##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233089_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33538571
Sequences flagged as poor quality	0
Sequence length	101
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766319292494604	34.0	31.0	34.0	30.0	34.0
2	32.127486051805846	34.0	31.0	34.0	30.0	34.0
3	32.185223186760105	34.0	31.0	34.0	30.0	34.0
4	35.47478937012552	37.0	37.0	37.0	33.0	37.0
5	35.440036935384036	37.0	37.0	37.0	33.0	37.0
6	35.50921054448026	37.0	37.0	37.0	35.0	37.0
7	35.54327287826306	37.0	37.0	37.0	35.0	37.0
8	35.55683982480947	37.0	37.0	37.0	35.0	37.0
9	37.27321682846893	39.0	38.0	39.0	35.0	39.0
10-11	37.22437206701502	39.0	38.0	39.0	35.0	39.0
12-13	37.163796453939554	39.0	38.0	39.0	34.0	39.0
14-15	38.53976715346638	41.0	39.0	41.0	34.0	41.0
16-17	38.3053885480094	40.5	39.0	41.0	34.0	41.0
18-19	37.775390281237684	40.0	38.0	41.0	33.0	41.0
20-21	37.37854750877729	40.0	37.0	41.0	32.0	41.0
22-23	36.89112935074067	40.0	37.0	41.0	31.0	41.0
24-25	36.2870985916484	40.0	37.0	41.0	28.0	41.0
26-27	35.874509590763424	40.0	37.0	41.0	24.0	41.0
28-29	35.48450840377188	40.0	37.0	41.0	18.0	41.0
30-31	35.18249070599937	40.0	37.0	41.0	13.5	41.0
32-33	34.987488271339885	40.0	37.0	41.0	9.5	41.0
34-35	34.808324048749725	40.0	36.0	41.0	8.0	41.0
36-37	34.665813459971204	40.0	36.0	41.0	7.0	41.0
38-39	34.47910565420334	40.0	36.0	41.0	4.5	41.0
40-41	34.377791021567376	40.0	35.0	41.0	2.0	41.0
42-43	34.260474484735795	40.0	35.0	41.0	2.0	41.0
44-45	33.98713807752871	40.0	35.0	41.0	2.0	41.0
46-47	33.90412748951051	40.0	35.0	41.0	2.0	41.0
48-49	33.85354627959551	40.0	35.0	41.0	2.0	41.0
50-51	33.35576214621666	39.0	34.0	40.5	2.0	41.0
52-53	33.36308847207593	39.0	34.5	40.5	2.0	41.0
54-55	33.594037056617594	40.0	35.0	41.0	2.0	41.0
56-57	33.46603680580189	40.0	35.0	41.0	2.0	41.0
58-59	33.287789318751834	40.0	34.5	41.0	2.0	41.0
60-61	33.22987665455395	39.0	34.0	41.0	2.0	41.0
62-63	33.03121576050452	39.0	34.0	41.0	2.0	41.0
64-65	32.748533188846956	39.0	34.0	41.0	2.0	41.0
66-67	32.415524456900684	38.0	34.0	41.0	2.0	41.0
68-69	32.08132069789139	37.5	33.0	40.0	2.0	41.0
70-71	31.724470476097505	37.0	33.0	40.0	2.0	41.0
72-73	31.303690473276276	36.5	32.0	39.0	2.0	41.0
74-75	30.97240150750609	36.0	32.0	39.0	2.0	41.0
76-77	30.623650930148457	35.5	32.0	38.5	2.0	40.5
78-79	30.250626987059167	35.0	31.0	37.0	2.0	39.5
80-81	29.886296512156107	35.0	31.0	37.0	2.0	39.0
82-83	29.52650764398996	35.0	31.0	36.5	2.0	39.0
84-85	29.185165029839823	35.0	31.0	36.0	2.0	37.5
86-87	28.850855526909598	35.0	30.0	36.0	2.0	37.0
88-89	28.57300758282158	35.0	30.0	35.0	2.0	37.0
90-91	28.314814232842537	35.0	29.5	35.0	2.0	36.0
92-93	28.040088216638686	35.0	29.0	35.0	2.0	36.0
94-95	27.74820586422719	34.5	29.0	35.0	2.0	36.0
96-97	27.44370845436438	34.0	29.0	35.0	2.0	36.0
98-99	27.125197925099435	34.0	28.0	35.0	2.0	35.0
100-101	25.36598403372642	32.5	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2306589984730678
1101	2	0.19008097010756586
1101	3	0.215716113198539
1101	4	0.26207907346790194
1101	5	0.26857241157906486
1101	6	0.2327812356617116
1101	7	0.20965553556288796
1101	8	0.17402781943378898
1101	9	0.19963602145131887
1101	10-11	0.15861691420896307
1101	12-13	0.19411853802836276
1101	14-15	0.19875007465989825
1101	16-17	0.20987241911700494
1101	18-19	0.16012461944388434
1101	20-21	0.2303902293136062
1101	22-23	0.2556001112899722
1101	24-25	0.2749978745255177
1101	26-27	0.20234575546016487
1101	28-29	0.27418535531233346
1101	30-31	0.1670867523867514
1101	32-33	0.16137287058226946
1101	34-35	0.13014765326760624
1101	36-37	0.13105969858865762
1101	38-39	0.21016763691866913
1101	40-41	0.25288735186153133
1101	42-43	0.2505125426676287
1101	44-45	0.14222368502437632
1101	46-47	0.09744526516571028
1101	48-49	0.1385455654665506
1101	50-51	0.14775793993856468
1101	52-53	0.11289958848856685
1101	54-55	0.22502265651250042
1101	56-57	0.1893795004474086
1101	58-59	0.19051868756412205
1101	60-61	0.29603969586280243
