##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233091_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51304031 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.233765140208963 31.0 31.0 34.0 26.0 34.0 2 31.212178922159158 33.0 31.0 34.0 27.0 34.0 3 31.381903149091734 34.0 31.0 34.0 28.0 34.0 4 34.822154383931355 37.0 35.0 37.0 32.0 37.0 5 34.60621634194787 37.0 35.0 37.0 32.0 37.0 6 34.640716730426114 37.0 35.0 37.0 32.0 37.0 7 34.66018013282426 37.0 35.0 37.0 32.0 37.0 8 34.60823259677198 37.0 35.0 37.0 32.0 37.0 9 36.175223365976834 39.0 37.0 39.0 32.0 39.0 10-11 36.057041521747095 39.0 37.0 39.0 31.5 39.0 12-13 35.96976274632299 39.0 37.0 39.0 31.0 39.0 14-15 37.18051012404854 40.0 38.0 41.0 31.0 41.0 16-17 37.034103577553196 40.0 37.5 41.0 31.0 41.0 18-19 36.78107976544767 40.0 37.0 41.0 30.5 41.0 20-21 36.55883213153368 40.0 37.0 41.0 30.0 41.0 22-23 36.34631668610991 40.0 37.0 41.0 29.5 41.0 24-25 36.095135779876635 40.0 36.0 41.0 27.5 41.0 26-27 35.84227385563524 40.0 36.0 41.0 27.0 41.0 28-29 35.60170962004915 40.0 36.0 41.0 24.5 41.0 30-31 35.29652409768737 40.0 36.0 41.0 22.5 41.0 32-33 35.20770115510027 40.0 36.0 41.0 22.0 41.0 34-35 35.24174625771609 40.0 36.0 41.0 21.5 41.0 36-37 35.15574794697906 40.0 36.0 41.0 19.5 41.0 38-39 35.0639216536416 40.0 36.0 41.0 18.5 41.0 40-41 34.829103672185134 40.0 35.0 41.0 15.5 41.0 42-43 34.826219892935896 40.0 35.0 41.0 16.0 41.0 44-45 34.762319436459094 40.0 35.0 41.0 15.0 41.0 46-47 34.64441184943148 40.0 35.0 41.0 14.5 41.0 48-49 34.596444487958465 40.0 35.0 41.0 12.5 41.0 50-51 34.50135961051481 40.0 35.0 41.0 9.0 41.0 52-53 34.354467234748086 40.0 35.0 41.0 8.5 41.0 54-55 34.190632993341204 39.5 35.0 41.0 7.5 41.0 56-57 34.06541575651239 39.0 34.5 41.0 6.0 41.0 58-59 34.01309352280721 39.0 34.0 41.0 2.0 41.0 60-61 33.91535133954679 39.0 34.0 41.0 2.0 41.0 62-63 33.73527920252504 39.0 34.0 41.0 2.0 41.0 64-65 33.24285665779361 39.0 34.0 40.5 2.0 41.0 66-67 33.05391714736801 38.0 34.0 40.0 2.0 41.0 68-69 32.625601222250936 37.5 33.0 40.0 2.0 41.0 70-71 32.212569018212236 37.0 32.5 39.5 2.0 41.0 72-73 31.745877628991764 37.0 32.0 39.0 2.0 41.0 74-75 31.37270794569729 36.0 32.0 39.0 2.0 41.0 76-77 30.145969163709573 35.0 30.5 37.0 2.0 39.0 78-79 30.575965765730963 35.0 31.0 37.0 2.0 39.0 80-81 30.370660786868775 35.0 31.0 37.0 2.0 39.0 82-83 30.011853279131223 35.0 31.0 37.0 2.0 39.0 84-85 29.662365614117142 35.0 31.0 36.0 2.0 37.5 86-87 29.292619219335805 35.0 30.5 36.0 2.0 37.0 88-89 29.00664331814395 35.0 30.0 35.5 2.0 37.0 90-91 28.745348421062666 35.0 30.0 35.0 2.0 36.0 92-93 28.483609806410726 35.0 29.5 35.0 2.0 36.0 94-95 28.233290791516946 34.0 29.0 35.0 2.0 36.0 96-97 27.95055430439764 34.0 29.0 35.0 2.0 36.0 98-99 27.657356641235463 34.0 29.0 35.0 2.0 35.5 100-101 26.538259839660554 33.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5294604412513877 1101 2 0.9219220562746848 1101 3 0.8587665341674118 1101 4 0.7580221253691732 1101 5 0.7609071802418015 1101 6 0.7533706010746357 1101 7 0.7395362714212439 1101 8 0.7515460864427723 1101 9 0.8892925989264313 1101 10-11 0.7876547206788658 1101 12-13 0.8875965818943072 1101 14-15 1.0074825567266643 1101 16-17 1.0188684602700135 1101 18-19 1.008449928513862 1101 20-21 0.9720678678656682 1101 22-23 0.9679225090407328 1101 24-25 1.0310468368218508 1101 26-27 1.0176067048395439 1101 28-29 1.046077014947464 1101 30-31 1.1611305323259984 1101 32-33 1.052585845570988 1101 34-35 0.9939906103148601 1101 36-37 0.9871511157845063 1101 38-39 1.033218309231792 1101 40-41 1.0846827626800177 1101 42-43 1.0413557315529474 1101 44-45 0.9857009944163053 1101 46-47 1.0202179311546828 1101 48-49 0.9945765642940998 1101 50-51 0.9176034838717086 1101 52-53 0.8980525088363507 1101 54-55 0.9017073624565697 1101 56-57 0.9102044659786515 1101 58-59 0.9546686212937345 1101 60-61 0.917206523024511 1101 62-63 0.9557529066636192 1101 64-65 0.8252419145190544 1101 66-67 0.881200242741933 1101 68-69 0.7550241117754553 1101 70-71 0.8227457649822725 1101 72-73 0.8660112958139514 1101 74-75 0.9184882458112149 1101 76-77 0.9017759382705357 1101 78-79 0.9006906590366306 1101 80-81 0.859764725495566 1101 82-83 0.9166401404316282 1101 84-85 0.9177194911706898 1101 86-87 0.9805064073743566 1101 88-89 0.93311386195521 1101 90-91 0.9407195138016 1101 92-93 1.0076576197992502 1101 94-95 1.0403152906458324 1101 96-97 1.038948388999696 1101 98-99 0.9633771065609125 1101 100-101 