##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233091_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51304031 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.058490218829004 33.0 31.0 34.0 28.0 34.0 2 31.413845064143207 33.0 31.0 34.0 28.0 34.0 3 31.33118842065256 34.0 31.0 34.0 27.0 34.0 4 34.68290750876866 37.0 35.0 37.0 32.0 37.0 5 34.610230490465746 37.0 35.0 37.0 32.0 37.0 6 34.705902115176876 37.0 35.0 37.0 32.0 37.0 7 34.76359910198869 37.0 35.0 37.0 32.0 37.0 8 34.767970317965855 37.0 35.0 37.0 32.0 37.0 9 36.29262357571864 39.0 37.0 39.0 32.0 39.0 10-11 36.1924301620666 39.0 37.0 39.0 32.0 39.0 12-13 36.09617668249109 39.0 37.0 39.0 31.5 39.0 14-15 37.23637423343986 40.0 37.5 41.0 31.0 41.0 16-17 37.03881954226949 40.0 37.0 41.0 31.0 41.0 18-19 36.724220120247466 40.0 36.5 41.0 30.0 41.0 20-21 36.45716551005515 40.0 36.0 41.0 29.0 41.0 22-23 36.145656176607254 40.0 36.0 41.0 27.0 41.0 24-25 35.90447398762877 40.0 36.0 41.0 27.0 41.0 26-27 35.80670419445209 40.0 36.0 41.0 25.5 41.0 28-29 35.590406443891325 40.0 36.0 41.0 24.0 41.0 30-31 35.35245519011947 40.0 36.0 41.0 23.5 41.0 32-33 35.181591978610804 40.0 36.0 41.0 20.5 41.0 34-35 35.02045171655226 40.0 35.5 41.0 19.0 41.0 36-37 34.87081104796619 40.0 35.0 41.0 18.0 41.0 38-39 34.73709569721724 40.0 35.0 41.0 16.0 41.0 40-41 34.58826774254834 39.5 35.0 41.0 16.0 41.0 42-43 34.403958911142865 39.0 35.0 41.0 15.0 41.0 44-45 34.12087215914866 39.0 34.0 41.0 10.0 41.0 46-47 33.99839189049297 39.0 34.0 41.0 9.5 41.0 48-49 34.1225760408573 39.0 34.5 41.0 9.0 41.0 50-51 33.497223600227436 38.5 34.0 40.0 9.0 40.5 52-53 33.47819936410065 38.5 33.5 40.0 8.5 41.0 54-55 33.81155565534412 39.0 34.0 41.0 6.5 41.0 56-57 33.71798917710774 39.0 34.0 41.0 4.0 41.0 58-59 33.53057377694162 39.0 34.0 41.0 2.0 41.0 60-61 33.426463965375355 39.0 34.0 41.0 2.0 41.0 62-63 33.189323856443174 38.5 33.0 41.0 2.0 41.0 64-65 32.79553437038895 38.0 33.0 40.0 2.0 41.0 66-67 32.279799320252245 38.0 32.0 40.0 2.0 41.0 68-69 31.914940825994748 37.5 31.5 40.0 2.0 41.0 70-71 31.543968786780127 37.0 31.0 39.0 2.0 41.0 72-73 31.13391717699531 36.0 31.0 39.0 2.0 41.0 74-75 30.70432917834468 36.0 30.5 39.0 2.0 40.0 76-77 30.283216069318218 35.5 30.0 38.0 2.0 40.0 78-79 29.800115764782692 35.0 29.5 37.0 2.0 39.0 80-81 29.34387281381457 35.0 29.0 37.0 2.0 39.0 82-83 28.895151338108306 35.0 29.0 36.0 2.0 39.0 84-85 28.459406669234234 34.5 28.5 36.0 2.0 37.0 86-87 28.022365055486574 34.0 27.5 35.0 2.0 37.0 88-89 27.665508758951123 34.0 26.5 35.0 2.0 36.5 90-91 27.33796828752111 34.0 26.0 35.0 2.0 36.0 92-93 26.960747782177194 34.0 26.0 35.0 2.0 36.0 94-95 26.58533004940684 34.0 25.0 35.0 2.0 36.0 96-97 26.152925810059642 34.0 24.5 35.0 2.0 35.0 98-99 25.660199029974855 34.0 19.0 35.0 2.0 35.0 100-101 23.454842291047267 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6336748100102767 1101 2 0.6580331614835977 1101 3 0.7219860424306539 1101 4 0.7866977489473186 1101 5 0.7967938003635595 1101 6 0.6899719637027957 1101 7 0.6725193551511381 1101 8 0.6180889004988117 1101 9 0.7824534625715671 1101 10-11 0.741410260847772 1101 12-13 0.6712526605123657 1101 14-15 0.8612755714539091 1101 16-17 0.9558404120055641 1101 18-19 0.9261100360326253 1101 20-21 0.9921823503170302 1101 22-23 0.9828267988934769 1101 24-25 0.9457314479354224 1101 26-27 0.8658807514428872 1101 28-29 0.9690043516180609 1101 30-31 0.998186582448561 1101 32-33 0.9130053446084716 1101 34-35 0.9213855158241699 1101 36-37 0.9334214400673417 1101 38-39 0.9397775554950414 1101 40-41 1.0120211347901815 1101 42-43 1.0276124989702922 1101 44-45 1.0398336728663153 1101 46-47 0.9694653724585152 1101 48-49 0.9348999253125996 1101 50-51 0.9402086087921688 1101 52-53 1.0150168354678115 1101 54-55 1.093351156751588 1101 56-57 1.0774689365856887 1101 58-59 1.1302356308450072 1101 60-61 1.139346057994338 1101 62-63 1.1513003808974318 1101 64-65 1.0777922138123444 1101 66-67 1.1504171775133827 1101 68-69 1.0594967783091889 1101 70-71 0.9513791401127172 1101 72-73 0.8599730366953757 1101 74-75 0.995786736517946 1101 76-77 1.0598515895380167 1101 78-79 0.9971703315956262 