##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233092_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34455644 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.185820500118936 34.0 31.0 34.0 31.0 34.0 2 32.37027753711409 34.0 33.0 34.0 31.0 34.0 3 32.40843732306963 34.0 33.0 34.0 31.0 34.0 4 35.642459969693206 37.0 37.0 37.0 35.0 37.0 5 35.61789644680564 37.0 37.0 37.0 35.0 37.0 6 35.640215954169946 37.0 37.0 37.0 35.0 37.0 7 35.6820498261475 37.0 37.0 37.0 35.0 37.0 8 35.65646353903587 37.0 37.0 37.0 35.0 37.0 9 37.40434620812776 39.0 39.0 39.0 35.0 39.0 10-11 37.36227886206393 39.0 39.0 39.0 35.0 39.0 12-13 37.32915729277909 39.0 38.5 39.0 35.0 39.0 14-15 38.77970375477527 41.0 39.0 41.0 36.0 41.0 16-17 38.574954381929416 41.0 39.0 41.0 35.5 41.0 18-19 38.07119673920476 41.0 38.0 41.0 34.0 41.0 20-21 37.70303051076334 40.5 38.0 41.0 33.5 41.0 22-23 37.24922355246066 40.0 38.0 41.0 32.0 41.0 24-25 36.70646768349475 40.0 37.5 41.0 30.5 41.0 26-27 36.19264041328033 40.0 37.5 41.0 26.0 41.0 28-29 35.8525589160371 40.0 38.0 41.0 21.5 41.0 30-31 35.53891876175642 40.0 37.5 41.0 17.0 41.0 32-33 35.35931423890959 40.0 37.5 41.0 14.5 41.0 34-35 35.177678466842764 40.0 37.0 41.0 9.5 41.0 36-37 35.04427328364549 40.0 37.0 41.0 9.0 41.0 38-39 34.895971484381484 40.0 37.0 41.0 7.5 41.0 40-41 34.77260313288586 40.0 37.0 41.0 7.0 41.0 42-43 34.64233520058426 40.0 36.0 41.0 2.0 41.0 44-45 34.34767986051864 40.0 35.5 41.0 2.0 41.0 46-47 34.2594961800743 40.0 35.0 41.0 2.0 41.0 48-49 34.16221248106696 40.0 35.0 41.0 2.0 41.0 50-51 33.645194833682396 39.5 34.5 40.5 2.0 41.0 52-53 33.68882993450942 39.5 35.0 40.5 2.0 41.0 54-55 34.00251533536857 40.0 35.0 41.0 2.0 41.0 56-57 33.725498223164834 40.0 35.0 41.0 2.0 41.0 58-59 33.56409871485786 40.0 35.0 41.0 2.0 41.0 60-61 33.549638602024096 39.5 35.0 41.0 2.0 41.0 62-63 33.36696003998648 39.0 35.0 41.0 2.0 41.0 64-65 33.08346985184778 39.0 34.5 41.0 2.0 41.0 66-67 32.70847838455725 38.5 34.0 41.0 2.0 41.0 68-69 32.37904904636233 37.5 34.0 40.5 2.0 41.0 70-71 32.0239616476186 37.0 34.0 40.0 2.0 41.0 72-73 31.641426858833345 36.5 33.0 39.5 2.0 41.0 74-75 31.265090415956237 36.0 33.0 39.0 2.0 41.0 76-77 30.887573527866728 36.0 33.0 39.0 2.0 40.5 78-79 30.414743198530843 35.0 32.0 37.5 2.0 39.5 80-81 30.052493098082856 35.0 32.0 37.0 2.0 39.0 82-83 29.749502737490555 35.0 32.0 36.5 2.0 39.0 84-85 29.406650431493894 35.0 31.0 36.0 2.0 37.5 86-87 29.06690213075106 35.0 31.0 36.0 2.0 37.0 88-89 28.78243340916803 35.0 31.0 35.5 2.0 37.0 90-91 28.50839218097331 35.0 30.5 35.0 2.0 36.0 92-93 28.258161768794686 35.0 30.0 35.0 2.0 36.0 94-95 28.0060752020772 35.0 30.0 35.0 2.0 36.0 96-97 27.759427236362207 35.0 29.0 35.0 2.0 36.0 98-99 27.49282981040784 35.0 29.0 35.0 2.0 35.5 100-101 25.971519658724127 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35915808320655884 1101 2 0.33056232626763205 1101 3 0.3849087256787911 1101 4 0.4159590331036185 1101 5 0.4351100144908244 1101 6 0.4652267992735233 1101 7 0.4190243494862216 1101 8 0.3540437126854954 1101 9 0.3806642731868948 1101 10-11 0.4389366266800607 1101 12-13 0.46410451604152314 1101 14-15 0.5500755512611235 1101 16-17 0.5405152361288614 1101 18-19 0.6054830232248705 1101 20-21 0.6461000441810896 1101 22-23 0.6784939239647017 1101 24-25 0.7126090118062862 1101 26-27 0.6938297766185926 1101 28-29 0.6659608718745389 1101 30-31 0.659347053878669 1101 32-33 0.666307511111583 1101 