##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35628685 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43038411325032 34.0 31.0 34.0 28.0 34.0 2 31.71809537736237 34.0 31.0 34.0 29.0 34.0 3 31.830169286348905 34.0 31.0 34.0 29.0 34.0 4 35.03885868928365 37.0 37.0 37.0 32.0 37.0 5 34.9087395731838 37.0 35.0 37.0 32.0 37.0 6 34.88638368213702 37.0 36.0 37.0 32.0 37.0 7 34.89704660724919 37.0 35.0 37.0 32.0 37.0 8 34.84056610565335 37.0 35.0 37.0 32.0 37.0 9 36.56986588194316 39.0 38.0 39.0 33.0 39.0 10-11 36.55128728438897 39.0 38.0 39.0 32.5 39.0 12-13 36.503588274447964 39.0 38.0 39.0 32.5 39.0 14-15 37.932678514517164 41.0 39.0 41.0 33.0 41.0 16-17 37.79246148152816 41.0 39.0 41.0 33.0 41.0 18-19 37.38317106006018 41.0 38.0 41.0 32.0 41.0 20-21 37.06554982312707 41.0 38.0 41.0 31.5 41.0 22-23 36.82254648185865 41.0 38.0 41.0 30.5 41.0 24-25 36.48689839380825 41.0 38.0 41.0 28.5 41.0 26-27 35.99693391715131 41.0 38.0 41.0 24.5 41.0 28-29 35.707452534383464 40.0 38.0 41.0 19.5 41.0 30-31 35.36974234384458 40.0 37.5 41.0 15.5 41.0 32-33 35.29837385241695 40.0 38.0 41.0 9.5 41.0 34-35 35.21747636770765 40.5 38.0 41.0 8.5 41.0 36-37 35.11199854836068 40.0 37.5 41.0 7.0 41.0 38-39 35.00854054815663 40.0 37.0 41.0 3.5 41.0 40-41 34.840192670596736 40.0 37.0 41.0 2.0 41.0 42-43 34.74319158846306 40.0 37.0 41.0 2.0 41.0 44-45 34.673458155977414 40.0 37.0 41.0 2.0 41.0 46-47 34.53598693019403 40.0 36.0 41.0 2.0 41.0 48-49 34.45586153404202 40.0 36.0 41.0 2.0 41.0 50-51 34.308254570720194 40.0 36.0 41.0 2.0 41.0 52-53 34.14261424467392 40.0 35.0 41.0 2.0 41.0 54-55 34.03347502160128 40.0 35.0 41.0 2.0 41.0 56-57 34.0032360442155 40.0 35.0 41.0 2.0 41.0 58-59 33.83320276906094 40.0 35.0 41.0 2.0 41.0 60-61 33.632088526421896 40.0 35.0 41.0 2.0 41.0 62-63 33.37526853713518 39.0 35.0 41.0 2.0 41.0 64-65 32.98917056579551 39.0 34.5 41.0 2.0 41.0 66-67 32.76672972914942 39.0 34.0 41.0 2.0 41.0 68-69 32.33519638740526 38.0 34.0 40.5 2.0 41.0 70-71 31.9294109928559 37.0 33.5 40.0 2.0 41.0 72-73 31.395589101871145 37.0 33.0 39.5 2.0 41.0 74-75 30.88447909318012 36.0 33.0 39.0 2.0 41.0 76-77 29.768538187699043 35.0 30.5 38.0 2.0 39.0 78-79 30.101710952846 35.0 32.0 37.5 2.0 39.5 80-81 29.909999077990108 35.0 32.0 37.0 2.0 39.0 82-83 29.59319371455893 35.0 32.0 37.0 2.0 39.0 84-85 29.296067873400325 35.0 32.0 36.5 2.0 38.0 86-87 29.001340577122058 35.0 31.0 36.0 2.0 37.0 88-89 28.737809759187016 35.0 31.0 36.0 2.0 37.0 90-91 28.50389836167122 35.0 31.0 35.0 2.0 36.5 92-93 28.278202816073623 35.0 31.0 35.0 2.0 36.0 94-95 28.077244894107096 35.0 30.5 35.0 2.0 36.0 96-97 27.87772424101535 35.0 30.0 35.0 2.0 36.0 98-99 27.65208777702573 35.0 30.0 35.0 2.0 36.0 100-101 26.76041537878819 34.0 26.5 35.0 2.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34036644843248 1101 2 0.34317613482351916 1101 3 0.34574030700236946 1101 4 0.2937847516740817 1101 5 0.3754222679621364 1101 6 0.4090369772042024 1101 7 0.4139578403095072 1101 8 0.24467253320110416 1101 9 0.3347164334023134 1101 10-11 0.3885188554450991 1101 12-13 0.43214262820803384 1101 14-15 0.5951179783125866 1101 16-17 0.6106217118418371 1101 18-19 0.6550925575091142 1101 20-21 0.6406577098693305 1101 22-23 0.6607852536989114 1101 24-25 0.6601160084393172 1101 26-27 0.7409841534187507 1101 28-29 0.7037806374824669 1101 30-31 0.6683063080583835 1101 32-33 0.6683553437835457 1101 34-35 0.6553764477084698 1101 36-37 0.5805813360123722 1101 38-39 0.6786633551135424 1101 40-41 0.673464138645798 1101 42-43 0.6861081284700887 1101 44-45 0.6863744948215498 1101 46-47 0.6619718707303974 1101 48-49 0.5559302509981592 1101 50-51 0.6438954319986223 1101 52-53 0.6763189207790745 1101 54-55 0.6736980126120002 1101 56-57 0.652794494276506 1101 58-59 0.7061590444071655 1101 60-61 0.6394800152476492 1101 62-63 0.6659126670466193 1101 64-65 0.6777780792490162 1101 66-67 0.7079460053472033 1101 68-69 0.7235176126620004 1101 70-71 0.445490773425572 1101 72-73 0.3904991486267484 1101 