1101 80-81 0.9797135030531514 1101 82-83 1.0502855235187596 1101 84-85 1.0588126196485597 1101 86-87 1.033228154720046 1101 88-89 1.1018197681817732 1101 90-91 1.056524119776391 1101 92-93 0.9358510806065574 1101 94-95 1.0653168048420945 1101 96-97 1.1272825479837785 1101 98-99 1.179690667030055 1101 100-101 1.3263828275892493 1102 1 0.6065480601372251 1102 2 0.6733131190686485 1102 3 0.7177515503518741 1102 4 0.6914233599571915 1102 5 0.7522475104820288 1102 6 0.6788846380124269 1102 7 0.6648288664552808 1102 8 0.6357448680667304 1102 9 0.7785066480274097 1102 10-11 0.7627651344399311 1102 12-13 0.8021840182590836 1102 14-15 0.9640870788380553 1102 16-17 0.9858111390338635 1102 18-19 0.9130259775915306 1102 20-21 0.906499596638561 1102 22-23 0.8482737417701358 1102 24-25 0.8906376893788917 1102 26-27 0.90947930210816 1102 28-29 0.9278615430525221 1102 30-31 0.9146473465825409 1102 32-33 0.8492918576037667 1102 34-35 0.8688345679387623 1102 36-37 0.8510810076079807 1102 38-39 0.900155726464881 1102 40-41 0.9496335279540844 1102 42-43 0.9456425356961304 1102 44-45 0.9823625327582022 1102 46-47 0.9753416763488829 1102 48-49 1.0095676722609639 1102 50-51 1.0319752901999166 1102 52-53 1.0563035061222408 1102 54-55 1.1749614961580832 1102 56-57 1.0797847027510912 1102 58-59 1.1384908016132158 1102 60-61 1.1077332050903408 1102 62-63 1.0834699234606475 1102 64-65 1.064300070272509 1102 66-67 1.1841356731875052 1102 68-69 1.149967826616269 1102 70-71 1.0891193759714746 1102 72-73 1.0721244830301622 1102 74-75 1.0809704475213273 1102 76-77 1.1168574436794003 1102 78-79 1.096315334161151 1102 80-81 1.021851615739493 1102 82-83 1.1091143925385119 1102 84-85 1.0962392167914103 1102 86-87 0.9974086953644807 1102 88-89 1.086224970663359 1102 90-91 1.0228281413581932 1102 92-93 1.0362485625177094 1102 94-95 1.1142277420144602 1102 96-97 1.0753604206939933 1102 98-99 1.077580471004186 1102 100-101 1.0200943287656017 1103 1 0.5152997612425096 1103 2 0.5864389338533265 1103 3 0.5649795980993098 1103 4 0.6261475660932803 1103 5 0.6526854292118784 1103 6 0.5854692597154809 1103 7 0.5637341817675292 1103 8 0.5902819071274834 1103 9 0.6717022806033981 1103 10-11 0.679113103737329 1103 12-13 0.7340803562872011 1103 14-15 0.8669136945679341 1103 16-17 0.874788198155521 1103 18-19 0.8548187176506659 1103 20-21 0.8180127225114617 1103 22-23 0.8555536586799732 1103 24-25 0.8765431786221214 1103 26-27 0.916365351004579 1103 28-29 0.8259984377385123 1103 30-31 0.8856503474889195 1103 32-33 0.9936499460761112 1103 34-35 0.9615769258242608 1103 36-37 0.9903666028811173 1103 38-39 0.9839519284317433 1103 40-41 0.9941651602604153 1103 42-43 0.9481861318538378 1103 44-45 0.9998741389339116 1103 46-47 0.9927628086431 1103 48-49 0.9267632639644106 1103 50-51 0.9815689542244925 1103 52-53 0.9776854995823854 1103 54-55 1.0481272740257523 1103 56-57 0.9815333182212598 1103 58-59 1.1517273285374188 1103 60-61 1.123682568466883 1103 62-63 1.077442184921786 1103 64-65 1.0678257836774776 1103 66-67 1.0843518130541412 1103 68-69 1.0607396359327694 1103 70-71 1.1280950593944574 1103 72-73 1.0836398598284909 1103 74-75 0.9977837179135847 1103 76-77 1.0651632562290878 1103 78-79 1.0154525971833017 1103 80-81 0.9380244583056587 1103 82-83 1.035786952451872 1103 84-85 0.984853652945354 1103 86-87 0.9904850127164444 1103 88-89 1.0335326512634673 1103 90-91 1.0141087984791959 1103 92-93 1.001130156650774 1103 94-95 0.990752560756949 1103 96-97 1.0183138327446493 1103 98-99 1.0776249577862416 1103 100-101 1.2186200778586453 1104 1 0.42811829930294465 1104 2 0.5323147353061195 1104 3 0.6213524219036621 1104 4 0.5773439816414054 1104 5 0.5299563388893631 1104 6 0.5341593170552557 1104 7 0.5266213735710465 1104 8 0.45113097661663915 1104 9 0.5433627538596753 1104 10-11 0.5829962073505044 1104 12-13 0.6114394123801574 1104 14-15 0.7010674724901378 1104 16-17 0.6931391959904829 1104 18-19 0.682455023672297 1104 20-21 0.6904089109491309 1104 22-23 0.6729668956046879 1104 24-25 0.6556940841606931 1104 26-27 0.6825170118973105 1104 28-29 0.6001205986147156 1104 30-31 0.6074195244907727 1104 32-33 