##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233090_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39679370
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838740282418797	34.0	31.0	34.0	30.0	34.0
2	32.218303012371415	34.0	31.0	34.0	30.0	34.0
3	32.273257387907115	34.0	31.0	34.0	30.0	34.0
4	35.572136099943116	37.0	37.0	37.0	35.0	37.0
5	35.54616489626726	37.0	37.0	37.0	35.0	37.0
6	35.61193660584833	37.0	37.0	37.0	35.0	37.0
7	35.64511974358464	37.0	37.0	37.0	35.0	37.0
8	35.65534460350555	37.0	37.0	37.0	35.0	37.0
9	37.371849074216655	39.0	38.0	39.0	35.0	39.0
10-11	37.313023417458496	39.0	38.0	39.0	35.0	39.0
12-13	37.25359499155354	39.0	38.0	39.0	35.0	39.0
14-15	38.620608202700794	41.0	39.0	41.0	34.5	41.0
16-17	38.36626757178857	40.5	39.0	41.0	34.0	41.0
18-19	37.85671875586735	40.0	38.0	41.0	33.0	41.0
20-21	37.46606357409404	40.0	37.5	41.0	32.5	41.0
22-23	37.00311750161356	40.0	37.0	41.0	31.5	41.0
24-25	36.43464712267357	40.0	37.0	41.0	28.5	41.0
26-27	36.076237891377815	40.0	37.0	41.0	26.0	41.0
28-29	35.689847394250464	40.0	37.0	41.0	21.5	41.0
30-31	35.38369666655494	40.0	37.0	41.0	16.5	41.0
32-33	35.193484359756724	40.0	37.0	41.0	14.0	41.0
34-35	35.02684522208896	40.0	37.0	41.0	9.5	41.0
36-37	34.88383488699543	40.0	36.5	41.0	9.0	41.0
38-39	34.70375315938736	40.0	36.0	41.0	8.0	41.0
40-41	34.59328217660714	40.0	36.0	41.0	7.0	41.0
42-43	34.458646319233395	40.0	35.5	41.0	3.5	41.0
44-45	34.19577538655478	40.0	35.0	41.0	2.0	41.0
46-47	34.106070875117226	40.0	35.0	41.0	2.0	41.0
48-49	34.057655313075784	40.0	35.0	41.0	2.0	41.0
50-51	33.55519703311822	39.0	34.0	40.5	2.0	41.0
52-53	33.56708629698505	39.0	35.0	40.5	2.0	41.0
54-55	33.79977335829677	40.0	35.0	41.0	2.0	41.0
56-57	33.67441893608694	40.0	35.0	41.0	2.0	41.0
58-59	33.495854609082755	39.5	35.0	41.0	2.0	41.0
60-61	33.42593753378645	39.0	34.5	41.0	2.0	41.0
62-63	33.215993184871635	39.0	34.0	41.0	2.0	41.0
64-65	32.90353435551018	39.0	34.0	41.0	2.0	41.0
66-67	32.517514869817745	38.0	34.0	40.5	2.0	41.0
68-69	32.166095757064696	37.0	33.5	40.0	2.0	41.0
70-71	31.797520676361543	37.0	33.0	39.5	2.0	41.0
72-73	31.37386234962904	36.0	32.5	39.0	2.0	41.0
74-75	31.039641871834156	36.0	32.0	39.0	2.0	41.0
76-77	30.694691170751955	35.0	32.0	38.5	2.0	40.0
78-79	30.325347151429067	35.0	31.5	37.0	2.0	39.5
80-81	29.95563548514001	35.0	31.0	37.0	2.0	39.0
82-83	29.608730645169015	35.0	31.0	36.0	2.0	39.0
84-85	29.277824925143722	35.0	31.0	36.0	2.0	37.5
86-87	28.94451773049824	35.0	30.0	36.0	2.0	37.0
88-89	28.664850991837824	35.0	30.0	35.0	2.0	37.0
90-91	28.414665631031944	35.0	30.0	35.0	2.0	36.0
92-93	28.137300201590904	35.0	29.0	35.0	2.0	36.0
94-95	27.834772389279365	34.5	29.0	35.0	2.0	36.0
96-97	27.53536076807671	34.0	29.0	35.0	2.0	36.0
98-99	27.216464777540573	34.0	28.5	35.0	2.0	35.0
100-101	25.409416732170897	32.5	22.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.22414206562751104
1101	2	0.17113143328636937
1101	3	0.21270458946749216
1101	4	0.2538143056742186
1101	5	0.23436425790554694
1101	6	0.21684090400238887
1101	7	0.20223844099287192
1101	8	0.16619043220507734
1101	9	0.20391390150719957
1101	10-11	0.16166195930013316
1101	12-13	0.1780875930785939
1101	14-15	0.17666592667124092
1101	16-17	0.21119461610591372
1101	18-19	0.14613840081071316
1101	20-21	0.2226359805727185
1101	22-23	0.2362581764585059
1101	24-25	0.21154393344505706
1101	26-27	0.11284357617964957
1101	28-29	0.19041413152212527
1101	30-31	0.14521657876389327
1101	32-33	0.07699077884166883
1101	34-35	0.008836807513056044
1101	36-37	0.026165016036770794
1101	38-39	0.11089862574329601
1101	40-41	0.1347807306066784
1101	42-43	0.12519162304011644
1101	44-45	0.06520439819870916
1101	46-47	0.05190134626572984
1101	48-49	0.07711710379462744
1101	50-51	0.07086877205726694
1101	52-53	0.05250723057843487
1101	54-55	0.10822366613141554
