##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35628685 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15786072373987 34.0 31.0 34.0 30.0 34.0 2 32.378111120295344 34.0 33.0 34.0 31.0 34.0 3 32.403767694485495 34.0 31.0 34.0 31.0 34.0 4 35.655508560026846 37.0 37.0 37.0 35.0 37.0 5 35.62745661255811 37.0 37.0 37.0 35.0 37.0 6 35.65472088009984 37.0 37.0 37.0 35.0 37.0 7 35.67866223521862 37.0 37.0 37.0 35.0 37.0 8 35.65907686461063 37.0 37.0 37.0 35.0 37.0 9 37.40832868235243 39.0 39.0 39.0 35.0 39.0 10-11 37.34545779615498 39.0 39.0 39.0 35.0 39.0 12-13 37.29772300044192 39.0 38.0 39.0 35.0 39.0 14-15 38.69656526756461 41.0 39.0 41.0 35.5 41.0 16-17 38.515894383977404 41.0 39.0 41.0 35.0 41.0 18-19 38.06956188531797 40.5 38.0 41.0 34.0 41.0 20-21 37.75850879705496 40.0 38.0 41.0 33.0 41.0 22-23 37.42566761866176 40.0 38.0 41.0 32.5 41.0 24-25 36.97286043534865 40.0 38.0 41.0 31.5 41.0 26-27 36.61592069704509 40.0 38.0 41.0 29.5 41.0 28-29 36.374660740355694 40.5 38.0 41.0 27.0 41.0 30-31 36.067749006172974 40.0 37.5 41.0 24.5 41.0 32-33 35.90135963479988 40.0 37.5 41.0 24.0 41.0 34-35 35.75042574543517 40.0 37.0 41.0 23.0 41.0 36-37 35.604862023394915 40.0 37.0 41.0 21.5 41.0 38-39 35.469246058337546 40.0 37.0 41.0 19.0 41.0 40-41 35.32647376685387 40.0 37.0 41.0 17.5 41.0 42-43 35.10129705881651 40.0 36.0 41.0 15.5 41.0 44-45 34.80427373336961 40.0 35.5 41.0 9.5 41.0 46-47 34.64058912081656 40.0 35.0 41.0 9.0 41.0 48-49 34.589079950607214 40.0 35.0 41.0 8.5 41.0 50-51 34.0836893503086 39.0 34.5 40.5 7.5 41.0 52-53 34.06067696576508 39.5 35.0 40.5 3.5 41.0 54-55 34.39995249614181 40.0 35.0 41.0 2.0 41.0 56-57 34.096770369156204 40.0 35.0 41.0 2.0 41.0 58-59 33.92496642803404 40.0 35.0 41.0 2.0 41.0 60-61 33.89148726651011 39.5 35.0 41.0 2.0 41.0 62-63 33.68439159345903 39.0 35.0 41.0 2.0 41.0 64-65 33.3115506788982 39.0 34.5 41.0 2.0 41.0 66-67 32.615201304791356 38.5 34.0 41.0 2.0 41.0 68-69 32.136913613286595 37.5 33.5 40.5 2.0 41.0 70-71 31.769851469398887 37.0 33.0 40.0 2.0 41.0 72-73 31.385596493387283 37.0 33.0 39.0 2.0 41.0 74-75 30.972992505897984 36.0 32.0 39.0 2.0 41.0 76-77 30.564019567379486 36.0 32.0 39.0 2.0 40.5 78-79 30.049475485833955 35.0 31.0 37.5 2.0 39.5 80-81 29.64603355414324 35.0 31.0 37.0 2.0 39.0 82-83 29.31081374459933 35.0 30.5 37.0 2.0 39.0 84-85 28.947904616743504 35.0 30.0 36.0 2.0 38.0 86-87 28.594988041236995 35.0 30.0 36.0 2.0 37.0 88-89 28.256550094397255 35.0 29.0 35.5 2.0 37.0 90-91 27.93199280299006 35.0 29.0 35.0 2.0 36.5 92-93 27.635518852295558 35.0 28.5 35.0 2.0 36.0 94-95 27.321778841963997 35.0 27.5 35.0 2.0 36.0 96-97 27.01732319056962 34.0 26.5 35.0 2.0 36.0 98-99 26.70432606479863 34.0 25.5 35.0 2.0 35.5 100-101 25.181380297925674 32.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3330193886929891 1101 2 0.3006126938125391 1101 3 0.3688556912358365 1101 4 0.36171566348390627 1101 5 0.3884739146954246 1101 6 0.40814841976767013 1101 7 0.3529272042005047 1101 8 0.3337614693348172 1101 9 0.3680846900011048 1101 10-11 0.40297674109608295 1101 12-13 0.41937361385789274 1101 14-15 0.5127380754894872 1101 16-17 0.5161858650768636 1101 18-19 0.5884097200207989 1101 20-21 0.6153596739363252 1101 22-23 0.6986598084830717 1101 24-25 0.69601853012162 1101 26-27 0.6856565980133311 1101 28-29 0.6642380365681291 1101 30-31 0.6126819637742926 1101 32-33 0.6499201029919348 1101 34-35 0.6682810430243933 1101 36-37 0.6559223715264366 1101 38-39 0.6851626016334862 1101 40-41 0.6311202820935122 1101 42-43 0.631254790581302 1101 44-45 0.6654909059517138 1101 46-47 0.624263640319974 1101 48-49 0.7183745119111578 1101 50-51 0.7146640992897701 1101 52-53 0.7686877359110369 1101 54-55 0.8166660818994558 