FastQCFastQC Report
Fri 27 May 2016
SRR1233087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35628685
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG6569191.8437924385926678TruSeq Adapter, Index 2 (100% over 49bp)
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1029360.28891327311125853No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT847010.2377326022557386No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT835510.23450486595281303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC829800.23290222471022995TruSeq Adapter, Index 2 (100% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT747820.20989267496120048No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT728340.20442517033676658No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT696390.19545767686907334No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG679430.19069746750406308No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT671280.18840998481981583No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT667740.18741640338395874No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT628410.17637754522795326No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT571540.1604156875281813No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC566830.15909371900759178No Hit
ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA477670.1340689391146488No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA469310.13172251515878286No Hit
CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT460650.12929188938631891No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC459730.12903367048208486No Hit
ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA436780.12259223151233338No Hit
CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT434800.1220364995227862No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT423780.11894348612641753No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT410620.1152498331049827No Hit
CTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAATACGACTCACT398250.11177791153392273No Hit
GTCGTATTAAACCCTATAGTGAGTCGTATTAAACCCTATAGTGAGTCGTA391870.10998721956760403No Hit
GTTTAATACGACTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAA384630.10795514906037088No Hit
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA369470.10370015059494898No Hit
ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG365760.10265885479635299No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA364320.10225468607668231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG969900.074.682851
GATCGGA1048000.069.323262
ATCGGAA1051850.068.983023
TCGGAAG1055350.068.6795654
CGGAAGA1060500.068.223615
AGAGCAC1158100.062.4056789
GAAGAGC1196950.060.376757
GGAAGAG1269000.057.166966
AAGAGCA1348800.053.7892468
GTTTTTT4649250.048.0601041
ACCGATG937950.042.83612432-33
CGATGTA926350.042.47907334-35
TATGCCG944800.042.33021546-47
TCACCGA946600.042.1350330-31
TGCCGTC942750.042.0471948-49
CCGTCTT953750.041.6748350-51
CTCGTAT957800.041.2025542-43
ATCTCGT968050.040.4407640-41
CGTCTGA999100.040.42042516-17
GTATCTC963650.040.2737138-39
Produced by FastQC (version 0.11.5)