1.0005748284821472 1102 1 1.5299030995733247 1102 2 0.9095809296818338 1102 3 0.8379427538547084 1102 4 0.7529468795439769 1102 5 0.7493626217894516 1102 6 0.7625914386257762 1102 7 0.7405457320073481 1102 8 0.7283895737658241 1102 9 0.8203601208747102 1102 10-11 0.8663869214625706 1102 12-13 0.8639611152431073 1102 14-15 1.0107613411195615 1102 16-17 0.9931636257706984 1102 18-19 1.0166955032244758 1102 20-21 0.9370545138859754 1102 22-23 0.943649934336733 1102 24-25 0.974207413073664 1102 26-27 1.0109800803024243 1102 28-29 1.0150581899338036 1102 30-31 1.1618347809091887 1102 32-33 1.038042189246518 1102 34-35 1.0100399272576226 1102 36-37 0.9985565912603604 1102 38-39 1.0007632172551055 1102 40-41 1.100310778437155 1102 42-43 0.9606813943829806 1102 44-45 0.9714441983342255 1102 46-47 1.002689899619817 1102 48-49 0.9952431609448595 1102 50-51 0.9904222305059633 1102 52-53 0.9596738039285171 1102 54-55 0.9695296415293839 1102 56-57 0.9383537168353797 1102 58-59 1.0215157743460352 1102 60-61 0.9624463034980906 1102 62-63 1.0250422719237946 1102 64-65 0.9051111227104514 1102 66-67 0.9940437299986442 1102 68-69 0.9064253957289949 1102 70-71 0.9127363851313746 1102 72-73 0.9335030680263579 1102 74-75 0.8882121971685173 1102 76-77 0.7938968298789497 1102 78-79 0.9130430885684753 1102 80-81 0.9768077128557202 1102 82-83 0.9613030053623639 1102 84-85 0.9717096678612798 1102 86-87 0.9377882359128549 1102 88-89 0.9158801463164821 1102 90-91 0.938335253676108 1102 92-93 0.9438811611282425 1102 94-95 0.9412007543712058 1102 96-97 0.9625074623049095 1102 98-99 1.001887636869558 1102 100-101 1.0192651915637683 1103 1 1.4323569316169902 1103 2 0.8479004245069852 1103 3 0.7544789822787514 1103 4 0.6657795188309876 1103 5 0.6124209471555702 1103 6 0.6283116865121983 1103 7 0.6205991204994774 1103 8 0.6308199199759272 1103 9 0.7443213925833874 1103 10-11 0.7776971629851275 1103 12-13 0.8058552268301824 1103 14-15 0.9561597028356843 1103 16-17 1.005055291156424 1103 18-19 0.9363536954155407 1103 20-21 0.9528653552798616 1103 22-23 0.9591257930526993 1103 24-25 0.9129302765909557 1103 26-27 0.9056528914263353 1103 28-29 0.8905845941378132 1103 30-31 0.912210290629659 1103 32-33 0.9247658095987816 1103 34-35 0.8967982965582308 1103 36-37 0.8987269677021601 1103 38-39 0.8997882768671914 1103 40-41 1.002561677201662 1103 42-43 0.9323077844028305 1103 44-45 0.9322083389069746 1103 46-47 0.9084576891242193 1103 48-49 0.900074785352281 1103 50-51 0.9136405365573026 1103 52-53 0.9253122704877939 1103 54-55 0.9056133603898715 1103 56-57 0.9513733487914422 1103 58-59 0.8960582419495253 1103 60-61 0.8857410852949741 1103 62-63 0.9449597948487991 1103 64-65 0.8984497948969974 1103 66-67 0.9583320422192543 1103 68-69 0.8925437153533906 1103 70-71 0.9216768393446841 1103 72-73 0.8929465598366626 1103 74-75 0.9060240186006716 1103 76-77 0.8245875325268379 1103 78-79 0.8721219453381686 1103 80-81 0.8526114925427564 1103 82-83 0.8741323978726818 1103 84-85 0.8526861958995369 1103 86-87 0.8427025085862248 1103 88-89 0.8138300979612261 1103 90-91 0.8040765159914116 1103 92-93 0.7649276714385316 1103 94-95 0.7703015056294973 1103 96-97 0.842340040524757 1103 98-99 0.8694406550004778 1103 100-101 0.9190314084639404 1104 1 1.4145396334967764 1104 2 0.7864218320573109 1104 3 0.7075299596154814 1104 4 0.6307173800878374 1104 5 0.6314661873692344 1104 6 0.6046299405558884 1104 7 0.5861377580052363 1104 8 0.6020950405129142 1104 9 0.7199277237339956 1104 10-11 0.6928631090491351 1104 12-13 0.753761985365081 1104 14-15 0.8529078144836433 1104 16-17 0.8571993149838733 1104 18-19 0.798910771125037 1104 20-21 0.788777172139902 1104 22-23 0.7931334519449038 1104 24-25 0.8409093597458508 1104 26-27 0.8409989975927417 1104 28-29 0.8388760253263996 1104 30-31 0.9079080516687696 1104 32-33 0.842935816097679 1104 34-35 0.8628950458465354 1104 36-37 0.8925932393817604 1104 38-39 0.895589146479999 1104 40-41 0.9979874880104731 1104 42-43 0.898978392781224 1104 44-45 0.8889399850084985 1104 46-47 0.9287420782167501 1104 48-49 0.8172688967504413 1104 50-51 0.8863307723535385 1104 52-53 0.9000820315835583 1104 54-55 0.8794203749554086 1104 56-57 0.9038831842939175 1104 58-59 0.9320614718751372 1104 60-61 0.8994757277264753 1104 62-63 0.9184538105288027 1104 64-65 0.8956241334948345 1104 66-67 0.9320058075317874 1104 68-69 0.819777884331522 1104 70-71 0.7903669482970059 1104 72-73 0.8220070817011909 1104 74-75 0.7593520997973222 1104 