1101 56-57 0.7980694854438113 1101 58-59 0.7079293256242423 1101 60-61 0.7221207870992927 1101 62-63 0.7494475228247452 1101 64-65 0.644941151386142 1101 66-67 0.49484246667639553 1101 68-69 0.5628553237038005 1101 70-71 0.46847375919045575 1101 72-73 0.4507354836885398 1101 74-75 0.4703641657632964 1101 76-77 0.5391048059291954 1101 78-79 0.6420390755809251 1101 80-81 0.5871467572230458 1101 82-83 0.547095997521204 1101 84-85 0.5085457500016801 1101 86-87 0.6017261264098295 1101 88-89 0.6746273726648049 1101 90-91 0.6388141938630589 1101 92-93 0.7141435638942362 1101 94-95 0.78069562263574 1101 96-97 0.7654983495056022 1101 98-99 0.7907622921154669 1101 100-101 0.9782744442823947 1102 1 0.358740918805843 1102 2 0.32569266110751727 1102 3 0.34389956434356606 1102 4 0.3402196406039124 1102 5 0.3719993372019843 1102 6 0.3460209830831644 1102 7 0.31223830435379085 1102 8 0.26950144289718736 1102 9 0.34246481444498045 1102 10-11 0.3496113543543231 1102 12-13 0.40655465537845004 1102 14-15 0.4521017080688594 1102 16-17 0.4890017679183529 1102 18-19 0.5137458712845486 1102 20-21 0.4801746593790739 1102 22-23 0.4112844516482568 1102 24-25 0.4773730202850359 1102 26-27 0.550681027398987 1102 28-29 0.5470236738935412 1102 30-31 0.5221957756429774 1102 32-33 0.45050452554549025 1102 34-35 0.5224120884635397 1102 36-37 0.5871029543250401 1102 38-39 0.6200077075085773 1102 40-41 0.586321573269494 1102 42-43 0.5592520685079947 1102 44-45 0.6306433109650484 1102 46-47 0.5345593231276169 1102 48-49 0.5828792200296746 1102 50-51 0.6389010973723757 1102 52-53 0.7248173296888751 1102 54-55 0.7710330616669836 1102 56-57 0.6223214008881612 1102 58-59 0.6083507060079469 1102 60-61 0.5831114588869966 1102 62-63 0.6596020123726092 1102 64-65 0.6001881998112353 1102 66-67 0.530912143770415 1102 68-69 0.6151919403698898 1102 70-71 0.5882502305746122 1102 72-73 0.5557681218936459 1102 74-75 0.5530139671284893 1102 76-77 0.520616195531673 1102 78-79 0.6277978854777402 1102 80-81 0.6251304813530716 1102 82-83 0.6238938930560955 1102 84-85 0.6421298026462736 1102 86-87 0.6780939320221044 1102 88-89 0.7018104671051013 1102 90-91 0.7010126824453877 1102 92-93 0.7244847417016125 1102 94-95 0.8071741560876937 1102 96-97 0.806230278756388 1102 98-99 0.8208092607723501 1102 100-101 1.0020591300997062 1103 1 0.3118099753568444 1103 2 0.27665897530340544 1103 3 0.30171053413780413 1103 4 0.30999668831156413 1103 5 0.31894214719427083 1103 6 0.3094304588537682 1103 7 0.3119910396262071 1103 8 0.26439356606655906 1103 9 0.3372280952150959 1103 10-11 0.27893959511223443 1103 12-13 0.31024399787485635 1103 14-15 0.3263989295154701 1103 16-17 0.39024137270867953 1103 18-19 0.40131746008143665 1103 20-21 0.3882300746472396 1103 22-23 0.3799920708244713 1103 24-25 0.4029336548190372 1103 26-27 0.36385568007962377 1103 28-29 0.3370327729948457 1103 30-31 0.3584101509619302 1103 32-33 0.392339565825111 1103 34-35 0.394433619144003 1103 36-37 0.3870350620475449 1103 38-39 0.3791180584754983 1103 40-41 0.37949176680400143 1103 42-43 0.32306843984333966 1103 44-45 0.34315884098071336 1103 46-47 0.3557470597282162 1103 48-49 0.3842195901573433 1103 50-51 0.3797062774330371 1103 52-53 0.4628453542097972 1103 54-55 0.49403224433412873 1103 56-57 0.47061076877988484 1103 58-59 0.3984797764696921 1103 60-61 0.33581741820578515 1103 62-63 0.4088012896347877 1103 64-65 0.3989803346055609 1103 66-67 0.3742918600433356 1103 68-69 0.3979655301729039 1103 70-71 0.42398061882131444 1103 72-73 0.3941509142754356 1103 74-75 0.4172408540080106 1103 76-77 0.3799642898825759 1103 78-79 0.5016003685511663 1103 80-81 0.4599463556762622 1103 82-83 0.43170963605721724 1103 84-85 0.4604377153165977 1103 86-87 