1101	62-63	0.28835737973762576
1101	64-65	0.2658695134491609
1101	66-67	0.2490092132073869
1101	68-69	0.23483444796101338
1101	70-71	0.231215174875409
1101	72-73	0.291115812460248
1101	74-75	0.23671706115794677
1101	76-77	0.20476894721690186
1101	78-79	0.20562967949496525
1101	80-81	0.22534561724859614
1101	82-83	0.21112197306388225
1101	84-85	0.13752074423696214
1101	86-87	0.15480876390771314
1101	88-89	0.12256995023385997
1101	90-91	0.1105524147179402
1101	92-93	0.09663697981553199
1101	94-95	0.04730733375087581
1101	96-97	0.07000492537985181
1101	98-99	0.0803454686162155
1101	100-101	0.3696192745153226
1102	1	0.18842452285801414
1102	2	0.20499861625002325
1102	3	0.15932739587295686
1102	4	0.18898814074036352
1102	5	0.25107486733690365
1102	6	0.20677558171155397
1102	7	0.16344497072946496
1102	8	0.1540206372485926
1102	9	0.1829129070810609
1102	10-11	0.17824667754710788
1102	12-13	0.20783075865409018
1102	14-15	0.21245725042891905
1102	16-17	0.2681224094653132
1102	18-19	0.23448297763810189
1102	20-21	0.2774322436450305
1102	22-23	0.22235579459049148
1102	24-25	0.1866305671394599
1102	26-27	0.1903072818050333
1102	28-29	0.162224703452317
1102	30-31	0.1761942885169745
1102	32-33	0.11634678228430317
1102	34-35	0.1136466997380623
1102	36-37	0.17499010757327227
1102	38-39	0.2171918230781742
1102	40-41	0.17622768071875328
1102	42-43	0.1074198880419246
1102	44-45	0.09530318285091255
1102	46-47	0.056250246218773725
1102	48-49	0.12879502871042092
1102	50-51	0.11291723504027118
1102	52-53	0.10653080455828956
1102	54-55	0.1954337281192835
1102	56-57	0.12754361239508683
1102	58-59	0.0650023379288669
1102	60-61	0.13403666446374274
1102	62-63	0.13865920882004446
1102	64-65	0.15567598761526824
1102	66-67	0.1977125390263481
1102	68-69	0.15004654949941454
1102	70-71	0.1555242339941465
1102	72-73	0.1872731449400682
1102	74-75	0.19202056317422134
1102	76-77	0.15239112329705407
1102	78-79	0.12309604307012734
1102	80-81	0.07167533395044856
1102	82-83	0.020469060028577957
1102	84-85	0.05640582574059749
1102	86-87	0.05948654316598123
1102	88-89	0.0964227047248194
1102	90-91	0.13614053619221878
1102	92-93	0.11310398583149706
1102	94-95	0.03197732582833979
1102	96-97	-0.030653419548020366
1102	98-99	0.06422704977659421
1102	100-101	0.37694472590133543
1103	1	0.1681820341094813
1103	2	0.09401455309853901
1103	3	0.14755001760483566
1103	4	0.20083800980857802
1103	5	0.17794310164143212
1103	6	0.15453877598246635
1103	7	0.18515272433896257
1103	8	0.15972612776229056
1103	9	0.1607164077493053
1103	10-11	0.13241320782087485
1103	12-13	0.16044891892833846
1103	14-15	0.18288086894710887
1103	16-17	0.22691833079366575
1103	18-19	0.2642919562772974
1103	20-21	0.23261691314802135
1103	22-23	0.23393879272138918
1103	24-25	0.25467763257466913
1103	26-27	0.19769937179474795
1103	28-29	0.21007508654620466
1103	30-31	0.20566425652918952
1103	32-33	0.2053565719994026
1103	34-35	0.18213316637550037
1103	36-37	0.1918328750278704
1103	38-39	0.2700788043030542
1103	40-41	0.24627513032280035
1103	42-43	0.24855755822650138
1103	44-45	0.1324084523486988
1103	46-47	0.17837380100772293
1103	48-49	0.19666410816476088
1103	50-51	0.10721884776071278
1103	52-53	0.1322546133135134
1103	54-55	0.22463760844053837
1103	56-57	0.21115958962671044
1103	58-59	0.2365545982319901
1103	60-61	0.25455966884116776
1103	62-63	0.2483231507617205
1103	64-65	0.27664717095573366
1103	66-67	0.2885273874757459
1103	68-69	0.2648642794311016
1103	70-71	0.26313977066319794
1103	72-73	0.3298512861873668
1103	74-75	0.2717357845244983
1103	76-77	0.1937289750435376
1103	78-79	0.21912029000036526
1103	80-81	0.2438534891125208
1103	82-83	0.24929999084826804
1103	84-85	0.22508481433080618
1103	86-87	0.2810942681140638
1103	88-89	0.2726385488369729
1103	90-91	0.23572064052494923
1103	92-93	0.1785534012434269
1103	94-95	0.15341373049207974
1103	96-97	0.23468905302782517
1103	98-99	0.23755727929370352
1103	100-101	0.4391657078934763
1104	1	-0.0026019467884381697
