##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50948328 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.916778446586118 33.0 31.0 34.0 27.0 34.0 2 31.223999971107983 33.0 31.0 34.0 27.0 34.0 3 31.165635288365106 34.0 31.0 34.0 27.0 34.0 4 34.51427825070138 37.0 35.0 37.0 30.0 37.0 5 34.46098027004929 37.0 35.0 37.0 30.0 37.0 6 34.52020922060485 37.0 35.0 37.0 30.0 37.0 7 34.585237910064485 37.0 35.0 37.0 31.0 37.0 8 34.589884578744176 37.0 35.0 37.0 32.0 37.0 9 36.13035436609421 39.0 37.0 39.0 32.0 39.0 10-11 36.00072729570242 39.0 37.0 39.0 30.0 39.0 12-13 35.91113573933182 39.0 37.0 39.0 30.0 39.0 14-15 37.04260119193705 40.0 37.0 41.0 30.5 41.0 16-17 36.8094003595172 40.0 37.0 41.0 30.0 41.0 18-19 36.358511421611325 40.0 36.5 41.0 28.0 41.0 20-21 36.00379571828147 39.5 35.0 41.0 27.0 41.0 22-23 35.7031959616025 39.0 35.0 41.0 26.5 41.0 24-25 35.324847029327444 39.0 35.0 41.0 24.5 41.0 26-27 35.125948804443595 40.0 35.0 41.0 21.0 41.0 28-29 34.83216875144558 40.0 35.0 41.0 17.5 41.0 30-31 34.45646017078323 40.0 34.5 41.0 14.0 41.0 32-33 34.28118909024846 39.5 34.5 41.0 12.0 41.0 34-35 34.19315078406498 39.0 34.5 41.0 9.5 41.0 36-37 34.09005694946456 39.0 34.0 41.0 9.0 41.0 38-39 33.96349715342965 39.0 34.0 41.0 9.0 41.0 40-41 33.8298292536705 39.0 34.0 41.0 8.0 41.0 42-43 33.58900665003178 39.0 33.5 41.0 7.0 41.0 44-45 33.301053657737306 39.0 33.0 41.0 2.0 41.0 46-47 33.07672447072257 39.0 33.0 41.0 2.0 41.0 48-49 33.23134109523673 39.0 33.0 41.0 2.0 41.0 50-51 32.61390790881302 38.0 32.0 40.0 2.0 40.5 52-53 32.46985806874761 38.0 32.5 40.0 2.0 40.5 54-55 32.85941259936931 39.0 33.0 41.0 2.0 41.0 56-57 32.77019761080285 39.0 33.0 41.0 2.0 41.0 58-59 32.609036502630666 38.0 33.0 41.0 2.0 41.0 60-61 32.48587539516508 38.0 32.5 41.0 2.0 41.0 62-63 32.23929958015502 38.0 32.0 40.5 2.0 41.0 64-65 31.701412242615696 38.0 31.0 40.0 2.0 41.0 66-67 30.846157856249963 37.0 30.0 40.0 2.0 41.0 68-69 30.47022230052378 37.0 29.0 40.0 2.0 41.0 70-71 30.144462513077954 36.0 29.0 39.0 2.0 41.0 72-73 29.773806041289518 36.0 28.5 39.0 2.0 41.0 74-75 29.38773636496962 35.0 28.0 39.0 2.0 40.5 76-77 29.00887835808861 35.0 28.0 37.5 2.0 40.0 78-79 28.613814382682 35.0 27.0 37.0 2.0 39.0 80-81 28.209596377726076 35.0 26.0 37.0 2.0 39.0 82-83 27.807162562822473 35.0 26.0 36.0 2.0 39.0 84-85 27.423852947637457 34.0 26.0 36.0 2.0 37.5 86-87 27.044808928764063 34.0 25.0 35.5 2.0 37.0 88-89 26.69704118651352 34.0 24.5 35.0 2.0 36.5 90-91 26.40183016408311 34.0 23.5 35.0 2.0 36.0 92-93 26.062071428526565 34.0 20.5 35.0 2.0 36.0 94-95 25.73408891848227 34.0 19.0 35.0 2.0 36.0 96-97 25.353306972114964 34.0 7.5 35.0 2.0 35.0 98-99 24.89229921343052 34.0 2.0 35.0 2.0 35.0 100-101 22.776880185351715 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6357607355296473 1101 2 0.6511303222821461 1101 3 0.7386476172620888 1101 4 0.8101757747645735 1101 5 0.8216969415117887 1101 6 0.7113580618925113 1101 7 0.6969131496130601 1101 8 0.6294743535374607 1101 9 0.7984930847282072 1101 10-11 0.7379218473922506 1101 12-13 0.6618596058267627 1101 14-15 0.8827423328521817 1101 16-17 0.9685006366474838 1101 18-19 0.9379275788923067 1101 20-21 0.9669723296861434 1101 22-23 0.9485953617470884 1101 24-25 0.9025082081965508 1101 26-27 0.8201721348490238 1101 28-29 0.9107909286256657 1101 30-31 0.930760936665763 1101 32-33 0.8458388642947341 1101 34-35 0.853217651059289 1101 36-37 0.8468036295077042 1101 38-39 0.8613216781576156 1101 40-41 0.8959670805808528 1101 42-43 0.9291577111840468 1101 44-45 0.9233355919684527 1101 46-47 0.8405055156094505 1101 48-49 0.8140844558949993 1101 50-51 0.824645218150657 1101 52-53 0.9042643450450285 1101 54-55 1.007842552028663 1101 56-57 