0.7123659406511464 1104 34-35 0.6716917896679746 1104 36-37 0.6897976946400419 1104 38-39 0.6848948424950478 1104 40-41 0.674116107704755 1104 42-43 0.6704918927730077 1104 44-45 0.7319360452919099 1104 46-47 0.7510644338578842 1104 48-49 0.7526520422794931 1104 50-51 0.6415978514846188 1104 52-53 0.6881823677634955 1104 54-55 0.8210492025034739 1104 56-57 0.7525859274390356 1104 58-59 0.8847012064501314 1104 60-61 0.8311657235229504 1104 62-63 0.8052796385758185 1104 64-65 0.6646010267025133 1104 66-67 0.8139188918186449 1104 68-69 0.876815464744503 1104 70-71 0.9048927992471612 1104 72-73 0.7978560671063128 1104 74-75 0.8115529290759866 1104 76-77 0.8594976503789873 1104 78-79 0.8567318978600618 1104 80-81 0.8719594416792695 1104 82-83 0.835765446985441 1104 84-85 0.8514898134034006 1104 86-87 0.8105510671505876 1104 88-89 0.8255483962739589 1104 90-91 0.8751631822535906 1104 92-93 0.7928503559661344 1104 94-95 0.8055274826785563 1104 96-97 0.7977047402036099 1104 98-99 0.8690786655058425 1104 100-101 0.9927922392838617 1105 1 0.44700477388134985 1105 2 0.4351702460925999 1105 3 0.4074951678642087 1105 4 0.47127906252974583 1105 5 0.48623957713019905 1105 6 0.5134611394445869 1105 7 0.516694293564349 1105 8 0.4864616726366222 1105 9 0.561798659305154 1105 10-11 0.5497256138184454 1105 12-13 0.49526809162640006 1105 14-15 0.5399818201863482 1105 16-17 0.5675596978077024 1105 18-19 0.5064619767439282 1105 20-21 0.5667357620147939 1105 22-23 0.568280402911796 1105 24-25 0.5872382509937566 1105 26-27 0.6205100913842116 1105 28-29 0.6203947808464534 1105 30-31 0.5663589063017653 1105 32-33 0.5698709352471738 1105 34-35 0.6158052904823279 1105 36-37 0.5747455381552555 1105 38-39 0.6159824874469209 1105 40-41 0.6163630998260601 1105 42-43 0.6662653451862965 1105 44-45 0.6204853118843445 1105 46-47 0.6052892288914435 1105 48-49 0.6567869978452237 1105 50-51 0.5603243148813348 1105 52-53 0.580708514116246 1105 54-55 0.6788409508408151 1105 56-57 0.6478352222092312 1105 58-59 0.8136766554869297 1105 60-61 0.7742564081975658 1105 62-63 0.6807902850706071 1105 64-65 0.6924005320866513 1105 66-67 0.753599275433146 1105 68-69 0.7931256575437757 1105 70-71 0.7975050457087285 1105 72-73 0.7394434042668117 1105 74-75 0.7475866124099397 1105 76-77 0.724741310880848 1105 78-79 0.755899654027413 1105 80-81 0.6887994496009604 1105 82-83 0.6859507907313755 1105 84-85 0.6615546906225092 1105 86-87 0.7096947319434364 1105 88-89 0.7723922131650518 1105 90-91 0.7750527559536486 1105 92-93 0.7156574776988229 1105 94-95 0.7102853972977989 1105 96-97 0.7409723293233945 1105 98-99 0.7091879621144663 1105 100-101 0.7904034902785426 1106 1 0.38816732285382827 1106 2 0.4118996137794184 1106 3 0.46580904541089296 1106 4 0.4944565692013114 1106 5 0.4951004101662164 1106 6 0.4831895197588949 1106 7 0.40545531496375986 1106 8 0.3898779912725061 1106 9 0.49073038810885095 1106 10-11 0.46793370712396154 1106 12-13 0.47728267383651257 1106 14-15 0.5107451534032563 1106 16-17 0.5119618477167265 1106 18-19 0.5190447685866317 1106 20-21 0.5193703737556135 1106 22-23 0.5009301135829318 1106 24-25 0.5221650010634065 1106 26-27 0.5368191205740445 1106 28-29 0.49163181174441917 1106 30-31 0.5554089753013756 1106 32-33 0.6257548539990907 1106 34-35 0.5890072803993291 1106 36-37 0.4993947907787586 1106 38-39 0.599856242092109 1106 40-41 0.5636728550882495 1106 42-43 0.5577140470054545 1106 44-45 0.5282261416417029 1106 46-47 0.5282474893931735 1106 48-49 0.575502805146229 1106 50-51 0.5010403550756095 1106 52-53 0.5890798952823957 1106 54-55 0.747755962700424 1106 56-57 0.6384041222163361 1106 58-59 0.7863640195536732 1106 60-61 0.7403802301874762 1106 62-63 0.6818750079615725 1106 64-65 0.6593427462570531 1106 66-67 0.6805484881155337 1106 68-69 0.7122941414509185 1106 70-71 0.7777747459854218 1106 72-73 0.6615936007874659 1106 74-75 0.6823284724565148 1106 76-77 0.6847343923581626 1106 78-79 0.741166884032058 1106 80-81 0.7757174012109651 1106 82-83 0.7280765406158061 1106 84-85 0.6935695291228363 1106 86-87 0.7069546510529428 1106 88-89 0.7275537176525866 1106 90-91 0.7142320949805026 1106 92-93 