FastQCFastQC Report
Fri 27 May 2016
SRR1233095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36829671
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT789310.21431361686614034No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG749750.20357227736299896TruSeq Adapter, Index 8 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT239550.063.7537771
GCGTGTT332750.046.6531682
GTTTTTT5714350.044.2097741
AGATCGG251500.035.8457831
TATGCCG198950.034.61780546-47
TGCCGTC202600.032.8447148-49
GATCGGA290350.032.3803372
ATCGGAA301400.031.2103023
CCGTCTT216250.031.03448150-51
CGTGTTT516600.030.7219963
CTCGTAT213400.030.55338342-43
TCGGAAG315400.030.2784354
CGTATGC232950.029.63169544-45
CGTCTGA246000.029.61085516-17
CGGAAGA335650.029.0064535
CGCGCGT14250.028.3175131
ATCTCGT233450.027.46091740-41
CACGTCT268600.027.2518614-15
CACACGT301350.024.38453912-13
ATGCCGT198000.022.63723248-49
Produced by FastQC (version 0.11.5)