##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34455644 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.831932730672513 34.0 31.0 34.0 30.0 34.0 2 32.08264930413142 34.0 31.0 34.0 30.0 34.0 3 32.19650966326446 34.0 31.0 34.0 30.0 34.0 4 35.42573901680665 37.0 37.0 37.0 35.0 37.0 5 35.299530143740746 37.0 37.0 37.0 33.0 37.0 6 35.25169896693848 37.0 37.0 37.0 33.0 37.0 7 35.26985860429716 37.0 36.0 37.0 33.0 37.0 8 35.19744268892492 37.0 36.0 37.0 33.0 37.0 9 36.94470360211523 39.0 39.0 39.0 34.0 39.0 10-11 36.92926174881538 39.0 38.0 39.0 34.0 39.0 12-13 36.90335416456009 39.0 38.0 39.0 34.0 39.0 14-15 38.38649781150514 41.0 39.0 41.0 34.0 41.0 16-17 38.216805220648325 41.0 39.0 41.0 34.0 41.0 18-19 37.737231250125525 41.0 38.0 41.0 33.0 41.0 20-21 37.31661207667457 41.0 38.0 41.0 32.5 41.0 22-23 36.99653635845553 41.0 38.0 41.0 31.5 41.0 24-25 36.53008450516844 41.0 38.0 41.0 28.5 41.0 26-27 35.882710899265156 41.0 38.0 41.0 22.0 41.0 28-29 35.540235657763354 40.0 38.0 41.0 16.5 41.0 30-31 35.129750673068244 40.0 37.0 41.0 8.5 41.0 32-33 35.00764059148045 41.0 37.5 41.0 6.0 41.0 34-35 34.94957797044803 41.0 38.0 41.0 2.0 41.0 36-37 34.8753803730965 41.0 37.5 41.0 2.0 41.0 38-39 34.77809149351555 41.0 37.0 41.0 2.0 41.0 40-41 34.670106282152204 40.0 37.0 41.0 2.0 41.0 42-43 34.547449686327155 40.0 37.0 41.0 2.0 41.0 44-45 34.46084359938244 40.0 37.0 41.0 2.0 41.0 46-47 34.33750802916352 40.0 36.5 41.0 2.0 41.0 48-49 34.23580636020038 40.0 36.0 41.0 2.0 41.0 50-51 34.09048994991938 40.0 36.0 41.0 2.0 41.0 52-53 33.91599054134643 40.0 35.0 41.0 2.0 41.0 54-55 33.863588342740016 40.0 35.0 41.0 2.0 41.0 56-57 33.79734928187672 40.0 35.0 41.0 2.0 41.0 58-59 33.6256093921797 40.0 35.0 41.0 2.0 41.0 60-61 33.41916735034759 40.0 35.0 41.0 2.0 41.0 62-63 33.14402726009126 39.0 35.0 41.0 2.0 41.0 64-65 32.80907828917666 39.0 34.5 41.0 2.0 41.0 66-67 32.600477036505254 39.0 34.0 41.0 2.0 41.0 68-69 32.19905582086929 37.5 34.0 40.5 2.0 41.0 70-71 31.884527669835457 37.0 34.0 40.0 2.0 41.0 72-73 31.537129882117426 37.0 33.5 39.5 2.0 41.0 74-75 31.152805386542767 36.0 33.0 39.0 2.0 41.0 76-77 30.081296927725397 35.0 31.5 37.5 2.0 39.0 78-79 30.403452638992903 35.0 32.5 37.5 2.0 39.5 80-81 30.217598153150178 35.0 33.0 37.0 2.0 39.0 82-83 29.91319716734942 35.0 33.0 37.0 2.0 39.0 84-85 29.628093237787112 35.0 33.0 36.0 2.0 38.0 86-87 29.350851155183747 35.0 32.0 36.0 2.0 37.0 88-89 29.111431366657953 35.0 32.0 36.0 2.0 37.0 90-91 28.88647243104787 35.0 32.0 35.0 2.0 36.5 92-93 28.68059013785956 35.0 32.0 35.0 2.0 36.0 94-95 28.505142901987263 35.0 31.5 35.0 2.0 36.0 96-97 28.334924722347374 35.0 31.0 35.0 2.0 36.0 98-99 28.130008366698938 35.0 31.0 35.0 2.0 36.0 100-101 27.247062963037347 34.5 28.0 35.0 2.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2222757641203792 1101 2 0.24729437398333687 1101 3 0.27558803056059844 1101 4 0.20683007651329177 1101 5 0.2819038356580279 1101 6 0.290481777556991 1101 7 0.29231917645112304 1101 8 0.11423798879187785 1101 9 0.21617218483827116 1101 10-11 0.2349100179384962 1101 12-13 0.30366529555470123 1101 14-15 0.43729017676927384 1101 16-17 0.4867312417332812 1101 18-19 0.5234927519174377 1101 20-21 0.5170897136222194 1101 22-23 0.5264040824625695 1101 24-25 0.5203561033871935 1101 26-27 0.6227759414129892 1101 28-29 0.4961148033565763 1101 30-31 0.582561683184295 1101 32-33 0.5365063551903688 1101 34-35 0.5317838629853568 1101 36-37 0.4692599972779732 1101 38-39 0.5404437187743838 1101 40-41 0.5447511875133202 1101 42-43 0.5369951758158749 1101 44-45 0.5401327122049082 1101 46-47 0.5644354744164914 1101 48-49 