1101	56-57	0.05429920906220076
1101	58-59	0.0797410270443848
1101	60-61	0.20980415295527877
1101	62-63	0.18324491616599659
1101	64-65	0.21832338789512562
1101	66-67	0.2306539050035994
1101	68-69	0.24073963971800083
1101	70-71	0.21057852225241547
1101	72-73	0.23876407488958762
1101	74-75	0.17873281684070363
1101	76-77	0.17327119290123605
1101	78-79	0.17058477269635475
1101	80-81	0.18130458469642718
1101	82-83	0.20592973625310762
1101	84-85	0.12696939650289352
1101	86-87	0.17837684359789563
1101	88-89	0.11152909785086962
1101	90-91	0.09794987923426035
1101	92-93	0.09971793692065845
1101	94-95	0.06699968905280684
1101	96-97	0.08942638138104186
1101	98-99	0.09695328301237893
1101	100-101	0.3585700096509079
1102	1	0.183091700644205
1102	2	0.19964311005731616
1102	3	0.17050246814295633
1102	4	0.20586409096076608
1102	5	0.2762027344627782
1102	6	0.22364272539805086
1102	7	0.17562264181243137
1102	8	0.1628573749872544
1102	9	0.1845164795766152
1102	10-11	0.17498003338344148
1102	12-13	0.21469323441771593
1102	14-15	0.21493989829991023
1102	16-17	0.23642834862769035
1102	18-19	0.27238262387356116
1102	20-21	0.28670348465853124
1102	22-23	0.28631166097311933
1102	24-25	0.2912875606189047
1102	26-27	0.27315338033864833
1102	28-29	0.2769292349965724
1102	30-31	0.24958208562048156
1102	32-33	0.22647026070186627
1102	34-35	0.19880068000038165
1102	36-37	0.233321777747733
1102	38-39	0.3111253151712603
1102	40-41	0.25587230409801975
1102	42-43	0.21598347892213354
1102	44-45	0.2020601231257615
1102	46-47	0.09531587999594393
1102	48-49	0.1889877533345441
1102	50-51	0.20132057322884833
1102	52-53	0.17471948510398505
1102	54-55	0.2495286786241948
1102	56-57	0.18612341893800277
1102	58-59	0.13932028522604867
1102	60-61	0.22559833692588427
1102	62-63	0.25946194389500477
1102	64-65	0.2903931088904059
1102	66-67	0.366487269238128
1102	68-69	0.26229907763965343
1102	70-71	0.28375059801768643
1102	72-73	0.3305849455833929
1102	74-75	0.33043785825406147
1102	76-77	0.29293615486701086
1102	78-79	0.2889465813052219
1102	80-81	0.22299142341928402
1102	82-83	0.16984686616285316
1102	84-85	0.17074288271978943
1102	86-87	0.21945179845836904
1102	88-89	0.2634733032559353
1102	90-91	0.2921312945280512
1102	92-93	0.21912498167173666
1102	94-95	0.19251071845112122
1102	96-97	0.12555964811625486
1102	98-99	0.19209264895464528
1102	100-101	0.4889425244650454
1103	1	0.20798946741702196
1103	2	0.14434471564857176
1103	3	0.191123600085092
1103	4	0.24606235844161262
1103	5	0.21646568296000623
1103	6	0.19454371987275465
1103	7	0.22466262923835245
1103	8	0.17871920809554354
1103	9	0.2208162319370217
1103	10-11	0.16520579509398914
1103	12-13	0.20542302881376173
1103	14-15	0.22359698899132496
1103	16-17	0.2475589695474767
1103	18-19	0.25937676662754683
1103	20-21	0.2958250963397546
1103	22-23	0.30002002834790886
1103	24-25	0.26103759960190587
1103	26-27	0.18555273064757216
1103	28-29	0.15982000742668845
1103	30-31	0.1904122902950789
1103	32-33	0.18982040738769257
1103	34-35	0.18639791856070786
1103	36-37	0.15143683832086907
1103	38-39	0.2099384476105115
1103	40-41	0.2109237597956053
1103	42-43	0.1893429065081449
1103	44-45	0.07653384090944115
1103	46-47	0.13345068568364837
1103	48-49	0.13047023189871965
1103	50-51	0.05070209357931077
1103	52-53	0.08671377065213193
1103	54-55	0.1259415976936893
1103	56-57	0.1214791277770857
1103	58-59	0.14805868515328058
1103	60-61	0.1786103134234196
1103	62-63	0.21550549211818293
1103	64-65	0.2321835317019989
1103	66-67	0.24036211397839224
1103	68-69	0.2248765724886823
1103	70-71	0.22779114057726346
1103	72-73	0.30832514799399036
1103	74-75	0.28083694841590656
1103	76-77	0.2420893228832064
1103	78-79	0.2866976645933441
1103	80-81	0.2776145023077241
1103	82-83	0.2483667899540798
1103	84-85	0.2788883747208608
1103	86-87	0.32483183472042754