1104	2	0.022563608731815066
1104	3	0.05249268490572945
1104	4	0.14495926311712282
1104	5	0.17276926372667845
1104	6	0.13102495300122285
1104	7	0.14077441176880967
1104	8	0.0591579043743522
1104	9	0.10231211876573099
1104	10-11	0.05155612271879306
1104	12-13	0.028471011043890826
1104	14-15	0.026778558864336333
1104	16-17	0.02181143273104169
1104	18-19	0.04900833641204372
1104	20-21	-0.002054079387526997
1104	22-23	0.07458595628553155
1104	24-25	0.11129840684129988
1104	26-27	0.09588130305302656
1104	28-29	0.08569968492006552
1104	30-31	0.08578717886791765
1104	32-33	0.12655590168912312
1104	34-35	0.152455111817126
1104	36-37	0.09402178336010536
1104	38-39	0.13484643210013303
1104	40-41	0.12215323174388004
1104	42-43	0.06582748598068378
1104	44-45	-0.018165000657276664
1104	46-47	0.09303212385773207
1104	48-49	0.06559671347300622
1104	50-51	0.02436401891821305
1104	52-53	0.04460451011843958
1104	54-55	0.11385885234243176
1104	56-57	0.12397174797346366
1104	58-59	0.14144364434299206
1104	60-61	0.1483724877283379
1104	62-63	0.09380002966044287
1104	64-65	0.0952833039915788
1104	66-67	0.10917618219148295
1104	68-69	0.1294085087681296
1104	70-71	0.15872615650345878
1104	72-73	0.2026786041256834
1104	74-75	0.1831263744291185
1104	76-77	0.14426361731579718
1104	78-79	0.1474374041051476
1104	80-81	0.12745082735184
1104	82-83	0.12223946204085578
1104	84-85	0.186709397212244
1104	86-87	0.182878344338544
1104	88-89	0.16737110038873837
1104	90-91	0.2026906639540833
1104	92-93	0.16351811966796959
1104	94-95	0.11208887922115451
1104	96-97	0.1430286463362549
1104	98-99	0.19805317643412224
1104	100-101	0.2572797130477831
1105	1	0.14011007644800344
1105	2	0.08056569617934173
1105	3	0.06918208516866287
1105	4	0.17396556886066605
1105	5	0.19956985330203025
1105	6	0.14232062286753688
1105	7	0.17751976125519775
1105	8	0.09835302789682032
1105	9	0.14177807270807818
1105	10-11	0.07010812283965606
1105	12-13	0.0775815351717668
1105	14-15	0.13644232811234502
1105	16-17	0.10743673520022412
1105	18-19	0.10133373384381628
1105	20-21	0.13956737408771858
1105	22-23	0.09716067157615527
1105	24-25	0.16939864771550361
1105	26-27	0.15652876726789344
1105	28-29	0.15938181161766352
1105	30-31	0.11540670136191267
1105	32-33	0.13414789927866622
1105	34-35	0.11364466471543011
1105	36-37	0.06668575070113292
1105	38-39	0.13609347509563463
1105	40-41	0.14021103263341672
1105	42-43	0.11007004410033261
1105	44-45	0.08166731366021907
1105	46-47	0.10852835143807482
1105	48-49	0.09330586023144605
1105	50-51	0.023543006587793514
1105	52-53	-7.985416612967811E-4
1105	54-55	0.03899351111838456
1105	56-57	0.07487303479756946
1105	58-59	0.12110586826178604
1105	60-61	0.07930190426993278
1105	62-63	0.1630749059897454
1105	64-65	0.18457278723430903
1105	66-67	0.10992973306130693
1105	68-69	0.06754123556830649
1105	70-71	0.14447467854919083
1105	72-73	0.24920470180648024
1105	74-75	0.1403400153920451
1105	76-77	0.1278142679886578
1105	78-79	0.20264392991827407
1105	80-81	0.20764090992499362
1105	82-83	0.1304943677092183
1105	84-85	0.19387265704581225
1105	86-87	0.2229408657262546
1105	88-89	0.2081658063198617
1105	90-91	0.22863694159432768
1105	92-93	0.25980376701264163
1105	94-95	0.2853889021293874
1105	96-97	0.21553608753436393
1105	98-99	0.2711709801024291
1105	100-101	0.4560401245968819
1106	1	0.1227280933228343
1106	2	0.13203295899477752
1106	3	0.16942687657741828
1106	4	0.20496686882978565
1106	5	0.20592149011149274
1106	6	0.17671066841529637
1106	7	0.1286695884406086
1106	8	0.14543772874799288
1106	9	0.1021903419056116
1106	10-11	0.08750216350517803
1106	12-13	0.10117773849593448
1106	14-15	0.09645903630251951
1106	16-17	0.08812020353659733
1106	18-19	0.006139596488047516
1106	20-21	-0.011194002586414342
1106	22-23	0.06394879585987923
1106	24-25	0.07280111373731302
1106	26-27	-0.08800194226699176
1106	28-29	-0.01846687767388744
1106	30-31	0.0285935325001887
1106	32-33	-0.10328938781635344
