FastQCFastQC Report
Fri 27 May 2016
SRR1233088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38719552
Sequences flagged as poor quality0
Sequence length101
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2573030.6645298995194986Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1610510.4159423125556824Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1168500.3017855165266375No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT565420.14602958216045475No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT516760.1334622879934148No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT496410.1282065453649877No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC493880.12755312871388594No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT479730.12389864428183467No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA478180.1234983297327407No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA461380.11915943655546428No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT441510.11402766230353077No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT440730.11382621369172867No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT412150.10644492994133815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG528950.056.4356771
CGCGTGT232900.053.313531
GTTTTTT7200900.050.146651
CGCCGTA612950.043.4472446-47
GAGCGGC54250.043.197159
GTCGCCG612050.042.56543444-45
CCGTATC634000.042.42841748-49
GCGTGTT295700.041.6665462
TGGTCGC615650.040.19326842-43
GATCTCG698350.039.92663634-35
TCGGTGG666950.039.7807838-39
AGAGCGT766600.038.7562269
TCTCGGT678650.038.25392536-37
AAGAGCG791100.038.1437238
GGTGGTC652800.038.0974240-41
CGTCGTG687550.037.71475212-13
AGCGTCG717900.037.6881810-11
ATCGGAA814700.037.2191163
TCGTGTA679950.037.11017614-15
GATCGGA816400.036.9372062
Produced by FastQC (version 0.11.5)