74-75 0.36068631863594547 1101 76-77 0.343093830901978 1101 78-79 0.4266681429861521 1101 80-81 0.40345217356452423 1101 82-83 0.4322510572431497 1101 84-85 0.4408214511634405 1101 86-87 0.464507621247332 1101 88-89 0.47030437533029 1101 90-91 0.4923990634135187 1101 92-93 0.5265574306855783 1101 94-95 0.49725076639823484 1101 96-97 0.48196380738821887 1101 98-99 0.500519339552504 1101 100-101 0.6365887298589676 1102 1 0.32591520955728726 1102 2 0.306549065862864 1102 3 0.31122514320427896 1102 4 0.3033668360486601 1102 5 0.3444872427648704 1102 6 0.3562771259722908 1102 7 0.3727436308758527 1102 8 0.269827514353679 1102 9 0.3273053788207889 1102 10-11 0.32630273745866134 1102 12-13 0.41909120935790156 1102 14-15 0.48916163533547774 1102 16-17 0.49866929266102034 1102 18-19 0.49990341897690627 1102 20-21 0.48251382507682905 1102 22-23 0.4743303566292454 1102 24-25 0.4764727285408128 1102 26-27 0.6330284241842108 1102 28-29 0.5923779016544302 1102 30-31 0.6565183113370026 1102 32-33 0.5056552108929111 1102 34-35 0.48686954917928915 1102 36-37 0.540061407353754 1102 38-39 0.5804799802677039 1102 40-41 0.6002151826894533 1102 42-43 0.634209462623545 1102 44-45 0.6551280851712278 1102 46-47 0.6191557549853073 1102 48-49 0.5920803261515104 1102 50-51 0.5168443255638877 1102 52-53 0.47477257935441486 1102 54-55 0.5732102727883515 1102 56-57 0.6170007594032612 1102 58-59 0.6475877713873999 1102 60-61 0.6405864160308923 1102 62-63 0.6669251547092614 1102 64-65 0.6657874892383546 1102 66-67 0.6430941277755053 1102 68-69 0.7177729167859681 1102 70-71 0.53993423320156 1102 72-73 0.35367722216910735 1102 74-75 0.3439403356102453 1102 76-77 0.08078243361487836 1102 78-79 0.41634391074035193 1102 80-81 0.43938016262411494 1102 82-83 0.38033670910948203 1102 84-85 0.3995049052117885 1102 86-87 0.46188876301374293 1102 88-89 0.48471100735446626 1102 90-91 0.45843652339704377 1102 92-93 0.4559206826647184 1102 94-95 0.49394556635932574 1102 96-97 0.4958762075056562 1102 98-99 0.48546688550963424 1102 100-101 0.5463389649987427 1103 1 0.2625692798097461 1103 2 0.25128855656875615 1103 3 0.23053444626578212 1103 4 0.19278939896037173 1103 5 0.2519511936573764 1103 6 0.31498618493550623 1103 7 0.26911730033208414 1103 8 0.224842071685039 1103 9 0.2978551269028884 1103 10-11 0.31066960442275615 1103 12-13 0.30511196249869244 1103 14-15 0.3932295942540236 1103 16-17 0.39542716006906176 1103 18-19 0.39407247917295507 1103 20-21 0.3803036320078661 1103 22-23 0.38884692163235 1103 24-25 0.3841215863115721 1103 26-27 0.4587407278797073 1103 28-29 0.3965381932194987 1103 30-31 0.38805247867814785 1103 32-33 0.39699385588955494 1103 34-35 0.40580352456807844 1103 36-37 0.4158151748179151 1103 38-39 0.37095086567334334 1103 40-41 0.3709832159113944 1103 42-43 0.39537928560586266 1103 44-45 0.4076096080440834 1103 46-47 0.333985001125086 1103 48-49 0.28910655368881066 1103 50-51 0.3569912543376219 1103 52-53 0.3843761705624189 1103 54-55 0.40827128999178797 1103 56-57 0.3880382573609964 1103 58-59 0.46595143781866 1103 60-61 0.4896290754335624 1103 62-63 0.5041092051451983 1103 64-65 0.49193658428679754 1103 66-67 0.44936467134872515 1103 68-69 0.51011904194403 1103 70-71 0.36871940791118973 1103 72-73 0.31215701146306074 1103 74-75 0.3935490032981228 1103 76-77 0.3735436933045193 1103 78-79 0.457014508854094 1103 80-81 0.4207041288130604 1103 82-83 0.40914762479775035 1103 84-85 0.42983941744542875 1103 86-87 0.453013849735612 1103 88-89 0.4159635023103867 1103 90-91 0.4073427160324279 1103 92-93 0.41862476536793025 1103 94-95 0.44600400593988 1103 96-97 0.4269083068699224 1103 98-99 0.4914045512718346 1103 100-101 0.513285346045663 1104 1 0.15591881505746485 1104 2 0.17169052059387369 1104 3 0.17086452712493028 1104 4 0.13669620152455053 1104 5 0.1292442494068382 1104 6 0.16271225558958946 1104 7 0.15771485179563882 1104 8 0.1039320414019258 1104 9 0.13146148156663173 1104 10-11 0.15098639805587766 1104 12-13 0.19531727491797568 1104 14-15 