0.7352459366883224 1106 94-95 0.7276626169222666 1106 96-97 0.6470298859155044 1106 98-99 0.696975412438384 1106 100-101 0.6981199261251732 1107 1 0.35122299194448914 1107 2 0.37352288532073175 1107 3 0.37611930933857707 1107 4 0.4091467132229667 1107 5 0.4199185570349684 1107 6 0.4269055571669398 1107 7 0.3683872475156207 1107 8 0.3715470369961196 1107 9 0.41156874957967915 1107 10-11 0.3965022277843886 1107 12-13 0.385487979068742 1107 14-15 0.4582675143692754 1107 16-17 0.44758936024756935 1107 18-19 0.3886893424006601 1107 20-21 0.43502964757082196 1107 22-23 0.4497897729555902 1107 24-25 0.41850349061737546 1107 26-27 0.3930258180467163 1107 28-29 0.4415357720518571 1107 30-31 0.39692513051612366 1107 32-33 0.4101925393813062 1107 34-35 0.42558815111085124 1107 36-37 0.34727605488943425 1107 38-39 0.4107998117550338 1107 40-41 0.4257874295705335 1107 42-43 0.38814898122264907 1107 44-45 0.4348086891545222 1107 46-47 0.4670895488276372 1107 48-49 0.43820529128664276 1107 50-51 0.386439604571855 1107 52-53 0.38550429783411033 1107 54-55 0.4936073356506867 1107 56-57 0.4280228265381467 1107 58-59 0.5197111341280021 1107 60-61 0.46741389083216944 1107 62-63 0.49587871570303577 1107 64-65 0.4151149598756376 1107 66-67 0.45369015503150933 1107 68-69 0.4937454425956176 1107 70-71 0.4978006391127927 1107 72-73 0.5157568508784145 1107 74-75 0.5770062909040377 1107 76-77 0.579085797911997 1107 78-79 0.5516674619926683 1107 80-81 0.5102919681662677 1107 82-83 0.5637627687123299 1107 84-85 0.5915939267921075 1107 86-87 0.5520807507839933 1107 88-89 0.509223892521689 1107 90-91 0.5727663603615198 1107 92-93 0.578837446751006 1107 94-95 0.6322358828105656 1107 96-97 0.6648384214006882 1107 98-99 0.571681419375853 1107 100-101 0.4644143576361124 1108 1 0.15341122932892404 1108 2 0.2752733527532101 1108 3 0.33765075646816456 1108 4 0.28096067277042636 1108 5 0.26849230887328446 1108 6 0.28847266358921786 1108 7 0.23625289051614828 1108 8 0.24582006141362456 1108 9 0.23225940545270873 1108 10-11 0.259815006675268 1108 12-13 0.22676290962974122 1108 14-15 0.2249303632843649 1108 16-17 0.2744628305906005 1108 18-19 0.23273627157207244 1108 20-21 0.2512039670829793 1108 22-23 0.2247120378165448 1108 24-25 0.25488303768209164 1108 26-27 0.32785310131320244 1108 28-29 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2316 96-97 -3.290916496336802 2316 98-99 -3.2691786601211597 2316 100-101 -2.958962739913474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 608343.0 3 159260.0 4 129618.0 5 134134.0 6 173675.0 7 262773.0 8 416130.0 9 647098.0 10 708211.0 11 543059.0 12 405193.0 13 331079.0 14 294366.0 15 275213.0 16 271407.0 17 275832.0 18 289182.0 19 309119.0 20 337821.0 21 372201.0 22 419756.0 23 480526.0 24 574637.0 25 555788.0 26 524465.0 27 592575.0 28 672790.0 29 780066.0 30 914076.0 31 1090885.0 32 1332911.0 33 1676790.0 34 2201173.0 35 3089928.0 36 4685362.0 37 8451584.0 38 1.3076658E7 39 3234651.0 40 5696.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.69070523779991 24.288910057645694 8.601019168527696 41.4193655360267 2 14.276635681065914 14.3397460993164 60.403058323909576 10.980559895708108 3 12.676008378689229 27.07076028238591 36.36206281827643 23.891168520648435 4 18.167721484277955 30.88368365219839 30.899420331433742 20.04917453208991 5 17.243268425359346 31.62068810837483 34.297226080033134 16.83881738623269 6 14.003295472158769 40.835226685061876 30.986259555106376 14.175218287672978 7 11.01827461679892 23.18116861916737 50.058026981941794 15.742529782091925 8 11.272175245833346 22.65056883870269 47.503396641700405 18.573859273763567 9 12.946088327160442 26.956804200919404 37.60538985414543 22.49171761777472 10-11 20.67629232283342 33.74740691099189 28.71717319431109 16.859127571863603 12-13 18.103488793494076 25.994186888746572 37.670742217927774 18.231582099831577 14-15 18.318875595743265 27.902255294294996 37.58492639053537 16.193942719426367 16-17 20.707498783434954 26.962950109800566 36.48580259863326 15.843748508131208 18-19 19.620079386277492 30.203620107054956 36.12892417787951 14.047376328788038 20-21 20.803147240395468 29.907199954790986 