0.4862782474268599 1103 88-89 0.48055701401317563 1103 90-91 0.49565234224724364 1103 92-93 0.5198899849907441 1103 94-95 0.5732511867278838 1103 96-97 0.5432064496890661 1103 98-99 0.5437515166988653 1103 100-101 0.6720656761468895 1104 1 0.05000718884517141 1104 2 0.1454219424896408 1104 3 0.19419840830519774 1104 4 0.24294219674353457 1104 5 0.17743628228141972 1104 6 0.16853366558139982 1104 7 0.1853150271921109 1104 8 0.1675563553321453 1104 9 0.1766575254758962 1104 10-11 0.2126812535126561 1104 12-13 0.22924800498771702 1104 14-15 0.2462790478147312 1104 16-17 0.2431391244320693 1104 18-19 0.21448169832199682 1104 20-21 0.22405973703475013 1104 22-23 0.22916927372398277 1104 24-25 0.21803313689472503 1104 26-27 0.2789199387829058 1104 28-29 0.2571085987206132 1104 30-31 0.24539397840585764 1104 32-33 0.2703456619594746 1104 34-35 0.2776728505939303 1104 36-37 0.29605735610991246 1104 38-39 0.2818316820183213 1104 40-41 0.24497666763246428 1104 42-43 0.33072855868042694 1104 44-45 0.285856370527938 1104 46-47 0.28357491476226926 1104 48-49 0.2592698500313091 1104 50-51 0.2843324719536682 1104 52-53 0.4058227777525829 1104 54-55 0.45893228257041585 1104 56-57 0.46491130204628206 1104 58-59 0.37328046312394036 1104 60-61 0.30923479627202966 1104 62-63 0.3530133052646889 1104 64-65 0.3090610774639089 1104 66-67 0.31044877075205335 1104 68-69 0.31419178355111654 1104 70-71 0.30805247899397514 1104 72-73 0.28611262816769667 1104 74-75 0.2728580932290221 1104 76-77 0.3176875901070808 1104 78-79 0.4410773117121636 1104 80-81 0.40883123629968665 1104 82-83 0.3524925952658329 1104 84-85 0.32332338762347845 1104 86-87 0.334002415186319 1104 88-89 0.3842846788700882 1104 90-91 0.3726713250086675 1104 92-93 0.35631341327081145 1104 94-95 0.40023011620033344 1104 96-97 0.41623038446946836 1104 98-99 0.39125098762147914 1104 100-101 0.5289715615554975 1105 1 0.1693060432806206 1105 2 0.21976491127743714 1105 3 0.26992251069591333 1105 4 0.28407499023325755 1105 5 0.2671388758909856 1105 6 0.2689776376530446 1105 7 0.2547554882791516 1105 8 0.2059181785017259 1105 9 0.2790818404971276 1105 10-11 0.2745048349520829 1105 12-13 0.29334885661263144 1105 14-15 0.3711813965426316 1105 16-17 0.3755062949895276 1105 18-19 0.39381362387045016 1105 20-21 0.22738438295392172 1105 22-23 0.28870565855172003 1105 24-25 0.3915891994595455 1105 26-27 0.359273394715224 1105 28-29 0.40476773470737015 1105 30-31 0.45493599400283813 1105 32-33 0.4303300768499909 1105 34-35 0.49996155586084967 1105 36-37 0.4871833149933451 1105 38-39 0.5123669869486918 1105 40-41 0.5441579452010288 1105 42-43 0.5089648525899833 1105 44-45 0.5233482955691855 1105 46-47 0.41126187922023405 1105 48-49 0.4753726923944228 1105 50-51 0.49797236831635416 1105 52-53 0.5733141256877659 1105 54-55 0.6357703107395878 1105 56-57 0.5779876129734305 1105 58-59 0.5062191893981307 1105 60-61 0.467340066260185 1105 62-63 0.5155448474721922 1105 64-65 0.570716268139833 1105 66-67 0.5718295558264828 1105 68-69 0.5514510658217446 1105 70-71 0.525739389138618 1105 72-73 0.40042454473995903 1105 74-75 0.42998188115211633 1105 76-77 0.45820884736275147 1105 78-79 0.5614336875804398 1105 80-81 0.5633933895514325 1105 82-83 0.5100785397636649 1105 84-85 0.4818072373128608 1105 86-87 0.49389804258690617 1105 88-89 0.5037641124682963 1105 90-91 0.3928872796694378 1105 92-93 0.41893007303242413 1105 94-95 0.47611555511473824 1105 96-97 0.4814347139521793 1105 98-99 0.504309696849802 1105 100-101 0.47156449685548907 1106 1 0.07862277539937423 1106 2 0.1158885824842173 1106 3 0.10768240838404353 1106 4 0.16480978975342708 1106 5 0.1279337577449553 1106 6 0.022323416807417118 1106 7 0.07932308000529531 1106 8 