0.42394687951671983 1101 50-51 0.4856168714130433 1101 52-53 0.5655366843622573 1101 54-55 0.5541479525136452 1101 56-57 0.531749469061765 1101 58-59 0.5894405080616139 1101 60-61 0.49623633422783797 1101 62-63 0.5121202172292172 1101 64-65 0.554173523802028 1101 66-67 0.5976244065025682 1101 68-69 0.6201164987090948 1101 70-71 0.40657467544768267 1101 72-73 0.4312963738511968 1101 74-75 0.3389891672198928 1101 76-77 0.34833826858821837 1101 78-79 0.43387921675076413 1101 80-81 0.39228424247832905 1101 82-83 0.4324107587167738 1101 84-85 0.41461924631056135 1101 86-87 0.42209191718258765 1101 88-89 0.44172937803018897 1101 90-91 0.46348144108747746 1101 92-93 0.5093604372017921 1101 94-95 0.5141093524082798 1101 96-97 0.49237444260993257 1101 98-99 0.4873065905334677 1101 100-101 0.5810199427873499 1102 1 0.21866581145079422 1102 2 0.21660190252536182 1102 3 0.21575911426162975 1102 4 0.18934380014596286 1102 5 0.24279640161347515 1102 6 0.2065256934577775 1102 7 0.23064785824823986 1102 8 0.13111104559300202 1102 9 0.18592386133860117 1102 10-11 0.2177492765933664 1102 12-13 0.29370035195360344 1102 14-15 0.3593922716653779 1102 16-17 0.3652120340342009 1102 18-19 0.36545789054317623 1102 20-21 0.38631732914080885 1102 22-23 0.34742627871884935 1102 24-25 0.35592155601823094 1102 26-27 0.4543142717852149 1102 28-29 0.3899679896714474 1102 30-31 0.46847194156703864 1102 32-33 0.35978675805128546 1102 34-35 0.34599181641689114 1102 36-37 0.3700143169659569 1102 38-39 0.40678247821768565 1102 40-41 0.4297425469345626 1102 42-43 0.47618240318035987 1102 44-45 0.4666930279096988 1102 46-47 0.47093448132437743 1102 48-49 0.4301872447514441 1102 50-51 0.37790001294817444 1102 52-53 0.34535333477361263 1102 54-55 0.4619068905968575 1102 56-57 0.4578332017626252 1102 58-59 0.4822375900692464 1102 60-61 0.4732099514105599 1102 62-63 0.505095384480235 1102 64-65 0.4992126919587392 1102 66-67 0.4899139044088727 1102 68-69 0.5617652924340604 1102 70-71 0.4557542078719088 1102 72-73 0.34078083890501176 1102 74-75 0.32786870689423253 1102 76-77 0.07412448168265584 1102 78-79 0.3715215783153596 1102 80-81 0.3606321297051558 1102 82-83 0.30472780561720825 1102 84-85 0.31628761927010984 1102 86-87 0.3455541182056763 1102 88-89 0.36148020931635116 1102 90-91 0.36272636673028913 1102 92-93 0.3706091887987917 1102 94-95 0.42712581610211586 1102 96-97 0.42340353183135804 1102 98-99 0.4166093969640876 1102 100-101 0.4776955171212123 1103 1 0.2333435472417129 1103 2 0.21016620788074647 1103 3 0.18821267758639237 1103 4 0.15527459823058365 1103 5 0.20732806301866447 1103 6 0.23370457507096631 1103 7 0.2126876667455022 1103 8 0.18159587948230183 1103 9 0.2535319970008558 1103 10-11 0.27399579160471177 1103 12-13 0.2617180117355531 1103 14-15 0.32361727526467376 1103 16-17 0.35292229517033036 1103 18-19 0.3494541463105847 1103 20-21 0.37973765749176636 1103 22-23 0.43144253662844534 1103 24-25 0.450064661711572 1103 26-27 0.5977103026131871 1103 28-29 0.5015620791839694 1103 30-31 0.4998469063504629 1103 32-33 0.49716073432431074 1103 34-35 0.5060860414472401 1103 36-37 0.4974828701099838 1103 38-39 0.4777699482952116 1103 40-41 0.4715611516170952 1103 42-43 0.48574467805569554 1103 44-45 0.4644311937589478 1103 46-47 0.4164399458926411 1103 48-49 0.411457971677585 1103 50-51 0.4773960235436192 1103 52-53 0.4937703604443655 1103 54-55 0.5111655345013517 1103 56-57 0.5172106172052011 1103 58-59 0.5615649893302503 1103 60-61 0.5735720239230844 1103 62-63 0.6067860104367284 1103 64-65 0.6293163089615419 1103 66-67 0.5669681288943451 1103 68-69 0.6436362780859213 1103 70-71 