33.41800721724575 15.871645587567787 22-23 21.893284352641512 29.389854852648966 32.1990077931463 16.51785300156322 24-25 17.332507785390018 31.145111283557718 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16.677205226886592 30.898159002998106 33.874185859161194 18.550449910954107 52-53 16.89646356070985 31.699782566768665 32.79484439270142 18.608909479820067 54-55 17.18728437932002 32.018528677720894 31.01361124688122 19.780575696077868 56-57 17.867806495042394 31.592298346183906 32.58811398044121 17.95178117833249 58-59 18.591381877150408 30.970277038311494 32.25200085830636 18.186340226231735 60-61 18.92295929297308 33.71814064805469 29.55066264389567 17.808237415076555 62-63 18.95313683755388 32.96396930685936 31.008711874018985 17.07418198156778 64-65 20.20813392416837 30.01777166575852 31.850541343657113 17.923553066416005 66-67 18.95220105221944 34.78891077476287 28.109413058399774 18.149475114617925 68-69 19.35551180873496 31.26978560568255 32.63090633284907 16.74379625273342 70-71 19.8339421678868 30.77139648017238 30.842470404654133 18.552190947286686 72-73 17.297433857935754 35.07780699177107 29.427708375147983 18.19705077514519 74-75 17.427646102901644 30.374885251863752 33.40748438772557 18.789984257509033 76-77 18.294962528719843 32.25929774825765 30.94533184417952 18.500407878842985 78-79 17.696199791875983 33.23039519105466 30.619937949904386 18.453467067164965 80-81 18.561453375602017 31.037726796788263 31.81069827892627 18.59012154868345 82-83 19.835926947886033 31.498628548963946 30.28349721399557 18.38194728915445 84-85 19.090173042397105 31.745914209129246 30.670195373148207 18.493717375325446 86-87 19.67726117844258 30.7525131360464 30.8782949571459 18.69193072836512 88-89 19.28169310477861 31.508519814592894 30.711916625644914 18.497870454983584 90-91 18.849265821066886 31.64010057959142 31.83935501747926 17.67127858186243 92-93 19.55650749806027 31.62575788570315 30.470332019721397 18.34740259651518 94-95 19.35372644428431 31.469660093005192 30.92705533727651 18.24955812543399 96-97 18.22873518688236 31.96417298195356 30.17820421578618 19.628887615377895 98-99 18.452866771366747 31.98843207828373 31.533536151321385 18.02516499902814 100-101 18.674200789930516 32.77902173454811 30.049304045529666 18.497473429991707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22402.0 1 26437.0 2 26468.0 3 25334.0 4 29941.0 5 36200.0 6 42892.5 7 49323.5 8 58631.0 9 67084.0 10 73393.5 11 80054.5 12 88787.0 13 96617.5 14 105375.0 15 115714.0 16 125299.0 17 137189.0 18 155494.0 19 171960.5 20 191574.0 21 223095.0 22 261424.0 23 312233.0 24 398530.0 25 475886.0 26 542014.0 27 657227.5 28 983444.0 29 1243419.5 30 1535334.0 31 1893019.5 32 2763947.5 33 3211536.5 34 2628783.0 35 2796763.0 36 3070139.5 37 3185602.5 38 3505080.0 39 2981691.5 40 2403893.0 41 2112831.0 42 1611573.5 43 1325939.5 44 1109326.0 45 957153.0 46 877403.0 47 805577.0 48 757161.0 49 735117.5 50 649086.5 51 531027.0 52 471947.0 53 432800.0 54 391672.0 55 339052.5 56 288172.0 57 238687.0 58 197059.5 59 166798.0 60 134115.5 61 99243.0 62 70866.5 63 50777.0 64 35235.0 65 24560.0 66 17913.5 67 13262.5 68 11192.0 69 8360.5 70 6351.0 71 6680.0 72 6025.0 73 7231.5 74 7520.5 75 4644.5 76 3185.0 77 3052.5 78 2824.5 79 1697.5 80 949.0 81 599.0 82 374.5 83 211.5 84 169.0 85 148.0 86 151.0 87 113.5 88 54.0 89 29.0 90 19.0 91 20.0 92 15.0 93 9.0 94 7.0 95 4.0 96 2.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6423218479655136 2 0.2847242159197978 3 0.2839367534297646 4 0.3040891660150447 5 0.27599390776915755 6 0.21352318300291062 7 0.09466117779322253 8 0.10697210127601864 9 0.10286521150745447 10-11 0.08713350418800425 12-13 0.09591059228854747 14-15 0.09518842681192048 16-17 0.09163022687242646 18-19 0.10670798947552483 20-21 0.11229526974205985 22-23 0.08861974217971294 24-25 0.10531238763675314 26-27 0.09041589733952873 28-29 0.09788119767820973 30-31 0.11006640004564165 32-33 0.10567688141308038 34-35 0.10867762028289746 36-37 0.10800905683220095 38-39 0.10888130798143328 40-41 0.1032365273598092 42-43 0.09283578516471737 44-45 0.3741060814500131 46-47 