1106	34-35	-0.09886334811906039
1106	36-37	-0.08016405263693116
1106	38-39	0.043955432349498835
1106	40-41	-0.041897433666434836
1106	42-43	-0.04967782067534898
1106	44-45	-0.15288409441436102
1106	46-47	-0.15724484505904002
1106	48-49	-0.08447044524004355
1106	50-51	-0.14866915872547537
1106	52-53	-0.14951066788737677
1106	54-55	-0.03610918398087648
1106	56-57	-0.05776232113276336
1106	58-59	5.946440207296178E-4
1106	60-61	0.09173750763002175
1106	62-63	0.05736439547020922
1106	64-65	0.0200579186185621
1106	66-67	0.08697116260020721
1106	68-69	0.09296564054228185
1106	70-71	0.06325903424492552
1106	72-73	0.19523360133307932
1106	74-75	0.1872082398972985
1106	76-77	0.08942723172212297
1106	78-79	0.14209829113081085
1106	80-81	0.1019824545712531
1106	82-83	0.09035574657920975
1106	84-85	0.10427025273176227
1106	86-87	0.10029436990942386
1106	88-89	0.07929236536503836
1106	90-91	0.13677587121682322
1106	92-93	0.1354712746453295
1106	94-95	0.11282091689079721
1106	96-97	0.10549330913264399
1106	98-99	0.102431386409787
1106	100-101	0.2066181062944814
1107	1	0.19507123760789824
1107	2	0.08486929689370726
1107	3	0.12315109725437168
1107	4	0.16260542753226304
1107	5	0.17690653222484087
1107	6	0.1451369611524811
1107	7	0.12616459596240048
1107	8	0.09910543614502387
1107	9	0.05915944992610633
1107	10-11	0.03654853207773101
1107	12-13	0.08284092983675606
1107	14-15	0.057616835474860295
1107	16-17	0.10327786828688801
1107	18-19	0.046874966119169414
1107	20-21	0.03577736462433023
1107	22-23	0.05510004989150019
1107	24-25	0.10534688517323332
1107	26-27	0.05475074063132013
1107	28-29	-0.016901394262895053
1107	30-31	0.02364938037460007
1107	32-33	0.07335964153193686
1107	34-35	0.04776582470054791
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2309	5	0.1569689811502002
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2312	78-79	0.08867324029736068
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2312	82-83	-0.0053156126343942844
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2312	96-97	0.19438126695973423
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2313	1	0.3481006592599911
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2313	54-55	0.002364490700337285
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2313	100-101	-0.008904264863744515
2314	1	0.22841130595393722
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2314	4	-0.06022882206880098
2314	5	-0.08641839389536443
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2314	7	-0.06601651826380106
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2314	10-11	-0.04256912131219792
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2314	86-87	-0.0309563869185574
2314	88-89	-0.006549561652892777
2314	90-91	0.016002440302703036
2314	92-93	-8.937593761118023E-4
2314	94-95	0.011381587146875205
2314	96-97	0.04685048241325518
2314	98-99	0.05399229797324878
2314	100-101	-0.07421907587862364
2315	1	0.1475700424849613
2315	2	-0.08273566952742328
2315	3	-0.11822882006332236
2315	4	-0.19521738551299705
2315	5	-0.19548634436151957
2315	6	-0.19111099727936676
2315	7	-0.14769699533537306
2315	8	-0.13506350133537381
2315	9	-0.1308540234618505
2315	10-11	-0.1681796820575272
2315	12-13	-0.20030852208996208
2315	14-15	-0.24339865832012464
2315	16-17	-0.25754981326118553
2315	18-19	-0.24703050206122157
2315	20-21	-0.22108835814369598
2315	22-23	-0.23016977736634203
2315	24-25	-0.3272687592024255
2315	26-27	-0.2172880777792514
2315	28-29	-0.3458275534600048
2315	30-31	-0.31402410482510845
2315	32-33	-0.3115680787794801
2315	34-35	-0.26164137388320086
2315	36-37	-0.1796921682662429
2315	38-39	-0.11093640796634929
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2315	64-65	-0.23665288627344694
2315	66-67	-0.24240144201957747
2315	68-69	-0.26136323624875146
2315	70-71	-0.22912686218417022
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2315	80-81	-0.25529806602717287
2315	82-83	-0.2681282512728771
2315	84-85	-0.2587989220625211
