FastQCFastQC Report
Fri 27 May 2016
SRR1233090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39679370
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1462570.36859708206052666Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA809470.20400273492245466No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA770720.19423695487100728No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT765960.193037339050494No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG756650.19069103163684303Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT715510.18032292347383538No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT550310.13868919793837453No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT527580.13296078037529324No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC491640.12390317689015728No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG487380.12282957113482396No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC448430.11301338705730458No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT442900.11161971573641416No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT433290.10919780228365521No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC417310.10517052060050348No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA405420.1021740012505239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT302850.059.0959661
GTTTTTT7069400.049.6376571
GCGTGTT381850.046.6693732
CGCCGTA359850.039.0364846-47
CCGTATC364400.038.74170748-49
GTCGCCG368300.037.28944-45
AGATCGG464550.036.800871
TGGTCGC377300.034.51880642-43
GATCTCG436250.033.6216434-35
TCGGTGG415150.033.38870638-39
AGAGCGT518050.032.6724329
TCTCGGT424150.032.01098336-37
TAGGCAT273600.031.5561229
CTAACGA241900.031.55226594-95
GGTGGTC417400.031.3294840-41
AAGAGCG554600.031.0838248
CGTGTTT601700.030.2500933
ACTCGTC265950.029.66959642-43
AGCGTCG487850.029.4925810-11
TCGTCTA267100.029.47160544-45
Produced by FastQC (version 0.11.5)