76-77 0.696030894956877 1104 78-79 0.8159009215183168 1104 80-81 0.8263043764201079 1104 82-83 0.8098209106390613 1104 84-85 0.7959121531640818 1104 86-87 0.8323638943409009 1104 88-89 0.8392060305565927 1104 90-91 0.8532015984809362 1104 92-93 0.7909493823257598 1104 94-95 0.8240436257326493 1104 96-97 0.798138824857201 1104 98-99 0.8119335434776893 1104 100-101 0.8613706290399357 1105 1 1.2856703425559601 1105 2 0.6838148659924883 1105 3 0.619517094515178 1105 4 0.5047317213118205 1105 5 0.48262263030127883 1105 6 0.468142160877008 1105 7 0.46750128689889436 1105 8 0.44100448773711776 1105 9 0.4559131658967459 1105 10-11 0.4949000657449929 1105 12-13 0.5263059095700768 1105 14-15 0.6345002947825549 1105 16-17 0.661349412274987 1105 18-19 0.6111810565375251 1105 20-21 0.643221195906925 1105 22-23 0.6551624833425578 1105 24-25 0.6701894527994625 1105 26-27 0.6304278345094403 1105 28-29 0.6098455576923243 1105 30-31 0.6875826031689485 1105 32-33 0.6826329084607607 1105 34-35 0.5884150402313253 1105 36-37 0.6360859488542658 1105 38-39 0.6326706689253925 1105 40-41 0.7479601995432361 1105 42-43 0.6360813036158035 1105 44-45 0.5954478641565544 1105 46-47 0.6891233539312154 1105 48-49 0.6688187062655402 1105 50-51 0.6587811583340013 1105 52-53 0.6392205671330018 1105 54-55 0.5754055640756164 1105 56-57 0.6170997232175068 1105 58-59 0.6272798890475855 1105 60-61 0.6036019556023362 1105 62-63 0.6100681828433068 1105 64-65 0.5775035010715186 1105 66-67 0.5778193257304522 1105 68-69 0.5520053865143808 1105 70-71 0.579334666701051 1105 72-73 0.6017415946774953 1105 74-75 0.626021284032138 1105 76-77 0.49242020440476253 1105 78-79 0.6153535869547362 1105 80-81 0.6206433295109726 1105 82-83 0.6182225214198098 1105 84-85 0.6072447889183294 1105 86-87 0.6062793224981426 1105 88-89 0.6168108493800766 1105 90-91 0.643374731265709 1105 92-93 0.660899731555503 1105 94-95 0.7016078499258 1105 96-97 0.6971804623869282 1105 98-99 0.8088923240884931 1105 100-101 0.7755380090833697 1106 1 1.1844719480700832 1106 2 0.5743473818004716 1106 3 0.5628992196861624 1106 4 0.5223513372820392 1106 5 0.3773942441094249 1106 6 0.2964165249867605 1106 7 0.37009730565922183 1106 8 0.3765065848707039 1106 9 0.41034941993596874 1106 10-11 0.4374995081615083 1106 12-13 0.47738895569747086 1106 14-15 0.5381867717479238 1106 16-17 0.5909015442447298 1106 18-19 0.5596809747486375 1106 20-21 0.5492118872714329 1106 22-23 0.5439593029877869 1106 24-25 0.4416405739415694 1106 26-27 0.4659361146604297 1106 28-29 0.43559430342657635 1106 30-31 0.5209134759360481 1106 32-33 0.5048084286682268 1106 34-35 0.42893242307427926 1106 36-37 0.469891884038141 1106 38-39 0.4878985466560053 1106 40-41 0.586815764998029 1106 42-43 0.513605851648208 1106 44-45 0.4777283945083539 1106 46-47 0.5015349946310153 1106 48-49 0.5132929634444778 1106 50-51 0.5383047468343918 1106 52-53 0.5539604308706458 1106 54-55 0.5493911854456499 1106 56-57 0.585682893061886 1106 58-59 0.6377999398832017 1106 60-61 0.6278891853673514 1106 62-63 0.6378580901034283 1106 64-65 0.6357183496772194 1106 66-67 0.689744158687553 1106 68-69 0.5758047947036005 1106 70-71 0.5832896951296505 1106 72-73 0.5552322678532455 1106 74-75 0.577152324395275 1106 76-77 0.40894111518607446 1106 78-79 0.5320660402342945 1106 80-81 0.5352778001750416 1106 82-83 0.5868377314021735 1106 84-85 0.570247930688744 1106 86-87 0.532068401695021 1106 88-89 0.573149922424701 1106 90-91 0.5947886640160931 1106 92-93 0.6016198729080351 1106 94-95 0.6178208172712552 1106 96-97 0.6466617526274838 1106 98-99 0.6549222435337647 1106 100-101 0.5982658449914418 1107 1 1.208392049508305 1107 2 0.6158762344384208 1107 3 0.5398430723882193 1107 4 0.47149976246160463 1107 5 0.4220790915888202 1107 6 0.403899089685261 1107 7 0.3306319498179633 1107 8 0.40643540289124047 1107 9 0.4465130118949361 1107 10-11 0.4475882216019613 1107 12-13 0.4697407894686094 1107 14-15 0.5131665021698169 1107 16-17 0.5282296437822964 1107 18-19 0.5254284886535601 1107 20-21 0.48930520260707766 1107 22-23 0.4829540844091724 1107 24-25 0.43404006493734926 1107 26-27 0.4267185419059061 1107 28-29 0.4683371859409178 1107 30-31 0.4681006441939104 1107 32-33 0.455864008107163 1107 34-35 0.44015218091553976 1107 36-37 0.43833376411087954 1107 38-39 0.43389396133780167 1107 40-41 0.48710301961164504 1107 42-43 0.49709435304566085 1107 44-45 0.5100174171938363 1107 46-47 0.4435886287667685 1107 48-49 0.48686350572052817 