FastQCFastQC Report
Fri 27 May 2016
SRR1233094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56215129
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG3275690.5827061252496637TruSeq Adapter, Index 7 (100% over 49bp)
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT2213880.393822808802947No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1808180.3216536245963253No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1808180.3216536245963253No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA1608830.28619164068804326No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1597790.28422775655286675No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1540930.2741130417044849No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG1320700.23493675519271692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1187670.2112723071399516TruSeq Adapter, Index 7 (100% over 50bp)
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1164160.20709015894991545No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1130570.20111489915819636No Hit
CTCACTATAGGAATTTAATACGACTCACTATAGGAATTTAATACGACTCA1064430.18934938315270966No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT998000.1775322796110634No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT980040.17433741013028717No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG980030.17433563124972995No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT933390.16603893233083214No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA919670.16359830820631935No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT910510.16196885361590116No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA910340.16193861264642834No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT881420.1567940900749334No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT879510.1564543238885034No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT861870.15331637858555835No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC813740.14475462646363402No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT800410.142383378680853No Hit
ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA798630.1420667379416669No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT778120.13841825391879828No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT766850.13641345553080558No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT763330.13578728957466235No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA736610.13103412072575693No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC732280.1302638654444785No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT707530.12586113606534638No Hit
GTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTAT695030.12363753536881504No Hit
CTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACGACT694640.12356815902708326No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA693660.1233938287324752No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC672300.11959413986224242No Hit
ATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAAT620700.11041511618696098No Hit
ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT592140.10533463331552616No Hit
CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT589120.10479741138724417No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT588100.1046159655704072No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG562780.1001118399995133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG729250.047.8021161
CGCGTGT421250.047.698521
GTTTTTT5971600.043.2951161
GATCGGA858750.041.348092
GCGTGTT500350.040.7342572
ATCGGAA883700.040.1968773
TATGCCG621200.039.27706546-47
TCGGAAG911800.039.106484
TGCCGTC615200.039.04641348-49
CGGAAGA929750.038.6579675
CCGTCTT658150.036.6137550-51
ATCTCGT690300.034.84314740-41
AGAGCAC1036100.034.767869
CTCGTAT705700.034.3418842-43
CGTCTGA722150.034.27643616-17
GAAGAGC1072650.033.5743067
CGTATGC738000.033.2474844-45
GATCATC746650.032.48066736-37
CACACGT783800.031.92275812-13
TCACCAG777850.031.8433530-31
Produced by FastQC (version 0.11.5)