0.9561461183898885 1101 58-59 0.9827356463626487 1101 60-61 0.9792488554611722 1101 62-63 1.0201017755506356 1101 64-65 0.8573883646378668 1101 66-67 1.0227994655511239 1101 68-69 0.9951785943984284 1101 70-71 0.8744149582242713 1101 72-73 0.7963167980667336 1101 74-75 0.9276877350294299 1101 76-77 0.9655469166850921 1101 78-79 0.9049407990729357 1101 80-81 0.9365463819377453 1101 82-83 0.9780723500447657 1101 84-85 0.9946564941900213 1101 86-87 0.9498538550161513 1101 88-89 1.0131596379241614 1101 90-91 0.9782374408516468 1101 92-93 0.8471216166022053 1101 94-95 0.9383897825731111 1101 96-97 1.0090202761829907 1101 98-99 1.0668215523773696 1101 100-101 1.2272135261622132 1102 1 0.65778969106319 1102 2 0.6879269237726717 1102 3 0.7399467769996839 1102 4 0.7455189082836142 1102 5 0.7907277345781765 1102 6 0.7021238298327219 1102 7 0.6985139153672861 1102 8 0.6771898428047507 1102 9 0.8293348306335844 1102 10-11 0.8160348552026377 1102 12-13 0.8197313404912947 1102 14-15 0.9585515555250694 1102 16-17 1.0417202136952355 1102 18-19 0.9649649425788098 1102 20-21 0.9759484860638494 1102 22-23 0.9341524088899789 1102 24-25 0.9735250653858003 1102 26-27 0.9832593955306592 1102 28-29 1.0361608552701895 1102 30-31 1.0540131759830231 1102 32-33 0.9867448354768555 1102 34-35 0.9982286825999012 1102 36-37 0.9535584627628424 1102 38-39 0.9594179669959004 1102 40-41 1.0563510804997946 1102 42-43 1.0339632863673884 1102 44-45 1.1006103384445183 1102 46-47 1.0955564770800308 1102 48-49 1.1007411571292351 1102 50-51 1.126186661266452 1102 52-53 1.082899400745319 1102 54-55 1.217422457036875 1102 56-57 1.1524262825587996 1102 58-59 1.2337518128077747 1102 60-61 1.1841291259267805 1102 62-63 1.1683904683992807 1102 64-65 1.0016642650502767 1102 66-67 1.114721157503098 1102 68-69 1.1101935972316461 1102 70-71 1.0204685015054196 1102 72-73 1.048819185937603 1102 74-75 1.0564588155030883 1102 76-77 1.0486954636567525 1102 78-79 1.039297317361143 1102 80-81 0.9573104652989741 1102 82-83 1.0541931604415637 1102 84-85 1.081975729570381 1102 86-87 0.9771491921632212 1102 88-89 1.0694323288333614 1102 90-91 0.9737986028177126 1102 92-93 0.9501482116281821 1102 94-95 1.0083465601118924 1102 96-97 1.019826309833757 1102 98-99 1.0518991585551554 1102 100-101 1.1560459847413078 1103 1 0.5220834407384451 1103 2 0.6216974669026136 1103 3 0.6051203191036194 1103 4 0.6806416300458693 1103 5 0.7302995564987285 1103 6 0.6497104317858771 1103 7 0.6270162042240912 1103 8 0.6601407484268904 1103 9 0.7299055481419003 1103 10-11 0.7328363449066941 1103 12-13 0.8099495472684737 1103 14-15 0.9452051686045735 1103 16-17 0.9752733080649421 1103 18-19 0.9119589833397654 1103 20-21 0.9083630257495159 1103 22-23 0.8942738022721457 1103 24-25 0.9134204389207383 1103 26-27 0.8970365656815389 1103 28-29 0.7968013893736341 1103 30-31 0.8534916385756119 1103 32-33 0.9123310546395587 1103 34-35 0.8808753708052635 1103 36-37 0.9088268632959284 1103 38-39 0.9167573122066415 1103 40-41 0.9138099091962033 1103 42-43 0.8623946034035299 1103 44-45 0.961653767527828 1103 46-47 0.9288197181803071 1103 48-49 0.8459654247720465 1103 50-51 0.8879874327758159 1103 52-53 0.8903384126116265 1103 54-55 0.9360579642286595 1103 56-57 0.8913368041488283 1103 58-59 1.0670687244684913 1103 60-61 1.0249496045941058 1103 62-63 0.9947245555366635 1103 64-65 0.8700024483080391 1103 66-67 0.9533749216384635 1103 68-69 0.9821467263955093 1103 70-71 1.01543040996253 1103 72-73 0.9580619392269085 1103 74-75 0.9045426528971561 1103 76-77 0.956236556608598 1103 78-79 0.9509495044694347 1103 80-81 0.8505519880883021 1103 82-83 0.9338033195558211 1103 84-85 0.8942224167658601 1103 86-87 0.9058806262676597 1103 88-89 0.9590420868353853 