1107 50-51 0.40693416866528054 1107 52-53 0.3858573810543362 1107 54-55 0.34971082346637417 1107 56-57 0.41512193390435215 1107 58-59 0.46466446424600605 1107 60-61 0.40155198085380306 1107 62-63 0.4723079694475558 1107 64-65 0.4686060428752299 1107 66-67 0.4669764080994909 1107 68-69 0.4934195283508842 1107 70-71 0.46606082408865035 1107 72-73 0.44796411371910594 1107 74-75 0.45681398902025094 1107 76-77 0.3147000631663168 1107 78-79 0.40863433024146545 1107 80-81 0.4332077964244938 1107 82-83 0.472628545891812 1107 84-85 0.45222639627342787 1107 86-87 0.4742121319280912 1107 88-89 0.4717779167566647 1107 90-91 0.42460135893331596 1107 92-93 0.4255711525220853 1107 94-95 0.42128541121855534 1107 96-97 0.44386963402741486 1107 98-99 0.42214322998246345 1107 100-101 0.404340256796484 1108 1 1.089439152653977 1108 2 0.5289233522107217 1108 3 0.43828304067964297 1108 4 0.41754382622134045 1108 5 0.32923295586000734 1108 6 0.31233311063028424 1108 7 0.2908794287058143 1108 8 0.29594961016727694 1108 9 0.34433083591707003 1108 10-11 0.32496788537370946 1108 12-13 0.33653097898473305 1108 14-15 0.34601131285763387 1108 16-17 0.3322696961431788 1108 18-19 0.3171321643758702 1108 20-21 0.3074428280384538 1108 22-23 0.293958937711281 1108 24-25 0.3691655671501408 1108 26-27 0.3322222613918271 1108 28-29 0.34538987131041665 1108 30-31 0.4373456605296866 1108 32-33 0.4239177235830027 1108 34-35 0.362503928404486 1108 36-37 0.3196837717730361 1108 38-39 0.37070132754217155 1108 40-41 0.49168753675587595 1108 42-43 0.41566415232823317 1108 44-45 0.4077529245455338 1108 46-47 0.23718067628396966 1108 48-49 0.3895177645587964 1108 50-51 0.3585584077937156 1108 52-53 0.36541703598950903 1108 54-55 0.3900094586825702 1108 56-57 0.35877142765929193 1108 58-59 0.42132203789758194 1108 60-61 0.3993016437910981 1108 62-63 0.421341993449488 1108 64-65 0.3660320375265371 1108 66-67 0.36195264131936966 1108 68-69 0.21922576063962396 1108 70-71 0.2867875844791712 1108 72-73 0.3590018293153925 1108 74-75 0.3708818167525898 1108 76-77 0.2270478674324039 1108 78-79 0.34492697355817725 1108 80-81 0.34600619896865936 1108 82-83 0.3835873488365742 1108 84-85 0.3379887026614625 1108 86-87 0.35293539977492827 1108 88-89 0.3338343674885955 1108 90-91 0.34169683725177435 1108 92-93 0.31975640221103063 1108 94-95 0.35313032008532375 1108 96-97 0.3638043207747508 1108 98-99 0.41852815864444537 1108 100-101 0.308203445713378 1109 1 1.0375320793353602 1109 2 0.45165576726244083 1109 3 0.36612014909813695 1109 4 0.3638291730288259 1109 5 0.28100733422802904 1109 6 0.2600292470266723 1109 7 0.2615721380758913 1109 8 0.2823686976102522 1109 9 0.24906101697494165 1109 10-11 0.27538814752356444 1109 12-13 0.2592631025426684 1109 14-15 0.22719506715982618 1109 16-17 0.19907091089039852 1109 18-19 0.217113377346827 1109 20-21 0.2188407659352194 1109 22-23 0.1698529044529664 1109 24-25 0.19268154763410905 1109 26-27 0.18420226551115348 1109 28-29 0.16552514755117187 1109 30-31 0.2667367911698477 1109 32-33 0.23260416951048057 1109 34-35 0.14462910456432354 1109 36-37 0.16668618817366365 1109 38-39 0.20586950541367344 1109 40-41 0.32693716478836166 1109 42-43 0.17865265828039867 1109 44-45 0.2245957984436444 1109 46-47 0.22736791064544803 1109 48-49 0.20522897270617335 1109 50-51 0.21418139895900623 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-3.8235457394671393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1017433.0 3 341994.0 4 176261.0 5 159075.0 6 332168.0 7 313383.0 8 263543.0 9 398808.0 10 555572.0 11 472004.0 12 333547.0 13 250213.0 14 220646.0 15 210884.0 16 205299.0 17 204533.0 18 205498.0 19 208871.0 20 218224.0 21 234561.0 22 262354.0 23 301994.0 24 350266.0 25 406720.0 26 482535.0 27 603890.0 28 623079.0 29 622975.0 30 733678.0 31 893195.0 32 1099200.0 33 1403611.0 34 1875487.0 35 2671218.0 36 4133928.0 37 8112170.0 38 1.5876056E7 39 4518467.0 40 10691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.842657791334368 24.633949877805826 10.35076580709897 40.17262652376083 2 14.534619930389617 14.95229705067361 59.4736254646943 11.039457554242471 3 13.014998848065796 27.684426284232178 36.1116997431983 23.188875124503728 4 18.514806646675318 31.622117241404453 30.03632675377319 19.82674935814704 5 17.564162499272435 32.06055216588317 33.81250835420646 16.562776980637935 6 14.200349418088965 41.360891360583985 30.44400679632449 13.99475242500256 7 11.043188677757046 23.240006645069606 