34-35 0.6851088642081677 1101 36-37 0.625903659949465 1101 38-39 0.6578477751339022 1101 40-41 0.6588440741093962 1101 42-43 0.701643078340382 1101 44-45 0.7008382091722325 1101 46-47 0.651642176630979 1101 48-49 0.7267151789049464 1101 50-51 0.7309035517114637 1101 52-53 0.8137838788311598 1101 54-55 0.8300801797431774 1101 56-57 0.7787233060642365 1101 58-59 0.7182940568302598 1101 60-61 0.7245477230957746 1101 62-63 0.7413421039104193 1101 64-65 0.6920972164028214 1101 66-67 0.674982081154333 1101 68-69 0.6857977963567379 1101 70-71 0.6316080354908351 1101 72-73 0.5793429842202293 1101 74-75 0.5878429207811173 1101 76-77 0.6302014208896445 1101 78-79 0.7163951128189154 1101 80-81 0.6554535383992182 1101 82-83 0.6267560339199392 1101 84-85 0.5794075689934886 1101 86-87 0.6351718095285079 1101 88-89 0.7129249911636677 1101 90-91 0.6960998747468032 1101 92-93 0.7773924263022458 1101 94-95 0.8169590726030194 1101 96-97 0.8164670663294089 1101 98-99 0.874399860989616 1101 100-101 1.0743998297660617 1102 1 0.3598218487283589 1102 2 0.3486422015691133 1102 3 0.33071767369143856 1102 4 0.3594619698430108 1102 5 0.3719666232631553 1102 6 0.35179327264933846 1102 7 0.2951079477124736 1102 8 0.264324743172601 1102 9 0.3157070957151049 1102 10-11 0.34084954392317 1102 12-13 0.3978655778106841 1102 14-15 0.42468166294835186 1102 16-17 0.47312251150295737 1102 18-19 0.5124147496675775 1102 20-21 0.48415184889575613 1102 22-23 0.427471569715145 1102 24-25 0.5038440662914567 1102 26-27 0.5307201504115469 1102 28-29 0.5143989352807026 1102 30-31 0.5199282301596568 1102 32-33 0.4961333025352346 1102 34-35 0.5476077366644674 1102 36-37 0.5793390889385677 1102 38-39 0.5595844903536573 1102 40-41 0.5603978511061953 1102 42-43 0.5467976439361877 1102 44-45 0.5193730922638622 1102 46-47 0.48908155500699735 1102 48-49 0.5560850122000502 1102 50-51 0.6022776342584422 1102 52-53 0.6498876068513866 1102 54-55 0.7099582850510942 1102 56-57 0.585149756726544 1102 58-59 0.5658411626433519 1102 60-61 0.5030595976392505 1102 62-63 0.5582327289272655 1102 64-65 0.5631024136105225 1102 66-67 0.6202510109791959 1102 68-69 0.6300274839544997 1102 70-71 0.6181700702453696 1102 72-73 0.5273552248550111 1102 74-75 0.5611277814465687 1102 76-77 0.5265310827898979 1102 78-79 0.6019134544478 1102 80-81 0.5779803140876147 1102 82-83 0.5533654741012164 1102 84-85 0.5457470472333057 1102 86-87 0.559315880269228 1102 88-89 0.5741482942560943 1102 90-91 0.5912407043655215 1102 92-93 0.6121129227829663 1102 94-95 0.7136988609608608 1102 96-97 0.7148546904388766 1102 98-99 0.752936288563383 1102 100-101 0.9178186868792828 1103 1 0.3271645368692404 1103 2 0.2827637802620586 1103 3 0.3010717677084358 1103 4 0.34558928367577835 1103 5 0.3203736339165246 1103 6 0.29017493619871004 1103 7 0.2752223965759697 1103 8 0.24235868969280006 1103 9 0.2824965366017551 1103 10-11 0.24347737241133416 1103 12-13 0.28741442834259345 1103 14-15 0.35738403332456414 1103 16-17 0.38192315086930506 1103 18-19 0.392607883383441 1103 20-21 0.40759208053566454 1103 22-23 0.3914304968701927 1103 24-25 0.46560546196983665 1103 26-27 0.5003124913609369 1103 28-29 0.4694375348419939 1103 30-31 0.4378170388392135 1103 32-33 0.49156488316551616 1103 34-35 0.4718187565240797 1103 36-37 0.503606614152531 1103 38-39 0.48554047803578726 1103 40-41 0.46311962563572706 1103 42-43 0.449512325552206 1103 44-45 0.48063568876651175 1103 46-47 0.4845295812372399 