FastQCFastQC Report
Fri 27 May 2016
SRR1233086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50948328
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC13438562.6376842042784996Illumina Single End PCR Primer 1 (100% over 50bp)
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1637390.32138247991180396No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1378120.27049366566062777No Hit
ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT1109990.21786583457655373No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT1109830.2178344302093682No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT1048050.20570841892986166No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT882390.17319312225516018No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT834020.1636991895003895No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC793800.15580491669913094No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG786810.15443293840771377No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT720450.1414079771175219No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG705850.13854232861184376Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA682360.1339317749544205No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT673130.13212013552240615No Hit
GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG657170.12898754989565114No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT656480.1288521185621636No Hit
ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA649500.12748210304369556No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC649070.12739770380688448No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT648030.12719357542017867No Hit
ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT580100.11386045877697891No Hit
ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT580040.11384868213928433No Hit
GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA555510.10903400009515524No Hit
GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT541410.10626649023693183No Hit
ATTTAATACGACTCACTATAGGAGAATTTAATACGACTCACTATAGGAGA540910.10616835158947709No Hit
CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG517060.10148713810588643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG2089850.081.093461
GATCGGA2271500.074.731112
ATCGGAA2287100.074.5002443
TCGGAAG2288900.074.0014954
CGGAAGA2314150.073.266435
AGAGCGT2276600.072.867869
AAGAGCG2355400.072.059118
GAAGAGC2537900.066.765987
GGAAGAG2681600.063.080416
CGCGTGT503650.059.1154021
AGACGGC164400.056.0930679
AAGACGG180100.051.704938
GCGTGTT620250.047.584672
GTTTTTT6944350.044.0413741
CCGTATC2069450.043.41162548-49
CGCCGTA2062700.043.3275846-47
GTCGCCG2031450.043.29641744-45
TGGTCGC1965900.043.05639342-43
TCGGTGG2115450.042.4421338-39
TCTCGGT2100500.042.30063236-37
Produced by FastQC (version 0.11.5)