0.20585044375508232 1104 16-17 0.09874929693779677 1104 18-19 0.18576315281549682 1104 20-21 0.1765231023596172 1104 22-23 0.20040710890096847 1104 24-25 0.23181002193859257 1104 26-27 0.31786290078451174 1104 28-29 0.24708229224085443 1104 30-31 0.34621655404942686 1104 32-33 0.32290090728363197 1104 34-35 0.31943038424287806 1104 36-37 0.2838623529936726 1104 38-39 0.19769330432663423 1104 40-41 0.19501299568371877 1104 42-43 0.2391159244609824 1104 44-45 0.16783260218666385 1104 46-47 0.27990180551485366 1104 48-49 0.23431980573771227 1104 50-51 0.27785883949893275 1104 52-53 0.2919810856335161 1104 54-55 0.32067539120543387 1104 56-57 0.20278781825815173 1104 58-59 0.3083165620681001 1104 60-61 0.34108437686825255 1104 62-63 0.277737464240424 1104 64-65 0.2601417060201854 1104 66-67 0.2879195398286427 1104 68-69 0.36361325532736544 1104 70-71 0.31763909895246556 1104 72-73 0.3058133653438446 1104 74-75 0.30221682707448494 1104 76-77 0.046666918880056585 1104 78-79 0.3054482151437625 1104 80-81 0.3110068919019575 1104 82-83 0.2916306274227587 1104 84-85 0.28938805017930136 1104 86-87 0.3175915257290818 1104 88-89 0.3251817746974659 1104 90-91 0.3550968147386122 1104 92-93 0.395431641451232 1104 94-95 0.3686331991052896 1104 96-97 0.3429444987364043 1104 98-99 0.31197674507874495 1104 100-101 0.348962588555775 1105 1 0.07356525348465937 1105 2 0.12795737706238697 1105 3 0.08844864155964416 1105 4 0.08465434639709457 1105 5 0.08912361815508518 1105 6 0.16240169015670602 1105 7 0.15058403668118814 1105 8 0.03516647920249483 1105 9 0.08770744363644667 1105 10-11 0.12832661957791203 1105 12-13 0.19301904469619302 1105 14-15 0.23092984813229123 1105 16-17 0.2443099516540599 1105 18-19 0.2500129917166376 1105 20-21 0.26247679162137416 1105 22-23 0.25472029364954807 1105 24-25 0.19814581398225783 1105 26-27 0.3045117211861381 1105 28-29 0.18316894634976677 1105 30-31 0.2615684293054841 1105 32-33 0.2628300058260038 1105 34-35 0.31233424742519134 1105 36-37 0.30962231696136655 1105 38-39 0.29658516691981873 1105 40-41 0.2970821030418733 1105 42-43 0.2703463439414904 1105 44-45 0.2779579060428148 1105 46-47 0.2641032107843415 1105 48-49 0.24674467348532403 1105 50-51 0.2595217614197409 1105 52-53 0.27676861889042215 1105 54-55 0.275336430040916 1105 56-57 0.29233575667567635 1105 58-59 0.32858393910777295 1105 60-61 0.32876090047424356 1105 62-63 0.330810842536863 1105 64-65 0.3294431531412343 1105 66-67 0.18887669702827026 1105 68-69 0.345007554247978 1105 70-71 0.31177366082757985 1105 72-73 0.27907824937333103 1105 74-75 0.20330504094218682 1105 76-77 0.12396556366465461 1105 78-79 0.10949886207561832 1105 80-81 0.08860882860671282 1105 82-83 0.17245579128398347 1105 84-85 0.22067135698361895 1105 86-87 0.2590898406421367 1105 88-89 0.23188219318483405 1105 90-91 0.2101590385796399 1105 92-93 0.2554108067755436 1105 94-95 0.2563086631327174 1105 96-97 0.22177140274444085 1105 98-99 0.24191300872358568 1105 100-101 0.24993577575805404 1106 1 0.0340266424176221 1106 2 0.08655944102607194 1106 3 0.03186508344213834 1106 4 -0.005265841668688154 1106 5 0.004201516750605094 1106 6 0.021013053134815607 1106 7 -0.020587158135029426 1106 8 0.01774473070431526 1106 9 0.0278496647916171 1106 10-11 0.00978398829714422 1106 12-13 -0.014944184854073228 1106 14-15 -0.030023136004814432 1106 16-17 0.004413544841156636 1106 18-19 -0.03592462048985823 1106 20-21 -0.051610263998902894 1106 22-23 -0.08093977038425493 1106 24-25 -0.06398159136471548 1106 26-27 -0.04999223068491432 1106 28-29 -0.0965591726573436 1106 30-31 -0.051510068930404884 1106 32-33 -0.059571956056622355 1106 34-35 -0.08858217914495725 1106 36-37 -0.12808216214575907 1106 38-39 -0.062404380049194685 1106 40-41 -0.1006064088073444 1106 42-43 -0.11588924899501052 1106 44-45 -0.10539087827204696 1106 46-47 -0.05107235405004218 1106 48-49 -0.06382123236528514 1106 50-51 -0.052770518111771025 1106 52-53 -0.0477410346911924 1106 54-55 -0.07917515404628261 1106 56-57 -0.02077832268142288 1106 58-59 -0.031163899324340605 1106 60-61 -0.011131218222722339 1106 62-63 0.05194899516150997 1106 64-65 0.0679485947977696 1106 66-67 0.10342026040311936 1106 68-69 0.14469617873573526 1106 70-71 0.0011058039045757084 1106 72-73 -0.02242157760197827 1106 74-75 0.0026146286737791513 1106 76-77 -0.06358837414759577 1106 78-79 0.0029354909925736195 1106 80-81 -0.0337094441155017 1106 82-83 -0.03672192040496114 1106 84-85 0.007288592238463565 1106 86-87 0.029082435764404835 1106 88-89 0.03374277071461407 1106 90-91 -0.010652027038769774 1106 92-93 0.05474916860536183 1106 94-95 0.0800168578673528 1106 96-97 0.04435376288104109 1106 98-99 0.07575369317673974 1106 100-101 0.05914618662868776 1107 1 -0.07857212107326106 1107 2 -0.07921853072194196 1107 3 -0.05726155378296838 1107 4 -0.030739896049830406 1107 5 -0.044483367232245996 1107 6 -0.026752264044986873 1107 7 -0.03060640362873812 1107 8 -0.04844917506519408 1107 9 -0.09963565908959993 1107 10-11 -0.09966179361134664 1107 12-13 -0.10613973342618976 1107 14-15 -0.16361985938153367 1107 16-17 -0.1809749164732395 1107 18-19 -0.13679995519768084 1107 20-21 -0.16346752391791597 1107 22-23 -0.20987101459720492 1107 24-25 -0.18814370232374955 1107 26-27 -0.11177618236263243 1107 28-29 -0.16358679309441726 1107 30-31 -0.12889301103582085 1107 32-33 -0.1241872309637273 1107 34-35 -0.23306466270485515 1107 36-37 -0.26954673884620206 1107 38-39 -0.269465446945496 1107 40-41 -0.24872435815531446 1107 42-43 -0.2572769526472811 1107 44-45 -0.18942566026486674 1107 46-47 -0.2141761066822312 1107 48-49 -0.2129919747153366 1107 50-51 -0.1977954268644666 1107 52-53 -0.21351767690750734 1107 54-55 -0.33046246623171527 1107 56-57 -0.2535391405122738 1107 58-59 -0.24723897511156423 1107 60-61 -0.21654725316992796 1107 62-63 -0.20123879997107963 1107 64-65 -0.13780092801074062 1107 66-67 -0.11903115113670992 1107 68-69 -0.1346900457080551 1107 70-71 -0.22473524253677724 1107 72-73 -0.21998886744500012 1107 74-75 -0.18312425636165486 1107 76-77 -0.2233190621167651 1107 78-79 -0.1160018087149588 1107 80-81 -0.12151648646354118 1107 82-83 -0.10465714850742103 1107 84-85 -0.14029410663957265 1107 86-87 -0.1400909567221973 1107 88-89 -0.17843241062365323 1107 90-91 -0.24368542355983536 1107 92-93 -0.1510848991240863 1107 94-95 -0.11392705732876962 1107 96-97 -0.15453489011621357 1107 98-99 -0.13380696501913647 1107 100-101 -0.11146468461300785 1108 1 -0.016196381369397272 1108 2 0.018330283653103407 1108 3 0.0038784945297862805 1108 4 0.021817836164359505 1108 5 0.012207897864144002 1108 6 0.029435371127526366 1108 7 0.0011883179511684716 1108 8 0.023190132776903738 1108 9 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52-53 -0.4034355022384446 2316 54-55 -0.4431253787092473 2316 56-57 -0.42025661690851734 2316 58-59 -0.4731119323232278 2316 60-61 -0.47199423018626874 2316 62-63 -0.4892237250092677 2316 64-65 -0.504710778511857 2316 66-67 -0.5278286779471273 2316 68-69 -0.44820711732539564 2316 70-71 -0.42394733132767115 2316 72-73 -0.2853437751557024 2316 74-75 -0.35422956011846907 2316 76-77 -0.36740664295505354 2316 78-79 -0.4058342404430739 2316 80-81 -0.45744622767264786 2316 82-83 -0.4747499187174249 2316 84-85 -0.4735819735115747 2316 86-87 -0.44929547816018456 2316 88-89 -0.499316081232724 2316 90-91 -0.526103221797726 2316 92-93 -0.594909352719462 2316 94-95 -0.6239832200097872 2316 96-97 -0.6838737156533305 2316 98-99 -0.6400036668237377 2316 100-101 -0.8415970559192942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 917346.0 3 173811.0 4 109424.0 5 112473.0 6 289319.0 7 273419.0 8 204124.0 9 329171.0 10 486979.0 11 445885.0 12 299856.0 13 222968.0 14 199506.0 15 182714.0 16 166285.0 17 150391.0 18 141280.0 19 135910.0 20 134531.0 21 138135.0 22 151701.0 23 171672.0 24 195106.0 25 222853.0 26 267216.0 27 376923.0 28 455444.0 29 286680.0 30 301354.0 31 349308.0 32 423259.0 33 538864.0 34 738491.0 35 1180333.0 36 2071332.0 37 5099514.0 38 1.2120801E7 39 5536402.0 40 27905.