0.09137675655893673 1106 9 0.10074709723451036 1106 10-11 0.06513210786442869 1106 12-13 0.09118493689495466 1106 14-15 0.07022720080926348 1106 16-17 -0.020858009633165864 1106 18-19 -0.017441647822231232 1106 20-21 0.07251822708553846 1106 22-23 0.05693120000529461 1106 24-25 0.06113977792816172 1106 26-27 0.06621829126347478 1106 28-29 -0.01646762068248364 1106 30-31 -0.03469110869125558 1106 32-33 -0.04209732586253523 1106 34-35 -6.45796153314393E-4 1106 36-37 -0.018592106252171448 1106 38-39 -0.007526339799554194 1106 40-41 -0.012335674739595959 1106 42-43 -0.032517394291652124 1106 44-45 -0.014688402234661169 1106 46-47 -0.07040842215862853 1106 48-49 -0.02793242876418134 1106 50-51 0.0012027251363733171 1106 52-53 0.06355164614945608 1106 54-55 0.12895096303094533 1106 56-57 0.06439459990821916 1106 58-59 0.020803063819990086 1106 60-61 -0.025394035738720788 1106 62-63 0.007567197064183517 1106 64-65 0.026682054620373208 1106 66-67 0.004051649823914261 1106 68-69 0.11389408818695301 1106 70-71 0.07955420033963989 1106 72-73 0.026781081715032684 1106 74-75 0.07008949868219005 1106 76-77 0.052282143986651874 1106 78-79 0.17063495436551435 1106 80-81 0.15025538681390316 1106 82-83 0.14235203338224522 1106 84-85 0.1510920687900672 1106 86-87 0.1579355967928926 1106 88-89 0.15833150705402232 1106 90-91 0.0911930602932749 1106 92-93 0.15982476859690564 1106 94-95 0.19329997950387323 1106 96-97 0.09926879184454762 1106 98-99 0.11324966034475281 1106 100-101 0.1709815586110075 1107 1 -0.05184589986269117 1107 2 -0.025219243823613624 1107 3 -0.012303153471471262 1107 4 0.04545301377208233 1107 5 -0.020772725904897982 1107 6 -0.028797889100026453 1107 7 -0.02531230805739426 1107 8 -0.04430116256288841 1107 9 -0.09646552435751232 1107 10-11 -0.03588782718614425 1107 12-13 -0.026469578869686927 1107 14-15 -0.11741002377829801 1107 16-17 -0.1238766847720072 1107 18-19 -0.13770238000511625 1107 20-21 -0.19954520217491734 1107 22-23 -0.10030435439415442 1107 24-25 -0.10282452406966769 1107 26-27 -0.14581262211497403 1107 28-29 -0.1344980344704041 1107 30-31 -0.15639825978026778 1107 32-33 -0.15252289286353715 1107 34-35 -0.14635336198024618 1107 36-37 -0.15206075584003287 1107 38-39 -0.16680374910104945 1107 40-41 -0.1765824385965189 1107 42-43 -0.18368215499310736 1107 44-45 -0.12977520434430545 1107 46-47 -0.19679332208968958 1107 48-49 -0.19709265461344216 1107 50-51 -0.256975039415515 1107 52-53 -0.14784940823280834 1107 54-55 -0.0782088363140403 1107 56-57 -0.14278288790639948 1107 58-59 -0.29841882974569245 1107 60-61 -0.3392319123295877 1107 62-63 -0.3526412804392507 1107 64-65 -0.32575432765568024 1107 66-67 -0.3103193851237833 1107 68-69 -0.2677399531883289 1107 70-71 -0.24916743702765487 1107 72-73 -0.30941601668743957 1107 74-75 -0.3416328484318605 1107 76-77 -0.2946226703611252 1107 78-79 -0.16449948970830164 1107 80-81 -0.22716022425959537 1107 82-83 -0.23451813683565703 1107 84-85 -0.25195782470281003 1107 86-87 -0.22479558886272955 1107 88-89 -0.20905371657527638 1107 90-91 -0.21068020524062092 1107 92-93 -0.19736271373486503 1107 94-95 -0.14735903298863562 1107 96-97 -0.1723949811359411 1107 98-99 -0.2572667267768729 1107 100-101 -0.2620655211654359 1108 1 0.004048968949895482 1108 2 0.07204235538954151 1108 3 0.09288090180582742 1108 4 0.09704338270643831 1108 5 0.038725391236219764 1108 6 0.008694428644218988 1108 7 0.06259525607703154 1108 8 0.06976836040406198 1108 9 0.031323856290271124 1108 10-11 2.178547327105207E-4 1108 12-13 0.011920592224477389 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-0.9224154235904471 2316 86-87 -0.9261466029727785 2316 88-89 -1.026794653543007 2316 90-91 -1.0054419439858968 2316 92-93 -0.9886833800391663 