FastQCFastQC Report
Fri 27 May 2016
SRR1233089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33538571
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG930530.27745069997168337TruSeq Adapter, Index 5 (100% over 49bp)
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT721840.21522682048677627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC669850.1997252655755667TruSeq Adapter, Index 5 (100% over 50bp)
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT615100.18340077757039797No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT589770.17584827928417104No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA560790.16720748179759956No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544710.16241300203279382No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA397660.11856796164630866No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA393450.11731269051385641No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC342660.10216893260002044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT210700.068.123051
GTTTTTT5495950.049.2801931
GCGTGTT299150.047.9243932
AGATCGG321750.033.3215981
CCCGAAA186750.032.6201931
TCGTACC32300.032.480932
TATGCCG287600.032.0180946-47
TGCCGTC283800.031.94310848-49
CTAACGA183000.031.68613694-95
CGTGTTT466450.031.2933523
CTCGTAT289950.030.82634542-43
TCGTCTA192700.030.80653244-45
CCGTCTT310350.029.54667750-51
ATCTCGT299450.029.0222340-41
CGTCTGA333150.028.44662916-17
CGTACCA37200.028.0731933
ACTCGTC216500.027.5413142-43
GCCTAAC213600.027.53304192-93
GATCGGA395250.027.4323432
CCCGTCT94900.027.3679071
Produced by FastQC (version 0.11.5)