FastQCFastQC Report
Fri 27 May 2016
SRR1233091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51304031
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG5131211.000157278089903TruSeq Adapter, Index 10 (100% over 49bp)
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT3234100.6303793165882035No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT2930930.5712864940378661No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2347820.45762875825488253TruSeq Adapter, Index 10 (100% over 50bp)
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1978830.38570653444365804No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1901760.3706843230310694No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA1715720.33442206519795686No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1572270.3064612993080407No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1545080.301161520816951No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT1442740.28121377051249635No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA1413280.2754715316619078No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA1387610.27046802618687016No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT1314990.2563131930120656No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1301400.25366427834881045No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT1260760.24574287349857557No Hit
ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA1204810.2348372976774476No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA1156530.22542673108863512No Hit
GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT1149890.22413248580798653No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT1096770.21377852356279764No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT1087740.21201842794769868No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1053510.20534643759278876No Hit
ATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAAT1034510.20164302489213762No Hit
ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT1025080.19980496269386708No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC1016030.19804096874960955No Hit
GTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTA935520.18234824472174516No Hit
ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT922500.17981043243950948No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT863760.16836103970075958No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT852190.16610585628252095No Hit
CGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTC850680.16581153243104815No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT837410.16322499103433022No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG806790.157256649092544No Hit
GTCGTATTAACCCTATAGTGAGTCGTATTAACCCTATAGTGAGTCGTATT801410.15620799854888595No Hit
CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC765680.14924363350708253No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT761120.14835481445892623No Hit
TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA758290.14780320088298715No Hit
CTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACTCACTAT752110.1465986171729859No Hit
ATTTAATACGACTCACTATAGGAGAATTTAATACGACTCACTATAGGAGA741270.14448572276903543No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT727620.14182511311830448No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG726190.141546382583466No Hit
CGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACG724440.14120527878209024No Hit
ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG713740.13911967268224987No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA711120.13860899156247586No Hit
GTCGTATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCG709050.1382055144945628No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA673760.13132691269424815No Hit
ATTTAATACGACTCACTATAGGATTTAATACGACTCACTATAGGATTTAA665980.12981046265156046No Hit
CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT663550.12933681565879296No Hit
CTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATACGACTCA663390.12930562902552434No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC642930.12531763829629683No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG609890.11887759852632243No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA602850.11750538666250221No Hit
ATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATAC602460.1174293692439099No Hit
CCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCC598520.11666139839966962No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC587570.11452706318534699No Hit
ATACGACTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACT581600.11336341193151081No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC577990.11265976351838708No Hit
GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT577780.112618831062222No Hit
GTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGT569990.11110043185495504No Hit
ATTAATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAAT564810.11009076460288275No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT558030.10876923101812408No Hit
GTCGTATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTA551620.10751981652279916No Hit
GGAATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGG549310.10706955950498315No Hit
GTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTA547590.10673430319734527No Hit
CTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAG544010.10603650227795941No Hit
CTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCT535110.10430174580239124No Hit
CCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATA533320.1039528453426983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAT222250.062.9170071
AGATCGG933950.061.9072041
CGCGTGT420900.053.8326841
GATCGGA1237400.047.9633372
ATCGGAA1267500.046.800673
CGGAAGA1272300.046.5465855
TCGGAAG1298250.045.6336254
GCGTGTT501650.045.5450362
AGAGCAC1301450.045.2323729
CCGTCTT1002100.042.63478550-51
TATGCCG1022050.042.49665546-47
TGCCGTC1001050.042.41861748-49
GTTTTTT4642550.041.3036081
GAAGAGC1439050.040.968717
GGAAGAG1464050.040.4441036
ATCTCGT1041200.040.419940-41
CTCGTAT1066150.040.1678542-43
ACTAGCT1130350.039.87158632-33
GCTTATC1074450.039.69059436-37
AAGAGCA1495300.039.484818
Produced by FastQC (version 0.11.5)