1103 90-91 0.9658691577391956 1103 92-93 0.9238677283850798 1103 94-95 0.9310874755725855 1103 96-97 0.9426804269529647 1103 98-99 0.9753259242196854 1103 100-101 1.0995978001855597 1104 1 0.4498454055036234 1104 2 0.5818681529831586 1104 3 0.667027211431904 1104 4 0.6420596705444126 1104 5 0.6189168844316555 1104 6 0.6032638019963699 1104 7 0.6219192632173502 1104 8 0.5328921272906015 1104 9 0.6277190883826904 1104 10-11 0.611293379524092 1104 12-13 0.6325778369499488 1104 14-15 0.7909438111677289 1104 16-17 0.7962051992438006 1104 18-19 0.7586913932813744 1104 20-21 0.8023417173144267 1104 22-23 0.7610169303578047 1104 24-25 0.7403895631780131 1104 26-27 0.799113574121705 1104 28-29 0.6991021497489172 1104 30-31 0.6887892909339541 1104 32-33 0.7875751170503165 1104 34-35 0.743811824862604 1104 36-37 0.7207003376319676 1104 38-39 0.7129238352125213 1104 40-41 0.7413383751420497 1104 42-43 0.7568033764762774 1104 44-45 0.7852220416727249 1104 46-47 0.8153873276450696 1104 48-49 0.8646402460903886 1104 50-51 0.7501907616854311 1104 52-53 0.7587470786540891 1104 54-55 0.9048496247763609 1104 56-57 0.8483883018434994 1104 58-59 0.9409015028489875 1104 60-61 0.9057777259834339 1104 62-63 0.8689008270782921 1104 64-65 0.7043940361713403 1104 66-67 0.8582440108762093 1104 68-69 0.9370356404306897 1104 70-71 0.9711323145372255 1104 72-73 0.8734076140646607 1104 74-75 0.9119803095507137 1104 76-77 0.897111118105645 1104 78-79 0.9275004447050073 1104 80-81 0.9289971232879566 1104 82-83 0.8811805090198312 1104 84-85 0.9400690537341809 1104 86-87 0.9083177280915145 1104 88-89 0.9349967653883446 1104 90-91 0.95454175640673 1104 92-93 0.8841471375691761 1104 94-95 0.9344869974643508 1104 96-97 0.9680098100939354 1104 98-99 0.9949685967993069 1104 100-101 1.1260317336249521 1105 1 0.4697316022840923 1105 2 0.4623371483226464 1105 3 0.42363470418690596 1105 4 0.5165858821850975 1105 5 0.5070768716348937 1105 6 0.5249575525481518 1105 7 0.5319509270848997 1105 8 0.4990447065345549 1105 9 0.5819911357697265 1105 10-11 0.6194813905540073 1105 12-13 0.5301305007838124 1105 14-15 0.5534634147844102 1105 16-17 0.6266765387142996 1105 18-19 0.5631492168406709 1105 20-21 0.5818255604752807 1105 22-23 0.6044458596015261 1105 24-25 0.638715790015354 1105 26-27 0.6576890482336921 1105 28-29 0.6436323205501608 1105 30-31 0.5471544319997577 1105 32-33 0.5469991601566733 1105 34-35 0.5956584651695707 1105 36-37 0.5279372543686662 1105 38-39 0.6129031211424447 1105 40-41 0.6083738897282558 1105 42-43 0.6183984335542618 1105 44-45 0.6148485796170959 1105 46-47 0.5568266856855644 1105 48-49 0.6302062388194685 1105 50-51 0.5316094440586241 1105 52-53 0.5735303229507807 1105 54-55 0.681882691599192 1105 56-57 0.6473868006947114 1105 58-59 0.8042164476543476 1105 60-61 0.7654670789674967 1105 62-63 0.6860881963822507 1105 64-65 0.663536933998035 1105 66-67 0.6915112789600144 1105 68-69 0.7560453453581566 1105 70-71 0.7776461240481929 1105 72-73 0.6917186179691512 1105 74-75 0.711865932218906 1105 76-77 0.6866291215439908 1105 78-79 0.6961870040756395 1105 80-81 0.6708652898652758 1105 82-83 0.6218568312860029 1105 84-85 0.6053037655820717 1105 86-87 0.6617611598485951 1105 88-89 0.6894029689135515 1105 90-91 0.6809642241863081 1105 92-93 0.6513455483595152 1105 94-95 0.695669657449077 1105 96-97 0.7299664081522899 1105 98-99 0.7159473589126435 1105 100-101 0.8322478017486397 1106 1 0.36848543866230443 1106 2 0.43210251409356815 1106 3 0.4840480224753563 1106 4 0.5250459121246465 1106 5 0.5198871811034635 1106 6 0.5086208813999988 1106 7 0.4515978572169743 1106 8 0.43840555255217595 1106 9 0.5171528260858551 1106 10-11 0.49875313355293116 1106 12-13 0.5091453087502771 1106 14-15 