##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233094_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56215129 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.556703107449955 33.0 31.0 34.0 30.0 34.0 2 32.336392183677106 34.0 31.0 34.0 30.0 34.0 3 32.1474772921005 34.0 31.0 34.0 30.0 34.0 4 35.755846188665686 37.0 35.0 37.0 35.0 37.0 5 35.86966496154443 37.0 35.0 37.0 35.0 37.0 6 35.976561007269055 37.0 35.0 37.0 35.0 37.0 7 36.02935161813824 37.0 35.0 37.0 35.0 37.0 8 36.03675869889047 37.0 35.0 37.0 35.0 37.0 9 37.80451831747998 39.0 38.0 39.0 35.0 39.0 10-11 37.69989057572028 39.0 37.0 39.0 35.0 39.0 12-13 37.68348488535889 39.0 37.0 39.0 35.0 39.0 14-15 39.079831872306116 40.0 38.0 41.0 36.0 41.0 16-17 38.95338858868402 40.0 38.0 41.0 35.5 41.0 18-19 38.742328920031476 40.0 38.0 41.0 34.5 41.0 20-21 38.585362314120104 40.0 38.0 41.0 34.0 41.0 22-23 38.38431993102782 40.0 38.0 41.0 34.0 41.0 24-25 38.05420069390929 40.0 38.0 41.0 33.5 41.0 26-27 37.60925336487264 40.0 38.0 41.0 33.0 41.0 28-29 37.26778675541241 40.0 38.0 41.0 32.0 41.0 30-31 37.083606514538104 40.0 38.0 41.0 31.5 41.0 32-33 37.05149260619859 40.0 38.0 41.0 31.0 41.0 34-35 36.95769968792565 40.0 38.0 41.0 31.0 41.0 36-37 36.859073577861935 40.0 38.0 41.0 30.5 41.0 38-39 36.73292056307476 40.0 38.0 41.0 30.0 41.0 40-41 36.6310748126185 40.0 37.0 41.0 30.0 41.0 42-43 36.481932986403 40.0 37.0 41.0 30.0 41.0 44-45 36.39713113528566 40.0 37.0 41.0 30.0 41.0 46-47 36.2588732385547 40.0 37.0 41.0 30.0 41.0 48-49 36.13480161185791 40.0 37.0 41.0 29.0 41.0 50-51 36.00124462046507 40.0 36.0 41.0 28.5 41.0 52-53 35.847607429665416 40.0 36.0 41.0 27.5 41.0 54-55 35.63233928539059 40.0 36.0 41.0 27.0 41.0 56-57 35.63707599069994 40.0 36.0 41.0 27.0 41.0 58-59 35.54278560847917 39.5 35.0 41.0 27.0 41.0 60-61 35.35895891122121 39.0 35.0 41.0 26.0 41.0 62-63 35.11772025818886 39.0 35.0 41.0 26.0 41.0 64-65 34.77852288660584 39.0 35.0 40.5 25.0 41.0 66-67 34.442938759421864 38.0 34.0 40.0 24.0 41.0 68-69 34.113522064496195 37.5 34.0 40.0 23.0 41.0 70-71 33.65476301762111 37.0 34.0 39.5 22.0 41.0 72-73 33.216225849094826 36.5 34.0 39.0 20.0 41.0 74-75 32.798509116647224 36.0 33.0 39.0 18.5 40.5 76-77 31.613054957145078 35.0 31.5 37.0 17.5 39.0 78-79 31.981341277363253 35.0 32.5 37.0 17.5 39.0 80-81 31.71726154893285 35.0 32.5 37.0 14.0 39.0 82-83 31.34968995623936 35.0 32.0 36.5 9.5 39.0 84-85 30.9652980605986 35.0 32.0 36.0 7.0 37.0 86-87 30.60624668316602 35.0 32.0 36.0 2.0 37.0 88-89 30.283100399894128 35.0 31.0 35.0 2.0 36.5 90-91 29.978110705749692 35.0 31.0 35.0 2.0 36.0 92-93 29.68466149032585 34.0 31.0 35.0 2.0 36.0 94-95 29.378969387404588 34.0 31.0 35.0 2.0 36.0 96-97 29.02608167989795 34.0 30.5 35.0 2.0 35.5 98-99 28.61328019900123 34.0 30.0 35.0 2.0 35.0 100-101 26.859834520703494 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8834562269228066 1101 2 0.4372022603469645 1101 3 0.42665045090539877 1101 4 0.41743119381049354 1101 5 0.2657813814444978 1101 6 0.1326365022571494 1101 7 0.1293466693688785 1101 8 0.1189080673359939 1101 9 0.13366973084310985 1101 10-11 0.1141815344599948 1101 12-13 0.11990367787952749 1101 14-15 0.1688260843654419 1101 16-17 0.17125125846744993 1101 18-19 0.18538195427717596 1101 20-21 0.1668909044272482 1101 22-23 0.14628871978820968 1101 24-25 0.14295520043850019 1101 26-27 0.15628866504236782 1101 28-29 0.1692770569887898 1101 30-31 0.16250382130643004 1101 32-33 -0.017417500984727496 1101 34-35 -3.7069287124325E-5 1101 36-37 0.07525328070043003 1101 38-39 -0.011993521186866474 1101 40-41 0.08897878493906575 1101 42-43 0.04527730851533107 1101 44-45 0.00609591591652503 1101 46-47 -0.05638924557306524 1101 48-49 -0.03392004379213631 1101 50-51 -0.07131299845860184 1101 52-53 -0.016787193026750913 1101 54-55 