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.999113793294743 20.481798704677583 8.551445282353743 44.967642219673934 2 13.45424150595914 15.433618251916116 60.53836177550663 10.57377846661812 3 14.804585125721031 25.225960486613523 38.33220339173338 21.63725099593207 4 17.658761240266728 30.493644744181147 34.78910627997353 17.058487735578595 5 18.329420970266042 30.280328366704378 34.22728892051574 17.162961742513836 6 16.006218676832866 35.199566843117694 34.67003109310313 14.124183386946315 7 13.642656120421307 20.14916053070148 51.16589218052099 15.042291168356225 8 12.7356346990108 22.42322832531831 46.55348648697424 18.287650488696645 9 14.066053592437244 24.335594286129137 42.46476939479282 19.133582726640803 10-11 20.489512081181996 31.462117219154184 31.835739051074498 16.212631648589323 12-13 18.1089285728097 24.891641868220034 39.11064636460034 17.88878319436992 14-15 17.22010702157319 27.69872089669039 38.275324607678904 16.80584747405752 16-17 18.56993347759583 27.725831376064768 36.49348562398503 17.210749522354373 18-19 19.45660269302094 27.717496014700583 36.86228089291803 15.96362039936044 20-21 19.011078570265266 27.325638744419233 35.45013786799934 18.213144817316163 22-23 19.415637541854515 29.791430079853104 33.65815786014213 17.13477451815025 24-25 17.657927340940237 29.354014788327227 33.96785155548103 19.020206315251503 26-27 17.77919098357389 28.57449571021054 34.28732224833845 19.35899105787712 28-29 17.75157624309065 30.851399624871778 32.34359128006427 19.053432851973305 30-31 19.018371664888033 29.151380015869872 33.19412591980145 18.636122399440648 32-33 18.63805004911389 30.836972785524157 31.19478205162553 19.330195113736423 34-35 19.103559219605778 29.623449184790346 31.296207323070334 19.97678427253354 36-37 20.384627272336044 30.27457075445426 31.760060328283135 17.580741644926558 38-39 20.50076295554154 29.450870578790884 32.529128698143225 17.519237767524352 40-41 19.005402630642916 29.806602931203685 33.568877048047426 17.619117390105973 42-43 19.34369547334383 28.829722467743714 32.769073782301035 19.05750827661143 44-45 19.044727535680504 29.083200838056477 32.896903318854434 18.975168307408588 46-47 19.300666917205806 28.94127324745312 33.604381710199796 18.153678125141273 48-49 18.663835010139387 30.630714116738755 32.32483031633999 18.380620556781867 50-51 18.989846052232608 29.519997499942992 31.87461341320002 19.615543034624384 52-53 19.595058138540324 29.45708757794986 31.2061778998546 19.741676383655218 54-55 18.64023340803428 30.095696944871804 31.483717019792024 19.780352627301887 56-57 18.77858193303857 29.83488602776343 32.985202021101514 18.401330018096484 58-59 19.06570188604216 29.051598378302167 32.186415956162264 19.696283779493406 60-61 20.01171093818694 30.45504096448494 29.74810372138078 19.785144375947336 62-63 19.216031916968287 29.751378844388526 32.82109937908022 18.211489859562963 64-65 20.76017765560952 30.11889862635958 30.631241123038755 18.489682594992143 66-67 19.027363619616473 33.46574763634491 28.94453457893012 18.562354165108502 68-69 18.99059318505043 31.638033791255786 31.28260804353055 18.088764980163226 70-71 19.559698419481713 32.163179721143024 29.747322533083363 18.5297993262919 72-73 18.480911434780545 33.69332631964203 29.50321327201191 18.322548973565517 74-75 18.59627208206607 31.250625555770768 31.21378497086626 18.9393173912969 76-77 19.109978329743807 32.050381495883826 30.032305806265942 18.807334368106424 78-79 18.897181974954833 31.95240314018164 30.161800491810236 18.98861439305329 80-81 19.02534380519653 31.04102489441573 30.71992751358857 19.21370378679917 82-83 19.53081501117602 31.244091191514368 30.11852066734402 19.1065731299656 84-85 19.040733501488024 31.675611378757562 30.293877627803113 18.989777491951305 86-87 19.924874473956482 30.860035305421196 29.886591028873923 19.328499191748403 88-89 19.401794694234166 31.44078886028701 30.014258301977698 19.143158143501125 90-91 