1103	88-89	0.3346507950238049
1103	90-91	0.31281221266185
1103	92-93	0.25264106321898794
1103	94-95	0.2076130375444336
1103	96-97	0.2813455949053285
1103	98-99	0.28404348328104234
1103	100-101	0.4885144427259185
1104	1	0.01762570855249379
1104	2	0.008215631041771587
1104	3	0.03496693805139728
1104	4	0.14693571019589768
1104	5	0.1861743529334916
1104	6	0.12945069635376427
1104	7	0.13610477614739835
1104	8	0.07262599026826422
1104	9	0.11320778672356369
1104	10-11	0.06281339191917112
1104	12-13	0.07521030969085984
1104	14-15	0.06838707502321739
1104	16-17	0.05671486536893866
1104	18-19	0.08025622352294448
1104	20-21	0.038965004295178574
1104	22-23	0.11245165709142668
1104	24-25	0.15152251452601462
1104	26-27	0.08350489737724587
1104	28-29	0.08132076265498256
1104	30-31	0.10546722140217923
1104	32-33	0.12729280425767087
1104	34-35	0.08803380273698735
1104	36-37	0.017687586392966637
1104	38-39	0.08879004349395814
1104	40-41	0.04236604650009923
1104	42-43	0.05214353607585309
1104	44-45	-0.033780319415832594
1104	46-47	0.01885636210392505
1104	48-49	-0.044148819943437445
1104	50-51	-0.036903526878397486
1104	52-53	-0.03603796157256056
1104	54-55	0.07478508618049062
1104	56-57	0.08132622705470993
1104	58-59	0.09931935167581685
1104	60-61	0.11673622445152176
1104	62-63	0.05494747008921763
1104	64-65	0.00938659155080046
1104	66-67	0.02427197534937875
1104	68-69	0.08176641359747094
1104	70-71	0.09056479110179438
1104	72-73	0.12941608064638288
1104	74-75	0.12105956564863263
1104	76-77	0.09266008035192996
1104	78-79	0.0931165429559897
1104	80-81	0.10184527042900626
1104	82-83	0.10073853076916706
1104	84-85	0.14876708478309197
1104	86-87	0.1415994339509723
1104	88-89	0.13644023969782992
1104	90-91	0.1499208787743136
1104	92-93	0.13319603341703612
1104	94-95	0.08457181408421022
1104	96-97	0.12144671583557098
1104	98-99	0.16112417403035195
1104	100-101	0.2508573383394683
1105	1	0.06373630180739909
1105	2	0.029933211787600555
1105	3	0.019876182450779822
1105	4	0.12072423233995266
1105	5	0.1548696105535896
1105	6	0.09015450047213136
1105	7	0.09605370822478676
1105	8	0.05412371382610104
1105	9	0.08515084524625394
1105	10-11	-0.003755569260015079
1105	12-13	0.01556914078283711
1105	14-15	0.08200629953925187
1105	16-17	0.06770171164658478
1105	18-19	0.074859442909883
1105	20-21	0.09140516997706527
1105	22-23	0.04646249386704682
1105	24-25	0.11076233077472608
1105	26-27	0.04471654218490784
1105	28-29	0.03600948205946963
1105	30-31	-0.005766119979632833
1105	32-33	0.03324598813428992
1105	34-35	0.004127230351691935
1105	36-37	-0.028924223107040348
1105	38-39	0.025535327858506207
1105	40-41	0.043623414630225454
1105	42-43	0.007665469707156092
1105	44-45	-0.04829835195375409
1105	46-47	-0.03908951750965883
1105	48-49	-0.03233465458658458
1105	50-51	-0.09247223916723613
1105	52-53	-0.12020893972876223
1105	54-55	-0.06784429908180556
1105	56-57	-0.013616916052093586
1105	58-59	0.007415666590098624
1105	60-61	-0.020467354907637514
1105	62-63	0.06557396161697682
1105	64-65	0.08136317626764367
1105	66-67	0.011457586842062994
1105	68-69	-0.007230620524815379
1105	70-71	0.04535467896100087
1105	72-73	0.12906503872820352
1105	74-75	0.020658727786930342
1105	76-77	0.01981689106349549
1105	78-79	0.10346240543824692
1105	80-81	0.05140517209980189
1105	82-83	0.014951338375933432
1105	84-85	0.08606432155688637
1105	86-87	0.13796343632790808
1105	88-89	0.09464773302029528
1105	90-91	0.09794461008599598
1105	92-93	0.15322034473402368
1105	94-95	0.17968445430310354
1105	96-97	0.1298984269977943
1105	98-99	0.16835442419553104
1105	100-101	0.3919810407009372
1106	1	0.08921919323620386
1106	2	0.060086269061997655
1106	3	0.09925355046066642
1106	4	0.13587239421841701
1106	5	0.14832776901775446
1106	6	0.12209938534113718
1106	7	0.07627127532696676