0.5385624493192758 1103 72-73 0.5453221201982501 1103 74-75 0.5341616667791946 1103 76-77 0.5217531431745037 1103 78-79 0.589685627338433 1103 80-81 0.5321359372547221 1103 82-83 0.5100401887248793 1103 84-85 0.515555351669331 1103 86-87 0.5330223225189208 1103 88-89 0.5422040598460107 1103 90-91 0.5331536075801075 1103 92-93 0.5538143740176693 1103 94-95 0.5194094131111626 1103 96-97 0.4875312058757082 1103 98-99 0.5467756076285895 1103 100-101 0.5594891630686689 1104 1 0.12132131224261045 1104 2 0.1570362708845181 1104 3 0.13691213586492523 1104 4 0.11984909303048852 1104 5 0.11934662101752025 1104 6 0.16291054726973186 1104 7 0.127990776508895 1104 8 0.1084237402465078 1104 9 0.13234444755889996 1104 10-11 0.1556562802470154 1104 12-13 0.20736068231214944 1104 14-15 0.23284489855964097 1104 16-17 0.1577576487742931 1104 18-19 0.24907039422954824 1104 20-21 0.2731840085998485 1104 22-23 0.2847512027812016 1104 24-25 0.2981942789545542 1104 26-27 0.38609875974718477 1104 28-29 0.32874655030668976 1104 30-31 0.4045290869020519 1104 32-33 0.3559098603897013 1104 34-35 0.3581240574831739 1104 36-37 0.3480683813257386 1104 38-39 0.2691389234663646 1104 40-41 0.2566110235245418 1104 42-43 0.3107268223186139 1104 44-45 0.23880728803076323 1104 46-47 0.32713666730305846 1104 48-49 0.3217728519759362 1104 50-51 0.36670796071095424 1104 52-53 0.3615636917068059 1104 54-55 0.40878422353271304 1104 56-57 0.2974392603080034 1104 58-59 0.4043185907900053 1104 60-61 0.4361633633291646 1104 62-63 0.38677887304216796 1104 64-65 0.3982343175169305 1104 66-67 0.4230593903122255 1104 68-69 0.5215264404125577 1104 70-71 0.43594065082915634 1104 72-73 0.4304993287030072 1104 74-75 0.39208054073821685 1104 76-77 0.1315573600831108 1104 78-79 0.4027522347622998 1104 80-81 0.3951856241692191 1104 82-83 0.39044010111018324 1104 84-85 0.3754627293610575 1104 86-87 0.4132976257582186 1104 88-89 0.42292644203320506 1104 90-91 0.4142321706961454 1104 92-93 0.46496031462258003 1104 94-95 0.44141147884321796 1104 96-97 0.4284568371433046 1104 98-99 0.4318638133471744 1104 100-101 0.47047102385278095 1105 1 0.13278882439745843 1105 2 0.169050763286684 1105 3 0.14694075894243497 1105 4 0.13205792146667505 1105 5 0.13965446784303026 1105 6 0.19220168698040396 1105 7 0.1833687658385088 1105 8 0.09917267809548491 1105 9 0.14147207015894736 1105 10-11 0.16547132413144539 1105 12-13 0.22881276016474317 1105 14-15 0.26980830927195854 1105 16-17 0.28555059413523054 1105 18-19 0.2582080403746545 1105 20-21 0.25670314089168755 1105 22-23 0.2698655887434924 1105 24-25 0.2392389762694762 1105 26-27 0.393449073668414 1105 28-29 0.28400827027729036 1105 30-31 0.3181588276737912 1105 32-33 0.3196749263966865 1105 34-35 0.29064757775123695 1105 36-37 0.2972749835593689 1105 38-39 0.29452966287175997 1105 40-41 0.3194589679778801 1105 42-43 0.3207783152779271 1105 44-45 0.3201498669788805 1105 46-47 0.2935055681889125 1105 48-49 0.3080019899872468 1105 50-51 0.29834945869456675 1105 52-53 0.3008076842927423 1105 54-55 0.2671315607230085 1105 56-57 0.2829649887428687 1105 58-59 0.3142569917246121 1105 60-61 0.292311222644706 1105 62-63 0.27680324196936823 1105 64-65 0.30189551701472794 1105 66-67 0.15885739996316062 1105 68-69 0.3321974132973651 1105 70-71 0.2927264452563527 1105 72-73 0.28563297251793074 1105 74-75 0.26899004564416273 1105 76-77 0.21685994088912963 1105 78-79 0.21073577522812315 1105 80-81 0.16888893709930386 1105 82-83 0.26239341688714646 1105 84-85 0.3121052107902358 1105 86-87 0.29687587588908926 1105 88-89 0.32010412112130027 1105 90-91 0.28928874696882545 1105 92-93 0.3454442581529449 