2316 94-95 -0.9664547682071323 2316 96-97 -0.9837743151513187 2316 98-99 -0.9675663070155451 2316 100-101 -1.1167686144842612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 256589.0 3 57028.0 4 53393.0 5 58087.0 6 77820.0 7 130859.0 8 259001.0 9 482520.0 10 610463.0 11 468400.0 12 322446.0 13 261967.0 14 232395.0 15 208614.0 16 194222.0 17 189893.0 18 193597.0 19 199459.0 20 215023.0 21 233070.0 22 263636.0 23 304351.0 24 396689.0 25 512804.0 26 280841.0 27 298189.0 28 326542.0 29 364466.0 30 414889.0 31 484967.0 32 588890.0 33 743635.0 34 999626.0 35 1500975.0 36 2525216.0 37 5514059.0 38 1.0767108E7 39 4613703.0 40 23253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.247024664372216 20.21622360427481 8.607607769822499 44.92914396153048 2 13.384256635734785 15.674661798631927 60.338038441218366 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36.07461383665377 16.850731326089782 22-23 20.45843587226904 28.24623431403398 34.37706328131842 16.91826653237856 24-25 18.409521818002872 29.487068709136455 34.58009832770202 17.52331114515865 26-27 17.475295174271686 30.757674210312803 34.01277632821617 17.75425428719935 28-29 18.438121534046907 30.742762571944187 33.11640572954973 17.702710164459173 30-31 18.81671720356637 29.109310492590172 33.92174893907395 18.15222336476951 32-33 19.35535687634762 29.688263372059197 33.18734599191095 17.76903375968223 34-35 19.790723098942948 30.764104743881397 32.113490480370245 17.33168167680541 36-37 18.90788777470998 30.06421428920914 33.67404509912083 17.35385283696005 38-39 18.853867874914897 29.31456191884537 33.426336261377074 18.40523394486266 40-41 19.865502858393754 28.45289887449344 33.27043002421333 18.411168242899475 42-43 20.193000100887254 28.696524306135746 33.586372272391316 17.524103320585688 44-45 20.88747573772039 28.952706362680498 32.73526122482906 17.424556674770052 46-47 18.015265101022628 28.686688244758727 34.28152465636 19.01652199785864 48-49 19.18523366990252 29.443817218704343 31.94535814002546 19.42559097136768 50-51 18.715041914493103 29.45233910457207 32.83312220279445 18.999496778140387 52-53 17.913267708947807 30.615626475428126 33.19253270215705 18.278573113467008 54-55 18.275573084752324 30.155481977979047 32.177011650222774 19.391933287045855 56-57 18.363475118161773 30.841491823063294 32.87928541169125 17.915747647083684 58-59 18.52444123738288 30.35083933374291 32.90531936948653 18.219400059387674 60-61 18.47178578857832 32.839095475234714 30.500377734033083 18.18874100215388 62-63 18.578271434271475 32.23748826403771 31.35811602432873 17.826124277362094 64-65 19.224309645287597 31.144109849666297 31.55984552329224 18.071734981753867 66-67 18.661857066154834 33.144124002523995 29.967323467825025 18.226695463496142 68-69 18.763510861467445 31.032593277179576 32.368166715870636 17.835729145482336 70-71 19.336538214256052 31.313145806389237 31.03289251698998 18.317423462364737 72-73 18.327742863848467 32.796509089723116 30.743429467478173 18.132318578950244 74-75 18.391751921671244 30.539149842981818 32.37928532954138 18.689812905805564 76-77 19.00583469914001 31.393801409959178 31.088409440344435 18.511954450556377 78-79 18.779321884558005 31.394944072862618 31.128353070358177 18.697380972221197 80-81 18.86522068186086 30.628453390525202 31.65813805695722 18.84818787065672 82-83 19.32884550354568 30.872563549674027 31.0661992110587 18.7323917357216 84-85 18.81204711279936 31.40600525611675 31.16814936674064 18.61379826434326 86-87 19.744652171701798 30.574116637937404 30.75859765979641 18.922633530564386 88-89 19.261181464173184 31.151406499481176 30.83754236692243 18.7498696694232 90-91 19.154898673344565 31.009693187227633 31.487069274935937 