 0.0 34.37166 9 AGATCGG 27370 0.0 33.92358 1 TGGTCGC 16460 0.0 32.617226 42-43 AAGAGCG 28735 0.0 32.496872 8 GATCGGA 29520 0.0 31.656036 2 CGCGCGT 1050 0.0 31.33793 1 ATCGGAA 30040 0.0 31.250938 3 TCGGAAG 31145 0.0 30.06792 4 TCGGTGG 19815 0.0 29.409328 38-39 CGTGTTT 50350 0.0 29.00131 3 TCTCGGT 19625 0.0 28.92748 36-37 CGGAAGA 32585 0.0 28.735353 5 TCGCCGT 15375 0.0 28.481726 46-47 GGTCGCC 16210 0.0 28.144745 44-45 >>END_MODULE