FastQCFastQC Report
Fri 27 May 2016
SRR1233091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51304031
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4719450.9198984773730547Illumina Single End PCR Primer 1 (100% over 50bp)
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT3173720.6186102608584498No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT3115780.6073168012860432No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT2065260.4025531639024622No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA2059700.40146942839637695No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA1758560.34277228625563555No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1731590.3375153893852902No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT1565800.30520018982524005No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1543700.300892536105009No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1474880.28747838547033466No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA1459620.28450396032233805No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT1407400.27432542288928524No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA1304110.25419250194979803No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT1245870.24284056744001267No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT1235470.24081343627755097No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT1229860.23971995494856926No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT1189640.23188041501066456No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1173870.2288065824691241No Hit
ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA1135490.22132568881380882No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA1100410.21448801946965923No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC1059320.20647890221335627No Hit
ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT1047670.20420812547848338No Hit
GTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTA1013970.19763944084627583No Hit
ATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAAT971580.18937693219466517No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT930760.18142044238200308No Hit
CGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTC924200.18014179041798878No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG912490.17785931869564012Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG880060.1715381779650024No Hit
GTCGTATTAACCCTATAGTGAGTCGTATTAACCCTATAGTGAGTCGTATT877560.17105088682017988No Hit
ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT852830.16623060281559554No Hit
CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC834150.16258956338148167No Hit
ATTTAATACGACTCACTATAGGAGAATTTAATACGACTCACTATAGGAGA825370.16087819688086497No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT823750.16056243221901997No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT823100.1604357365213661No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT818400.15951962916909979No Hit
CTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACTCACTAT817450.15933445853406725No Hit
CGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACG770730.150227961619624No Hit
GTCGTATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCG758190.14778370923719425No Hit
CTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATACGACTCA744240.1450646246490846No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA740180.14427326382989283No Hit
ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG740090.14425572134867923No Hit
ATTTAATACGACTCACTATAGGATTTAATACGACTCACTATAGGATTTAA734270.1431213075635324No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT729530.1421974035529489No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG723340.14099087067836832No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC702670.13696194749297574No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA701790.13679042100999822No Hit
TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA698410.13613160338219818No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC672020.13098775805745166No Hit
CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT666540.12991961586800072No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG647340.12617721987576375No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA646020.12591993015129746No Hit
ATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATAC618300.12051684593750538No Hit
GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT614730.11982099418269881No Hit
CCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCC590290.11505723595091387No Hit
GTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGT586310.11428146844835643No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC586050.11423079016929488No Hit
GTCGTATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTA578680.1127942558743581No Hit
GGAATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGG577850.11263247521427704No Hit
ATACGACTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACT575740.11222120148804682No Hit
GTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTA564290.10998940804475968No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT559420.10904016489464541No Hit
ATTAATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAAT551390.10747498573747548No Hit
CTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAG550650.10733074755860803No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT548720.10695455879480503No Hit
CTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCT541430.10553361781650257No Hit
CCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATA521500.10164893280997746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG962600.060.5475351
AGAGCGT1032350.054.9958579
TCCGATC44100.054.080171
AAGAGCG1090050.053.2650228
CGCGTGT421400.051.490611
GATCGGA1147000.050.9635962
ATCGGAA1152850.050.9484943
CGGAAGA1153000.050.6851125
TCGGAAG1166450.050.2385524
GATCGGG35050.045.317181
GTTTTTT5157100.044.09341
GAAGAGC1324550.043.920957
CGCCGTA825000.043.1338746-47
CCGTATC834200.042.9297148-49
GCGTGTT502550.042.8836022
GTCGCCG813950.042.76900544-45
TGGTCGC790450.041.6179242-43
CCGATCT60700.041.580992
GGAAGAG1407650.041.50416
TCGGTGG879400.040.60952438-39
Produced by FastQC (version 0.11.5)