1103 48-49 0.5064359318506533 1103 50-51 0.4603943661891279 1103 52-53 0.5466156228390915 1103 54-55 0.5761600488208956 1103 56-57 0.5643973455035152 1103 58-59 0.4236319317832482 1103 60-61 0.3978644255183781 1103 62-63 0.41430118943225835 1103 64-65 0.4523220125468157 1103 66-67 0.4791095551359845 1103 68-69 0.4847858497674551 1103 70-71 0.47806544912491233 1103 72-73 0.4577733036020213 1103 74-75 0.4519315663065342 1103 76-77 0.45240039226385065 1103 78-79 0.5649485237079723 1103 80-81 0.5405012653068546 1103 82-83 0.49522557272177536 1103 84-85 0.520341100890441 1103 86-87 0.5109449835923847 1103 88-89 0.49740857453560494 1103 90-91 0.49750158351165297 1103 92-93 0.5514527993944149 1103 94-95 0.5462483107375853 1103 96-97 0.5475070749214161 1103 98-99 0.5435044457647749 1103 100-101 0.7536720034800233 1104 1 0.07357625747192031 1104 2 0.1678872555328894 1104 3 0.1941101054080434 1104 4 0.21628625457227457 1104 5 0.16125885255236483 1104 6 0.18209692237858377 1104 7 0.15439128066855545 1104 8 0.12549945597790924 1104 9 0.16851032960970258 1104 10-11 0.18404794705247696 1104 12-13 0.19195954614946942 1104 14-15 0.1989473339832344 1104 16-17 0.2220346734668155 1104 18-19 0.20544721539179278 1104 20-21 0.19545434206658996 1104 22-23 0.1963710835211856 1104 24-25 0.23077442796463998 1104 26-27 0.23965668082283997 1104 28-29 0.23677710428658116 1104 30-31 0.2588033875706728 1104 32-33 0.3148053891658549 1104 34-35 0.2980548023181626 1104 36-37 0.30489350722952224 1104 38-39 0.2849079971380277 1104 40-41 0.24704676393369596 1104 42-43 0.33601295667786246 1104 44-45 0.3136059718145461 1104 46-47 0.2952127069399424 1104 48-49 0.2943257274021107 1104 50-51 0.31551519383481264 1104 52-53 0.47320767618892745 1104 54-55 0.45527644959459934 1104 56-57 0.46444633596203744 1104 58-59 0.3977274112220286 1104 60-61 0.3308010925461957 1104 62-63 0.3872537130025435 1104 64-65 0.34706212374171486 1104 66-67 0.39777077875456257 1104 68-69 0.33016641446847217 1104 70-71 0.35473144285277414 1104 72-73 0.28381203260164867 1104 74-75 0.3060495783387651 1104 76-77 0.3594793384212238 1104 78-79 0.4767681395396828 1104 80-81 0.4115558830734365 1104 82-83 0.3678725657033972 1104 84-85 0.35453368491155857 1104 86-87 0.3417889388950286 1104 88-89 0.35831614170783155 1104 90-91 0.3711478928967189 1104 92-93 0.4019787281050746 1104 94-95 0.44901050305468715 1104 96-97 0.4171013232725471 1104 98-99 0.39520280169828936 1104 100-101 0.5707412909757537 1105 1 0.18093199311031327 1105 2 0.2245827599196346 1105 3 0.2441181106946999 1105 4 0.2725516874518519 1105 5 0.26026534098669174 1105 6 0.26171271986618905 1105 7 0.2157028895120945 1105 8 0.20062100779635728 1105 9 0.2787904187127239 1105 10-11 0.2917777492583937 1105 12-13 0.2993637434753893 1105 14-15 0.35213912204354614 1105 16-17 0.3576437603701592 1105 18-19 0.3966723312486451 1105 20-21 0.2670959359799525 1105 22-23 0.34260260273418197 1105 24-25 0.37626872281510515 1105 26-27 0.3559749012316331 1105 28-29 0.3796775561879784 1105 30-31 0.4069854212071107 1105 32-33 0.395548425227382 1105 34-35 0.39392862203670376 1105 36-37 0.42928797776632877 1105 38-39 0.42753933838296376 1105 40-41 0.45533589862222357 1105 42-43 0.44671972767980606 1105 44-45 0.4775597261645146 1105 46-47 0.35814102116520274 1105 48-49 0.4332448047725208 1105 50-51 0.4157127370118303 1105 52-53 0.5481672005177884 1105 54-55 0.5767109415303082 1105 56-57 0.5559722367573201 