0.5491115382300222 1106 16-17 0.5603932357719472 1106 18-19 0.5985643409523149 1106 20-21 0.5560817947733483 1106 22-23 0.5173139508413058 1106 24-25 0.5892017989211098 1106 26-27 0.602493017780084 1106 28-29 0.5616385228121317 1106 30-31 0.6034572274070342 1106 32-33 0.6168530243841772 1106 34-35 0.6112915753284369 1106 36-37 0.5086753764374166 1106 38-39 0.584908105890463 1106 40-41 0.5832375991172114 1106 42-43 0.5515316379325697 1106 44-45 0.5250390203676218 1106 46-47 0.5350809419102589 1106 48-49 0.5597064025529193 1106 50-51 0.5021340447451266 1106 52-53 0.6042555714171414 1106 54-55 0.7160474004457882 1106 56-57 0.6217142660163546 1106 58-59 0.7751069976832596 1106 60-61 0.7426007053244277 1106 62-63 0.690227268591741 1106 64-65 0.6171912078500945 1106 66-67 0.6772946322903053 1106 68-69 0.7309308496272706 1106 70-71 0.7418493258168759 1106 72-73 0.6850321931574754 1106 74-75 0.6827310078581199 1106 76-77 0.7007799315185572 1106 78-79 0.7239672164484325 1106 80-81 0.7524047291603786 1106 82-83 0.7374939779830818 1106 84-85 0.7123860227246155 1106 86-87 0.7198049148732437 1106 88-89 0.7724075188882793 1106 90-91 0.738240848832266 1106 92-93 0.7151713646161042 1106 94-95 0.7313685217860701 1106 96-97 0.6729877963080817 1106 98-99 0.7416273352413505 1106 100-101 0.7127028779145945 1107 1 0.38294857520077485 1107 2 0.4195391744380572 1107 3 0.42965731320707334 1107 4 0.4776139238001562 1107 5 0.487346040332838 1107 6 0.5057065856393095 1107 7 0.42740142229338574 1107 8 0.423606279071457 1107 9 0.441687231286636 1107 10-11 0.43917927243347066 1107 12-13 0.45517431233637495 1107 14-15 0.5335182115163164 1107 16-17 0.5430415978870187 1107 18-19 0.49326302386618437 1107 20-21 0.5676298480297532 1107 22-23 0.5896783974587052 1107 24-25 0.5587558876070702 1107 26-27 0.5736041882143255 1107 28-29 0.6019649790325232 1107 30-31 0.6110517981359749 1107 32-33 0.5528360279940117 1107 34-35 0.5886320398584033 1107 36-37 0.5213222174541272 1107 38-39 0.5670557848144782 1107 40-41 0.5916683585619538 1107 42-43 0.5764064339678825 1107 44-45 0.6081474590615485 1107 46-47 0.6454011076100556 1107 48-49 0.5990558723972583 1107 50-51 0.5386910300982279 1107 52-53 0.5207699491123492 1107 54-55 0.666562495257466 1107 56-57 0.6286588416428529 1107 58-59 0.6998766598618076 1107 60-61 0.6245047979964582 1107 62-63 0.6326896659112506 1107 64-65 0.5436338378953529 1107 66-67 0.538368365230987 1107 68-69 0.6266947421115923 1107 70-71 0.6266335524207207 1107 72-73 0.6162267654995119 1107 74-75 0.7158822748436222 1107 76-77 0.687710719556879 1107 78-79 0.6252225410109276 1107 80-81 0.5764014863088036 1107 82-83 0.6362105830201372 1107 84-85 0.6677201669927086 1107 86-87 0.6288918186773067 1107 88-89 0.6039890008949484 1107 90-91 0.631965299223598 1107 92-93 0.5969802186869053 1107 94-95 0.6132494435362368 1107 96-97 0.6600161910721916 1107 98-99 0.5939758023086021 1107 100-101 0.4939253191842319 1108 1 0.18085235502397978 1108 2 0.2872831632501729 1108 3 0.35948599965851713 1108 4 0.30815534344976214 1108 5 0.3101165116493192 1108 6 0.3298046151553109 1108 7 0.3397365476610119 1108 8 0.31020384809554713 1108 9 0.2616857477013994 1108 10-11 0.3035818208194456 1108 12-13 0.2629400161705604 1108 14-15 0.24877232169210117 1108 16-17 0.30323287576347013 1108 18-19 0.2922018899252592 1108 20-21 0.3260358948563322 1108 22-23 0.2796518117497371 1108 24-25 0.34963807988575013 1108 26-27 0.37065592307735074 1108 28-29 0.3933997725987126 1108 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2316 92-93 -3.0080302951109346 2316 94-95 -2.9405714224033694 2316 96-97 -2.8703596598800694 2316 98-99 -2.854986734691213 2316 100-101 -2.6015302342642492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 685398.0 3 171552.0 4 149074.0 5 160314.0 6 213865.0 7 326156.0 8 509155.0 9 785856.0 10 863471.0 11 658258.0 12 481171.0 13 390983.0 14 347749.0 15 321430.0 16 315112.0 17 320858.0 18 338924.0 19 367624.0 20 409748.0 21 465348.0 22 537233.0 23 637046.0 24 797572.0 25 694006.0 26 516040.0 27 574627.0 28 648872.0 29 742712.0 30 864627.0 31 1030121.0 32 1256254.0 33 1576285.0 34 2069477.0 35 2927708.0 36 4424985.0 37 7814131.0 38 1.1643096E7 39 3902511.0 40 8979.