0.02440851926549925 1101 56-57 -0.06160585051718925 1101 58-59 0.0021967802736284625 1101 60-61 0.07892626301840977 1101 62-63 -0.010612019925801519 1101 64-65 0.011479131262895237 1101 66-67 -0.11993021794103953 1101 68-69 -0.04462814802833748 1101 70-71 -0.06251886113950178 1101 72-73 -0.0012912672154641314 1101 74-75 -0.07303552711945116 1101 76-77 -0.14829181565700722 1101 78-79 -0.09573615175560235 1101 80-81 -0.010928604009052378 1101 82-83 0.03164316982826065 1101 84-85 0.15010720497995322 1101 86-87 0.05171393006051517 1101 88-89 0.0783530229626841 1101 90-91 0.11495329321200032 1101 92-93 0.13137955474870822 1101 94-95 0.16623281157506042 1101 96-97 0.135113391467641 1101 98-99 0.14842573288765237 1101 100-101 0.2845587182605023 1102 1 0.8690687240133208 1102 2 0.41635690479000687 1102 3 0.439677255055976 1102 4 0.3924420064576779 1102 5 0.2320016897638908 1102 6 0.05430332247200198 1102 7 0.11323957424866649 1102 8 0.11119098628086732 1102 9 0.1066825716512767 1102 10-11 0.06512885658042222 1102 12-13 0.10146700874615533 1102 14-15 0.13897064150494742 1102 16-17 0.1651805668856312 1102 18-19 0.15686657462427434 1102 20-21 0.1768571221701194 1102 22-23 0.11482230172292418 1102 24-25 0.029651277300480672 1102 26-27 0.09043305574731164 1102 28-29 0.10386706432519333 1102 30-31 0.08293953522339592 1102 32-33 -0.018196831767944843 1102 34-35 -0.035092607595125 1102 36-37 -0.047077436288532226 1102 38-39 -0.0349740829643963 1102 40-41 -0.01912486096995991 1102 42-43 0.007938727130543555 1102 44-45 0.015616503226738132 1102 46-47 -0.014459204334926312 1102 48-49 -0.05946318954699592 1102 50-51 -0.10787409171727091 1102 52-53 -0.04598272292753336 1102 54-55 0.03119890310488671 1102 56-57 -0.029425266194017752 1102 58-59 -0.005583943626184862 1102 60-61 0.060509800416255644 1102 62-63 0.08560518288744845 1102 64-65 0.03321030449264839 1102 66-67 0.02210192732049876 1102 68-69 0.09311728754875048 1102 70-71 -0.02581586435682226 1102 72-73 0.018911447778300783 1102 74-75 0.043870359261866554 1102 76-77 0.05454867200446145 1102 78-79 0.11954267787062633 1102 80-81 0.1489818762153874 1102 82-83 0.11553089018942586 1102 84-85 0.145582361932302 1102 86-87 0.1593571167410559 1102 88-89 0.10837803798903778 1102 90-91 0.0949428060364923 1102 92-93 0.11791323370189488 1102 94-95 0.12619108929154166 1102 96-97 0.1242768473052962 1102 98-99 0.2007616605780811 1102 100-101 0.31215931625773763 1103 1 0.8441634140326038 1103 2 0.3806803180188041 1103 3 0.4076117299089148 1103 4 0.3780001973687064 1103 5 0.20326076192215936 1103 6 0.13874275388138813 1103 7 0.06628803382768922 1103 8 0.09144551206595253 1103 9 0.1390989066067405 1103 10-11 0.09112004639805349 1103 12-13 0.12415962968827898 1103 14-15 0.117997305670265 1103 16-17 0.15297402492691958 1103 18-19 0.1480578035931046 1103 20-21 0.18346439659667624 1103 22-23 0.09592449696409489 1103 24-25 0.08949417680163663 1103 26-27 0.15224420652496207 1103 28-29 0.0951355260086828 1103 30-31 0.15129582127399743 1103 32-33 0.045623100853127596 1103 34-35 0.1101444614191962 1103 36-37 0.07925472470459027 1103 38-39 -0.05465105080624255 1103 40-41 0.07111218490532423 1103 42-43 0.0724275687844127 1103 44-45 0.0687285872322363 1103 46-47 0.09057130484529097 1103 48-49 -0.0014686071623302155 1103 50-51 -0.09704536234468009 1103 52-53 -0.07469444769372302 1103 54-55 -0.015545243130034692 1103 56-57 0.012375467586302591 1103 58-59 0.08686063819045131 1103 60-61 0.07140640708923485 1103 62-63 0.05906918744688028 1103 64-65 0.15169102215457286 1103 66-67 0.12078023411265093 1103 68-69 0.12680808331000293 1103 70-71 0.10949817838864107 1103 72-73 0.07591657752614367 1103 74-75 0.09028538076776016 1103 76-77 0.07899272191485451 1103 78-79 0.09734138898350153 1103 80-81 0.060226309840505365 1103 82-83 