1105 94-95 0.3406460803098703 1105 96-97 0.3112991704287644 1105 98-99 0.33965891146690197 1105 100-101 0.3388870978722025 1106 1 0.060321219117543734 1106 2 0.10544375571485887 1106 3 0.05233103374879278 1106 4 0.031470619271239286 1106 5 0.015539072592574144 1106 6 0.070661866580636 1106 7 0.04254943188375648 1106 8 0.07763125758528844 1106 9 0.1163390046787427 1106 10-11 0.07338828947896303 1106 12-13 0.06209508148953091 1106 14-15 0.046604333284967936 1106 16-17 0.05810028381346655 1106 18-19 -0.006335592329499207 1106 20-21 0.024653242676002662 1106 22-23 0.050400561766771546 1106 24-25 0.054069675996878175 1106 26-27 0.07154929697578183 1106 28-29 0.04138513586094916 1106 30-31 0.08403240762947206 1106 32-33 0.06788440916424321 1106 34-35 0.039476657303517015 1106 36-37 0.0016069868481736194 1106 38-39 0.03805644671048469 1106 40-41 0.03060704660151714 1106 42-43 -0.0047781005465239446 1106 44-45 -5.447831890137422E-4 1106 46-47 0.07400207193661856 1106 48-49 0.034268539333041304 1106 50-51 0.023223803223316963 1106 52-53 0.010427952044629762 1106 54-55 -0.03739357991353387 1106 56-57 0.011933208358371417 1106 58-59 -0.020978635768045706 1106 60-61 0.001258363420816977 1106 62-63 0.07517513346717664 1106 64-65 0.07552724420023793 1106 66-67 0.13376643236406238 1106 68-69 0.16172942763607523 1106 70-71 0.06923946419195559 1106 72-73 0.024611332351447857 1106 74-75 0.02396668502309396 1106 76-77 0.002944805515110005 1106 78-79 0.03187062232612092 1106 80-81 0.004087069919108899 1106 82-83 0.010403228614496385 1106 84-85 0.07717732318586812 1106 86-87 0.06962145245099904 1106 88-89 0.08859070039609662 1106 90-91 0.045507355057100796 1106 92-93 0.09807496550758188 1106 94-95 0.11942139438990296 1106 96-97 0.07954153050992474 1106 98-99 0.11613228417029475 1106 100-101 0.10459147826423987 1107 1 -0.03170875923687788 1107 2 -0.024830349754637382 1107 3 -0.01801251623780331 1107 4 0.00724315063715153 1107 5 0.005466315759399265 1107 6 0.01696535528471088 1107 7 0.019987327932938115 1107 8 0.0020480779129243842 1107 9 -0.07298291594577222 1107 10-11 -0.06976734262969586 1107 12-13 -0.04704289579645149 1107 14-15 -0.06364893276957417 1107 16-17 -0.08688416974619884 1107 18-19 -0.07007092015007998 1107 20-21 -0.07475749964265788 1107 22-23 -0.12285652661010005 1107 24-25 -0.11058339826994512 1107 26-27 -0.039167283898642324 1107 28-29 -0.13491712618407803 1107 30-31 -0.10997923509430052 1107 32-33 -0.1278070966435081 1107 34-35 -0.20822187277777715 1107 36-37 -0.21931345524830448 1107 38-39 -0.2104830394973618 1107 40-41 -0.17351351731297626 1107 42-43 -0.18823906435758886 1107 44-45 -0.14985153969996645 1107 46-47 -0.18529513939740383 1107 48-49 -0.17494877486673488 1107 50-51 -0.16811087874350505 1107 52-53 -0.19746309313126886 1107 54-55 -0.28282549207843033 1107 56-57 -0.22690144565287085 1107 58-59 -0.20796999139699324 1107 60-61 -0.17258620249101142 1107 62-63 -0.1456560165636489 1107 64-65 -0.13089914802315405 1107 66-67 -0.13876768828243513 1107 68-69 -0.12622734256802914 1107 70-71 -0.20945542344234624 1107 72-73 -0.2086824646172758 1107 74-75 -0.19170035672921415 1107 76-77 -0.23488599249607844 1107 78-79 -0.13436463325948722 1107 80-81 -0.15470491347648263 1107 82-83 -0.13097998459166504 1107 84-85 -0.16226279982330638 1107 86-87 -0.15571142327958754 1107 88-89 -0.1786078923728418 1107 90-91 -0.19479012902304405 1107 92-93 -0.1262927717262734 1107 94-95 -0.10798689454212607 1107 96-97 -0.11440129722408088 1107 98-99 -0.08063614568635202 1107 100-101 -0.06841656853159606 1108 1 8.021093275267788E-4 1108 2 0.029586405008409145 1108 3 0.0344334699202804 