1106	8	0.07237745710219201
1106	9	0.042194689426558796
1106	10-11	0.017216194899837944
1106	12-13	0.054202908164960206
1106	14-15	0.00566406462956337
1106	16-17	0.019015893431934217
1106	18-19	-0.08134105195319563
1106	20-21	-0.05789024770174933
1106	22-23	0.021466457945933826
1106	24-25	0.04689652942209932
1106	26-27	-0.09563232742025463
1106	28-29	-0.026932839652594964
1106	30-31	0.00754949169982666
1106	32-33	-0.09180749676560396
1106	34-35	-0.052194312351552696
1106	36-37	-0.04245371457169256
1106	38-39	0.09778226484742447
1106	40-41	-0.04952615654420356
1106	42-43	-0.019672965056351188
1106	44-45	-0.10961070348728441
1106	46-47	-0.15073249862595617
1106	48-49	-0.029040711807049036
1106	50-51	-0.14737397370295469
1106	52-53	-0.1456037699263817
1106	54-55	0.018732309538243896
1106	56-57	-0.021466816647780718
1106	58-59	0.055666734856323785
1106	60-61	0.10916061652123688
1106	62-63	0.10633358356550815
1106	64-65	0.08087151154489192
1106	66-67	0.10881155602339021
1106	68-69	0.08995505947389404
1106	70-71	0.08131513156513392
1106	72-73	0.17887805720258143
1106	74-75	0.14841580351905748
1106	76-77	0.06245696010553203
1106	78-79	0.1593229496416022
1106	80-81	0.16050601278703525
1106	82-83	0.1285971183110206
1106	84-85	0.1274215305884958
1106	86-87	0.12604215738278413
1106	88-89	0.1439966402397843
1106	90-91	0.17815308952487996
1106	92-93	0.19456305049302358
1106	94-95	0.16473569609434335
1106	96-97	0.1415927666809118
1106	98-99	0.1597707862287301
1106	100-101	0.30073356702561327
1107	1	0.19138162825930038
1107	2	0.08191886067142917
1107	3	0.13168195804941263
1107	4	0.1661903730873746
1107	5	0.14076314920377087
1107	6	0.1550394478488144
1107	7	0.12912788096952
1107	8	0.04949652356213363
1107	9	0.07373448811236472
1107	10-11	0.03488357543171361
1107	12-13	0.09417862616292183
1107	14-15	0.08257179171703655
1107	16-17	0.15638772656524225
1107	18-19	0.1000065668208876
1107	20-21	0.06609062413787115
1107	22-23	0.11696359551621782
1107	24-25	0.13191740353494197
1107	26-27	0.07591794330843271
1107	28-29	0.014896886010149046
1107	30-31	0.04140309346283999
1107	32-33	0.07619837728417878
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2314	98-99	0.3017354971829924
2314	100-101	0.2067746764249101
2315	1	0.12062998365224686
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2315	3	-0.10087937944183523
2315	4	-0.2051077512645776
2315	5	-0.19149277709428247
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2315	30-31	-0.2792792121955969
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2315	44-45	-4.338229547684591E-4
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2316	1	-0.02838085036434279
2316	2	-0.18997855107669892
2316	3	-0.34724928115325326
2316	4	-0.3463948688564855
2316	5	-0.38678598763750216
2316	6	-0.385771558163178
2316	7	-0.287778707979669
2316	8	-0.2305478276341546
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2316	10-11	-0.293924576407818
2316	12-13	-0.3149086422809475
2316	14-15	-0.40650535416676803
2316	16-17	-0.44583814138910327
2316	18-19	-0.4018577252237705
2316	20-21	-0.4438118802263489
2316	22-23	-0.38363282413392596
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2316	30-31	-0.2257982435821262
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2316	34-35	-0.20412620837377915
2316	36-37	-0.14878330158236963
2316	38-39	-0.10034464846044955
2316	40-41	-0.08958763079309051
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2316	50-51	-0.11685266533634575
2316	52-53	-0.15346984837213284
2316	54-55	-0.2687739967563161
2316	56-57	-0.2199167060560967
2316	58-59	-0.23883368983410946
2316	60-61	-0.24084619640736094
2316	62-63	-0.209876489854949
2316	64-65	-0.2237911976651077
2316	66-67	-0.27023793094281245
2316	68-69	-0.30471035480574216
2316	70-71	-0.26606161414170515
2316	72-73	-0.1785785078806974