18.348338864491865 92-93 19.256859734984545 30.95004869202799 30.883852056439164 18.9092395165483 94-95 19.359933901047963 31.20152830480204 30.73999283350454 18.698544960645457 96-97 18.94314866714547 31.01143294984343 30.610507128928997 19.434911254082103 98-99 19.10194417794748 31.523873080578646 30.6764914698336 18.697691271640277 100-101 19.129648465737834 31.685260712371523 30.276990227269746 18.9081005946209 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28223.0 1 38543.5 2 44548.0 3 45516.5 4 56078.5 5 66420.5 6 75716.0 7 84243.5 8 92975.5 9 101184.0 10 108397.0 11 114926.0 12 123544.0 13 132122.5 14 140896.5 15 150814.0 16 159532.5 17 169962.0 18 188129.0 19 208131.5 20 233433.0 21 269099.5 22 308661.5 23 357847.5 24 418370.0 25 481948.0 26 547998.0 27 630973.5 28 734765.5 29 829959.5 30 1005795.5 31 1157114.0 32 1349142.5 33 1471980.5 34 1350125.5 35 1360946.0 36 1518506.0 37 1650480.0 38 1822871.0 39 1678517.5 40 1486546.0 41 1334897.5 42 1098906.0 43 1038265.5 44 939494.5 45 869703.5 46 833373.5 47 789764.5 48 737886.0 49 689948.0 50 615226.5 51 535725.0 52 473228.5 53 429522.5 54 397098.5 55 355951.5 56 321901.5 57 276129.5 58 234459.5 59 198881.0 60 153924.5 61 117926.0 62 91735.0 63 66158.5 64 43229.5 65 28989.5 66 20365.5 67 14939.5 68 10613.5 69 7114.0 70 8164.5 71 12799.5 72 13273.0 73 21187.0 74 22373.5 75 10985.0 76 7247.5 77 10166.5 78 9483.0 79 3481.0 80 1581.5 81 655.5 82 318.5 83 180.5 84 126.0 85 103.5 86 89.5 87 64.5 88 46.0 89 34.5 90 26.0 91 21.0 92 15.5 93 10.5 94 7.0 95 2.5 96 1.5 97 4.0 98 3.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44553145871086736 2 0.34229161137998776 3 0.318992968727305 4 0.3901070162988053 5 0.35638138202406294 6 0.2834878693951236 7 0.2589346196751297 8 0.26338889577316704 9 0.2552578070170145 10-11 0.25797471896591184 12-13 0.23400105841683466 14-15 0.22337198243493972 16-17 0.20841773980712452 18-19 0.2428492659776806 20-21 0.259964688564846 22-23 0.21824689853133789 24-25 0.24567564028815544 26-27 0.24445611731109357 28-29 0.2190313787893098 30-31 0.23122099510548877 32-33 0.16765564039200437 34-35 0.22264223335775654 36-37 0.2144213293305661 38-39 0.20386943834721938 40-41 0.22884790724103346 42-43 0.2081019829948818 44-45 0.4882526537255023 46-47 0.13081594226674378 48-49 0.15664625287180822 50-51 0.15691289195770206 52-53 0.3722618446344568 54-55 0.2604586725555546 56-57 0.4171343960631721 58-59 0.3783145518842472 60-61 0.12755733196439892 62-63 0.14036162154174367 64-65 0.09124529855648617 66-67 0.12084223709070373 68-69 0.14201057378345566 70-71 0.13926980465318886 72-73 0.17249443811917278 74-75 0.17252110202776216 76-77 0.15545199043972574 78-79 0.21568997003397683 80-81 0.14940349328076521 82-83 0.1374692891416004 84-85 0.15567372189010065 86-87 0.13945645201331455 88-89 0.12936486429403724 90-91 0.19979828051470325 92-93 0.19500018033222388 94-95 0.216505324291368 96-97 0.1666831094102968 98-99 0.18363854854592584 100-101 0.17304596001789008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.5628685E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.889332724038415 #Duplication Level Percentage of deduplicated Percentage of total 1 75.66521158865041 15.805957805100856 2 9.181705788124694 3.8359941436473215 3 3.737857846087854 2.3424406866626026 4 2.180953621604414 1.8223466342956474 5 1.3607505634099861 1.4212585636746968 6 0.9852948188596351 1.2349290781458049 7 0.7598176997673143 1.111045931803707 8 0.5928413744769246 0.9907248579219787 9 0.4946599345591624 0.9299804362431673 >10 3.9887544134945765 17.00941063892901 >50 0.5581678013884577 8.165743672771258 >100 0.42702695517542477 17.557195702860177 >500 0.03971300102131552 5.6805804995849005 >1k 0.023898151630634495 