0.08856749066085357 1103 84-85 0.2233361712797084 1103 86-87 0.25963853178553364 1103 88-89 0.30790628455619284 1103 90-91 0.2981516974744842 1103 92-93 0.3293823346376499 1103 94-95 0.3421983689237429 1103 96-97 0.34337630878068026 1103 98-99 0.3448091372244697 1103 100-101 0.36385809765958044 1104 1 0.7614919904454887 1104 2 0.2950433014819822 1104 3 0.34656012386665935 1104 4 0.3227256437483135 1104 5 0.16148519358594626 1104 6 0.10142513427555144 1104 7 0.06225262301848744 1104 8 0.033491880017344045 1104 9 0.025006321541020782 1104 10-11 -0.06974152633028297 1104 12-13 -0.03639207679997725 1104 14-15 -0.06747444248986056 1104 16-17 -0.041430592800459465 1104 18-19 -0.04087812690129056 1104 20-21 0.03931200352955955 1104 22-23 -0.04911984257267221 1104 24-25 -0.11394027901732784 1104 26-27 -0.060766292818762224 1104 28-29 -3.5861335521047977E-4 1104 30-31 -0.044076651080636964 1104 32-33 -0.13823228308519475 1104 34-35 -0.11076448751314416 1104 36-37 -0.08627839270042159 1104 38-39 -0.12329695201179192 1104 40-41 -0.09796530791201263 1104 42-43 -0.014511671407504423 1104 44-45 -0.0391782316847511 1104 46-47 -0.02923015201879764 1104 48-49 -0.08484001736672298 1104 50-51 -0.23105188025842693 1104 52-53 -0.21302810657584104 1104 54-55 -0.1006007988881592 1104 56-57 -0.1681049145267437 1104 58-59 -0.055099189622772826 1104 60-61 -0.05024150532654659 1104 62-63 -0.04776612225545307 1104 64-65 0.00843727240663128 1104 66-67 0.047817134356428426 1104 68-69 -0.09844127605287412 1104 70-71 -0.06238905326819122 1104 72-73 -0.04234302296035963 1104 74-75 0.007920431657510107 1104 76-77 0.025066472083576485 1104 78-79 -0.007352479231180098 1104 80-81 0.020692433873982452 1104 82-83 0.12643555015511154 1104 84-85 -0.00334272237648392 1104 86-87 -0.028393617761189205 1104 88-89 8.289872164368717E-4 1104 90-91 0.025084621270661245 1104 92-93 0.021032194798831227 1104 94-95 0.013838010341274298 1104 96-97 0.07184216161948953 1104 98-99 0.03933073736385495 1104 100-101 0.06284515255583756 1105 1 0.7298774924808704 1105 2 0.28081749515968824 1105 3 0.317577441952821 1105 4 0.31179377845229794 1105 5 0.0827840987385855 1105 6 0.01987936760730946 1105 7 1.4432843461520406E-4 1105 8 -0.06687750591692065 1105 9 -0.017050363859986817 1105 10-11 -0.1251642530474797 1105 12-13 -0.07264882628006575 1105 14-15 -0.15082190690072395 1105 16-17 -0.10214063062345957 1105 18-19 -0.12817739055711286 1105 20-21 -0.0686299253405167 1105 22-23 -0.08612737065240594 1105 24-25 -0.11660757480072448 1105 26-27 -0.11507770308504206 1105 28-29 -0.14683188747984133 1105 30-31 -0.18392557190636438 1105 32-33 -0.24743279154817088 1105 34-35 -0.19394044012266676 1105 36-37 -0.1885133200803466 1105 38-39 -0.23902635968652675 1105 40-41 -0.19892191227803124 1105 42-43 -0.16147806522524633 1105 44-45 -0.15034374060041245 1105 46-47 -0.16645549106517166 1105 48-49 -0.15939615463891954 1105 50-51 -0.1712758448868854 1105 52-53 -0.16331121758832268 1105 54-55 -0.3497924071197005 1105 56-57 -0.23602779932688378 1105 58-59 -0.25207869143793715 1105 60-61 -0.24821634331043896 1105 62-63 -0.25491552476252366 1105 64-65 -0.18796014334662203 1105 66-67 -0.1570666834002381 1105 68-69 -0.20757566401704963 1105 70-71 -0.28089347627967953 1105 72-73 -0.25708577687130685 1105 74-75 -0.2010022208704143 1105 76-77 -0.13500488767294883 1105 78-79 -0.06718907576405542 1105 80-81 -0.06362814212826606 1105 82-83 -0.05390629370316802 1105 84-85 -0.10946512085428495 1105 86-87 -0.13610173934070247 1105 88-89 -0.14821889495324214 1105 90-91 -0.1255950671277084 1105 92-93 -0.12575246152535513 1105 94-95 -0.03685903794046297 1105 96-97 -0.03987327484971104 1105 98-99 -0.1462815883244879 1105 100-101 -0.1577672402886776 1106 1 0.6525376240580947 1106 2 0.22123434249339624 1106 3 0.24457838540767085 1106 4 