49.81333237455699 15.903472302616361 8 11.448689340657818 22.7161627000619 47.07761315408766 18.757534805192623 9 13.27057313909809 26.54695387658071 37.509665503025666 22.672807481295532 10-11 20.49250620407467 33.93977630384162 28.495678574754535 17.07203891732918 12-13 17.674125053064472 26.430684314847298 37.53849087056779 18.35669976152044 14-15 17.68880332298597 28.9504702705565 36.4932388600791 16.867487546378428 16-17 20.284759686740152 27.69664150191451 35.829440689160656 16.189158122184683 18-19 19.52358432846793 30.11943567850373 35.85565589421316 14.501324098815186 20-21 20.88677047656437 28.9349251654209 33.49466006667305 16.683644291341682 22-23 20.53909110415958 30.784509475169976 32.10422079351681 16.572178627153637 24-25 16.672269468728324 30.610855448927182 33.84133124256215 18.87554383978235 26-27 16.560539883404584 29.4645904598059 34.38270620504936 19.592163451740152 28-29 15.767272161187229 33.26514578345004 32.26874157405155 18.698840481311176 30-31 16.26537298988312 31.330303740405625 33.38595371989836 19.018369549812896 32-33 17.271559591024566 31.25314344598519 30.88905549328942 20.586241469700823 34-35 18.449904464477186 31.13842992908155 31.546984636043362 18.8646809703979 36-37 19.62423867985885 30.72578827105627 31.878416188884167 17.771556860200715 38-39 19.641157436125255 30.599719246397072 32.25382584147326 17.50529747600442 40-41 19.56152598391228 30.3735494214667 33.490550391083005 16.574374203538014 42-43 20.0267336433106 30.316961315484743 31.870095076492788 17.786209964711873 44-45 19.864236565793448 30.496825873902733 31.46862719422627 18.17031036607755 46-47 19.269712037267812 29.629233520227398 33.31240928762577 17.788645154879017 48-49 17.58654989457699 31.80608479022527 32.199419128722795 18.40794618647495 50-51 16.443828887283264 30.8804432824613 33.50637409195012 19.169353738305322 52-53 17.94253681304001 30.7181009466932 31.950350754543976 19.389011485722815 54-55 17.2379359886593 31.659145845813626 31.169148594388734 19.933769571138342 56-57 17.9562624758112 30.822978438948272 32.817232687660855 18.403526397579665 58-59 19.009703527597676 29.63208474625648 32.37680404203886 18.981407684106976 60-61 19.75021884753434 31.613607281344418 29.774764134900156 18.861409736221088 62-63 19.49865170053577 30.758846001960688 32.569182610689275 17.173319686814263 64-65 21.022277991898353 29.308524556754755 31.647766783046265 18.021430668300624 66-67 18.99648080111876 34.969774238644014 27.922003988116206 18.11174097212102 68-69 19.263393349523227 31.77561360176457 32.28434491234663 16.676648136365575 70-71 19.688779064238577 31.49715181989563 30.373575511560396 18.440493604305395 72-73 17.20397502719588 35.63618068013403 29.061173148247487 18.0986711444226 74-75 17.35731659468262 30.744400283839056 33.108088870255415 18.79019425122291 76-77 18.112064015789624 32.650437162155455 30.7327727981874 18.50472602386752 78-79 17.60124004788292 33.46451151411764 30.43330480040244 18.500943637596997 80-81 18.506548447743487 31.15979933246843 31.650985919081943 18.682666300706142 82-83 19.743935340191186 31.637886844593677 30.128768936747406 18.489408878467728 84-85 19.119630858253586 31.756992081603446 30.579233193512824 18.544143866630144 86-87 19.628411537138195 30.817110612266973 30.76898362423962 18.78549422635521 88-89 19.22397584438747 31.59738566042003 30.614269388503175 18.56436910668932 90-91 18.762794132598774 31.704153750968345 31.745341014889977 17.787711101542904 92-93 19.488936726657645 31.692554511669307 30.38227025950005 18.436238502173 94-95 19.16437765322342 31.576803720073123 30.878927347798374 18.379891278905088 96-97 18.067316128847462 31.970712148108287 30.11735803243753 19.844613690606717 98-99 18.255932333347832 32.119138749143495 31.47980816651339 18.145120750995282 100-101 18.32373718587608 32.82064927867917 30.273415258651927 18.582198276792827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22134.0 1 22302.5 2 18471.0 3 16898.5 4 21487.5 5 27973.5 6 34559.0 7 41538.5 8 50753.0 9 58390.5 10 64656.0 11 72495.0 12 82184.0 13 90775.5 14 100594.0 15 111447.0 16 121072.0 17 132822.5 18 149400.0 19 163753.0 20 182772.0 21 213862.5 22 252981.5 23 305238.5 24 390871.5 25 468837.5 26 534214.5 27 648180.5 28 979669.0 29 1247562.5 30 1564497.5 31 1918895.0 32 2763331.5 33 3215096.0 34 2632680.0 35 2796054.0 36 3070142.0 37 3210397.5 38 3525472.5 39 2971763.0 40 2415557.0 41 2142366.0 42 1630584.5 43 1338141.5 44 1119516.0 45 964763.5 46 879619.0 47 803493.5 48 756762.0 49 728981.0 50 638797.5 51 523770.0 52 465941.5 53 431194.0 54 392198.0 55 337193.5 56 288553.0 57 242310.0 58 199868.0 59 170132.0 60 137581.5 61 101197.5 62 72234.0 63 52162.0 64 36525.5 65 26145.5 66 19481.5 67 14940.5 68 13094.5 69 10214.0 70 7984.0 71 8359.5 72 7875.0 73 9523.0 74 9613.0 75 5534.5 76 3516.0 77 3678.5 78 3332.5 79 1772.0 80 1137.0 81 863.0 82 597.0 83 416.5 84 358.0 85 296.0 86 254.5 87 176.5 88 85.5 89 66.5 90 52.0 91 26.5 92 13.5 93 10.5 94 6.5 95 4.