FastQCFastQC Report
Fri 27 May 2016
SRR1233094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56215129
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2848160.5066536447866197Illumina Single End PCR Primer 1 (100% over 50bp)
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT2184030.3885128503396301No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1781800.3169609376863655No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1679130.2986971710053356No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1635860.29099995483422264No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1596820.2840552051388159No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA1477170.2627708992716178No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG1307030.2325050254709902No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1132270.2014173088529246No Hit
CTCACTATAGGAATTTAATACGACTCACTATAGGAATTTAATACGACTCA1099040.19550608876126568No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1095780.1949261736996103No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1018010.18109181960607082No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG1004430.17867609980935917No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT983160.17489242086414142No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT953190.1695611158341378No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT943560.16784805385753007No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT902090.16047103618671762No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT899480.16000674836128187No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA886210.1576461738618442No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA880420.15661620201921087No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT844150.1501642022381555No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT821130.14606921919542334No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT817490.14542170667259344No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC797750.14191019645263112No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT736280.13097541766736853No Hit
CTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACGACT730660.129975686794208No Hit
ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA721610.1283657998899193No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT711250.12652287963263412No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC710890.12645883993257404No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC697900.12414807408873865No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA681890.12130008631662129No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT673390.11978803784297995No Hit
GTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTAT662420.11783660587170405No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA645610.11484630765500868No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT598200.10641263493320453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT668900.050.540329
AAGAGCG680250.050.2514538
GTTTTTT7704450.048.5023881
AGATCGG720000.047.4683571
GATCGGA733400.046.5366862
CGCGTGT413000.046.4532971
ATCGGAA753000.045.47893
TCGGAAG767950.044.3717424
CGGAAGA786000.043.335385
CGCCGTA452350.039.7880746-47
GTCGCCG454700.039.66930444-45
CCGTATC455250.039.5462648-49
GCGTGTT485900.039.474412
TGGTCGC435750.039.45073742-43
GAAGAGC910650.037.5263027
CCGATCT65950.037.1555442
GTGGTCG443050.037.09065642-43
CGTATCA458550.037.01254750-51
GGTCGCC459950.036.7377244-45
CTCGGTG464050.036.2523138-39
Produced by FastQC (version 0.11.5)