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.370031963813982 21.6974363563346 9.058099629673308 42.874432050178115 2 16.034623855950525 15.94367959321723 57.336381474589516 10.685315076242736 3 15.381926553212022 26.191892921363944 37.46896599413665 20.957214531287377 4 18.49123335895115 28.838241000117105 35.9045141304751 16.766011510456647 5 17.911465544971453 30.273774379779727 34.028279500333305 17.78648057491551 6 17.648607542191385 35.41778789433488 33.28961033077938 13.64399423269435 7 15.069518260193593 19.371289686241305 51.24192907788934 14.31726297567576 8 12.104622865070311 24.535531983309117 45.660391260953844 17.699453890666724 9 13.745910825768332 25.697452791020503 41.671956082233876 18.88468030097729 10-11 20.503090414369584 32.34230920704355 31.55935937753926 15.595241001047613 12-13 18.61084352684415 24.37046500087344 38.2573341897034 18.76135728257901 14-15 18.637884849844564 26.398836433346357 39.11180979229831 15.851468924510772 16-17 20.085739224047707 25.934329474440244 36.442267375043166 17.53766392646889 18-19 20.118116014408827 27.489134242893392 37.0229676926895 15.369782050008288 20-21 19.080999156559162 29.52210445852709 35.16771800467799 16.22917838023577 22-23 22.782683292485046 27.724441520113906 33.1478109376565 16.345064249744542 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29.101300857305993 30.952446004178597 20.248849923033575 50-51 19.351863204425698 29.16467280040884 31.955267128448778 19.528196866716684 52-53 17.965498146522744 31.108546191138597 33.188444852679034 17.737510809659625 54-55 17.89769099472806 30.16224960959616 31.72783648033406 20.212222915341723 56-57 18.220536249733527 31.443009788732446 32.76631186988615 17.570142091647874 58-59 18.084059206971197 31.442629598965638 32.36618189799083 18.107129296072333 60-61 18.504891078305867 34.2892073168037 29.075144502993673 18.13075710189677 62-63 18.331638969614612 33.809644911493486 30.028226345335973 17.830489773555932 64-65 18.959165186321513 32.79548834728958 30.28900534126686 17.956341125122048 66-67 18.703546698511087 33.91912278865619 29.062781142778206 18.31454937005451 68-69 19.243118913061988 32.299292273824 30.766026499568493 17.691562313545525 70-71 19.46964331692172 32.01444523004191 30.245663185027272 18.270248268009105 72-73 18.537674299915192 33.08052252789946 29.920628544133653 18.461174628051694 74-75 18.85081864849184 31.1056517623038 31.3310885001098 18.71244108909456 76-77 19.059963039714354 31.59633928454851 30.76220059760154 18.581497078135598 78-79 18.878604847546292 32.19707059873168 30.02198946933901 18.90233508438302 80-81 19.160878746725363 31.517950909000657 30.49663492135717 18.82453542291681 82-83 19.409736370847366 31.101742015567957 30.67637601361954 18.812145599965135 84-85 19.374311310369226 31.27815383150741 30.241722810003687 19.10581204811968 86-87 19.59245552288717 31.11414036972348 30.132404724016336 19.16099938337301 88-89 19.53681564512107 30.981173964615515 30.52166482599602 18.960345564267396 90-91 19.743732307764045 31.15096146727774 30.58223278342303 18.52307344153519 92-93 19.792133228649465 30.862406819686957 30.512457011423074 18.833002940240505 94-95 19.811015686490315 30.953192993892515 30.416282740022794 