19.351946441445456 31.20278614605047 30.66998900343637 18.775278409067703 92-93 19.406042244666814 31.21550542031667 30.07963416746492 19.298818167551595 94-95 19.48339057157161 31.46455409402996 29.941196359415063 19.110858974983362 96-97 19.047365263096964 31.209802607027004 29.84296983441376 19.899862295462274 98-99 19.200386733088067 31.801912799637517 29.8763014918207 19.121398975453715 100-101 19.077198489285163 31.98032519429736 29.602168078000645 19.34030823841684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24025.0 1 29708.0 2 30238.5 3 28963.5 4 36908.5 5 45951.0 6 54642.0 7 62657.0 8 71357.5 9 79047.5 10 85699.0 11 92358.0 12 100340.0 13 108214.5 14 117036.0 15 127014.5 16 134763.0 17 143899.5 18 160574.5 19 178641.0 20 202453.0 21 236193.5 22 274147.0 23 324820.5 24 388192.0 25 452423.5 26 520323.5 27 607171.0 28 714725.0 29 809297.5 30 993159.0 31 1149854.5 32 1344177.0 33 1476113.0 34 1352645.5 35 1361806.0 36 1524366.5 37 1657410.5 38 1832261.0 39 1689484.5 40 1506195.0 41 1365029.0 42 1132733.5 43 1080735.0 44 986818.5 45 914534.5 46 872677.0 47 826649.0 48 775074.5 49 725457.0 50 647767.5 51 566265.5 52 500397.5 53 454675.0 54 420575.0 55 375233.5 56 339663.0 57 294015.5 58 250464.0 59 213996.0 60 168311.0 61 130243.0 62 102118.5 63 74753.0 64 50221.0 65 34977.0 66 25499.5 67 19595.5 68 14509.5 69 10415.5 70 11266.0 71 15814.0 72 15976.0 73 25233.0 74 25704.0 75 11279.0 76 6632.5 77 10914.0 78 9781.0 79 2385.0 80 1156.0 81 577.0 82 373.0 83 243.5 84 196.0 85 149.0 86 125.5 87 86.5 88 49.0 89 30.5 90 18.5 91 13.5 92 8.0 93 8.0 94 7.0 95 4.0 96 4.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0772130658204197 2 0.011897716685305674 3 0.0 4 0.022703616482056523 5 0.03755681692995405 6 0.12965957065213043 7 0.14890810592644663 8 0.15460295545569533 9 0.1780671950143543 10-11 0.17769811038493283 12-13 0.20450516206253472 14-15 0.20476899442120863 16-17 0.20719681346645263 18-19 0.21813182271532053 20-21 0.22557807002980884 22-23 0.18667542739789583 24-25 0.20244362091949228 26-27 0.2087531437099068 28-29 0.2276438212636812 30-31 0.23745192953374505 32-33 0.2664959428056354 34-35 0.25700920480225414 36-37 0.24645731381890743 38-39 0.27904341684235606 40-41 0.3024206478571971 42-43 0.30131479733254257 44-45 0.33909615244009145 46-47 0.32166918313151327 48-49 0.30878911191923025 50-51 0.3073225969468141 52-53 0.3247678099823218 54-55 0.3292599768978282 56-57 0.32244383984421543 58-59 0.32427242262800327 60-61 0.34400090825692836 62-63 0.36367887279589467 64-65 0.22016669994977361 66-67 0.12147375071518919 68-69 0.30641181396394507 70-71 0.05804873236270157 72-73 0.014112224461834615 74-75 0.029072080544089687 76-77 0.04503814833469156 78-79 0.041957765210812584 80-81 0.050937888950995526 82-83 0.07330610153026978 84-85 0.03556123387658007 86-87 0.007610440856854526 88-89 0.01172229623405972 90-91 0.007172591410544622 92-93 0.00531032790011756 94-95 0.011760187051528845 96-97 0.016949825681189187 98-99 0.002840407946574509 100-101 0.005913774252403646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.5628685E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.394236922561145 #Duplication Level Percentage of deduplicated Percentage of total 1 77.09895837102168 16.494733818723166 2 8.433611101574327 3.60861348039646 3 3.4047570542973524 2.185265372501968 4 2.0235936096617535 1.731729644803371 5 1.3414427845127819 1.4349572374963295 6 0.9666936439628969 1.2409003710285713 7 0.7307509322162709 1.0943701002652124 8 0.6200168669223783 1.0611830197537142 9 0.49083954340733366 0.9451023734356409 >10 3.8617571922082496 16.8663615292173 >50 0.5436836949352225 8.145574806953903 >100 0.41894744882335466 17.520379930306877 >500 0.03856463383074765 5.614116550289661 >1k 0.023298957376317483 9.520799008171998 >5k 0.0016810686122865392 2.4638385623183763 >10k+ 0.0014030966369399922 10.072074194337853 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG 656919 1.8437924385926678 TruSeq Adapter, Index 