FastQCFastQC Report
Fri 27 May 2016
SRR1233089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33538571
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC909850.271284665050279Illumina Single End PCR Primer 1 (100% over 50bp)
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT692210.20639221629329407No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT640650.19101887197281003No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635850.1895876839833158No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT574090.17117306518515651No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA543150.1619478659362082No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA379320.11309963086978272No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA376660.11230651419227133No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA358380.1068560732656141No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC352530.10511181290341798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT215850.064.375051
GTTTTTT5883450.050.4582061
GCGTGTT302950.046.019452
CGCCGTA222100.034.23351746-47
TCCGATC38750.033.606261
CCGTATC230450.033.44510748-49
GTCGCCG222450.033.2277644-45
CCCGAAA181350.031.5640641
CTAACGA167150.030.99997394-95
TCGTACC33300.030.832582
CGTGTTT465650.030.4001123
AGATCGG348600.030.3776931
TCGTCTA180400.029.9615344-45
AAGAGCG365500.029.1372058
CCGATCT45750.028.9876792
AGAGCGT365300.028.724419
TGGTCGC244700.028.61566742-43
TCTCGGT261750.028.45401636-37
TCGGTGG268050.028.24982638-39
CCCGTCT95200.027.7588861
Produced by FastQC (version 0.11.5)