2316	74-75	-0.27584541995470957
2316	76-77	-0.32029335516619284
2316	78-79	-0.276407019150124
2316	80-81	-0.28425639491089427
2316	82-83	-0.30770771853599754
2316	84-85	-0.30199299283776426
2316	86-87	-0.2958865807463411
2316	88-89	-0.37268556851532963
2316	90-91	-0.3714150328273149
2316	92-93	-0.3820658837552102
2316	94-95	-0.3570516389838936
2316	96-97	-0.36019235209169764
2316	98-99	-0.372021789479394
2316	100-101	-0.6516522296739353
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	244709.0
3	62845.0
4	58418.0
5	68991.0
6	105248.0
7	193444.0
8	386185.0
9	723832.0
10	889406.0
11	648667.0
12	413215.0
13	297043.0
14	238873.0
15	199167.0
16	178667.0
17	169614.0
18	166915.0
19	169009.0
20	179665.0
21	193952.0
22	213743.0
23	242469.0
24	288378.0
25	304940.0
26	272467.0
27	302272.0
28	341069.0
29	394360.0
30	463003.0
31	559763.0
32	695054.0
33	894825.0
34	1219069.0
35	1843650.0
36	3158634.0
37	6779839.0
38	1.1752788E7
39	4348461.0
40	16721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.910357363186566	17.027228071204398	9.57345647191268	44.488958093696354
2	13.032667562794625	15.800953613028101	59.261460715877455	11.904918108299817
3	16.107604999191285	21.930893115840632	40.53111642096581	21.430385464002274
4	18.603857909092923	27.83153902316452	36.06061399100474	17.503989076737813
5	18.928618514234344	30.257848674390065	36.58824491572804	14.225287895647554
6	14.883208287193558	34.168294336576615	36.65091722522381	14.29758015100602
7	12.089259782380882	18.64279830548958	54.37240530488604	14.895536607243494
8	12.616110661026442	21.12113932714265	47.581250092704884	18.681499919126026
9	14.284979654908081	21.49937498706826	45.69676199396536	18.5188833640583
10-11	18.334205712855834	31.042159499500332	35.09338429978971	15.530250487854131
12-13	17.06601295175095	24.158792872285247	41.56353111527649	17.211663060687314
14-15	17.450117715152796	25.934073711308603	40.301674252361806	16.314134321176795
16-17	18.62171619844986	25.53502517718792	39.045630567275076	16.797628057087145
18-19	18.430496649780043	27.133809551490458	37.94580029213364	16.489893506595855
20-21	18.780843196890025	27.421573716202595	36.772104338417286	17.02547874849009
22-23	19.668756499861114	28.077691924745825	35.7689519799574	16.484599595435665
24-25	18.555715065469673	29.09783574400136	34.584848360651186	17.76160082987778
26-27	18.51416733481241	29.512152388751495	33.96116913650356	18.012511139932542
28-29	18.753327590682893	29.733574954345272	33.22501868195245	18.288078773019382
30-31	18.57823825380075	29.752145627759873	33.444861686288405	18.22475443215097
32-33	18.558401240897197	29.80815396948105	32.930087837039665	18.70335695258209
34-35	19.450833820155264	30.507911980508716	31.57489685251356	18.46635734682246
36-37	19.073380225321777	30.224429743524006	32.32789602272699	18.374294008427235
38-39	19.24332346609855	29.37379414389743	32.501150465202805	18.881731924801212
40-41	19.78876479974994	29.421803341441816	32.334515192997365	18.45491666581088
42-43	19.728305009180705	29.801948453575218	32.34262104630902	18.12712549093506
44-45	20.327078371248607	29.069316083529493	32.29092457364644	18.31268097157545
46-47	19.508001214623725	29.45534931663038	32.038025183856114	18.998624284889775
48-49	19.17521989858765	29.320926930203424	31.880092174703158	19.623760996505773
50-51	18.724768828021567	29.59517253434002	32.725493727250736	18.954564910387674
52-53	19.184339668162664	30.2653551061209	31.83362084589688	18.716684379819547
54-55	18.897852035542716	30.073283603304795	31.311209675131636	19.717654686020854
56-57	19.42547796217496	30.308305038573565	31.32034222641006	18.945874772841417
58-59	19.053470176340053	30.987975659720885	30.93589528319055	19.022658880748512
60-61	19.245457650288298	31.47363841103837	30.140054482409855	19.14084945626348