0.248428965295588 1106 5 0.10257603964783613 1106 6 -0.03563220460700478 1106 7 -0.014451137765973954 1106 8 -0.07321059738183067 1106 9 -0.11104554379364373 1106 10-11 -0.14265432210359563 1106 12-13 -0.1956523005067865 1106 14-15 -0.2191465275896789 1106 16-17 -0.19838817687086419 1106 18-19 -0.20811105330745505 1106 20-21 -0.21520735067522878 1106 22-23 -0.24369772748745788 1106 24-25 -0.22379120209583903 1106 26-27 -0.18274366288711263 1106 28-29 -0.23458279129889803 1106 30-31 -0.15708989346626367 1106 32-33 -0.2357142189396555 1106 34-35 -0.20574064382630297 1106 36-37 -0.17732506696697214 1106 38-39 -0.218743470897941 1106 40-41 -0.20789295913204597 1106 42-43 -0.20394799918485518 1106 44-45 -0.2611848473112346 1106 46-47 -0.31931658663626905 1106 48-49 -0.24992321961001807 1106 50-51 -0.2138091137914344 1106 52-53 -0.20147907782929764 1106 54-55 -0.2538629995245074 1106 56-57 -0.25233658556563654 1106 58-59 -0.23559260031591833 1106 60-61 -0.2695932805246386 1106 62-63 -0.3672442708890813 1106 64-65 -0.35273738200606175 1106 66-67 -0.3710630936215793 1106 68-69 -0.31122576427579673 1106 70-71 -0.19466256837808515 1106 72-73 -0.21578634882693848 1106 74-75 -0.22458069498795652 1106 76-77 -0.2131602480024135 1106 78-79 -0.0807422394621895 1106 80-81 -0.13509251587254312 1106 82-83 -0.15706746919708436 1106 84-85 -0.13304232684640382 1106 86-87 -0.1246098474857611 1106 88-89 -0.1519615498097373 1106 90-91 -0.17233850225199987 1106 92-93 -0.26084975611109584 1106 94-95 -0.2788639914901232 1106 96-97 -0.19070229385470228 1106 98-99 -0.15248699730350523 1106 100-101 -0.17640040747713925 1107 1 0.6184628273141577 1107 2 0.1422872595361966 1107 3 0.20696525282725986 1107 4 0.23346959151709967 1107 5 -0.005721340889671467 1107 6 -0.043312858935820486 1107 7 -0.11345469674015618 1107 8 -0.0943089765736147 1107 9 -0.14204791766660918 1107 10-11 -0.2331808022057018 1107 12-13 -0.2124217043290102 1107 14-15 -0.24624911215129686 1107 16-17 -0.25286196271392214 1107 18-19 -0.26729202493519466 1107 20-21 -0.23990079707052558 1107 22-23 -0.2717583140284745 1107 24-25 -0.3157645699265075 1107 26-27 -0.2758221869816353 1107 28-29 -0.2272043573815452 1107 30-31 -0.2085642872293434 1107 32-33 -0.27064457762184446 1107 34-35 -0.26135010025110006 1107 36-37 -0.24785393706119407 1107 38-39 -0.3013858015030664 1107 40-41 -0.276522649891497 1107 42-43 -0.30665069436987125 1107 44-45 -0.30718980259123896 1107 46-47 -0.3001823950907436 1107 48-49 -0.3880894751767201 1107 50-51 -0.325840581398829 1107 52-53 -0.25770266231703687 1107 54-55 -0.29926244796215684 1107 56-57 -0.30096987746625814 1107 58-59 -0.29191243308118686 1107 60-61 -0.3318010958595252 1107 62-63 -0.23781001601393825 1107 64-65 -0.21653945060543123 1107 66-67 -0.24745030834221637 1107 68-69 -0.2060812630911144 1107 70-71 -0.32211152022222933 1107 72-73 -0.2682710218235371 1107 74-75 -0.30802921937784333 1107 76-77 -0.29862119904449713 1107 78-79 -0.17476860125566773 1107 80-81 -0.1213737947632616 1107 82-83 -0.22700031012957922 1107 84-85 -0.20866986566798218 1107 86-87 -0.2623671355690007 1107 88-89 -0.16015764665732135 1107 90-91 -0.1775577723990267 1107 92-93 -0.24011215811208686 1107 94-95 -0.20173574137196582 1107 96-97 -0.21818544539818774 1107 98-99 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62-63 -1.1395486011299099 2316 64-65 -1.1636459676909894 2316 66-67 -1.0430704970667364 2316 68-69 -1.1128619956922492 2316 70-71 -1.1230747988413512 2316 72-73 -1.0905452187462288 2316 74-75 -1.1498543865063162 2316 76-77 -1.13955181814935 2316 78-79 -1.1357896202306357 2316 80-81 -1.0980149101908694 2316 82-83 -1.0510612462528144 2316 84-85 -1.1680260482871425 2316 86-87 -1.3436241055233324 2316 88-89 -1.3727468829751892 2316 90-91 -1.3739174706478927 2316 92-93 -1.5644933610394922 