FastQCFastQC Report
Fri 27 May 2016
SRR1233093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45001521
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1254890.2788550191447974TruSeq Adapter, Index 3 (100% over 49bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1251400.2780794898021336No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1146190.25470028001942424TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT251800.051.5231631
GTTTTTT7017450.047.4266241
GCGTGTT345750.038.531562
AGATCGG378100.032.87321
TATGCCG376600.032.3739246-47
TGCCGTC395750.030.69338648-49
CCGTCTT415450.029.22652450-51
CACGTCT431900.028.48167814-15
GCATCTC429150.028.07220538-39
CGTCTGA443900.027.73847416-17
CACACGT445150.027.68191712-13
CGTGTTT499650.027.3493543
GATCGGA466700.027.0357972
AGGCATC454750.026.99311436-37
CGTATGC460900.026.57111744-45
ATCTCGT457950.026.44157440-41
CCAGTCA465650.026.27967526-27
ACTTAGG465800.026.16926832-33
ATCGGAA484350.026.0505963
CTCGTAT484450.025.07850342-43
Produced by FastQC (version 0.11.5)