62-63	19.49617480100299	31.389165841483063	30.39191331797597	18.722746039537974
64-65	19.99032483369452	30.50917240121731	30.61001171861511	18.89049104647306
66-67	19.792169582771287	31.568331902071336	29.717204179573447	18.92229433558393
68-69	20.083766931228688	30.957290521125145	30.403937056614105	18.555005491032052
70-71	19.879479067031507	30.740328223448177	30.348556163289857	19.03163654623046
72-73	19.337856319968996	31.316503173854727	30.08402469103585	19.26161581514043
74-75	19.19250772481443	30.257794076607752	31.495293452112712	19.054404746465107
76-77	19.375569886675624	30.683459862413226	30.69256581377115	19.248404437140007
78-79	19.529375048102068	31.09844727093937	30.099601771008704	19.27257590994985
80-81	19.340774040416857	30.44918843888304	30.84349595162233	19.366541569077775
82-83	19.88705946018257	30.75955375584767	29.969747704810946	19.383639079158815
84-85	19.577711857946543	30.545593110228488	30.283480976075182	19.593214055749794
86-87	19.827440877216016	30.832665368192135	30.198840597459764	19.14105315713208
88-89	19.749991516134294	30.54145860169998	30.383734392052492	19.324815490113238
90-91	19.758135688008814	30.91048179394574	30.22333279512343	19.10804972292202
92-93	20.048672060696564	30.582098098870986	29.964707178157248	19.404522662275202
94-95	19.914791932010882	30.27948699991177	30.267571940357733	19.53814912771961
96-97	19.66986604314892	31.040325201463542	29.830795953357462	19.459012802030074
98-99	19.545327118765183	30.634669718859648	30.23580944078893	19.58419372158624
100-101	20.134347074934123	31.286639705625674	29.132522280000405	19.446490939439798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15932.0
1	23354.5
2	26546.5
3	25383.5
4	32583.0
5	41018.5
6	48705.5
7	57074.0
8	67000.0
9	76859.0
10	86535.0
11	96231.5
12	107224.5
13	117369.5
14	126357.5
15	137384.5
16	150429.5
17	165662.5
18	185768.5
19	209154.5
20	240232.5
21	282325.5
22	325465.5
23	367536.0
24	432031.5
25	504359.5
26	583699.0
27	702709.5
28	859056.5
29	962939.0
30	1079768.5
31	1232255.0
32	1383923.5
33	1487736.5
34	1518289.5
35	1653722.0
36	1794552.0
37	1907669.5
38	1971632.0
39	1760913.5
40	1573725.0
41	1473833.0
42	1325942.0
43	1245732.5
44	1163175.5
45	1065112.5
46	953772.0
47	880226.5
48	847597.5
49	828534.0
50	757157.0
51	654734.5
52	592568.0
53	538414.0
54	486112.0
55	426871.5
56	369617.5
57	313823.5
58	263508.5
59	220394.5
60	174495.0
61	136432.5
62	109599.5
63	82607.5
64	57243.0
65	41201.5
66	29919.0
67	23549.5
68	19007.5
69	15051.5
70	14170.0
71	16142.0
72	15981.0
73	20868.0
74	22778.0
75	15257.0
76	11628.5
77	13085.0
78	12377.0
79	8425.0
80	6563.0
81	4556.5
82	3021.5
83	2009.0
84	1218.5
85	688.5
86	444.0
87	286.0
88	163.0
89	96.5
90	75.0
91	55.0
92	30.5
93	21.0
94	14.0
95	6.0
96	5.0
97	7.5
98	6.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6539947584853288
2	0.3143976328253196
3	0.3093093463933525
4	0.32950371943909396
5	0.3120135223921146
6	0.10954559006355193
7	0.10039977953278996
8	0.09437145801458037
9	0.12144850082045154
10-11	0.10006081245745586
12-13	0.12206595013983337
14-15	0.11482037139198531
16-17	0.10710099479906057
18-19	0.13267221732603113
20-21	0.12469956050209466
22-23	0.1118679555648187
24-25	0.139170556387362
26-27	0.11605779023205257
28-29	0.13666799649288788
30-31	0.11881741065949383
32-33	0.12433287121242095
34-35	0.14298866136231497
36-37	0.12779940810552182
38-39	0.13085389208548423
40-41	0.1189459409259774
42-43	0.10969680214176789
44-45	0.41726468943433326
46-47	0.08611653864464078
48-49	0.08022430799682556
50-51	0.08185613834090612
52-53	0.27816595878412387
54-55	0.32110388849419735
56-57	0.2525040090102237
58-59	0.27178732928471394
60-61	0.06835415985687274
62-63	0.06571046868939703
64-65	0.0897368078172612
66-67	0.09228095103324473
68-69	0.0875215508713974
70-71	0.10465261923261382
72-73	0.14297354015449337
