FastQCFastQC Report
Fri 27 May 2016
SRR1233087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35628685
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6207911.7423909975908456Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1008010.2829209104966967No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT895330.25129470818246585No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT877300.2462341790049226No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT798480.22411155505739264No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT759470.21316251217242513No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT701370.1968554270246011No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT679580.19073956841236211No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG672140.18865136336073027No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT625080.17544290506371482No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT621390.1744072227195587No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC564470.15843133138368706No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT547550.15368234892755656No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC483210.13562386599449291No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT475120.13335322367356528No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA462840.12990656264748476No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA449670.12621010289882997No Hit
GTTTAATACGACTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAA446410.12529510982513106No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA442380.12416399875549715No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT438950.12320129131905934No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT437560.12281115623548834No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG404000.11339177968538551Illumina Single End PCR Primer 1 (100% over 50bp)
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT398210.111766684625043No Hit
GTCGTATTAAACCCTATAGTGAGTCGTATTAAACCCTATAGTGAGTCGTA391180.10979355538942848No Hit
CTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAATACGACTCACT390800.10968689975507095No Hit
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA389430.10930237812593982No Hit
ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG367730.10321178005868026No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA367120.10304056969826421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG991050.071.838411
AGAGCGT993700.070.792179
AAGAGCG1029800.068.9722068
GATCGGA1045750.068.1571962
ATCGGAA1051650.068.064633
TCGGAAG1049100.067.950084
CGGAAGA1052800.067.687555
GAAGAGC1189200.060.0265737
GGAAGAG1275000.055.976446
GTTTTTT5189200.046.4186971
CGCCGTA870350.043.94651846-47
CCGTATC881500.043.47781848-49
GTCGCCG870550.043.27892744-45
TGGTCGC858300.042.72683342-43
TCGGTGG892350.042.7187238-39
GGTGGTC907350.040.6083740-41
CGTATCA873600.040.567550-51
TCGCCGT852250.040.4027446-47
GGTCGCC864100.040.32301344-45
GTGGTCG855300.040.14985742-43
Produced by FastQC (version 0.11.5)