2 (100% over 49bp) CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 102936 0.28891327311125853 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 84701 0.2377326022557386 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 83551 0.23450486595281303 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC 82980 0.23290222471022995 TruSeq Adapter, Index 2 (100% over 50bp) GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74782 0.20989267496120048 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 72834 0.20442517033676658 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 69639 0.19545767686907334 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 67943 0.19069746750406308 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 67128 0.18840998481981583 No Hit ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT 66774 0.18741640338395874 No Hit CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT 62841 0.17637754522795326 No Hit ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT 57154 0.1604156875281813 No Hit CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC 56683 0.15909371900759178 No Hit ATTAAATCCTATAGTGAGTCGTATTAAATCCTATAGTGAGTCGTATTAAA 47767 0.1340689391146488 No Hit GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 46931 0.13172251515878286 No Hit CTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGACTCACT 46065 0.12929188938631891 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 45973 0.12903367048208486 No Hit ATACGACTCACTATAGGGATTAATACGACTCACTATAGGGATTAATACGA 43678 0.12259223151233338 No Hit CTCACTATAGGGAATTTAATACGACTCACTATAGGGAATTTAATACGACT 43480 0.1220364995227862 No Hit CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT 42378 0.11894348612641753 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 41062 0.1152498331049827 No Hit CTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAATACGACTCACT 39825 0.11177791153392273 No Hit GTCGTATTAAACCCTATAGTGAGTCGTATTAAACCCTATAGTGAGTCGTA 39187 0.10998721956760403 No Hit GTTTAATACGACTCACTATAGGGTTTAATACGACTCACTATAGGGTTTAA 38463 0.10795514906037088 No Hit CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA 36947 0.10370015059494898 No Hit ATACGACTCACTATAGGGGAATTGTAATACGACTCACTATAGGGGAATTG 36576 0.10265885479635299 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 36432 0.10225468607668231 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.091236317029382 0.0 0.0 2.8067272199352852E-6 0.0 2 2.0968581916509126 2.8067272199352852E-6 0.0 2.8067272199352852E-6 0.0 3 2.0972932343700026 2.8067272199352852E-6 0.0 2.8067272199352852E-6 0.0 4 2.1032434960762654 2.8067272199352852E-6 0.0 2.8067272199352852E-6 0.0 5 2.104209010239923 2.8067272199352852E-6 0.0 2.8067272199352852E-6 0.0 6 2.1048966584088076 2.8067272199352852E-6 0.0 2.8067272199352852E-6 0.0 7 2.1065021063786102 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 8 2.1178637381649086 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 9 2.1185569997882325 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 10-11 2.1240652019573556 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 12-13 2.1289362770475533 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 14-15 2.132199097440728 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 16-17 2.136177633274986 2.8067272199352852E-6 0.0 5.6134544398705704E-6 0.0 18-19 2.1399737318399485 2.8067272199352852E-6 2.8067272199352852E-6 7.016818049838214E-6 0.0 20-21 2.1447549916591084 4.210090829902928E-6 2.8067272199352852E-6 9.823545269773498E-6 0.0 22-23 2.1488654436727037 5.6134544398705704E-6 2.8067272199352852E-6 1.1226908879741141E-5 0.0 24-25 2.152013188249861 5.6134544398705704E-6 2.8067272199352852E-6 1.2630272489708783E-5 0.0 26-27 2.1549363946494235 8.420181659805856E-6 2.8067272199352852E-6 1.4033636099676426E-5 0.0 28-29 2.1577445252329692 8.420181659805856E-6 2.8067272199352852E-6 1.4033636099676426E-5 2.8067272199352852E-6 30-31 2.160597563452033 8.420181659805856E-6 2.8067272199352852E-6 