0.08603125161841571 48-49 0.07188226593734905 50-51 0.07471342748876789 52-53 0.2295004850593514 54-55 0.3010591896765383 56-57 0.22491215163970257 58-59 0.2601959288540115 60-61 0.07479626698338772 62-63 0.06717795722523245 64-65 0.10563010146317743 66-67 0.10095503021975018 68-69 0.08066617611391978 70-71 0.10911715689552737 72-73 0.13351874826365984 74-75 0.12174092129329955 76-77 0.1102886048076807 78-79 0.10209236775176593 80-81 0.09809950411109021 82-83 0.10512429325485165 84-85 0.0914362849967871 86-87 0.12032095489728671 88-89 0.1285932093717938 90-91 0.15461748025218525 92-93 0.1616188794209952 94-95 0.11445299493133396 96-97 0.09969392073694949 98-99 0.11609711525396513 100-101 0.10837257602623857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.1304031E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.561112401648863 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2574474471459 13.291526082416096 2 7.573254903157175 2.508430513950487 3 2.959964289485333 1.470609039090999 4 1.687073161386041 1.1175923302207726 5 1.1231187408610048 0.930004785389872 6 0.7808697520136371 0.7759243040487308 7 0.5827111744268965 0.6755241680166454 8 0.4558700088295475 0.6039771565413435 9 0.39635054720614193 0.5907605366462355 >10 3.1556854495775952 10.863354581100198 >50 0.49754805705253224 5.792298903046918 >100 0.4368320061777583 14.507099479527529 >500 0.050021559464254545 5.697338515353093 >1k 0.03525662816494795 11.545262807958016 >5k 0.003734787150195338 4.205951625492647 >10k+ 0.0042614879009507745 25.424345171200446 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 471945 0.9198984773730547 Illumina Single End PCR Primer 1 (100% over 50bp) ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 317372 0.6186102608584498 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 311578 0.6073168012860432 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 206526 0.4025531639024622 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 205970 0.40146942839637695 No Hit ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA 175856 0.34277228625563555 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 173159 0.3375153893852902 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 156580 0.30520018982524005 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 154370 0.300892536105009 No Hit CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT 147488 0.28747838547033466 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 145962 0.28450396032233805 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 140740 0.27432542288928524 No Hit ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA 130411 0.25419250194979803 No Hit GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT 124587 0.24284056744001267 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 123547 0.24081343627755097 No Hit CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT 122986 0.23971995494856926 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 118964 0.23188041501066456 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 117387 0.2288065824691241 No Hit ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA 113549 0.22132568881380882 No Hit ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA 110041 0.21448801946965923 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 105932 0.20647890221335627 No Hit ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT 104767 0.20420812547848338 No Hit GTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTA 101397 0.19763944084627583 No Hit ATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAAT 97158 0.18937693219466517 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 93076 0.18142044238200308 No Hit CGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTC 92420 0.18014179041798878 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 91249 0.17785931869564012 Illumina Single End PCR Primer 1 (100% over 50bp) CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 88006 0.1715381779650024 No Hit GTCGTATTAACCCTATAGTGAGTCGTATTAACCCTATAGTGAGTCGTATT 87756 0.17105088682017988 No Hit ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT 85283 0.16623060281559554 No Hit CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC 83415 0.16258956338148167 No Hit ATTTAATACGACTCACTATAGGAGAATTTAATACGACTCACTATAGGAGA 82537 0.16087819688086497 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 82375 0.16056243221901997 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 82310 0.1604357365213661 No Hit ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT 81840 0.15951962916909979 No Hit CTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACTCACTAT 81745 0.15933445853406725 No Hit CGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACG 77073 0.150227961619624 No Hit GTCGTATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCG 75819 0.14778370923719425 No Hit CTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATACGACTCA 74424 0.1450646246490846 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 74018 0.14427326382989283 No Hit ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG 74009 0.14425572134867923 No Hit ATTTAATACGACTCACTATAGGATTTAATACGACTCACTATAGGATTTAA 73427 0.1431213075635324 No Hit CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT 72953 0.1421974035529489 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 72334 0.14099087067836832 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 70267 0.13696194749297574 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 70179 0.13679042100999822 No Hit TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA 69841 0.13613160338219818 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 67202 0.13098775805745166 No Hit CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT 66654 0.12991961586800072 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 64734 0.12617721987576375 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 64602 0.12591993015129746 No Hit ATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATAC 61830 0.12051684593750538 No Hit GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT 61473 0.11982099418269881 No Hit CCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCC 59029 0.11505723595091387 No Hit GTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGT 58631 0.11428146844835643 No Hit CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC 58605 0.11423079016929488 No Hit GTCGTATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTA 57868 0.1127942558743581 No Hit GGAATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGG 57785 0.11263247521427704 No Hit ATACGACTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACT 57574 0.11222120148804682 No Hit GTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTA 56429 0.10998940804475968 No Hit ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT 55942 0.10904016489464541 No Hit ATTAATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAAT 55139 0.10747498573747548 No Hit CTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAG 55065 0.10733074755860803 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 54872 0.10695455879480503 No Hit CTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCT 54143 0.10553361781650257 No Hit CCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATA 52150 0.10164893280997746 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1723367312014918 0.0 0.0 1.9491645792900756E-6 0.0 2 1.1748979334586789 1.9491645792900756E-6 0.0 1.9491645792900756E-6 0.0 3 1.1749739508772712 1.9491645792900756E-6 0.0 1.9491645792900756E-6 0.0 4 1.19394906805666 1.9491645792900756E-6 0.0 1.9491645792900756E-6 0.0 5 1.1943817825932626 1.9491645792900756E-6 0.0 3.898329158580151E-6 0.0 6 1.1947131405717417 1.9491645792900756E-6 0.0 3.898329158580151E-6 0.0 7 1.196019080839866 1.9491645792900756E-6 0.0 3.898329158580151E-6 0.0 8 1.2035077711534987 1.9491645792900756E-6 0.0 3.898329158580151E-6 0.0 9 1.2037806541945992 1.9491645792900756E-6 0.0 5.847493737870227E-6 0.0 10-11 1.208479115412978 1.9491645792900756E-6 0.0 5.847493737870227E-6 0.0 12-13 1.2112488782801492 1.9491645792900756E-6 0.0 5.847493737870227E-6 0.0 14-15 1.2134689767359605 1.9491645792900756E-6 0.0 8.771240606805341E-6 0.0 16-17 