1105 58-59 0.4073635390424428 1105 60-61 0.4186959219885438 1105 62-63 0.46346295871810383 1105 64-65 0.5034300349492753 1105 66-67 0.5165123929179529 1105 68-69 0.5316114069483504 1105 70-71 0.5089852916035227 1105 72-73 0.4621732336284161 1105 74-75 0.534979628193998 1105 76-77 0.5054115867659732 1105 78-79 0.58662215345581 1105 80-81 0.5706046913726901 1105 82-83 0.5147312941724174 1105 84-85 0.5236240401577383 1105 86-87 0.5229322465154382 1105 88-89 0.5835557520082979 1105 90-91 0.5453986395273738 1105 92-93 0.5568792904732263 1105 94-95 0.6409282394018305 1105 96-97 0.6324849163028112 1105 98-99 0.6298637690316333 1105 100-101 0.6849134414810685 1106 1 0.12301934409644844 1106 2 0.1477488051403384 1106 3 0.1434870284649108 1106 4 0.19714898078963472 1106 5 0.14868150565679628 1106 6 0.08867363178949006 1106 7 0.10466069101014597 1106 8 0.13779268025192692 1106 9 0.14246597994870314 1106 10-11 0.10462916304845749 1106 12-13 0.1438292329754347 1106 14-15 0.13007517655425715 1106 16-17 0.05815602448973323 1106 18-19 0.05308811935869784 1106 20-21 0.13249629208766578 1106 22-23 0.12900612802962996 1106 24-25 0.20763787563526392 1106 26-27 0.18446598757589783 1106 28-29 0.14704584356093875 1106 30-31 0.11801029380588801 1106 32-33 0.14508671270374407 1106 34-35 0.16511948979353974 1106 36-37 0.15217859145360535 1106 38-39 0.18459673663446807 1106 40-41 0.21460972649921217 1106 42-43 0.1920492704767156 1106 44-45 0.22058501359164495 1106 46-47 0.13835816220660746 1106 48-49 0.16006122688970947 1106 50-51 0.1862307820121032 1106 52-53 0.26603173714051565 1106 54-55 0.285981365455946 1106 56-57 0.27671892547856203 1106 58-59 0.2285455483765375 1106 60-61 0.1925134266195272 1106 62-63 0.20723083008217458 1106 64-65 0.19567861385465335 1106 66-67 0.22875371009714485 1106 68-69 0.2808200694748493 1106 70-71 0.2410568348091502 1106 72-73 0.17537606244744808 1106 74-75 0.22680005780040702 1106 76-77 0.23499551989752376 1106 78-79 0.3285886506785296 1106 80-81 0.30548853326711267 1106 82-83 0.2568055799761204 1106 84-85 0.2803753580986559 1106 86-87 0.27304568448835553 1106 88-89 0.25607130361145636 1106 90-91 0.18902672340571414 1106 92-93 0.24672927301793024 1106 94-95 0.2628167376836572 1106 96-97 0.1943588751642089 1106 98-99 0.178531473097717 1106 100-101 0.22864274831180253 1107 1 -0.03530304845785537 1107 2 -0.013982835336989297 1107 3 -0.005996434864265154 1107 4 0.07202900133285084 1107 5 -0.007433773505965746 1107 6 0.010520916002271008 1107 7 -0.015533678557311248 1107 8 -0.031114802741790015 1107 9 -0.0327064188265922 1107 10-11 -0.01224303870804988 1107 12-13 -5.012292169439547E-4 1107 14-15 -0.1050898358785517 1107 16-17 -0.09985096838001795 1107 18-19 -0.10173730712606499 1107 20-21 -0.13933881746002186 1107 22-23 -0.06727014240669149 1107 24-25 -0.021876970240207072 1107 26-27 -0.08389904759312117 1107 28-29 -0.062207551840224085 1107 30-31 -0.07531524365352738 1107 32-33 -0.09229838346406183 1107 34-35 -0.06622805714530244 1107 36-37 -0.05869603640221044 1107 38-39 -0.026089728005572965 1107 40-41 -0.05627452422657342 1107 42-43 -0.07210188828869946 1107 44-45 -0.026542809786178623 1107 46-47 -0.06275396441075998 1107 48-49 -0.08081640337685769 1107 50-51 -0.09882233923502781 1107 52-53 -0.01821159188114052 1107 54-55 0.05665687408678366 1107 56-57 -0.043254804919193646 1107 58-59 -0.17194872683862172 1107 60-61 -0.1980612805096058 1107 62-63 -0.18003838481590861 