FastQCFastQC Report
Fri 27 May 2016
SRR1233092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34455644
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1003900.29136010344197893Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT848950.2463892417741488No Hit
CAAGCAGAAGACGGCATACGAGATTGGAAGAGCGTCGTGTAGGGAAAGAG519700.1508316025090113Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG397490.11536281254821416Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC162050.067.201679
AAGACGG173400.063.3284078
GTTTTTT5986400.050.080441
CGCGTGT189900.049.8931
GAAGACG276400.040.0211757
CGGCATA143600.037.95736312-13
GCGTGTT252750.037.62832
ACGGCAT151850.035.7151310-11
CAAGCAG329150.034.712961
GACGGCA161500.033.86017610-11
ATACGAG165900.032.64959716-17
TACGAGA164950.032.30544716-17
AGATCGG394550.029.2002181
GCATACG190050.029.05692714-15
GCAGAAG408350.027.7350714
GGCATAC204450.026.96183412-13
AGAAGAC424450.026.6215696
CGTGTTT370600.026.2528323
GATCGGA447100.025.8321362
ACGAGAT213500.025.82763718-19
Produced by FastQC (version 0.11.5)