2315	86-87	-0.2633212644428262
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2315	90-91	-0.30094227311525046
2315	92-93	-0.27684458472636564
2315	94-95	-0.26589863774673717
2315	96-97	-0.272442620562547
2315	98-99	-0.280126314777565
2315	100-101	-0.47611397451582604
2316	1	-0.03384697051081886
2316	2	-0.17975007722790082
2316	3	-0.3055424891159255
2316	4	-0.332834003018327
2316	5	-0.35594080924467164
2316	6	-0.384777970373122
2316	7	-0.2735558864273955
2316	8	-0.23721734743019596
2316	9	-0.2985798459039728
2316	10-11	-0.3201805576000396
2316	12-13	-0.3473751093618276
2316	14-15	-0.4244417432991554
2316	16-17	-0.4541237927668931
2316	18-19	-0.39573407950798156
2316	20-21	-0.41962238075893765
2316	22-23	-0.43597816937612066
2316	24-25	-0.3997339638691244
2316	26-27	-0.3271342190232147
2316	28-29	-0.2668493023657703
2316	30-31	-0.31832831517937876
2316	32-33	-0.3079004304206805
2316	34-35	-0.3342813801140494
2316	36-37	-0.27930790025355634
2316	38-39	-0.2372699988723781
2316	40-41	-0.20251864066073466
2316	42-43	-0.23024810290417008
2316	44-45	-0.13294871802686714
2316	46-47	-0.1843722201360194
2316	48-49	-0.24456067779214408
2316	50-51	-0.18273308019629297
2316	52-53	-0.22722775425898334
2316	54-55	-0.32672848514165054
2316	56-57	-0.26629295451230206
2316	58-59	-0.30319853819207054
2316	60-61	-0.30248765634249963
2316	62-63	-0.27334067984741495
2316	64-65	-0.2875731842801912
2316	66-67	-0.28385012149274047
2316	68-69	-0.32004023856306674
2316	70-71	-0.2885043752444112
2316	72-73	-0.23088727458296532
2316	74-75	-0.282206335447718
2316	76-77	-0.3491012901926105
2316	78-79	-0.32266895482018043
2316	80-81	-0.3119100905791967
2316	82-83	-0.33433058369971747
2316	84-85	-0.37771409342636986
2316	86-87	-0.3481853807817821
2316	88-89	-0.42692024599440614
2316	90-91	-0.4382597980847116
2316	92-93	-0.42430250919183266
2316	94-95	-0.391126012147442
2316	96-97	-0.3903841729201467
2316	98-99	-0.38903744414299624
2316	100-101	-0.6782424693960891
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	228842.0
3	56820.0
4	54374.0
5	63191.0
6	91667.0
7	168285.0
8	334452.0
9	631045.0
10	784833.0
11	576497.0
12	370591.0
13	274427.0
14	222792.0
15	185029.0
16	160451.0
17	149245.0
18	143547.0
19	143492.0
20	149443.0
21	161171.0
22	175106.0
23	195060.0
24	222957.0
25	242643.0
26	230729.0
27	256411.0
28	289065.0
29	331537.0
30	388258.0
31	466415.0
32	578993.0
33	743331.0
34	1005961.0
35	1506393.0
36	2569856.0
37	5538984.0
38	9881661.0
39	3951790.0
40	13227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.13416624701592	16.772200725377427	9.015295819558103	45.07833720804855
2	12.53288921292444	15.693917898306534	59.92839497649709	11.844797912271941
3	17.064111424513342	21.390100114634986	40.126073958978466	21.419714501873205
4	19.013288988339696	28.12473121432115	36.025156619311524	16.836823178027625
5	19.17843314085221	30.64449918266563	36.25173001194968	13.925337664532474
6	14.775286427744575	34.07827277329254	36.938450388060126	14.207990410902763
7	12.115949693966757	18.208552694217765	54.76005781904716	14.91543979276832
8	12.6198205832065	20.485467111989866	48.22971254102681	18.664999763776834
9	14.05761522969814	20.948113144320427	45.94351501102074	19.05075661496069
10-11	18.294098315181124	30.576120838031823	35.454149199014786	15.675631647772267
12-13	17.06564289685432	24.10433948633523	41.8576980500005	16.972319566809944
14-15	17.34647501477414	25.718261007348275	40.59131088758948	16.343953090288107
16-17	18.40330741847215	25.576538526298958	39.264732364418144	16.755421690810746
18-19	18.463243503036004	27.1331327417696	38.04376126573471	16.359862489459687
20-21	18.91721181841726	27.24278477819383	36.60271330702673	17.23729009636218
22-23	19.628608037366803	28.148117866709388	35.5299623335883	16.693311762335508
24-25	18.74815446058116	29.13240302694815	34.45314159833123	17.666300914139462
26-27	18.598985502255967	29.1391712887768	34.36531366817249	17.896529540794745