1107 64-65 -0.1674562269789277 1107 66-67 -0.17913916935222574 1107 68-69 -0.13088540906981194 1107 70-71 -0.13292997982675558 1107 72-73 -0.18622082908334292 1107 74-75 -0.20687146695738434 1107 76-77 -0.2000572274997161 1107 78-79 -0.0696914786547076 1107 80-81 -0.10646362068813175 1107 82-83 -0.09099850388508557 1107 84-85 -0.13427398926073408 1107 86-87 -0.044642689132640356 1107 88-89 -0.050540002085380564 1107 90-91 -0.034393594646981995 1107 92-93 -0.005660712338386276 1107 94-95 0.002529789759833534 1107 96-97 -0.022520254658715544 1107 98-99 -0.03859503568721223 1107 100-101 -0.009540873683139495 1108 1 -0.04742292653726565 1108 2 0.04393633729740998 1108 3 0.046698846912725855 1108 4 0.05946695798128587 1108 5 0.006588986864663582 1108 6 0.0163752242305506 1108 7 0.013166315820569707 1108 8 0.043312257723663095 1108 9 -0.002563450589519789 1108 10-11 -0.03434269466176687 1108 12-13 -0.01288335731305068 1108 14-15 -0.08561780346590098 1108 16-17 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-0.9970623864691142 2316 84-85 -1.0207614478982343 2316 86-87 -1.043674887548395 2316 88-89 -1.1457117695874999 2316 90-91 -1.1153516176045812 2316 92-93 -1.1019553662377355 2316 94-95 -1.0681063486633064 2316 96-97 -1.0788926094557958 2316 98-99 -1.0892682265326705 2316 100-101 -1.2871490296990906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 257063.0 3 54853.0 4 50727.0 5 56151.0 6 77351.0 7 137641.0 8 289859.0 9 585075.0 10 784110.0 11 596016.0 12 380153.0 13 281455.0 14 230490.0 15 191378.0 16 165991.0 17 153124.0 18 147762.0 19 148610.0 20 156049.0 21 164894.0 22 179009.0 23 196717.0 24 224627.0 25 254779.0 26 225558.0 27 248501.0 28 278165.0 29 314337.0 30 360248.0 31 422198.0 32 516546.0 33 665441.0 34 911016.0 35 1360327.0 36 2355582.0 37 5531924.0 38 1.0743994E7 39 4735470.0 40 22453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.119317883832174 16.520606767176883 8.829467197356815 46.53060815163413 2 12.294700651549258 16.51123717591667 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27.526696560133 36.572113724602175 17.120171082858025 22-23 19.13387198407282 28.223668657021346 35.661920231927006 16.980539126978826 24-25 18.55561241059424 28.914906918361133 35.135626906810714 17.393853764233913 26-27 19.018186586952986 29.423875511427067 34.04019147052643 17.517746431093514 28-29 18.922901304979884 29.863429604941473 33.3598976280136 17.853771462065048 30-31 19.279809179824657 29.96449393789006 32.8672276035888 17.88846927869648 32-33 19.34107762650425 29.821312500516928 32.821267832021626 18.016342040957188 34-35 19.386129012628583 30.4291832335452 32.507412409192675 17.677275344633543 36-37 18.936254643398172 29.996358387119027 32.880870337480125 18.18651663200268 38-39 19.25782685927532 29.31122591422416 33.200479621607286 18.23046760489323 40-41 19.295646436693755 29.394442131986352 33.25730390024912 18.052607531070773 42-43 19.639005123551037 29.221717132315035 33.19507469600387 17.944203048130056 44-45 19.654629076417624 29.251010798174992 33.19862100470245 17.89573912070493 46-47 18.902527811044948 29.53543718757342 32.97141593985967 18.590619061521966 48-49 19.28893263605617 29.72324537440636 32.233630066078526 18.754191923458944 50-51 19.06646911617092 29.837635636771516 32.453110522822456 18.642784724235106 52-53 19.28006401942723 30.175984965805426 32.09362258601664 18.450328428750712 54-55 19.179334398632214 30.757257043698267 