2316 94-95 -1.5738876891625075 2316 96-97 -1.550929762696935 2316 98-99 -1.665336758931769 2316 100-101 -1.8121103036662056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6216.0 3 1094.0 4 2883.0 5 7022.0 6 20039.0 7 63816.0 8 215436.0 9 483961.0 10 741382.0 11 586464.0 12 355018.0 13 243832.0 14 203908.0 15 181181.0 16 168476.0 17 165956.0 18 170432.0 19 181628.0 20 196736.0 21 215043.0 22 239854.0 23 274446.0 24 319745.0 25 379445.0 26 462580.0 27 582282.0 28 629747.0 29 724269.0 30 893669.0 31 1110225.0 32 1393155.0 33 1781450.0 34 2371609.0 35 3421504.0 36 5453758.0 37 1.0257163E7 38 1.7083818E7 39 4615667.0 40 10220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.825629415057477 21.660120453456546 10.252028451925485 43.26222167956049 2 14.282948634699387 15.75132736954139 59.265280704061 10.70044329169822 3 14.581908874709782 26.119169854155206 37.1846437066504 22.114277564484613 4 18.46370751902037 32.26418639010861 31.47429760412006 17.79780848675096 5 18.371058082958417 31.919241882376543 33.59107652319005 16.118623511474997 6 14.330471428785657 38.454384761796064 32.75602907537578 14.459114734042503 7 12.073973894109539 21.720748875271635 50.59054476242507 15.614732468193749 8 12.57865831811931 22.109930584700784 46.56541835917516 18.745992738004745 9 14.251777310695132 24.525486724401183 40.03959681387549 21.18313915102819 10-11 20.877390124307933 32.484704993994995 29.672988518281162 16.96491636341591 12-13 18.12697432394045 26.2864210451247 37.69436338036331 17.892241250571534 14-15 17.848603531622242 28.603744732134295 36.816212767207205 16.73143896903625 16-17 19.47256404943943 27.637906870230612 36.144780526964546 16.74474855336541 18-19 19.312138374706922 29.690568707936254 35.47143332180204 15.525859595554783 20-21 19.65963023939694 29.138031507496855 34.00461911241011 17.197719140696094 22-23 19.84208823927096 29.464731816234025 33.62019324015071 17.072986704344306 24-25 17.566907122102307 29.980648981522396 33.911572096543615 18.540871799831677 26-27 17.209202704133258 29.105633645348387 34.65679586006109 19.028367790457263 28-29 17.073026729156844 31.691638562280982 32.359616216481506 18.875718492080665 30-31 17.49377378463367 30.60791428584999 33.283486728279144 18.6148252012372 32-33 18.800199675784786 30.440334842956602 31.223815211737747 19.53565026952086 34-35 19.260193283555395 30.881664435920804 30.775822821646464 19.082319458877343 36-37 19.939768349548746 30.284574282485416 31.787657198118325 17.988000169847513 38-39 19.83187746487249 30.278054685243184 31.904572343861382 17.985495506022943 40-41 19.85799409977339 29.84991904937192 32.50086555880713 17.791221292047553 42-43 19.788725380315324 30.389009691679263 31.832068730110002 17.99019619789541 44-45 19.948063269587088 29.7237119210382 31.95436054233728 18.373864267037437 46-47 19.432868329804954 29.828813521000725 32.25189254657763 18.48642560261669 48-49 18.22840609331342 30.83300849491958 31.956542339340714 18.982043072426286 50-51 17.670875575149886 30.24706925425716 32.70136318641197 19.380691984180984 52-53 18.414252860648954 30.43441650734271 31.782711910169237 19.3686187218391 54-55 18.227950699890773 31.029386235153883 31.106019520118867 19.63664354483648 56-57 18.993505289296767 30.50019862090862 31.74948864744222 18.756807442352397 58-59 19.577777718877066 29.394398436762458 31.54020957596664 19.487614268393834 60-61 19.95178735603364 30.828283788159588 29.810229555819394 19.409699299987377 62-63 19.466528307708767 30.377939717971653 31.885583683353286 18.269948290966298 64-65 20.26998283682672 29.728435738357906 31.47777709448999 18.523804330325383 66-67 19.299004010112654 32.71207738400814 29.234314307096938 18.754604298782272 68-69 19.639597376001753 30.984736333167533 31.715458662382506 17.66020762844821 70-71 