74-75	0.1193315317254281
76-77	0.10196356444167334
78-79	0.0935070289674458
80-81	0.09186889812010625
82-83	0.09638635895680804
84-85	0.09224944851694974
86-87	0.11577678778670125
88-89	0.11420292207260348
90-91	0.15216723451002373
92-93	0.14688237237637594
94-95	0.12311309378147889
96-97	0.10128184998904972
98-99	0.13631894861233937
100-101	0.11906817068920196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.967937E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.010765160440435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89650496991231	20.52158462751911
2	7.879636809075566	4.099107651808535
3	3.382983077485223	2.639819351106366
4	1.907258246036991	1.9843698535192678
5	1.3293097318400475	1.728818163018976
6	0.898201482765075	1.4017744700977053
7	0.6697948789609317	1.2195314111622895
8	0.5121656129488142	1.065745558533171
9	0.4228470705734555	0.9898718266319699
>10	3.3151523590811713	17.351800500473168
>50	0.4229786071461099	7.6817646433980595
>100	0.31306793542860534	16.025577285009337
>500	0.02914265216497212	5.1867785990160575
>1k	0.01836434268265474	9.110002994794032
>5k	0.001611644822474002	2.895303651265754
>10k+	9.805790755156507E-4	6.098149412646095
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	146257	0.36859708206052666	Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA	80947	0.20400273492245466	No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA	77072	0.19423695487100728	No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT	76596	0.193037339050494	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	75665	0.19069103163684303	Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71551	0.18032292347383538	No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT	55031	0.13868919793837453	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	52758	0.13296078037529324	No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	49164	0.12390317689015728	No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG	48738	0.12282957113482396	No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC	44843	0.11301338705730458	No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT	44290	0.11161971573641416	No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT	43329	0.10919780228365521	No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC	41731	0.10517052060050348	No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA	40542	0.1021740012505239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.44569256013893366	0.0	0.0	2.5202013035993264E-6	0.0
2	0.4466754386473374	2.5202013035993264E-6	0.0	2.5202013035993264E-6	0.0
3	0.4469728224011621	2.5202013035993264E-6	0.0	2.5202013035993264E-6	0.0
4	0.4564185368870524	2.5202013035993264E-6	0.0	5.040402607198653E-6	0.0
5	0.45691753674516505	2.5202013035993264E-6	0.0	5.040402607198653E-6	0.0
6	0.4576761173375484	2.5202013035993264E-6	0.0	5.040402607198653E-6	0.0
7	0.45846494034557506	2.5202013035993264E-6	0.0	7.560603910797979E-6	0.0
8	0.46402954482392234	2.5202013035993264E-6	0.0	1.0080805214397306E-5	0.0
9	0.46454114568855304	2.5202013035993264E-6	0.0	1.0080805214397306E-5	0.0
10-11	0.4679938214744841	2.5202013035993264E-6	0.0	1.1340905866196968E-5	0.0
12-13	0.4716594542705693	2.5202013035993264E-6	0.0	1.260100651799663E-5	0.0
14-15	0.4742477010093658	2.5202013035993264E-6	0.0	1.260100651799663E-5	0.0
16-17	0.4773122657945426	2.5202013035993264E-6	0.0	1.260100651799663E-5	0.0
18-19	0.4807031966485355	2.5202013035993264E-6	0.0	1.3861107169796293E-5	0.0
20-21	0.48497997826074357	5.040402607198653E-6	0.0	1.8901509776994947E-5	0.0
22-23	0.4889719771256449	5.040402607198653E-6	0.0	2.016161042879461E-5	0.0
24-25	0.49258594579500636	5.040402607198653E-6	0.0	2.2681811732393936E-5	0.0
26-27	0.4956442100769241	5.040402607198653E-6	0.0	2.7722214339592587E-5	0.0