31.198600625080942 18.864807932588576 56-57 19.255384740839954 30.63105054598423 31.803066947449437 18.31049776572638 58-59 19.19444826575384 30.610120163526016 31.479367055985747 18.716064514734395 60-61 19.32422360977655 31.44211293851943 30.346838098277217 18.886825353426804 62-63 19.35081508503334 31.229074007588338 30.98130804859868 18.43880285877964 64-65 19.726851888029167 30.95547153618176 30.719829737743687 18.59784683804539 66-67 19.29938777331372 31.347554549708377 30.345713345313186 19.007344331664715 68-69 19.513410607445252 30.890854771003724 30.830912795324455 18.764821826226573 70-71 19.508716628899087 30.882186147468914 30.63235178313022 18.97674544050178 72-73 19.098480991688877 31.356671150011927 30.77746748179181 18.767380376507386 74-75 19.177285790371865 30.625041710687444 31.221275409503917 18.97639708943677 76-77 19.256260007401888 31.0231624081784 30.78947275097323 18.931104833446486 78-79 19.428730643782956 31.080555887375105 30.627448417427267 18.863265051414675 80-81 19.287989884470853 30.992903701218218 30.814503618344446 18.904602795966486 82-83 19.38953165344361 31.012548276728992 30.680812626138827 18.917107443688575 84-85 19.291715742916942 31.08325638610407 30.556810455120203 19.068217415858786 86-87 19.58420588084977 31.175547853420127 30.285280187639778 18.954966078090326 88-89 19.302415050434206 31.154289655547995 30.43859306642766 19.10470222759014 90-91 19.407756539600975 31.104748529410504 30.611510393332896 18.875984537655626 92-93 19.53986996512223 30.91593926068117 30.477739981744627 19.066450792451974 94-95 19.390424074379204 31.116697349693283 30.31346423756181 19.1794143383657 96-97 19.32698458621639 31.12185617237206 30.22044336999969 19.33071587141186 98-99 19.411508355610376 31.13170074412918 30.422797487610666 19.033993412649775 100-101 19.653079627546937 31.597959576844385 29.61331486427403 19.135645931334643 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12105.0 1 23445.0 2 32278.5 3 34337.0 4 43913.0 5 54548.5 6 65449.5 7 76655.5 8 88309.0 9 99868.0 10 110781.0 11 120524.0 12 130097.5 13 139140.5 14 147176.5 15 154761.5 16 163088.5 17 171574.0 18 184017.0 19 202599.5 20 227686.5 21 258338.5 22 299000.0 23 349497.5 24 417725.5 25 499131.0 26 589705.0 27 695578.5 28 815112.5 29 900263.5 30 991445.5 31 1103275.0 32 1212001.0 33 1275798.0 34 1255261.5 35 1278963.5 36 1319156.5 37 1357752.0 38 1427807.0 39 1349828.5 40 1255330.5 41 1205898.5 42 1092932.0 43 1029459.0 44 989612.0 45 942313.0 46 898523.5 47 856109.5 48 808881.5 49 777096.5 50 720688.0 51 636810.0 52 572539.5 53 509959.0 54 446501.0 55 380759.5 56 323700.5 57 266020.5 58 213127.0 59 177593.5 60 140691.5 61 103008.0 62 77287.0 63 59546.0 64 40667.0 65 28844.5 66 22672.5 67 17055.0 68 12139.5 69 9077.5 70 10950.0 71 17574.0 72 17479.0 73 28076.5 74 31112.0 75 15774.0 76 9819.5 77 13890.5 78 13367.5 79 5186.0 80 2483.5 81 952.5 82 496.5 83 306.0 84 199.0 85 165.0 86 159.5 87 117.0 88 101.0 89 111.0 90 132.0 91 139.0 92 115.5 93 81.5 94 39.5 95 12.0 96 4.0 97 4.0 98 6.0 99 3.