19.72071699773205 30.86500521950239 30.571433892822697 18.842843889942866 72-73 18.019317362057464 33.41141759187282 29.905507287904648 18.66375775816507 74-75 18.108353002267414 30.597042657324508 32.167321007126034 19.12728333328204 76-77 18.549708388999694 31.561445852058796 30.86814405424561 19.020701704695902 78-79 18.442066547601446 32.09244881391271 30.201639313146465 19.263845325339375 80-81 18.8762699450534 30.726717713304545 31.22884766483414 19.168164676807912 82-83 19.457585875147597 31.03804315738562 30.393276336695767 19.111094630771017 84-85 19.157167637203145 31.081285964851208 30.644168761046515 19.117377636899135 86-87 19.594915454165374 30.81422084791445 30.39947484599742 19.191388851922763 88-89 19.438747530046584 30.99997333458045 30.577905460289877 18.98337367508309 90-91 19.540148342807758 31.12806724261068 30.904274282678195 18.42751013190337 92-93 19.13636807628779 31.88801541307501 30.13338277672546 18.842233733911737 94-95 19.233847836811528 31.122486213725182 30.88133741819503 18.762328531268256 96-97 18.46258788945082 31.665984784604817 29.983743877092518 19.887683448851845 98-99 18.45492123447195 31.556916564994182 31.21376980945759 18.774392391076276 100-101 18.43157989423507 32.1798485165707 29.968619756148897 19.41995183304534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28758.0 1 36169.5 2 36250.0 3 33351.5 4 42414.5 5 53270.5 6 63812.0 7 74440.5 8 86260.0 9 95194.5 10 101112.5 11 106934.5 12 114654.0 13 121233.5 14 130378.5 15 141759.0 16 149972.5 17 158322.5 18 177015.0 19 199532.5 20 229126.5 21 272846.5 22 316210.5 23 369783.5 24 442505.5 25 529393.5 26 649418.0 27 800576.0 28 1239013.5 29 1533020.5 30 1683279.5 31 2027137.0 32 2526754.0 33 2731616.5 34 2447990.0 35 2631543.0 36 2955786.5 37 3136730.5 38 3329423.5 39 2820335.5 40 2402739.5 41 2170493.5 42 1687661.5 43 1505039.5 44 1371584.0 45 1251965.0 46 1163694.0 47 1097124.0 48 1064823.5 49 1022127.0 50 939358.5 51 863896.0 52 815984.5 53 741137.0 54 641288.0 55 544273.0 56 463290.0 57 396538.0 58 336545.5 59 277039.0 60 212040.5 61 161515.0 62 117174.0 63 79084.5 64 51388.5 65 34645.0 66 25905.0 67 19780.5 68 14755.0 69 11087.0 70 10847.5 71 13515.5 72 13116.0 73 18393.5 74 19402.5 75 11243.0 76 8022.5 77 8258.5 78 7649.0 79 4398.5 80 2751.5 81 1768.0 82 1335.5 83 887.5 84 371.5 85 115.0 86 44.0 87 21.0 88 13.0 89 9.5 90 5.0 91 1.5 92 3.5 93 3.0 94 0.5 95 1.5 96 3.0 97 2.5 98 4.0 99 5.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08457153945159496 2 0.0 3 0.41767581819477817 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.670802089594067E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.894402786125422E-7 92-93 0.0 94-95 4.473884601421087E-4 96-97 0.0010993481843651022 98-99 0.0020172505518932455 100-101 0.07281313896833716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.6215129E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.304949169351534 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95077570618346 12.970193637989727 2 9.672025832686392 3.347478307985859 3 3.781625941429873 1.963225340818353 4 2.0918222375180555 1.4479550996627657 5 1.4459588534327572 1.2511122229813845 6 1.0142271814466755 1.0530689892665634 7 0.7363202564369122 0.8919389227003256 8 0.6047593048675336 0.8372263224340029 9 0.48228819611110624 0.7511375446812845 >10 4.000919044434809 14.316206833069039 >50 0.6106672523263985 7.445154871478329 >100 0.5187257342360094 17.8891963204678 >500 0.053699676781831476 6.3889495981562625 >1k 0.03063244211764081 10.041844101604083 >5k 0.0028385559565139006 3.3382154397087485 >10k+ 0.0027137840340126854 16.067096446995414 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG 327569 0.5827061252496637 TruSeq Adapter, Index 7 (100% over 49bp) ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 