1.4033636099676426E-5 2.8067272199352852E-6 32-33 2.1635319967604754 8.420181659805856E-6 2.8067272199352852E-6 1.4033636099676426E-5 2.8067272199352852E-6 34-35 2.166850951698049 8.420181659805856E-6 2.8067272199352852E-6 1.543699970964407E-5 2.8067272199352852E-6 36-37 2.169535586283917 9.823545269773498E-6 2.8067272199352852E-6 1.9647090539546997E-5 2.8067272199352852E-6 38-39 2.1724012547754707 1.1226908879741141E-5 5.6134544398705704E-6 1.9647090539546997E-5 4.210090829902928E-6 40-41 2.1753048140844937 1.1226908879741141E-5 5.6134544398705704E-6 1.9647090539546997E-5 5.6134544398705704E-6 42-43 2.1785255335693696 1.1226908879741141E-5 5.6134544398705704E-6 1.9647090539546997E-5 5.6134544398705704E-6 44-45 2.1817111689639965 1.2630272489708783E-5 5.6134544398705704E-6 2.2453817759482282E-5 5.6134544398705704E-6 46-47 2.1851844938986664 1.4033636099676426E-5 5.6134544398705704E-6 2.3857181369449924E-5 5.6134544398705704E-6 48-49 2.188524499290389 1.4033636099676426E-5 5.6134544398705704E-6 2.5260544979417567E-5 5.6134544398705704E-6 50-51 2.1923346314914514 1.4033636099676426E-5 5.6134544398705704E-6 2.5260544979417567E-5 5.6134544398705704E-6 52-53 2.1966640082282014 1.4033636099676426E-5 7.016818049838214E-6 2.5260544979417567E-5 5.6134544398705704E-6 54-55 2.2012249399605963 1.4033636099676426E-5 8.420181659805856E-6 2.5260544979417567E-5 7.016818049838214E-6 56-57 2.206177410140172 1.4033636099676426E-5 8.420181659805856E-6 2.5260544979417567E-5 8.420181659805856E-6 58-59 2.211421779950621 1.4033636099676426E-5 8.420181659805856E-6 2.5260544979417567E-5 8.420181659805856E-6 60-61 2.217035234390492 1.4033636099676426E-5 8.420181659805856E-6 2.5260544979417567E-5 8.420181659805856E-6 62-63 2.2232423116373785 1.4033636099676426E-5 1.1226908879741141E-5 2.666390858938521E-5 8.420181659805856E-6 64-65 2.2302787767777565 1.6840363319611712E-5 1.1226908879741141E-5 2.806727219935285E-5 8.420181659805856E-6 66-67 2.2379579824514995 1.6840363319611712E-5 1.1226908879741141E-5 3.227736302925578E-5 8.420181659805856E-6 68-69 2.247283333639734 1.6840363319611712E-5 1.1226908879741141E-5 3.648745385915871E-5 8.420181659805856E-6 70-71 2.256541323374691 1.6840363319611712E-5 1.1226908879741141E-5 3.648745385915871E-5 1.1226908879741141E-5 72-73 2.2670721639038884 1.6840363319611712E-5 1.1226908879741141E-5 4.0697544689061636E-5 1.1226908879741141E-5 74-75 2.2788477879551268 1.6840363319611712E-5 1.1226908879741141E-5 4.210090829902928E-5 1.1226908879741141E-5 76-77 2.2915552454433836 1.6840363319611712E-5 1.1226908879741141E-5 5.052108995883513E-5 1.1226908879741141E-5 78-79 2.3063607315285424 1.6840363319611712E-5 1.1226908879741141E-5 5.613454439870571E-5 1.1226908879741141E-5 80-81 2.3228699010362015 1.6840363319611712E-5 1.1226908879741141E-5 6.0344635228608637E-5 1.1226908879741141E-5 82-83 2.3414195612327537 1.6840363319611712E-5 1.1226908879741141E-5 6.174799883857628E-5 1.1226908879741141E-5 84-85 2.363532361635014 1.6840363319611712E-5 1.6840363319611712E-5 6.174799883857628E-5 1.1226908879741141E-5 86-87 2.388822377250241 1.6840363319611712E-5 1.6840363319611712E-5 6.174799883857628E-5 1.1226908879741141E-5 88-89 2.4183716014217196 1.6840363319611712E-5 1.6840363319611712E-5 6.315136244854392E-5 1.1226908879741141E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 96990 0.0 74.68285 1 GATCGGA 104800 0.0 69.32326 2 ATCGGAA 105185 0.0 68.98302 3 TCGGAAG 105535 0.0 68.679565 4 CGGAAGA 106050 0.0 68.22361 5 AGAGCAC 115810 0.0 62.405678 9 GAAGAGC 119695 0.0 60.37675 7 GGAAGAG 126900 0.0 57.16696 6 AAGAGCA 134880 0.0 53.789246 8 GTTTTTT 464925 0.0 48.060104 1 ACCGATG 93795 0.0 42.836124 32-33 CGATGTA 92635 0.0 42.479073 34-35 TATGCCG 94480 0.0 42.330215 46-47 TCACCGA 94660 0.0 42.13503 30-31 TGCCGTC 94275 0.0 42.04719 48-49 CCGTCTT 95375 0.0 41.67483 50-51 CTCGTAT 95780 0.0 41.20255 42-43 ATCTCGT 96805 0.0 40.44076 40-41 CGTCTGA 99910 0.0 40.420425 16-17 GTATCTC 96365 0.0 40.27371 38-39 >>END_MODULE