9.518756971478785 >5k 0.0019161077757529922 2.7066458011695427 >10k+ 0.0014303339733427302 9.866988575710462 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 620791 1.7423909975908456 Illumina Single End PCR Primer 1 (100% over 50bp) CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 100801 0.2829209104966967 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89533 0.25129470818246585 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 87730 0.2462341790049226 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 79848 0.22411155505739264 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 75947 0.21316251217242513 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 70137 0.1968554270246011 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 67958 0.19073956841236211 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 67214 0.18865136336073027 No Hit CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT 62508 0.17544290506371482 No Hit ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT 62139 0.1744072227195587 No Hit CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC 56447 0.15843133138368706 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 54755 0.15368234892755656 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 48321 0.13562386599449291 No Hit CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT 47512 0.13335322367356528 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 46284 0.12990656264748476 No Hit ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA 44967 0.12621010289882997 No Hit GTTTAATACGACTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAA 44641 0.12529510982513106 No Hit ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA 44238 0.12416399875549715 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 43895 0.12320129131905934 No Hit CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT 43756 0.12281115623548834 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 40400 0.11339177968538551 Illumina Single End PCR Primer 1 (100% over 50bp) ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 39821 0.111766684625043 No Hit GTCGTATTAAACCCTATAGTGAGTCGTATTAAACCCTATAGTGAGTCGTA 39118 0.10979355538942848 No Hit CTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAATACGACTCACT 39080 0.10968689975507095 No Hit CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA 38943 0.10930237812593982 No Hit ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG 36773 0.10321178005868026 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 36712 0.10304056969826421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.040684353071128 0.0 0.0 2.8067272199352852E-6 0.0 2 2.0461181769689225 0.0 0.0 5.6134544398705704E-6 0.0 3 2.0462809671476787 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 4 2.0682464143708925 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 5 2.0689368692669965 2.8067272199352852E-6 0.0 1.1226908879741141E-5 0.0 6 2.0695066348926434 2.8067272199352852E-6 0.0 1.1226908879741141E-5 0.0 7 2.0712411923145635 2.8067272199352852E-6 0.0 1.1226908879741141E-5 0.0 8 2.0817271252082414 2.8067272199352852E-6 0.0 1.1226908879741141E-5 0.0 9 2.0822211091989504 2.8067272199352852E-6 0.0 1.1226908879741141E-5 0.0 10-11 2.0881068161791547 2.8067272199352852E-6 0.0 1.1226908879741141E-5 0.0 12-13 2.0928782524530445 2.8067272199352852E-6 0.0 1.2630272489708783E-5 0.0 14-15 2.095596567765552 5.6134544398705704E-6 0.0 1.4033636099676426E-5 0.0 16-17 2.099318288059186 5.6134544398705704E-6 0.0 1.4033636099676426E-5 0.0 18-19 2.103141050532738 5.6134544398705704E-6 0.0 1.543699970964407E-5 0.0 20-21 2.1080584366220645 5.6134544398705704E-6 0.0 1.6840363319611712E-5 0.0 