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05190040525275685 2 0.007093009904036585 3 0.006545294657256074 4 0.004233585466218045 5 0.03895600328169145 6 0.12471144811213762 7 0.14046069791280144 8 0.1496198222708855 9 0.15210695627405965 10-11 0.16306905786798703 12-13 0.16929176578737057 14-15 0.1723197929612977 16-17 0.18214260785863007 18-19 0.18011742586074767 20-21 0.18318541090855026 22-23 0.15524413666442702 24-25 0.17556807573268463 26-27 0.18179858031038537 28-29 0.18378965192813015 30-31 0.1977281278346335 32-33 0.20707339741003974 34-35 0.20468664538269907 36-37 0.19820664773884922 38-39 0.22835340170443919 40-41 0.25273745059135805 42-43 0.26420341122903185 44-45 0.29749904057246496 46-47 0.28143987360369405 48-49 0.27595687364215105 50-51 0.2705859506439172 52-53 0.26658334118034505 54-55 0.26804813836168156 56-57 0.28366971788240186 58-59 0.27490919768078265 60-61 0.2828764078986308 62-63 0.2915833260743196 64-65 0.38788082753185615 66-67 0.18642492243933034 68-69 0.3266936276410717 70-71 0.006754829849529757 72-73 0.004177059693418632 74-75 0.015711241091367657 76-77 0.0344690264201657 78-79 0.017376802224371025 80-81 0.024412311773318554 82-83 0.028843737444334543 84-85 0.023547857282403405 86-87 0.020593898362489295 88-89 0.03068667255405331 90-91 0.022368612711932908 92-93 0.022334502331795333 94-95 0.03245748857433834 96-97 0.03479746065177607 98-99 0.019012151306395397 100-101 0.030511247741917196 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.1304031E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.29633097203235 #Duplication Level Percentage of deduplicated Percentage of total 1 78.38503607339585 11.99003455033358 2 8.131218495121608 2.4875561861458184 3 3.1990352614360793 1.4680050645038492 4 1.8533210817960826 1.1339605065839167 5 1.2374953273033729 0.9464569051387947 6 0.8652413090534947 0.7941010460374073 7 0.6465728324584676 0.6923134429966399 8 0.5220724824021659 0.6388634785771252 9 0.42544921271187014 0.5857030772488223 >10 3.598907984825017 11.472839644994442 >50 0.5498349055615831 5.913897067002 >100 0.48401365850574807 14.866085224601955 >500 0.05483912122344467 5.773055279407825 >1k 0.03827941443892036 11.626256981186458 >5k 0.004116407267803002 4.374591252882293 >10k+ 0.004566432498959007 25.236280292359087 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG 513121 1.000157278089903 TruSeq Adapter, Index 10 (100% over 49bp) ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 323410 0.6303793165882035 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 293093 0.5712864940378661 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC 234782 0.45762875825488253 TruSeq Adapter, Index 10 (100% over 50bp) GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 197883 0.38570653444365804 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 190176 0.3706843230310694 No Hit ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA 171572 0.33442206519795686 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 157227 0.3064612993080407 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 154508 0.301161520816951 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 144274 0.28121377051249635 No Hit ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA 141328 0.2754715316619078 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 138761 0.27046802618687016 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 131499 0.2563131930120656 No Hit CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT 130140 0.25366427834881045 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 126076 0.24574287349857557 No Hit ATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCGTATTA 120481 0.2348372976774476 No Hit ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA 115653 0.22542673108863512 No Hit GTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGT 114989 0.22413248580798653 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 