18.81950857959438 96-97 19.70726970169583 31.190904814635616 29.901425555828297 19.20039992784025 98-99 19.52656065159932 30.934172522613878 30.735931956640194 18.803334869146607 100-101 19.86141065839683 31.818619588451984 29.094669172684057 19.225300580467124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34153.0 1 39401.5 2 38266.0 3 36941.0 4 45317.0 5 55243.0 6 64558.5 7 74054.5 8 87072.0 9 97851.5 10 105977.0 11 114566.0 12 126846.5 13 137342.5 14 148693.0 15 160992.0 16 172651.0 17 183917.5 18 207242.0 19 240713.0 20 281785.5 21 331853.0 22 381877.0 23 452255.0 24 548595.5 25 637715.0 26 732706.5 27 866082.0 28 1180044.5 29 1384867.5 30 1503925.0 31 1733153.5 32 2133256.5 33 2231047.0 34 1934044.5 35 2031531.0 36 2181718.0 37 2248594.0 38 2479855.0 39 2312579.5 40 2143761.0 41 2019949.0 42 1696378.5 43 1589305.0 44 1444621.5 45 1307862.0 46 1218644.0 47 1142180.0 48 1074936.5 49 1014239.0 50 927486.5 51 832751.0 52 749832.0 53 676464.5 54 607503.0 55 533161.0 56 465818.0 57 384528.5 58 319121.0 59 264307.5 60 198965.0 61 141279.0 62 101963.0 63 78422.0 64 59759.0 65 43531.5 66 32720.0 67 27001.5 68 20293.5 69 12928.0 70 10266.5 71 10074.0 72 8787.5 73 10257.0 74 10478.5 75 6691.0 76 4890.5 77 4702.5 78 4136.0 79 2353.0 80 1310.0 81 815.0 82 525.0 83 356.5 84 250.0 85 157.5 86 108.5 87 78.5 88 55.0 89 36.5 90 21.0 91 19.5 92 16.0 93 7.5 94 5.5 95 3.5 96 1.5 97 1.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6388001584664368 2 0.28613892883786096 3 0.2857581508857366 4 0.3053211088693627 5 0.27757338768801204 6 0.21460370593515848 7 0.09484118890025203 8 0.10731068544585017 9 0.10336158627227178 10-11 0.08745233013338534 12-13 0.09616311648146726 14-15 0.09543492771735315 16-17 0.09180085360210447 18-19 0.10674049990413816 20-21 0.11263274429732023 22-23 0.08857896180616566 24-25 0.10509667755927142 26-27 0.09018058453262687 28-29 0.09806504346913994 30-31 0.10962970168520544 32-33 0.10563447734732334 34-35 0.10846970287229053 36-37 0.10789559178468035 38-39 0.10874154692574013 40-41 0.10305048675984028 42-43 0.09273120797997532 44-45 0.3703507208322911 46-47 0.08577415926190944 48-49 0.07167654255503733 50-51 0.07463640416227202 52-53 0.2274844034135919 54-55 0.29246690882574206 56-57 0.21696590317939385 58-59 0.25374434269952884 60-61 0.07213779419807456 62-63 0.06467631283209137 64-65 0.10091400840475079 66-67 0.0982073445079493 68-69 0.07908306627844588 70-71 0.10657072004404149 72-73 0.13163140505808157 74-75 0.11993131550852856 76-77 0.10805555778003156 78-79 0.10056856036571014 80-81 0.09617096757326364 82-83 0.10329877753790075 84-85 0.09019628671621961 86-87 0.11813734103305609 88-89 0.1261032550469566 90-91 0.15280187408701615 92-93 0.15876968523873836 94-95 0.11398313208629732 96-97 0.09842521230529881 98-99 0.11559751283692764 100-101 0.10813406869799534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.0948328E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.84419731011183 #Duplication Level Percentage of deduplicated Percentage of total 1 79.70434492103186 16.61373092007198 2 7.984253509720754 3.328507110611446 3 3.0936937159629254 1.9345668669775284 4 1.7701348908606602 1.4758816372245152 5 1.1044269718358615 1.151044685777801 6 0.7889720277115068 0.9867293170666601 7 0.5910167332507769 0.8623488581019847 8 0.46621810192787816 0.7774353684904417 9 0.39691486387350766 0.7446034564106015 >10 3.1130151700560837 13.388485879140019 >50 0.48521036221258307 7.100869379996704 >100 0.422329474412826 17.6930024978715 >500 0.04689166218259054 6.719391299379356 >1k 0.02847047939751313 11.126547398589727 >5k 0.0023060834738365117 3.3145049504770365 >10k+ 0.0018010320888726594 12.782350373812854 