FastQCFastQC Report
Fri 27 May 2016
SRR1233092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34455644
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1030710.29914112184349245TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC797590.23148312073342758TruSeq Adapter, Index 4 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT749690.21758118931110387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT185500.054.8258361
GTTTTTT5758850.050.5951461
GCGTGTT251500.041.1226582
AGATCGG374150.031.598271
CGTGTTT377400.027.6551383
GATCGGA445500.026.8268682
ATCGGAA457650.026.0622253
TAAACGC51700.024.328612
TCGGAAG508500.023.448274
CGGAAGA528450.022.5640375
CTCGTAT456500.021.93376442-43
ATCTCGT452000.021.86725440-41
GCCGTCT560050.021.308876
CGTCTGA501750.021.2998816-17
TATGCCG563950.021.1749553
ATGCCGT564100.021.1371124
CACGTCT508300.020.85146314-15
TGCCGTC573150.020.812555
CCCCGCG89550.020.7321158
CGGAGAT63100.020.6925031
Produced by FastQC (version 0.11.5)