1108 4 0.04742108019728164 1108 5 0.05252960390406969 1108 6 0.0834968454749756 1108 7 0.06625735784603393 1108 8 0.052931973828769685 1108 9 0.08146860459653027 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-0.43803404227377385 2316 74-75 -0.5046262353986499 2316 76-77 -0.4945863203677625 2316 78-79 -0.4950717659951245 2316 80-81 -0.5384215588706489 2316 82-83 -0.5797004153085972 2316 84-85 -0.5671907335015121 2316 86-87 -0.5636008790341016 2316 88-89 -0.5921404709906888 2316 90-91 -0.6059402842630952 2316 92-93 -0.6693494711900136 2316 94-95 -0.6940773821453909 2316 96-97 -0.757254295818246 2316 98-99 -0.7408681369597154 2316 100-101 -0.9473039458574277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 636765.0 3 147598.0 4 86680.0 5 94570.0 6 310239.0 7 302812.0 8 215732.0 9 396428.0 10 626410.0 11 576912.0 12 363062.0 13 246602.0 14 205971.0 15 180094.0 16 156369.0 17 135550.0 18 121363.0 19 114937.0 20 110454.0 21 112484.0 22 124165.0 23 139438.0 24 150706.0 25 166898.0 26 189308.0 27 215157.0 28 253245.0 29 237852.0 30 265893.0 31 310019.0 32 382831.0 33 495174.0 34 693726.0 35 1112431.0 36 2018925.0 37 5323239.0 38 1.1773842E7 39 5435568.0 40 26195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.95872490614379 16.87237457846214 8.914965423765638 46.25393509162844 2 12.8785548760672 16.22382850374899 60.52319423076187 10.374422389421948 3 15.982211796708834 21.90844263424593 41.91329583043057 20.196049738614665 4 18.346417025359997 29.276644682239912 35.949780476024564 16.42715781637553 5 19.696808207482235 30.687043242150473 35.69368312591541 13.922465424451874 6 15.440059562505033 33.67105665034723 37.01230510904826 13.876578678099474 7 13.01626289859745 18.297020938241424 53.946036198591564 14.740679964569567 8 13.064010766027181 20.7240267806583 47.42501015412026 18.786952299194258 9 15.232428645813965 20.882971863335346 46.247372342915746 17.637227147934944 10-11 18.421576851673972 30.37979307854951 35.20392278376869 15.994707286007822 12-13 17.008395627906218 24.484519416388178 41.60612938958058 16.90095556612502 14-15 17.062847157993787 26.56646761925021 39.96707826876806 16.40360695398794 16-17 18.268077479180764 26.717592439974787 38.01706691085429 16.997263169990156 18-19 18.44955160648384 27.088586638250757 37.66791160794745 16.793950147317954 20-21 18.930058808702114 27.273336803462765 36.33003865928196 17.46656572855316 22-23 18.686480235772326 28.909395972910428 35.295616144466244 17.108507646851 24-25 18.535984135158834 28.94426020749798 34.55625350488119 17.963502152461995 26-27 18.889413764278245 29.08399727808041 33.837942247404285 18.188646710237062 28-29 18.97015242637118 29.85610102911308 32.958653744133045 18.215092800382692 30-31 19.241726373623656 29.88154756476054 32.575528082379385 18.301197979236424 32-33 19.277028285318178 30.016678666399855 32.07719797473274 18.629095073549223 34-35 19.243044340697686 30.20854662396281 32.321428514272796 18.22698052106671 36-37 19.175915883550495 30.288033656727887 32.07141406169581 18.464636398025803 38-39 19.456969207600025 29.425374397200827 32.48021800953233 18.63743838566682 40-41 19.017709143309492 29.785470984345203 32.97563602392985 18.22118384841546 42-43 19.320352813554496 29.381890437159512 32.82262473225107 18.475132017034916 44-45 19.164072732895818 29.23006706746053 32.996787247244804 18.609072952398854 46-47 19.129930058344645 29.272844373083696 32.982516697090745 18.614708871480918 48-49 19.220748973983067 29.863174694971526 32.27657234470151 18.63950398634389 50-51 19.089617148383788 29.747812559698023 32.03885267310017 19.12371761881802 52-53 