221388 0.393822808802947 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 180818 0.3216536245963253 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 180818 0.3216536245963253 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 160883 0.28619164068804326 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 159779 0.28422775655286675 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 154093 0.2741130417044849 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 132070 0.23493675519271692 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 118767 0.2112723071399516 TruSeq Adapter, Index 7 (100% over 50bp) ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 116416 0.20709015894991545 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 113057 0.20111489915819636 No Hit CTCACTATAGGAATTTAATACGACTCACTATAGGAATTTAATACGACTCA 106443 0.18934938315270966 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 99800 0.1775322796110634 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 98004 0.17433741013028717 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 98003 0.17433563124972995 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 93339 0.16603893233083214 No Hit ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA 91967 0.16359830820631935 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 91051 0.16196885361590116 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 91034 0.16193861264642834 No Hit CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT 88142 0.1567940900749334 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 87951 0.1564543238885034 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 86187 0.15331637858555835 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 81374 0.14475462646363402 No Hit ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT 80041 0.142383378680853 No Hit ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA 79863 0.1420667379416669 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 77812 0.13841825391879828 No Hit CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT 76685 0.13641345553080558 No Hit CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT 76333 0.13578728957466235 No Hit ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA 73661 0.13103412072575693 No Hit CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC 73228 0.1302638654444785 No Hit ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT 70753 0.12586113606534638 No Hit GTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTAT 69503 0.12363753536881504 No Hit CTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAATACGACT 69464 0.12356815902708326 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 69366 0.1233938287324752 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 67230 0.11959413986224242 No Hit ATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAAT 62070 0.11041511618696098 No Hit ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT 59214 0.10533463331552616 No Hit CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT 58912 0.10479741138724417 No Hit GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT 58810 0.1046159655704072 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 56278 0.1001118399995133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.6475552159633041 0.0 0.0 0.0 0.0 2 0.6496827571097453 1.7788805572250843E-6 0.0 0.0 0.0 3 0.6498446352404528 5.336641671675253E-6 0.0 1.7788805572250843E-6 0.0 4 0.6575969967088398 5.336641671675253E-6 0.0 1.7788805572250843E-6 0.0 5 0.6582996545289436 5.336641671675253E-6 0.0 5.336641671675253E-6 0.0 6 0.6586910082515331 5.336641671675253E-6 0.0 5.336641671675253E-6 0.0 7 0.6600180531472231 5.336641671675253E-6 0.0 5.336641671675253E-6 0.0 8 0.6734966311293176 5.336641671675253E-6 0.0 