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4451897633955122 2 0.34276532460110165 3 0.31981117520253 4 0.3909490125913769 5 0.3571432302934172 6 0.2838344858682659 7 0.2589532211326539 8 0.2640467262779938 9 0.2559493591238637 10-11 0.25831762134528674 12-13 0.23509646199037812 14-15 0.2239255780562395 16-17 0.2086842434290301 18-19 0.24331717613520734 20-21 0.2600618929078789 22-23 0.21817035258432554 24-25 0.2455519333784619 26-27 0.24465222591689184 28-29 0.21922533214006973 30-31 0.23167176907214387 32-33 0.16774755392759455 34-35 0.22291848615570792 36-37 0.21448010085082142 38-39 0.20375181494213254 40-41 0.22941089128968245 42-43 0.20831565359799978 44-45 0.48905630671131844 46-47 0.13135583824815464 48-49 0.15650411294010352 50-51 0.15665212932894246 52-53 0.3731290002880225 54-55 0.26124747515965746 56-57 0.4170855143499857 58-59 0.37899741476316623 60-61 0.12798628869046824 62-63 0.14039789823693324 64-65 0.09163375381983863 66-67 0.12119059507348057 68-69 0.14231920900970535 70-71 0.13944740083801657 72-73 0.17254937971845774 74-75 0.1726378993235477 76-77 0.15558554064466187 78-79 0.21624033496515116 80-81 0.14950235729159495 82-83 0.1374912626796353 84-85 0.15620227559815744 86-87 0.13935162552759134 88-89 0.12950998681086906 90-91 0.20028213665081981 92-93 0.1956384852362649 94-95 0.2163041851721013 96-97 0.16632543568188712 98-99 0.18389730286277628 100-101 0.17338088354987646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.4455644E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.752502373745767 #Duplication Level Percentage of deduplicated Percentage of total 1 76.11673167947427 18.840795815779565 2 8.948050616004338 4.429732882260893 3 3.669991770165925 2.725244400079785 4 2.1454037287234713 2.1241644355148295 5 1.361561674788317 1.685102929359954 6 1.0143813256091645 1.5065085702014531 7 0.7831438444231292 1.3569338907647521 8 0.6058676332380144 1.1997392023919744 9 0.5057542028519146 1.1266813895961523 >10 3.8912939868092327 19.51673458955609 >50 0.5168467402119831 8.947698291229717 >100 0.3869849806490239 18.71085407968749 >500 0.03445648278125095 5.792441850227234 >1k 0.018031297272517177 7.900514401997258 >5k 0.0010512070299196 1.7423638203933463 >10k+ 4.4882996751846154E-4 2.3944894509592984 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 100390 0.29136010344197893 Illumina Single End PCR Primer 1 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84895 0.2463892417741488 No Hit CAAGCAGAAGACGGCATACGAGATTGGAAGAGCGTCGTGTAGGGAAAGAG 51970 0.1508316025090113 Illumina Single End PCR Primer 1 (96% over 30bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 39749 0.11536281254821416 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.34482014035204217 0.0 0.0 0.0 0.0 2 0.347722422486139 0.0 0.0 0.0 0.0 3 0.34802716211021917 0.0 0.0 0.0 0.0 4 0.38606737404182606 0.0 0.0 0.0 0.0 5 0.3868625993465686 0.0 0.0 0.0 0.0 6 0.38918442505384604 0.0 0.0 0.0 0.0 7 0.3916513648678283 2.9022821340968116E-6 0.0 0.0 0.0 8 0.40666487034751114 2.9022821340968116E-6 0.0 0.0 0.0 9 0.4074136591381081 5.804564268193623E-6 0.0 5.804564268193623E-6 0.0 10-11 0.4134184808735544 8.706846402290435E-6 0.0 8.706846402290435E-6 0.0 12-13 0.4181738701502721 8.706846402290435E-6 0.0 8.706846402290435E-6 0.0 14-15 0.4212691540462863 2.031597493867768E-5 0.0 8.706846402290435E-6 0.0 16-17 0.42538749239456963 2.031597493867768E-5 0.0 8.706846402290435E-6 0.0 18-19 0.4299687447432415 2.031597493867768E-5 0.0 1.3060269603435653E-5 0.0 20-21 0.4382547602360879 2.1767116005726086E-5 0.0 1.741369280458087E-5 0.0 22-23 0.4453000501166079 2.3218257072774492E-5 0.0 1.741369280458087E-5 0.0 24-25 0.44986679105460925 2.3218257072774492E-5 0.0 2.031597493867768E-5 0.0 26-27 0.4543798397731298 2.3218257072774492E-5 0.0 2.46693981398229E-5 0.0 28-29 