19.691188582878087 29.887524677843192 31.409232925151237 19.012053814127487 54-55 19.197297712392956 30.38382939299468 31.175390369911927 19.243482524700436 56-57 19.663288691165544 30.350382983032652 31.32076030406176 18.665568021740047 58-59 19.509860535735356 30.100189690500716 31.121446542386717 19.268503231377206 60-61 19.5760450293346 30.961175381343864 30.148060787277714 19.31471880204382 62-63 19.619155860408203 30.425141974667007 31.196239329279905 18.75946283564489 64-65 19.85686110738231 30.682850805617118 30.459892648375654 19.000395438624913 66-67 19.68496379588179 31.34311599868721 29.9252996528664 19.0466205525646 68-69 19.499596726006143 31.204996667403684 30.52098400310101 18.77442260348916 70-71 19.69895000317331 31.080490928659145 30.098700982357673 19.121858085809876 72-73 19.398273711503723 31.46788715336791 30.072471513871218 19.061367621257144 74-75 19.372340671370413 30.77331311137487 30.702812343267922 19.151533873986796 76-77 19.368194688304147 31.006201485360556 30.500583833263565 19.125019993071728 78-79 19.516957166547765 31.07439039709702 30.18660724806982 19.222045188285396 80-81 19.56370065378435 30.756260262861375 30.41158768199508 19.268451401359197 82-83 19.50503398101283 30.777758227873246 30.597649972390684 19.119557818723244 84-85 19.601913962947272 30.977844040222497 30.142399254384912 19.277842742445316 86-87 19.660720736271344 31.303001455214158 29.86633284769112 19.169944960823372 88-89 19.370755906143305 31.319553344581326 30.024569772105142 19.285120977170227 90-91 19.475939146250695 31.289814977528863 30.141819799665303 19.09242607655514 92-93 19.571215096874056 31.164764188983174 30.012320863139593 19.251699851003178 94-95 19.40540518103938 31.31041422464155 29.90881771022141 19.37536288409766 96-97 19.33525962523912 31.240528513672245 29.820160973159176 19.60405088792946 98-99 19.434201870961438 31.292024729848784 29.986901473367656 19.286871925822123 100-101 19.532392637805327 31.77859720548674 29.31408153897483 19.374928617733104 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9004.0 1 17090.5 2 22870.5 3 24527.0 4 33241.5 5 43379.5 6 53724.5 7 64524.5 8 75822.0 9 87218.5 10 98080.0 11 108309.0 12 118703.0 13 128240.5 14 136558.5 15 144065.0 16 152489.5 17 161585.0 18 173538.5 19 191157.5 20 215568.0 21 244746.5 22 285077.5 23 334740.5 24 401216.0 25 481493.5 26 573729.5 27 680078.5 28 797683.0 29 886746.5 30 985696.5 31 1099952.5 32 1207724.5 33 1273512.0 34 1254071.0 35 1278153.5 36 1319768.0 37 1361990.0 38 1435007.5 39 1357233.5 40 1269907.5 41 1228923.5 42 1119395.5 43 1057432.5 44 1014795.5 45 962725.5 46 914519.0 47 870799.0 48 825376.0 49 793159.5 50 735764.0 51 653436.0 52 589355.0 53 523694.0 54 457023.5 55 386428.5 56 330248.5 57 275676.0 58 221124.0 59 184696.0 60 147239.0 61 108425.0 62 81929.0 63 64246.5 64 44313.0 65 32020.0 66 25797.5 67 19861.5 68 14417.5 69 10827.0 70 12570.5 71 19307.5 72 19410.5 73 32000.0 74 34592.0 75 15735.5 76 8335.5 77 14626.5 78 13616.0 79 3379.0 80 1766.0 81 683.0 82 437.0 83 339.5 84 272.0 85 254.0 86 256.5 87 217.0 88 171.5 89 120.0 90 81.5 91 48.5 92 27.0 93 9.0 94 5.0 95 4.0 96 2.0 97 2.