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 1343856 2.6376842042784996 Illumina Single End PCR Primer 1 (100% over 50bp) GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 163739 0.32138247991180396 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 137812 0.27049366566062777 No Hit ATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGTCGTAT 110999 0.21786583457655373 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 110983 0.2178344302093682 No Hit ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT 104805 0.20570841892986166 No Hit ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT 88239 0.17319312225516018 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83402 0.1636991895003895 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 79380 0.15580491669913094 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 78681 0.15443293840771377 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 72045 0.1414079771175219 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 70585 0.13854232861184376 Illumina Single End PCR Primer 1 (100% over 50bp) CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 68236 0.1339317749544205 No Hit GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT 67313 0.13212013552240615 No Hit GTCGTATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCG 65717 0.12898754989565114 No Hit ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT 65648 0.1288521185621636 No Hit ATTAAATTCCTATAGTGAGTCGTATTAAATTCCTATAGTGAGTCGTATTA 64950 0.12748210304369556 No Hit GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC 64907 0.12739770380688448 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 64803 0.12719357542017867 No Hit ATTAAATTCTCCTATAGTGAGTCGTATTAAATTCTCCTATAGTGAGTCGT 58010 0.11386045877697891 No Hit ATTTAATACGACTCACTATAGGGATTTAATACGACTCACTATAGGGATTT 58004 0.11384868213928433 No Hit GGATAATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATA 55551 0.10903400009515524 No Hit GTCGTATTAAATTCTCTATAGTGAGTCGTATTAAATTCTCTATAGTGAGT 54141 0.10626649023693183 No Hit ATTTAATACGACTCACTATAGGAGAATTTAATACGACTCACTATAGGAGA 54091 0.10616835158947709 No Hit CTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTG 51706 0.10148713810588643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.358084685330596 0.0 0.0 0.0 0.0 2 3.3988750327586805 0.0 0.0 0.0 0.0 3 3.399065421734743 0.0 0.0 0.0 0.0 4 3.4147852702840416 0.0 0.0 0.0 0.0 5 3.4154545758596826 0.0 0.0 3.92554589818924E-6 0.0 6 3.4160473332903094 0.0 0.0 3.92554589818924E-6 0.0 7 3.417240699243359 0.0 0.0 3.92554589818924E-6 0.0 8 3.4233959552117197 0.0 0.0 5.88831884728386E-6 0.0 9 3.4239690849128555 0.0 0.0 9.8138647454731E-6 0.0 10-11 3.4285963221403457 3.92554589818924E-6 0.0 9.8138647454731E-6 0.0 12-13 3.431756386588388 3.92554589818924E-6 0.0 9.8138647454731E-6 0.0 14-15 3.4340714772818455 3.92554589818924E-6 0.0 1.275802416911503E-5 9.8138647454731E-7 16-17 3.4369243677633543 3.92554589818924E-6 0.0 1.864634301639889E-5 1.96277294909462E-6 18-19 3.4393974616792136 3.92554589818924E-6 0.0 1.96277294909462E-5 1.96277294909462E-6 20-21 3.446751971919471 3.92554589818924E-6 0.0 2.060911596549351E-5 1.96277294909462E-6 22-23 3.4539838088504102 5.88831884728386E-6 0.0 2.2571888914588128E-5 1.96277294909462E-6 24-25 3.456457884152744 6.8697053218311695E-6 0.0 2.453466186368275E-5 1.96277294909462E-6 26-27 3.458823025556403 7.85109179637848E-6 0.0 2.551604833823006E-5 1.96277294909462E-6 28-29 3.460938894795527 8.832478270925789E-6 0.0 2.7478821287324678E-5 1.96277294909462E-6 30-31 3.463481667151079 1.668357006730427E-5 1.96277294909462E-6 2.94415942364193E-5 1.96277294909462E-6 32-33 3.466965589135722 2.3553275389135442E-5 1.96277294909462E-6 3.042298071096661E-5 