28-29	19.156074754984015	29.934255092212837	32.85139211447767	18.058278038325483
30-31	18.88965417163796	29.86192162438587	32.82945126311307	18.41897294086309
32-33	18.94466687961571	29.703213430304707	32.568431897199076	18.783687792880503
34-35	19.590291353483504	30.476507103084945	31.381479391687417	18.551722151744134
36-37	19.449339318512017	29.897145919085684	32.15934426894152	18.494170493460775
38-39	19.57995852367747	29.342726981997203	32.25817618738517	18.81913830694016
40-41	19.80955869992711	28.93937896341615	32.55823742314152	18.69282491351522
42-43	19.898533368430417	29.217146522580567	32.55052776441887	18.333792344570146
44-45	20.18836324908548	28.98432772706751	32.28213990084755	18.545169122999464
46-47	19.562065016478215	28.577498325200924	32.665080905213514	19.19535575310735
48-49	19.387125326440373	29.27529032493036	31.76481560832852	19.57276874030075
50-51	18.80326254516523	29.180840804962386	32.86896187229226	19.146934777580128
52-53	19.347475825412484	29.952031995673593	31.519430742823285	19.181061436090634
54-55	19.04966844505902	30.297626141769296	30.931917320431072	19.720788092740612
56-57	19.506725153711745	29.900968930278175	31.222179876915213	19.370126039094867
58-59	19.54429850416395	30.6066688385489	30.371005862527266	19.478026794759877
60-61	19.838834262706463	31.13172482678237	29.6675309944209	19.36190991609027
62-63	19.857720542646902	30.65506272046925	30.35131536532449	19.13590137155936
64-65	20.46335949090526	29.899385463912274	30.303535163114397	19.333719882068067
66-67	20.121588853698995	31.328713086020265	29.28793266433056	19.261765395950178
68-69	20.375253500663767	30.48499993494244	30.249390100292494	18.8903564641013
70-71	20.441338122059076	30.210353566124944	29.89028519015351	19.45802312166247
72-73	19.77699478384867	31.231936553454798	29.622537708031356	19.36853095466517
74-75	19.569182186884078	29.7382945608138	31.135599976607615	19.55692327569451
76-77	19.730703709222126	30.53221900508931	30.15257423960015	19.584503046088415
78-79	19.715963622808165	30.880647995720384	29.819493612998656	19.583894768472796
80-81	19.616012770525796	30.14857184536939	30.38446324719412	19.850952136910692
82-83	20.187028651224622	30.39308849902609	29.623149597000076	19.796733252749217
84-85	19.778606450731438	30.12164873132175	29.97086795040161	20.128876867545205
86-87	19.921203914606693	30.34002579102286	29.83087413919349	19.907896155176953
88-89	19.798271239194797	30.04989517261489	30.077880385914746	20.07395320227557
90-91	19.739529150974665	30.617345120597562	30.023055111928954	19.62007061649882
92-93	20.083968634788548	30.125578198851883	29.82566952817958	19.964783638179988
94-95	19.94151201717191	30.01857147797914	29.86704504315943	20.172871461689525
96-97	19.761439626899367	30.81616211270875	29.38521922579768	20.037179034594203
98-99	19.804071388737295	30.199960862825996	29.927281530341133	20.068686218095575
100-101	20.348899508170508	31.014833962936162	28.78539380547478	19.850872723418554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13496.0
1	20525.5
2	23653.0
3	23173.0
4	30306.0
5	38587.5
6	46870.0
7	55600.5
8	65797.5
9	75181.5
10	84004.5
11	92615.5
12	101862.5
13	111218.0
14	119018.0
15	127182.0
16	134907.5
17	143163.0
18	158469.5
19	175264.5
20	193364.0
21	217431.5
22	254941.0
23	307616.5
24	366233.5
25	436120.5
26	508192.0
27	602018.5
28	717508.5
29	790916.0
30	907264.0
31	1036753.0
32	1229860.5
33	1327138.0
34	1249095.0
35	1303831.5
36	1368635.5
37	1410811.5
38	1463092.5
39	1346603.0
40	1214513.0
41	1146269.0
42	1039263.0
43	955250.0
44	892274.5
45	846618.5
46	815383.0
47	791601.0
48	771961.5
49	767948.5
50	714279.0
51	628439.5
52	579462.5
53	543254.5
54	503026.5
55	447217.5
56	403214.5
57	364139.0
58	317028.0
59	266601.0
60	207797.5
61	156116.0
62	119239.0
63	87107.5
64	57802.5
65	39231.0
66	28059.0
67	19375.0
68	13433.5
69	9880.5
70	9540.0
71	13829.5
72	13706.0
73	19703.5
74	21134.0
75	10923.5
76	7313.5
77	8094.5
78	7406.5