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07734291659154593 2 0.012282457991497707 3 0.0 4 0.02300348819485133 5 0.037796420232342774 6 0.12983068898668676 7 0.14895092368611657 8 0.15484545870046718 9 0.1782320481370193 10-11 0.17759499720858504 12-13 0.204394670434835 14-15 0.20447593433458972 16-17 0.20651333639272568 18-19 0.21739834553665577 20-21 0.22487897773729026 22-23 0.18670526082751493 24-25 0.20220489856465898 26-27 0.20844625629403415 28-29 0.2270353733629242 30-31 0.236562114468097 32-33 0.26609718860573317 34-35 0.2565994703219014 36-37 0.24560852788007678 38-39 0.2787511387103953 40-41 0.3020317948490529 42-43 0.3010609814751975 44-45 0.33872970129364005 46-47 0.32096047892763224 48-49 0.3081831818322711 50-51 0.30670737136708287 52-53 0.32382793367611995 54-55 0.32849625448881464 56-57 0.32189501377481144 58-59 0.32372490266035947 60-61 0.34332401391191525 62-63 0.3626677243356705 64-65 0.22086076812263328 66-67 0.1217463821021601 68-69 0.30633297697178435 70-71 0.05795422079471218 72-73 0.013923698538329454 74-75 0.028790638770240372 76-77 0.04524222504736815 78-79 0.0416491997653563 80-81 0.0506549812274587 82-83 0.07325360106460353 84-85 0.03521919369726481 86-87 0.007660573692948535 88-89 0.012086553947446172 90-91 0.007112042369604237 92-93 0.005482410951308877 94-95 0.012238923759486256 96-97 0.017017531293276652 98-99 0.0029182446858343437 100-101 0.005975798914105335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.4455644E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.74022006873281 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2989567093668 18.876529800044526 2 8.776369961667706 4.342586485125504 3 3.645462160888102 2.705686083378297 4 2.079028795447645 2.0574251971442896 5 1.4313847027420226 1.7706386274427666 6 1.0399110046889366 1.54365762647408 7 0.7652058900713491 1.3251953482779018 8 0.5939992141721192 1.1756537023418066 9 0.48667866972448204 1.0836483652567637 >10 3.915793985172707 19.61387161295758 >50 0.523672516569306 9.03130352837398 >100 0.389924734747227 18.76460242491176 >500 0.034101610271284055 5.717003429547313 >1k 0.018027128121159357 7.885120944616418 >5k 0.0010083831177990887 1.6500121228235127 >10k+ 4.7453323155602356E-4 2.4570647012834224 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG 103071 0.29914112184349245 TruSeq Adapter, Index 4 (100% over 49bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC 79759 0.23148312073342758 TruSeq Adapter, Index 4 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74969 0.21758118931110387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.35686461120854396 0.0 0.0 0.0 0.0 2 0.3600716329667209 0.0 0.0 2.9022821340968116E-6 0.0 3 0.3608146171930497 0.0 0.0 2.9022821340968116E-6 0.0 4 0.3712512237472618 0.0 0.0 2.9022821340968116E-6 0.0 5 0.3722931430334026 0.0 0.0 2.9022821340968116E-6 0.0 6 0.3748558581578101 0.0 0.0 2.9022821340968116E-6 0.0 7 0.3771979998400262 2.9022821340968116E-6 0.0 2.9022821340968116E-6 0.0 8 0.39272811153957826 2.9022821340968116E-6 0.0 5.804564268193623E-6 0.0 9 0.3938541970076078 2.9022821340968116E-6 0.0 5.804564268193623E-6 0.0 10-11 0.39996059861774746 2.9022821340968116E-6 0.0 5.804564268193623E-6 0.0 12-13 0.40526742149994355 2.9022821340968116E-6 0.0 5.804564268193623E-6 0.0 14-15 0.40893155269424075 8.706846402290435E-6 0.0 5.804564268193623E-6 0.0 16-17 0.41352731645358304 1.015798746933884E-5 0.0 5.804564268193623E-6 0.0 18-19 0.4183088262695075 1.3060269603435653E-5 0.0 8.706846402290435E-6 0.0 20-21 0.4449314602855776 1.4511410670484058E-5 0.0 8.706846402290435E-6 0.0 22-23 0.47025967646984046 1.5962551737532463E-5 0.0 8.706846402290435E-6 0.0 24-25 0.47455215174616966 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 26-27 0.4783019002634228 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 28-29 0.48168596123177965 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 30-31 0.4847696359992575 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 32-33 0.48825527684230774 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 34-35 0.492639174005861 