0.45866941276732487 2.3218257072774492E-5 0.0 2.757168027391971E-5 0.0 30-31 0.46259474935369077 2.3218257072774492E-5 0.0 2.9022821340968116E-5 0.0 32-33 0.4668770666425507 2.3218257072774492E-5 0.0 2.9022821340968116E-5 0.0 34-35 0.47219549865328303 2.3218257072774492E-5 0.0 2.9022821340968116E-5 0.0 36-37 0.47662728347204886 2.3218257072774492E-5 0.0 3.1925103475064925E-5 0.0 38-39 0.48150166631626445 2.3218257072774492E-5 0.0 3.337624454211333E-5 0.0 40-41 0.4867576992611138 2.9022821340968116E-5 0.0 3.482738560916174E-5 0.0 42-43 0.4917583313781626 2.9022821340968116E-5 0.0 3.7729667743258545E-5 0.0 44-45 0.49731184824175684 2.9022821340968116E-5 2.9022821340968116E-6 4.063194987735536E-5 0.0 46-47 0.5058793851016106 2.9022821340968116E-5 2.9022821340968116E-6 4.063194987735536E-5 0.0 48-49 0.5118900114013252 2.9022821340968116E-5 2.9022821340968116E-6 4.063194987735536E-5 0.0 50-51 0.5175668752556184 3.047396240801652E-5 2.9022821340968116E-6 4.063194987735536E-5 0.0 52-53 0.5239881744773077 3.1925103475064925E-5 2.9022821340968116E-6 4.2083090944403765E-5 0.0 54-55 0.5309710652919446 3.1925103475064925E-5 4.353423201145217E-6 4.353423201145217E-5 0.0 56-57 0.5387056471793126 3.1925103475064925E-5 5.804564268193623E-6 4.498537307850058E-5 0.0 58-59 0.5467130435872858 3.1925103475064925E-5 8.706846402290435E-6 4.6436514145548985E-5 0.0 60-61 0.5549700362587912 3.1925103475064925E-5 8.706846402290435E-6 4.6436514145548985E-5 0.0 62-63 0.5629092290366129 3.1925103475064925E-5 8.706846402290435E-6 4.6436514145548985E-5 0.0 64-65 0.5715478718087521 3.337624454211333E-5 8.706846402290435E-6 4.93387962796458E-5 0.0 66-67 0.5812327872902332 3.482738560916174E-5 8.706846402290435E-6 5.2241078413742605E-5 0.0 68-69 0.5914531738254551 3.482738560916174E-5 8.706846402290435E-6 5.2241078413742605E-5 0.0 70-71 0.6025297335902355 3.482738560916174E-5 8.706846402290435E-6 5.2241078413742605E-5 0.0 72-73 0.6146090318323465 3.482738560916174E-5 8.706846402290435E-6 5.804564268193623E-5 0.0 74-75 0.6279972593169352 3.918080881030695E-5 8.706846402290435E-6 5.804564268193623E-5 0.0 76-77 0.6428685529720473 4.353423201145217E-5 8.706846402290435E-6 5.804564268193623E-5 0.0 78-79 0.6595174944342936 4.353423201145217E-5 8.706846402290435E-6 5.804564268193623E-5 0.0 80-81 0.6779760088071493 4.353423201145217E-5 8.706846402290435E-6 6.0947924816033044E-5 0.0 82-83 0.6989130721225236 4.353423201145217E-5 8.706846402290435E-6 6.675248908422666E-5 0.0 84-85 0.7234359050145747 4.353423201145217E-5 1.1609128536387246E-5 6.675248908422666E-5 0.0 86-87 0.7520567602799704 4.353423201145217E-5 1.1609128536387246E-5 6.675248908422666E-5 0.0 88-89 0.784994760219835 4.353423201145217E-5 1.1609128536387246E-5 6.675248908422666E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGGC 16205 0.0 67.20167 9 AAGACGG 17340 0.0 63.328407 8 GTTTTTT 598640 0.0 50.08044 1 CGCGTGT 18990 0.0 49.893 1 GAAGACG 27640 0.0 40.021175 7 CGGCATA 14360 0.0 37.957363 12-13 GCGTGTT 25275 0.0 37.6283 2 ACGGCAT 15185 0.0 35.71513 10-11 CAAGCAG 32915 0.0 34.71296 1 GACGGCA 16150 0.0 33.860176 10-11 ATACGAG 16590 0.0 32.649597 16-17 TACGAGA 16495 0.0 32.305447 16-17 AGATCGG 39455 0.0 29.200218 1 GCATACG 19005 0.0 29.056927 14-15 GCAGAAG 40835 0.0 27.735071 4 GGCATAC 20445 0.0 26.961834 12-13 AGAAGAC 42445 0.0 26.621569 6 CGTGTTT 37060 0.0 26.252832 3 GATCGGA 44710 0.0 25.832136 2 ACGAGAT 21350 0.0 25.827637 18-19 >>END_MODULE