79	3711.0
80	2135.0
81	1215.5
82	776.5
83	510.0
84	350.5
85	240.5
86	187.0
87	123.0
88	68.0
89	54.0
90	57.0
91	50.5
92	32.5
93	20.5
94	13.0
95	7.0
96	4.0
97	2.5
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6713315245303684
2	0.320219367724403
3	0.31455424859932163
4	0.33625761813167293
5	0.31820377797253196
6	0.11088725276935621
7	0.10149806322994502
8	0.09577032963032324
9	0.12308813037979466
10-11	0.10143544875540463
12-13	0.12400051272309723
14-15	0.1167431969597035
16-17	0.10862120511932365
18-19	0.1345868313828875
20-21	0.12685692541879617
22-23	0.11340524913837265
24-25	0.141170296134561
26-27	0.11786876668060783
28-29	0.1390145692253853
30-31	0.12052391856528413
32-33	0.12610704254513408
34-35	0.14505835683935372
36-37	0.12969395744380402
38-39	0.13274715848805843
40-41	0.12069685378068135
42-43	0.11095433970636376
44-45	0.42856328017076223
46-47	0.087444095337276
48-49	0.08134663817370155
50-51	0.08307599032767378
52-53	0.2848943683378758
54-55	0.32985007023704144
56-57	0.26060293385785577
58-59	0.2770750131244411
60-61	0.07008945014383589
62-63	0.06726285386458475
64-65	0.09224453838537128
66-67	0.09432274261178271
68-69	0.08880223310647314
70-71	0.10606742905056987
72-73	0.14448290000191122
74-75	0.12072667019712914
76-77	0.10322145210062766
78-79	0.09483558497468482
80-81	0.09366827227075357
82-83	0.09778442856137191
84-85	0.09337458056874277
86-87	0.11723218618944736
88-89	0.11549985239383037
90-91	0.1539645204323106
92-93	0.14871235867503121
94-95	0.12455957053149343
96-97	0.10278762324131221
98-99	0.1375043677323044
100-101	0.11947885316878887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3538571E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.995920881877737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75990593780634	20.734322043052238
2	7.758139962901122	4.0335998533222295
3	3.093007600917805	2.4121674264151722
4	1.815074912362629	1.8873817526584031
5	1.156489960130268	1.5032010752116194
6	0.8250749989882223	1.286915063718791
7	0.611694359116587	1.1131090714439993
8	0.4892166934925048	1.017411076649999
9	0.4079847334421632	0.9545344966418808
>10	3.2437174191839455	17.047385734056505
>50	0.44641192985989964	8.092254032482405
>100	0.33971608847492896	17.381099387317096
>500	0.03249953241002143	5.747053775611391
>1k	0.018766427327744733	9.265609510559226
>5k	0.001402660587232513	2.5089964470331414
>10k+	8.967829982881445E-4	5.014959253826078
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	90985	0.271284665050279	Illumina Single End PCR Primer 1 (100% over 50bp)
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT	69221	0.20639221629329407	No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT	64065	0.19101887197281003	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63585	0.1895876839833158	No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT	57409	0.17117306518515651	No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA	54315	0.1619478659362082	No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA	37932	0.11309963086978272	No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA	37666	0.11230651419227133	No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA	35838	0.1068560732656141	No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	35253	0.10511181290341798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.32808493838333186	0.0	0.0	0.0	0.0
2	0.33183882521410946	0.0	0.0	0.0	0.0
3	0.3320684116207575	0.0	0.0	0.0	0.0
4	0.3456974955790454	0.0	0.0	0.0	0.0
5	0.34637134659076557	0.0	0.0	0.0	0.0
6	0.3469736382030111	0.0	0.0	0.0	0.0
7	0.3480738639699348	2.9816416447796776E-6	0.0	0.0	0.0
8	0.3560348471614965	2.9816416447796776E-6	0.0	0.0	0.0
9	0.35663415713209723	2.9816416447796776E-6	0.0	0.0	0.0
10-11	0.36119606884861016	2.9816416447796776E-6	0.0	0.0	0.0
12-13	0.3658131409355515	2.9816416447796776E-6	0.0	0.0	0.0
14-15	0.3691108365946778	2.9816416447796776E-6	0.0	0.0	0.0
16-17	0.3735892623451369	2.9816416447796776E-6	0.0	0.0	0.0