109677 0.21377852356279764 No Hit CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT 108774 0.21201842794769868 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 105351 0.20534643759278876 No Hit ATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAAT 103451 0.20164302489213762 No Hit ATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAAT 102508 0.19980496269386708 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 101603 0.19804096874960955 No Hit GTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTA 93552 0.18234824472174516 No Hit ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT 92250 0.17981043243950948 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 86376 0.16836103970075958 No Hit ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT 85219 0.16610585628252095 No Hit CGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTC 85068 0.16581153243104815 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 83741 0.16322499103433022 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 80679 0.157256649092544 No Hit GTCGTATTAACCCTATAGTGAGTCGTATTAACCCTATAGTGAGTCGTATT 80141 0.15620799854888595 No Hit CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC 76568 0.14924363350708253 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 76112 0.14835481445892623 No Hit TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA 75829 0.14780320088298715 No Hit CTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACTCACTAT 75211 0.1465986171729859 No Hit ATTTAATACGACTCACTATAGGAGAATTTAATACGACTCACTATAGGAGA 74127 0.14448572276903543 No Hit CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT 72762 0.14182511311830448 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 72619 0.141546382583466 No Hit CGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACG 72444 0.14120527878209024 No Hit ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG 71374 0.13911967268224987 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 71112 0.13860899156247586 No Hit GTCGTATTAAATCCCTATAGTGAGTCGTATTAAATCCCTATAGTGAGTCG 70905 0.1382055144945628 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 67376 0.13132691269424815 No Hit ATTTAATACGACTCACTATAGGATTTAATACGACTCACTATAGGATTTAA 66598 0.12981046265156046 No Hit CCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGT 66355 0.12933681565879296 No Hit CTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATACGACTCA 66339 0.12930562902552434 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 64293 0.12531763829629683 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 60989 0.11887759852632243 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 60285 0.11750538666250221 No Hit ATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTTAATAC 60246 0.1174293692439099 No Hit CCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCC 59852 0.11666139839966962 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 58757 0.11452706318534699 No Hit ATACGACTCACTATAGGGTTAATACGACTCACTATAGGGTTAATACGACT 58160 0.11336341193151081 No Hit CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC 57799 0.11265976351838708 No Hit GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT 57778 0.112618831062222 No Hit GTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGT 56999 0.11110043185495504 No Hit ATTAATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAAT 56481 0.11009076460288275 No Hit ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT 55803 0.10876923101812408 No Hit GTCGTATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTA 55162 0.10751981652279916 No Hit GGAATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGG 54931 0.10706955950498315 No Hit GTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTA 54759 0.10673430319734527 No Hit CTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATAG 54401 0.10603650227795941 No Hit CTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCT 53511 0.10430174580239124 No Hit CCTATAGTGAGTCGTATTAATCCCTATAGTGAGTCGTATTAATCCCTATA 53332 0.1039528453426983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1697969697546768 0.0 0.0 0.0 0.0 2 1.172440036924194 0.0 0.0 0.0 0.0 3 1.1725725801155857 0.0 0.0 0.0 0.0 4 1.1780614275708667 0.0 0.0 1.9491645792900756E-6 0.0 5 1.1785662611969028 0.0 0.0 1.9491645792900756E-6 0.0 6 1.1790087215564016 0.0 0.0 1.9491645792900756E-6 1.9491645792900756E-6 7 1.180199661114348 0.0 0.0 1.9491645792900756E-6 