22-23 2.112292384633337 5.6134544398705704E-6 0.0 1.6840363319611712E-5 0.0 24-25 2.1157839532949363 5.6134544398705704E-6 0.0 1.8243726929579355E-5 0.0 26-27 2.1191197485958293 5.6134544398705704E-6 0.0 2.3857181369449924E-5 0.0 28-29 2.122497644805022 5.6134544398705704E-6 0.0 2.806727219935285E-5 0.0 30-31 2.1259176419225128 8.420181659805856E-6 0.0 3.087399941928814E-5 0.0 32-33 2.12951025276403 1.1226908879741141E-5 0.0 3.3680726639223424E-5 0.0 34-35 2.133535099597417 1.1226908879741141E-5 0.0 3.648745385915871E-5 0.0 36-37 2.1371684079836233 1.1226908879741141E-5 0.0 3.9294181079093994E-5 0.0 38-39 2.1411820279081306 1.4033636099676426E-5 0.0 4.0697544689061636E-5 0.0 40-41 2.1454257994646726 1.4033636099676426E-5 2.8067272199352852E-6 4.771436273889985E-5 0.0 42-43 2.149837974654411 1.4033636099676426E-5 2.8067272199352852E-6 5.052108995883513E-5 0.0 44-45 2.1556422865452376 1.4033636099676426E-5 2.8067272199352852E-6 6.0344635228608637E-5 0.0 46-47 2.165273571000445 1.4033636099676426E-5 2.8067272199352852E-6 6.455472605851156E-5 0.0 48-49 2.170680730989651 1.4033636099676426E-5 2.8067272199352852E-6 6.736145327844685E-5 0.0 50-51 2.1760556136158264 1.4033636099676426E-5 2.8067272199352852E-6 6.736145327844685E-5 0.0 52-53 2.182209363045535 1.4033636099676426E-5 2.8067272199352852E-6 6.736145327844685E-5 0.0 54-55 2.188737810559104 1.4033636099676426E-5 2.8067272199352852E-6 7.016818049838213E-5 0.0 56-57 2.1955371072493977 1.4033636099676426E-5 2.8067272199352852E-6 7.016818049838213E-5 0.0 58-59 2.202935640201147 1.4033636099676426E-5 2.8067272199352852E-6 7.297490771831742E-5 0.0 60-61 2.2109614766865517 1.4033636099676426E-5 2.8067272199352852E-6 7.297490771831742E-5 0.0 62-63 2.219551465343164 1.4033636099676426E-5 2.8067272199352852E-6 7.297490771831742E-5 0.0 64-65 2.22840809308567 1.6840363319611712E-5 2.8067272199352852E-6 7.437827132828506E-5 0.0 66-67 2.238291982990672 1.6840363319611712E-5 2.8067272199352852E-6 7.57816349382527E-5 0.0 68-69 2.249607303777841 1.6840363319611712E-5 2.8067272199352852E-6 8.139508937812327E-5 0.0 70-71 2.260602657661937 1.6840363319611712E-5 2.8067272199352852E-6 8.420181659805856E-5 0.0 72-73 2.2729592742477025 1.6840363319611712E-5 2.8067272199352852E-6 8.56051802080262E-5 0.0 74-75 2.286775388987834 1.6840363319611712E-5 2.8067272199352852E-6 9.121863464789677E-5 0.0 76-77 2.301735245070089 1.6840363319611712E-5 2.8067272199352852E-6 1.0805899796750848E-4 0.0 78-79 2.3179777754918542 1.6840363319611712E-5 2.8067272199352852E-6 1.1367245240737905E-4 0.0 80-81 2.3361822082403547 1.6840363319611712E-5 2.8067272199352852E-6 1.1928590684724963E-4 0.0 82-83 2.3565730814931847 1.6840363319611712E-5 2.8067272199352852E-6 1.2349599767715256E-4 0.0 84-85 2.380374128318236 1.6840363319611712E-5 2.8067272199352852E-6 1.2770608850705547E-4 0.0 86-87 2.40750535698974 1.6840363319611712E-5 2.8067272199352852E-6 1.319161793369584E-4 0.0 88-89 2.4386417854040925 1.9647090539546997E-5 2.8067272199352852E-6 1.319161793369584E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 99105 0.0 71.83841 1 AGAGCGT 99370 0.0 70.79217 9 AAGAGCG 102980 0.0 68.972206 8 GATCGGA 104575 0.0 68.157196 2 ATCGGAA 105165 0.0 68.06463 3 TCGGAAG 104910 0.0 67.95008 4 CGGAAGA 105280 0.0 67.68755 5 GAAGAGC 118920 0.0 60.026573 7 GGAAGAG 127500 0.0 55.97644 6 GTTTTTT 518920 0.0 46.418697 1 CGCCGTA 87035 0.0 43.946518 46-47 CCGTATC 88150 0.0 43.477818 48-49 GTCGCCG 87055 0.0 43.278927 44-45 TGGTCGC 85830 0.0 42.726833 42-43 TCGGTGG 89235 0.0 42.71872 38-39 GGTGGTC 90735 0.0 40.60837 40-41 CGTATCA 87360 0.0 40.5675 50-51 TCGCCGT 85225 0.0 40.40274 46-47 GGTCGCC 86410 0.0 40.323013 44-45 GTGGTCG 85530 0.0 40.149857 42-43 >>END_MODULE