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 36-37 0.49563723145038296 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 38-39 0.4988065235408167 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 40-41 0.5020178987221948 1.741369280458087E-5 0.0 8.706846402290435E-6 0.0 42-43 0.5051001223486057 1.8864833871629273E-5 0.0 1.1609128536387246E-5 1.4511410670484058E-6 44-45 0.5084159796868113 2.031597493867768E-5 2.9022821340968116E-6 1.1609128536387246E-5 2.9022821340968116E-6 46-47 0.5120307720848288 2.031597493867768E-5 2.9022821340968116E-6 1.1609128536387246E-5 4.353423201145217E-6 48-49 0.5154873901065382 2.031597493867768E-5 2.9022821340968116E-6 1.3060269603435653E-5 8.706846402290435E-6 50-51 0.5189773843727896 2.031597493867768E-5 2.9022821340968116E-6 1.4511410670484058E-5 8.706846402290435E-6 52-53 0.5231102341317435 2.031597493867768E-5 2.9022821340968116E-6 1.4511410670484058E-5 8.706846402290435E-6 54-55 0.5273809422920669 2.031597493867768E-5 2.9022821340968116E-6 1.8864833871629273E-5 8.706846402290435E-6 56-57 0.532065225656499 2.031597493867768E-5 2.9022821340968116E-6 2.031597493867768E-5 8.706846402290435E-6 58-59 0.5370905271716877 2.031597493867768E-5 2.9022821340968116E-6 2.031597493867768E-5 8.706846402290435E-6 60-61 0.5422609427935812 2.031597493867768E-5 2.9022821340968116E-6 2.1767116005726086E-5 8.706846402290435E-6 62-63 0.5478028505286391 2.031597493867768E-5 2.9022821340968116E-6 2.6120539206871302E-5 8.706846402290435E-6 64-65 0.5546261158259007 2.1767116005726086E-5 2.9022821340968116E-6 2.6120539206871302E-5 8.706846402290435E-6 66-67 0.5615683746906602 2.3218257072774492E-5 2.9022821340968116E-6 2.6120539206871302E-5 8.706846402290435E-6 68-69 0.5692695803334862 2.3218257072774492E-5 2.9022821340968116E-6 2.6120539206871302E-5 8.706846402290435E-6 70-71 0.5782245718582418 2.3218257072774492E-5 2.9022821340968116E-6 2.6120539206871302E-5 1.1609128536387246E-5 72-73 0.5882606634779486 2.3218257072774492E-5 2.9022821340968116E-6 2.6120539206871302E-5 1.1609128536387246E-5 74-75 0.5994779839262327 2.3218257072774492E-5 2.9022821340968116E-6 2.6120539206871302E-5 1.1609128536387246E-5 76-77 0.6127152927398484 2.3218257072774492E-5 2.9022821340968116E-6 2.9022821340968116E-5 1.1609128536387246E-5 78-79 0.6280495003953489 2.3218257072774492E-5 2.9022821340968116E-6 2.9022821340968116E-5 1.1609128536387246E-5 80-81 0.6454820580338014 2.3218257072774492E-5 2.9022821340968116E-6 3.627852667621014E-5 1.1609128536387246E-5 82-83 0.665346728100627 2.3218257072774492E-5 2.9022821340968116E-6 4.2083090944403765E-5 1.1609128536387246E-5 84-85 0.6885199998003231 2.3218257072774492E-5 2.9022821340968116E-6 4.353423201145217E-5 1.1609128536387246E-5 86-87 0.7160205741619574 2.3218257072774492E-5 2.9022821340968116E-6 4.498537307850058E-5 1.1609128536387246E-5 88-89 0.7484622838568915 2.3218257072774492E-5 2.9022821340968116E-6 4.93387962796458E-5 1.1609128536387246E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 18550 0.0 54.825836 1 GTTTTTT 575885 0.0 50.595146 1 GCGTGTT 25150 0.0 41.122658 2 AGATCGG 37415 0.0 31.59827 1 CGTGTTT 37740 0.0 27.655138 3 GATCGGA 44550 0.0 26.826868 2 ATCGGAA 45765 0.0 26.062225 3 TAAACGC 5170 0.0 24.32861 2 TCGGAAG 50850 0.0 23.44827 4 CGGAAGA 52845 0.0 22.564037 5 CTCGTAT 45650 0.0 21.933764 42-43 ATCTCGT 45200 0.0 21.867254 40-41 GCCGTCT 56005 0.0 21.30887 6 CGTCTGA 50175 0.0 21.29988 16-17 TATGCCG 56395 0.0 21.174955 3 ATGCCGT 56410 0.0 21.137112 4 CACGTCT 50830 0.0 20.851463 14-15 TGCCGTC 57315 0.0 20.81255 5 CCCCGCG 8955 0.0 20.732115 8 CGGAGAT 6310 0.0 20.692503 1 >>END_MODULE