8.894402786125422E-6 0.0 9 0.6740551996242862 7.115522228900337E-6 0.0 8.894402786125422E-6 0.0 10-11 0.6769423227686625 8.894402786125422E-6 0.0 8.894402786125422E-6 0.0 12-13 0.6795430461433256 8.894402786125422E-6 0.0 1.0673283343350506E-5 0.0 14-15 0.6815620755757761 8.894402786125422E-6 0.0 1.245216390057559E-5 0.0 16-17 0.6840195990655824 8.894402786125422E-6 0.0 1.4231044457800674E-5 0.0 18-19 0.686607870276345 8.894402786125422E-6 0.0 1.4231044457800674E-5 0.0 20-21 0.6891721265995849 8.894402786125422E-6 0.0 1.4231044457800674E-5 0.0 22-23 0.6917532822881185 8.894402786125422E-6 0.0 1.4231044457800674E-5 0.0 24-25 0.6937643067580614 9.783843064737963E-6 0.0 1.4231044457800674E-5 0.0 26-27 0.6955120569055351 1.0673283343350506E-5 0.0 1.600992501502576E-5 0.0 28-29 0.6973514194017059 1.0673283343350506E-5 0.0 1.7788805572250843E-5 0.0 30-31 0.6993660016327633 1.0673283343350506E-5 0.0 1.7788805572250843E-5 0.0 32-33 0.701568255762608 1.0673283343350506E-5 0.0 1.7788805572250843E-5 0.0 34-35 0.7039154886578665 1.0673283343350506E-5 0.0 2.3125447243926098E-5 0.0 36-37 0.7064317152060613 1.0673283343350506E-5 0.0 2.490432780115118E-5 0.0 38-39 0.7091925378308747 1.245216390057559E-5 0.0 2.490432780115118E-5 0.0 40-41 0.7120218473571411 1.3341604179188131E-5 0.0 2.490432780115118E-5 0.0 42-43 0.7152211640393105 1.600992501502576E-5 0.0 2.846208891560135E-5 0.0 44-45 0.7186526246341977 1.600992501502576E-5 0.0 2.846208891560135E-5 0.0 46-47 0.7224905594364108 1.600992501502576E-5 0.0 2.9351529194213892E-5 0.0 48-49 0.7261274807356575 1.7788805572250846E-5 0.0 3.0240969472826435E-5 0.0 50-51 0.7303843419090971 1.956768612947593E-5 0.0 3.201985003005152E-5 0.0 52-53 0.7349400550161505 1.956768612947593E-5 0.0 3.8245931980339315E-5 0.0 54-55 0.7398897901666293 1.956768612947593E-5 0.0 4.091425281617694E-5 0.0 56-57 0.7456489159706456 2.045712640808847E-5 0.0 4.536145420923965E-5 0.0 58-59 0.7517887222139079 2.134656668670101E-5 0.0 4.802977504507728E-5 0.0 60-61 0.7583696908353621 2.134656668670101E-5 0.0 4.980865560230236E-5 0.0 62-63 0.765920149360504 2.9351529194213892E-5 0.0 5.0698095880914903E-5 0.0 64-65 0.7746384429714641 3.37987305872766E-5 0.0 5.8703058388427784E-5 0.0 66-67 0.7841919210040416 3.37987305872766E-5 0.0 6.759746117455321E-5 0.0 68-69 0.7952343220630161 3.37987305872766E-5 0.0 7.649186396067863E-5 0.0 70-71 0.8071314752297376 3.37987305872766E-5 0.0 7.827074451790372E-5 0.0 72-73 0.8202249255711928 3.5577611144501686E-5 0.0 8.182850563235387E-5 0.0 74-75 0.8359849178679284 3.735649170172677E-5 0.0 8.716514730402913E-5 0.0 76-77 0.853087964985369 3.735649170172677E-5 0.0 9.339122925431693E-5 0.0 78-79 0.8722073732144242 3.735649170172677E-5 0.0 9.428066953292948E-5 0.0 80-81 0.8949361300940891 3.735649170172677E-5 0.0 9.69489903687671E-5 0.0 82-83 0.9212439946548909 3.913537225895186E-5 0.0 9.783843064737963E-5 0.0 84-85 0.9512323630885913 3.913537225895186E-5 0.0 9.783843064737963E-5 0.0 86-87 0.9880783160703945 4.180369309478948E-5 0.0 1.0406451259766744E-4 0.0 88-89 1.0314972327111445 4.269313337340202E-5 0.0 1.0495395287627998E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 72925 0.0 47.802116 1 CGCGTGT 42125 0.0 47.69852 1 GTTTTTT 597160 0.0 43.295116 1 GATCGGA 85875 0.0 41.34809 2 GCGTGTT 50035 0.0 40.734257 2 ATCGGAA 88370 0.0 40.196877 3 TATGCCG 62120 0.0 39.277065 46-47 TCGGAAG 91180 0.0 39.10648 4 TGCCGTC 61520 0.0 39.046413 48-49 CGGAAGA 92975 0.0 38.657967 5 CCGTCTT 65815 0.0 36.61375 50-51 ATCTCGT 69030 0.0 34.843147 40-41 AGAGCAC 103610 0.0 34.76786 9 CTCGTAT 70570 0.0 34.34188 42-43 CGTCTGA 72215 0.0 34.276436 16-17 GAAGAGC 107265 0.0 33.574306 7 CGTATGC 73800 0.0 33.24748 44-45 GATCATC 74665 0.0 32.480667 36-37 CACACGT 78380 0.0 31.922758 12-13 TCACCAG 77785 0.0 31.84335 30-31 >>END_MODULE