1.9491645792900756E-6 8 1.1886824253634183 0.0 0.0 1.9491645792900756E-6 3.898329158580151E-6 9 1.1890410716460078 0.0 0.0 1.9491645792900756E-6 3.898329158580151E-6 10-11 1.1935309722544023 0.0 0.0 1.9491645792900756E-6 3.898329158580151E-6 12-13 1.196606753960522 0.0 0.0 3.898329158580151E-6 3.898329158580151E-6 14-15 1.1992790585987287 0.0 0.0 4.872911448225189E-6 3.898329158580151E-6 16-17 1.201261358975867 0.0 0.0 5.847493737870227E-6 3.898329158580151E-6 18-19 1.2033956941901893 0.0 0.0 8.771240606805341E-6 3.898329158580151E-6 20-21 1.20594715062448 1.9491645792900756E-6 0.0 1.2669569765385493E-5 3.898329158580151E-6 22-23 1.208394326753779 1.9491645792900756E-6 0.0 1.6567898923965645E-5 3.898329158580151E-6 24-25 1.210390271282972 1.9491645792900756E-6 0.0 1.7542481213610682E-5 3.898329158580151E-6 26-27 1.2122741388488558 2.923746868935113E-6 0.0 2.0466228082545794E-5 3.898329158580151E-6 28-29 1.2144435590256055 3.898329158580151E-6 0.0 2.144081037219083E-5 3.898329158580151E-6 30-31 1.217003786700503 3.898329158580151E-6 0.0 2.728830411006106E-5 3.898329158580151E-6 32-33 1.2197511341750125 3.898329158580151E-6 0.0 2.8262886399706098E-5 3.898329158580151E-6 34-35 1.2231465788721358 3.898329158580151E-6 0.0 3.118663326864121E-5 3.898329158580151E-6 36-37 1.2262554963761034 3.898329158580151E-6 0.0 3.60595447168664E-5 3.898329158580151E-6 38-39 1.229661661478413 3.898329158580151E-6 0.0 3.703412700651144E-5 3.898329158580151E-6 40-41 1.2334274474456013 4.872911448225189E-6 0.0 3.703412700651144E-5 3.898329158580151E-6 42-43 1.2377731098751286 5.847493737870227E-6 0.0 3.995787387544655E-5 3.898329158580151E-6 44-45 1.242540766436072 7.796658317160302E-6 0.0 4.093245616509159E-5 3.898329158580151E-6 46-47 1.2479214352572023 7.796658317160302E-6 0.0 4.288162074438166E-5 3.898329158580151E-6 48-49 1.2530925688860588 7.796658317160302E-6 0.0 4.288162074438166E-5 3.898329158580151E-6 50-51 1.2586593829245114 7.796658317160302E-6 0.0 4.677994990296181E-5 3.898329158580151E-6 52-53 1.264684250639097 7.796658317160302E-6 0.0 5.262744364083205E-5 3.898329158580151E-6 54-55 1.2710970021049612 7.796658317160302E-6 0.0 5.457660822012212E-5 3.898329158580151E-6 56-57 1.2784599713032296 7.796658317160302E-6 0.0 5.555119050976716E-5 3.898329158580151E-6 58-59 1.2858278134129462 7.796658317160302E-6 0.0 5.6525772799412196E-5 3.898329158580151E-6 60-61 1.2938447273275662 7.796658317160302E-6 0.0 5.6525772799412196E-5 3.898329158580151E-6 62-63 1.3028498676838862 7.796658317160302E-6 0.0 5.6525772799412196E-5 3.898329158580151E-6 64-65 1.31291632815363 7.796658317160302E-6 0.0 5.847493737870227E-5 3.898329158580151E-6 66-67 1.3240694478763277 7.796658317160302E-6 0.0 6.0424101957992345E-5 3.898329158580151E-6 68-69 1.3370128752651034 7.796658317160302E-6 0.0 6.0424101957992345E-5 3.898329158580151E-6 70-71 1.3504981704069219 7.796658317160302E-6 0.0 6.334784882692745E-5 3.898329158580151E-6 72-73 1.3648557166979725 7.796658317160302E-6 0.0 6.529701340621754E-5 3.898329158580151E-6 74-75 1.381344674456477 7.796658317160302E-6 0.0 6.822076027515264E-5 3.898329158580151E-6 76-77 1.399336438105614 7.796658317160302E-6 0.0 7.699200088195798E-5 3.898329158580151E-6 78-79 1.4190736786355052 7.796658317160302E-6 0.0 7.99157477508931E-5 4.872911448225189E-6 80-81 1.4419480995557639 7.796658317160302E-6 0.0 8.47886591991183E-5 5.847493737870227E-6 82-83 1.4661674440357328 7.796658317160302E-6 0.0 9.25853175162786E-5 5.847493737870227E-6 84-85 1.4947051626411187 7.796658317160302E-6 0.0 9.550906438521371E-5 5.847493737870227E-6 86-87 1.526819598249502 7.796658317160302E-6 0.0 9.745822896450378E-5 5.847493737870227E-6 88-89 1.5636178763419193 7.796658317160302E-6 0.0 9.843281125414881E-5 5.847493737870227E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGTAT 22225 0.0 62.917007 1 AGATCGG 93395 0.0 61.907204 1 CGCGTGT 42090 0.0 53.832684 1 GATCGGA 123740 0.0 47.963337 2 ATCGGAA 126750 0.0 46.80067 3 CGGAAGA 127230 0.0 46.546585 5 TCGGAAG 129825 0.0 45.633625 4 GCGTGTT 50165 0.0 45.545036 2 AGAGCAC 130145 0.0 45.232372 9 CCGTCTT 100210 0.0 42.634785 50-51 TATGCCG 102205 0.0 42.496655 46-47 TGCCGTC 100105 0.0 42.418617 48-49 GTTTTTT 464255 0.0 41.303608 1 GAAGAGC 143905 0.0 40.96871 7 GGAAGAG 146405 0.0 40.444103 6 ATCTCGT 104120 0.0 40.4199 40-41 CTCGTAT 106615 0.0 40.16785 42-43 ACTAGCT 113035 0.0 39.871586 32-33 GCTTATC 107445 0.0 39.690594 36-37 AAGAGCA 149530 0.0 39.48481 8 >>END_MODULE