FastQCFastQC Report
Fri 27 May 2016
SRR1233093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45001521
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1363320.30294976029810194No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1134920.25219592022234094Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG477500.10610752467677703Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT8511300.048.8403471
CGCGTGT259250.048.6405531
CGCCGTA262950.037.27483446-47
GCGTGTT362150.035.554992
CCGTATC280500.035.19031548-49
GTCGCCG283900.033.68601644-45
AGATCGG377600.032.3001631
GATCTCG326350.031.65622334-35
TCTCGGT301800.031.4954736-37
TGGTCGC310300.029.36458642-43
GTAGGGA334750.029.0510718-19
AGAGCGT414100.029.0072949
AGCGTCG341350.028.86630410-11
GATCGGA437100.027.979442
ATCGGAA449850.027.1919543
CGTCGTG347200.027.13705412-13
GTGTAGG353900.026.83039316-17
TCGGTGG368850.026.69159738-39
GGTGGTC345050.026.63376840-41
CGTGTTT519250.025.2311733
Produced by FastQC (version 0.11.5)