FastQCFastQC Report
Fri 27 May 2016
SRR1233090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39679370
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1561930.3936378022130896TruSeq Adapter, Index 6 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1387300.34962752684833454TruSeq Adapter, Index 6 (100% over 50bp)
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA792270.1996679886802638No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT777110.19584736350400722No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA716510.18057494360419535No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT623730.15719251590940078No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT536280.13515335550942467No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG490060.12350498508418858No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC483910.12195506128247499No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC467660.11785973416412608No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT460840.11614095687507137No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT436180.10992614046039541No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA421390.106198762732372No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT421090.10612315669326403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT289800.061.9669151
GTTTTTT6699000.048.748771
GCGTGTT369250.048.137682
AGATCGG443550.039.4271931
CTCGTAT447350.036.47673842-43
TGCCGTC451250.036.08928348-49
ACGCCAA468300.036.06180232-33
TATGCCG460800.035.9716846-47
TCACGCC467650.035.6016630-31
CCGTCTT472000.034.71866250-51
AGTCACG501400.033.04863428-29
ATCTCGT492050.032.8338740-41
CTAACGA251050.032.12173594-95
CGTCTGA538300.031.13364216-17
GCCAATA549650.030.4325434-35
CAATATC535850.030.34985736-37
CGTGTTT596850.030.3210583
CACACGT551150.030.30924212-13
TCGTCTA273200.030.17245544-45
ACTCGTC274350.030.08127242-43
Produced by FastQC (version 0.11.5)