FastQCFastQC Report
Fri 27 May 2016
SRR1233088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38719552
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3032210.7831211476827005TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2904820.7502204570961979TruSeq Adapter, Index 3 (100% over 49bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1003980.2592953554834519No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT597690.15436387280513988No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT532000.137398283947087No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT528740.13655633205673454No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA505570.13057227521640746No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA477020.12319873948954782No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC469290.1212023320930986No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT464640.12000138844581673No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT446910.11542230653908392No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT421900.10896303758886466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG507600.060.427411
CGCGTGT223200.059.8835221
GTTTTTT6695650.049.5318261
GCGTGTT283900.046.8538132
TATGCCG765250.042.29679546-47
TGCCGTC767000.041.52348-49
CTCGTAT769000.041.360842-43
CCGTCTT776950.041.10994350-51
ATCTCGT758150.040.89263540-41
ATCGGAA878150.040.652882
GATCGGA881500.040.5005461
CGTCTGA809350.040.10934416-17
AGGCATC806850.038.69298636-37
CGGAAGA915650.038.6925664
CACACGT835950.038.5586912-13
TCGGAAG928250.038.2930033
ACTTAGG883700.037.1229932-33
CGTATGC878500.036.99173444-45
CACGTCT885650.036.543814-15
GCATCTC862200.036.39555738-39
Produced by FastQC (version 0.11.5)