1.96277294909462E-6 34-35 3.470064807622342 2.551604833823006E-5 1.96277294909462E-6 3.140436718551392E-5 1.96277294909462E-6 36-37 3.4731169195581844 2.551604833823006E-5 1.96277294909462E-6 3.5329913083703156E-5 1.96277294909462E-6 38-39 3.4768216534996004 2.551604833823006E-5 1.96277294909462E-6 3.729268603279778E-5 1.96277294909462E-6 40-41 3.4806284516343697 2.551604833823006E-5 1.96277294909462E-6 4.5143777829176256E-5 1.96277294909462E-6 42-43 3.4857974141958104 2.551604833823006E-5 1.96277294909462E-6 5.103209667646012E-5 1.96277294909462E-6 44-45 3.489879981929927 2.551604833823006E-5 1.96277294909462E-6 5.88831884728386E-5 1.96277294909462E-6 46-47 3.4993866727088667 2.551604833823006E-5 1.96277294909462E-6 6.673428026921708E-5 3.92554589818924E-6 48-49 3.5047087315603367 2.551604833823006E-5 1.96277294909462E-6 6.86970532183117E-5 3.92554589818924E-6 50-51 3.511095594736691 2.551604833823006E-5 1.96277294909462E-6 7.262259911650094E-5 3.92554589818924E-6 52-53 3.5228486791558695 2.649743481277737E-5 1.96277294909462E-6 7.556675854014286E-5 3.92554589818924E-6 54-55 3.5300186887389122 2.7478821287324678E-5 1.96277294909462E-6 7.85109179637848E-5 3.92554589818924E-6 56-57 3.5367549255002046 2.7478821287324678E-5 1.96277294909462E-6 8.047369091287941E-5 3.92554589818924E-6 58-59 3.543682532624034 2.7478821287324678E-5 1.96277294909462E-6 8.439923681106867E-5 3.92554589818924E-6 60-61 3.5500124753848645 2.94415942364193E-5 1.96277294909462E-6 8.93061691838052E-5 3.92554589818924E-6 62-63 3.5581589645100813 3.042298071096661E-5 1.96277294909462E-6 9.519448803108906E-5 3.92554589818924E-6 64-65 3.5658059279197545 3.140436718551392E-5 1.96277294909462E-6 1.0108280687837293E-4 5.88831884728386E-6 66-67 3.5743449716347904 3.140436718551392E-5 1.96277294909462E-6 1.0402696630201485E-4 5.88831884728386E-6 68-69 3.5841431341966707 3.140436718551392E-5 1.96277294909462E-6 1.0500835277656216E-4 5.88831884728386E-6 70-71 3.5935477607822577 3.434852660915585E-5 1.96277294909462E-6 1.1089667162384603E-4 5.88831884728386E-6 72-73 3.6041104234078105 3.729268603279778E-5 1.96277294909462E-6 1.26598855216603E-4 5.88831884728386E-6 74-75 3.6160342690735603 3.729268603279778E-5 1.96277294909462E-6 1.4328242528390727E-4 5.88831884728386E-6 76-77 3.6294930031855017 3.729268603279778E-5 3.92554589818924E-6 1.6389154124940076E-4 5.88831884728386E-6 78-79 3.6434188772593283 4.121823193098702E-5 3.92554589818924E-6 1.687984736221373E-4 5.88831884728386E-6 80-81 3.659131856103305 4.318100488008164E-5 3.92554589818924E-6 1.7370540599487386E-4 5.88831884728386E-6 82-83 3.6762982683160867 4.318100488008164E-5 3.92554589818924E-6 2.1884918382405013E-4 5.88831884728386E-6 84-85 3.69631168269153 4.6125164303723563E-5 3.92554589818924E-6 2.24737502671334E-4 5.88831884728386E-6 86-87 3.719003300756013 4.710655077827088E-5 3.92554589818924E-6 2.267002756204286E-4 5.88831884728386E-6 88-89 3.7453486206652356 4.710655077827088E-5 3.92554589818924E-6 2.4043968626409097E-4 5.88831884728386E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 208985 0.0 81.09346 1 GATCGGA 227150 0.0 74.73111 2 ATCGGAA 228710 0.0 74.500244 3 TCGGAAG 228890 0.0 74.001495 4 CGGAAGA 231415 0.0 73.26643 5 AGAGCGT 227660 0.0 72.86786 9 AAGAGCG 235540 0.0 72.05911 8 GAAGAGC 253790 0.0 66.76598 7 GGAAGAG 268160 0.0 63.08041 6 CGCGTGT 50365 0.0 59.115402 1 AGACGGC 16440 0.0 56.093067 9 AAGACGG 18010 0.0 51.70493 8 GCGTGTT 62025 0.0 47.58467 2 GTTTTTT 694435 0.0 44.041374 1 CCGTATC 206945 0.0 43.411625 48-49 CGCCGTA 206270 0.0 43.32758 46-47 GTCGCCG 203145 0.0 43.296417 44-45 TGGTCGC 196590 0.0 43.056393 42-43 TCGGTGG 211545 0.0 42.44213 38-39 TCTCGGT 210050 0.0 42.300632 36-37 >>END_MODULE