1.2152466148322731 1.9491645792900756E-6 0.0 1.1694987475740454E-5 0.0 18-19 1.217251330602073 1.9491645792900756E-6 0.0 1.2669569765385493E-5 0.0 20-21 1.2197969395426258 1.9491645792900756E-6 0.0 1.364415205503053E-5 0.0 22-23 1.2223522943060752 4.872911448225189E-6 0.0 1.5593316634320608E-5 0.0 24-25 1.22456264693899 5.847493737870227E-6 0.0 1.9491645792900757E-5 0.0 26-27 1.2267174483813954 5.847493737870227E-6 0.0 2.338997495148091E-5 0.0 28-29 1.2291938619793834 5.847493737870227E-6 0.0 2.5339139530770983E-5 0.0 30-31 1.232033794771409 1.364415205503053E-5 0.0 2.5339139530770983E-5 0.0 32-33 1.2352421196689205 1.7542481213610682E-5 0.0 2.6313721820416024E-5 0.0 34-35 1.238930913635227 1.851706350325572E-5 0.0 2.728830411006106E-5 0.0 36-37 1.2423575449656188 1.9491645792900757E-5 0.0 2.728830411006106E-5 0.0 38-39 1.2462548995419094 1.9491645792900757E-5 0.0 2.9237468689351135E-5 0.0 40-41 1.25064539275676 1.9491645792900757E-5 0.0 3.0212050978996172E-5 0.0 42-43 1.2554081764062555 1.9491645792900757E-5 0.0 3.8008709296156476E-5 0.0 44-45 1.261372620018883 1.9491645792900757E-5 0.0 4.288162074438166E-5 0.0 46-47 1.2758159295514226 1.9491645792900757E-5 0.0 4.872911448225189E-5 1.9491645792900756E-6 48-49 1.2826224122623036 1.9491645792900757E-5 0.0 4.872911448225189E-5 1.9491645792900756E-6 50-51 1.2886882124330543 1.9491645792900757E-5 0.0 5.262744364083205E-5 1.9491645792900756E-6 52-53 1.2952627445589997 2.144081037219083E-5 0.0 5.555119050976716E-5 2.923746868935113E-6 54-55 1.3023460086401397 2.144081037219083E-5 0.0 5.6525772799412196E-5 3.898329158580151E-6 56-57 1.310313218857988 2.3389974951480905E-5 0.0 5.847493737870227E-5 3.898329158580151E-6 58-59 1.3185757275095986 2.5339139530770983E-5 9.745822896450378E-7 6.334784882692745E-5 3.898329158580151E-6 60-61 1.3275399354097537 2.728830411006106E-5 1.9491645792900756E-6 6.822076027515264E-5 3.898329158580151E-6 62-63 1.337129825139861 2.728830411006106E-5 1.9491645792900756E-6 7.016992485444273E-5 3.898329158580151E-6 64-65 1.347680653007558 2.728830411006106E-5 1.9491645792900756E-6 7.406825401302288E-5 3.898329158580151E-6 66-67 1.3593619963312435 2.728830411006106E-5 1.9491645792900756E-6 7.894116546124807E-5 4.872911448225189E-6 68-69 1.3727205957754078 2.728830411006106E-5 1.9491645792900756E-6 7.99157477508931E-5 5.847493737870227E-6 70-71 1.3865489438831813 2.728830411006106E-5 1.9491645792900756E-6 8.381407690947325E-5 5.847493737870227E-6 72-73 1.4012349244058426 2.9237468689351135E-5 1.9491645792900756E-6 8.966157064734349E-5 5.847493737870227E-6 74-75 1.418077655535488 3.118663326864121E-5 1.9491645792900756E-6 9.355989980592364E-5 5.847493737870227E-6 76-77 1.4363881075933391 3.118663326864121E-5 1.9491645792900756E-6 1.081786341505992E-4 5.847493737870227E-6 78-79 1.4562598404792013 3.216121555828625E-5 1.9491645792900756E-6 1.1500071017811447E-4 5.847493737870227E-6 80-81 1.4791362105640393 3.3135797847931284E-5 1.9491645792900756E-6 1.2279736849527478E-4 5.847493737870227E-6 82-83 1.5033584787908771 3.3135797847931284E-5 1.9491645792900756E-6 1.2572111536420988E-4 5.847493737870227E-6 84-85 1.5317928916735606 3.3135797847931284E-5 1.9491645792900756E-6 1.28644862233145E-4 5.847493737870227E-6 86-87 1.563649062975188 3.3135797847931284E-5 1.9491645792900756E-6 1.3254319139172514E-4 5.847493737870227E-6 88-89 1.6002144159003802 3.3135797847931284E-5 1.9491645792900756E-6 1.3644152055030528E-4 5.847493737870227E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 96260 0.0 60.547535 1 AGAGCGT 103235 0.0 54.995857 9 TCCGATC 4410 0.0 54.08017 1 AAGAGCG 109005 0.0 53.265022 8 CGCGTGT 42140 0.0 51.49061 1 GATCGGA 114700 0.0 50.963596 2 ATCGGAA 115285 0.0 50.948494 3 CGGAAGA 115300 0.0 50.685112 5 TCGGAAG 116645 0.0 50.238552 4 GATCGGG 3505 0.0 45.31718 1 GTTTTTT 515710 0.0 44.0934 1 GAAGAGC 132455 0.0 43.92095 7 CGCCGTA 82500 0.0 43.13387 46-47 CCGTATC 83420 0.0 42.92971 48-49 GCGTGTT 50255 0.0 42.883602 2 GTCGCCG 81395 0.0 42.769005 44-45 TGGTCGC 79045 0.0 41.61792 42-43 CCGATCT 6070 0.0 41.58099 2 GGAAGAG 140765 0.0 41.5041 6 TCGGTGG 87940 0.0 40.609524 38-39 >>END_MODULE