18-19	0.37786493646375097	2.9816416447796776E-6	0.0	2.9816416447796776E-6	0.0
20-21	0.38419645249644063	2.9816416447796776E-6	0.0	2.9816416447796776E-6	0.0
22-23	0.389834736846719	5.963283289559355E-6	0.0	4.472462467169516E-6	0.0
24-25	0.3940880486529972	5.963283289559355E-6	0.0	7.454104111949193E-6	0.0
26-27	0.39800741659506006	5.963283289559355E-6	0.0	1.6399029046288226E-5	0.0
28-29	0.401980454086729	5.963283289559355E-6	0.0	1.7889849868678065E-5	0.0
30-31	0.4062680547719222	7.454104111949193E-6	0.0	2.236231233584758E-5	2.9816416447796776E-6
32-33	0.41058249023191834	8.944924934339032E-6	0.0	2.385313315823742E-5	2.9816416447796776E-6
34-35	0.41664864015822256	8.944924934339032E-6	0.0	2.534395398062726E-5	2.9816416447796776E-6
36-37	0.42109724949223387	8.944924934339032E-6	0.0	3.279805809257646E-5	2.9816416447796776E-6
38-39	0.42598416014802776	8.944924934339032E-6	0.0	3.279805809257646E-5	2.9816416447796776E-6
40-41	0.43127210160504453	8.944924934339032E-6	0.0	3.279805809257646E-5	2.9816416447796776E-6
42-43	0.43647804791682987	1.192656657911871E-5	0.0	3.4288878914966293E-5	2.9816416447796776E-6
44-45	0.4423682809860921	1.192656657911871E-5	0.0	3.577969973735613E-5	2.9816416447796776E-6
46-47	0.4566264913314285	1.192656657911871E-5	0.0	3.876134138213581E-5	2.9816416447796776E-6
48-49	0.4633158043614917	1.192656657911871E-5	0.0	3.876134138213581E-5	2.9816416447796776E-6
50-51	0.46979938411806516	1.192656657911871E-5	0.0	3.876134138213581E-5	2.9816416447796776E-6
52-53	0.4765498208018463	1.192656657911871E-5	0.0	3.876134138213581E-5	2.9816416447796776E-6
54-55	0.4835671144128353	1.192656657911871E-5	0.0	3.876134138213581E-5	2.9816416447796776E-6
56-57	0.4911598648612667	1.192656657911871E-5	0.0	4.472462467169516E-5	2.9816416447796776E-6
58-59	0.49940708565072733	1.192656657911871E-5	0.0	4.472462467169516E-5	2.9816416447796776E-6
60-61	0.508480221175792	1.192656657911871E-5	0.0	4.472462467169516E-5	2.9816416447796776E-6
62-63	0.5179573691437241	1.192656657911871E-5	0.0	4.472462467169516E-5	2.9816416447796776E-6
64-65	0.5276581402350148	1.192656657911871E-5	0.0	4.6215445494085E-5	2.9816416447796776E-6
66-67	0.5383652153814187	1.192656657911871E-5	0.0	4.770626631647484E-5	5.963283289559355E-6
68-69	0.5501829520405028	1.192656657911871E-5	0.0	4.770626631647484E-5	5.963283289559355E-6
70-71	0.5629995386505884	1.192656657911871E-5	0.0	4.770626631647484E-5	5.963283289559355E-6
72-73	0.576650984921212	1.192656657911871E-5	0.0	4.770626631647484E-5	7.454104111949193E-6
74-75	0.5919661872296229	1.192656657911871E-5	0.0	4.770626631647484E-5	8.944924934339032E-6
76-77	0.6087289765565742	1.192656657911871E-5	0.0	6.55961161851529E-5	8.944924934339032E-6
78-79	0.6269915316308498	1.192656657911871E-5	0.0	7.752268276427161E-5	8.944924934339032E-6
80-81	0.6472174977282127	1.192656657911871E-5	0.0	7.752268276427161E-5	8.944924934339032E-6
82-83	0.6693621502239914	1.192656657911871E-5	0.0	7.752268276427161E-5	8.944924934339032E-6
84-85	0.6951339697806445	1.192656657911871E-5	0.0	7.752268276427161E-5	8.944924934339032E-6
86-87	0.7240111691103357	1.192656657911871E-5	0.0	7.752268276427161E-5	1.0435745756728871E-5
88-89	0.7563649625978399	1.192656657911871E-5	0.0	7.752268276427161E-5	1.192656657911871E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	21585	0.0	64.37505	1
GTTTTTT	588345	0.0	50.458206	1
GCGTGTT	30295	0.0	46.01945	2
CGCCGTA	22210	0.0	34.233517	46-47
TCCGATC	3875	0.0	33.60626	1
CCGTATC	23045	0.0	33.445107	48-49
GTCGCCG	22245	0.0	33.22776	44-45
CCCGAAA	18135	0.0	31.564064	1
CTAACGA	16715	0.0	30.999973	94-95
TCGTACC	3330	0.0	30.83258	2
CGTGTTT	46565	0.0	30.400112	3
AGATCGG	34860	0.0	30.377693	1
TCGTCTA	18040	0.0	29.96153	44-45
AAGAGCG	36550	0.0	29.137205	8
CCGATCT	4575	0.0	28.987679	2
AGAGCGT	36530	0.0	28.72441	9
TGGTCGC	24470	0.0	28.615667	42-43
TCTCGGT	26175	0.0	28.454016	36-37
TCGGTGG	26805	0.0	28.249826	38-39
CCCGTCT	9520	0.0	27.758886	1
>>END_MODULE