28-29	0.49887132784618304	5.040402607198653E-6	0.0	3.0242415643191915E-5	0.0
30-31	0.5025357005416164	1.0080805214397306E-5	0.0	3.402271759859091E-5	2.5202013035993264E-6
32-33	0.5062529974644254	1.0080805214397306E-5	0.0	3.5282818250390566E-5	2.5202013035993264E-6
34-35	0.510992236015844	1.0080805214397306E-5	0.0	3.5282818250390566E-5	3.7803019553989894E-6
36-37	0.5147019723347421	1.0080805214397306E-5	0.0	3.7803019553989894E-5	5.040402607198653E-6
38-39	0.5183776859360418	1.0080805214397306E-5	0.0	3.7803019553989894E-5	5.040402607198653E-6
40-41	0.5225473589928469	1.0080805214397306E-5	0.0	3.7803019553989894E-5	5.040402607198653E-6
42-43	0.5272248526123273	1.0080805214397306E-5	0.0	4.032322085758922E-5	5.040402607198653E-6
44-45	0.5329091666525956	1.0080805214397306E-5	0.0	4.2843422161188544E-5	5.040402607198653E-6
46-47	0.5457760544081218	1.0080805214397306E-5	0.0	4.662372411658754E-5	5.040402607198653E-6
48-49	0.5517766537119919	1.0080805214397306E-5	0.0	4.78838247683872E-5	5.040402607198653E-6
50-51	0.5578755408667022	1.1340905866196968E-5	0.0	4.78838247683872E-5	5.040402607198653E-6
52-53	0.5644835086847397	1.5121207821595958E-5	0.0	4.78838247683872E-5	5.040402607198653E-6
54-55	0.5714921885100495	1.5121207821595958E-5	0.0	5.166412672378619E-5	5.040402607198653E-6
56-57	0.5786016763875031	1.5121207821595958E-5	0.0	5.4184328027385516E-5	5.040402607198653E-6
58-59	0.5868238331404959	1.7641409125195283E-5	0.0	5.544442867918518E-5	5.040402607198653E-6
60-61	0.595723924044157	1.7641409125195283E-5	0.0	5.670452933098484E-5	5.040402607198653E-6
62-63	0.6049188785003391	1.7641409125195283E-5	0.0	6.300503258998316E-5	5.040402607198653E-6
64-65	0.6148434312339133	1.7641409125195283E-5	0.0	6.552523389358249E-5	6.300503258998316E-6
66-67	0.6256865973426493	1.7641409125195283E-5	2.5202013035993264E-6	7.686613975977945E-5	7.560603910797979E-6
68-69	0.6377923843044887	1.7641409125195283E-5	2.5202013035993264E-6	8.190654236697811E-5	7.560603910797979E-6
70-71	0.6510965269861895	1.7641409125195283E-5	2.5202013035993264E-6	9.702775018857407E-5	1.0080805214397306E-5
72-73	0.6652083941856939	1.7641409125195283E-5	2.5202013035993264E-6	1.0206815279577271E-4	1.0080805214397306E-5
74-75	0.681178909846603	1.7641409125195283E-5	2.5202013035993264E-6	1.1466915931376934E-4	1.0080805214397306E-5
76-77	0.698916086621335	1.7641409125195283E-5	2.5202013035993264E-6	1.2727016583176598E-4	1.1340905866196968E-5
78-79	0.718276273035585	2.016161042879461E-5	2.5202013035993264E-6	1.335706690907643E-4	1.260100651799663E-5
80-81	0.7395946558627318	2.016161042879461E-5	2.5202013035993264E-6	1.3609087039436363E-4	1.260100651799663E-5
82-83	0.7638314317994463	2.39419123841936E-5	2.5202013035993264E-6	1.3861107169796293E-4	1.5121207821595958E-5
84-85	0.7911151311122127	2.772221433959259E-5	2.5202013035993264E-6	1.398711723497626E-4	1.8901509776994947E-5
86-87	0.8217166754411676	2.772221433959259E-5	2.5202013035993264E-6	1.436514743051616E-4	2.6462113687792926E-5
88-89	0.8566655670188312	2.772221433959259E-5	2.5202013035993264E-6	1.436514743051616E-4	2.772221433959259E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	30285	0.0	59.095966	1
GTTTTTT	706940	0.0	49.637657	1
GCGTGTT	38185	0.0	46.669373	2
CGCCGTA	35985	0.0	39.03648	46-47
CCGTATC	36440	0.0	38.741707	48-49
GTCGCCG	36830	0.0	37.289	44-45
AGATCGG	46455	0.0	36.80087	1
TGGTCGC	37730	0.0	34.518806	42-43
GATCTCG	43625	0.0	33.62164	34-35
TCGGTGG	41515	0.0	33.388706	38-39
AGAGCGT	51805	0.0	32.672432	9
TCTCGGT	42415	0.0	32.010983	36-37
TAGGCAT	27360	0.0	31.556122	9
CTAACGA	24190	0.0	31.552265	94-95
GGTGGTC	41740	0.0	31.32948	40-41
AAGAGCG	55460	0.0	31.083824	8
CGTGTTT	60170	0.0	30.250093	3
ACTCGTC	26595	0.0	29.669596	42-43
AGCGTCG	48785	0.0	29.49258	10-11
TCGTCTA	26710	0.0	29.471605	44-45
>>END_MODULE